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The map label for this gene is dctD [H]

Identifier: 186474996

GI number: 186474996

Start: 251706

End: 253064

Strand: Direct

Name: dctD [H]

Synonym: Bphy_0227

Alternate gene names: 186474996

Gene position: 251706-253064 (Clockwise)

Preceding gene: 186474995

Following gene: 186474998

Centisome position: 7.23

GC content: 65.86

Gene sequence:

>1359_bases
ATGGTGAACAAGGGCTTGCAAGTCCTCTATATCGAAGACGACGAACTCGTGCGGCGCGCAAGCGTGCAGAGCCTGCAACT
CGCAGGATTCGAAGTGGTCGGGTATGCGTCGGTGGAGTCGGCGGCGAAGCTGATCCACGCGGAGTTTTCGGGCGTGATCG
TCAGTGATATTCGTTTGCCGGGCGCGAGCGGCCTCGATCTGCTCGCGCAATGCCGCGAGCGCGCGCCCGAGGTGCCCGTG
ATTCTCGTCACGGGCCACGGCGATATCTCGATGGCCGTGCAGGCGATGCGCGATGGTGCGTATGACTTCATCGAAAAGCC
GTTCGCTTCCGAGCGGCTGATCGAAACCGTGCGCCGCGCCGTCGAGCGCCGCACGCTGGTGCTCGAAAATCTCGCGCTGC
GCCGCGAACTCGCAGGGCAGGGCACACTCGCGCCACGCATCATCGGACGCAGTCCCGCGATCGAACAGGTGCGCAAGCTG
ATCGCGAACGTCGCGCCGACGGATGCATCGGTACTGATTAACGGCGATACGGGCGCGGGCAAGGAGCTGATCGCGCGCAG
TCTGCATGAGCTGTCGCCGCGCCGCGACAAGCCGTTTCTCGCGGTGAACTGCGGTGCGCTGCCGGAGCCGATGTTCGAGT
CGGAAATGTTCGGTTATGAGCCGGGCGCATTCACTGGCGCGGCGAAGCGGCGCATCGGCAAGCTGGAGCACGCGTCGGGC
GGCACACTGTTTCTCGACGAGATCGAAAGCATGCCGCTCGCGTTACAGGTGAAGCTGCTGCGCGTGCTGCAGGACGGCGT
GCTGGAGCGGCTCGGATCGAATCAGCCGGTTCGCGTCGATTGCCGGATCGTCGCGGCGGCAAAGGGCGACATGACCGATC
ATGTCGCAGCGGGCACGTTCCGGCGCGACCTGCTATACCGGCTCAACGTGGTGACGATCGCGCTGCCGCCGCTGTCCGAG
CGGCGCGAGGACATCGTGCCGTTGTTCGAGCATTTCCTGCTTGATGCGGCCGTGCGTTATCAGCGTCCCGCGCCGATTCT
CACCGACCGCCAACGCACGAATCTGATGCAGCGCGAGTGGCCCGGCAACGTGCGAGAGTTGCGCAATGCGGCGGACCGGT
TCGTGCTGGGCGTCGGCGATGACACCGCGACGGCGACGGGCGACGACGCGCTGACCTCACCCATGCAGCCGTTGAAAGAG
CGTGTAGAGCAGTTCGAGCGCGCGGTGATCGCGGAGGCGCTCGAGCAGACAGGCGGCGCCGTCGCGGTGGCGGCGGACAG
ACTGCAGGTCGGCAAGGCGACGCTCTACGAGAAGATCAAGCGCTATGGCCTCGCGGCGCGCGGGGAGGGTGAGCGGTAA

Upstream 100 bases:

>100_bases
TCGTGCTGACGCTTCGGCGCGTGCGCGCGCAGGCAACGGATATTGCGGACTCTGTGACGACGGGATCCTGAATCAGCAAG
TTTTGACGGCGGTATACGAC

Downstream 100 bases:

>100_bases
GCAGTAAGCAGAAACGGGGGGCGGTGCCGACGGCGGTTGCGCCAGGCAGTCAGCGCACAGATGCCCGCCGTCCGGAGAGA
CCGGTCAGGTTGTGCCCAAG

Product: two component, sigma54 specific, Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MVNKGLQVLYIEDDELVRRASVQSLQLAGFEVVGYASVESAAKLIHAEFSGVIVSDIRLPGASGLDLLAQCRERAPEVPV
ILVTGHGDISMAVQAMRDGAYDFIEKPFASERLIETVRRAVERRTLVLENLALRRELAGQGTLAPRIIGRSPAIEQVRKL
IANVAPTDASVLINGDTGAGKELIARSLHELSPRRDKPFLAVNCGALPEPMFESEMFGYEPGAFTGAAKRRIGKLEHASG
GTLFLDEIESMPLALQVKLLRVLQDGVLERLGSNQPVRVDCRIVAAAKGDMTDHVAAGTFRRDLLYRLNVVTIALPPLSE
RREDIVPLFEHFLLDAAVRYQRPAPILTDRQRTNLMQREWPGNVRELRNAADRFVLGVGDDTATATGDDALTSPMQPLKE
RVEQFERAVIAEALEQTGGAVAVAADRLQVGKATLYEKIKRYGLAARGEGER

Sequences:

>Translated_452_residues
MVNKGLQVLYIEDDELVRRASVQSLQLAGFEVVGYASVESAAKLIHAEFSGVIVSDIRLPGASGLDLLAQCRERAPEVPV
ILVTGHGDISMAVQAMRDGAYDFIEKPFASERLIETVRRAVERRTLVLENLALRRELAGQGTLAPRIIGRSPAIEQVRKL
IANVAPTDASVLINGDTGAGKELIARSLHELSPRRDKPFLAVNCGALPEPMFESEMFGYEPGAFTGAAKRRIGKLEHASG
GTLFLDEIESMPLALQVKLLRVLQDGVLERLGSNQPVRVDCRIVAAAKGDMTDHVAAGTFRRDLLYRLNVVTIALPPLSE
RREDIVPLFEHFLLDAAVRYQRPAPILTDRQRTNLMQREWPGNVRELRNAADRFVLGVGDDTATATGDDALTSPMQPLKE
RVEQFERAVIAEALEQTGGAVAVAADRLQVGKATLYEKIKRYGLAARGEGER
>Mature_452_residues
MVNKGLQVLYIEDDELVRRASVQSLQLAGFEVVGYASVESAAKLIHAEFSGVIVSDIRLPGASGLDLLAQCRERAPEVPV
ILVTGHGDISMAVQAMRDGAYDFIEKPFASERLIETVRRAVERRTLVLENLALRRELAGQGTLAPRIIGRSPAIEQVRKL
IANVAPTDASVLINGDTGAGKELIARSLHELSPRRDKPFLAVNCGALPEPMFESEMFGYEPGAFTGAAKRRIGKLEHASG
GTLFLDEIESMPLALQVKLLRVLQDGVLERLGSNQPVRVDCRIVAAAKGDMTDHVAAGTFRRDLLYRLNVVTIALPPLSE
RREDIVPLFEHFLLDAAVRYQRPAPILTDRQRTNLMQREWPGNVRELRNAADRFVLGVGDDTATATGDDALTSPMQPLKE
RVEQFERAVIAEALEQTGGAVAVAADRLQVGKATLYEKIKRYGLAARGEGER

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. When activated by dctB acts in conjunction with sigma-54 to activate the transcription of dctA [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=452, Percent_Identity=38.9380530973451, Blast_Score=301, Evalue=7e-83,
Organism=Escherichia coli, GI1790299, Length=476, Percent_Identity=38.655462184874, Blast_Score=298, Evalue=5e-82,
Organism=Escherichia coli, GI1790437, Length=450, Percent_Identity=37.3333333333333, Blast_Score=283, Evalue=2e-77,
Organism=Escherichia coli, GI1788905, Length=450, Percent_Identity=37.3333333333333, Blast_Score=271, Evalue=6e-74,
Organism=Escherichia coli, GI1789087, Length=338, Percent_Identity=44.0828402366864, Blast_Score=238, Evalue=5e-64,
Organism=Escherichia coli, GI87082117, Length=321, Percent_Identity=42.6791277258567, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1789233, Length=320, Percent_Identity=39.6875, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI87082152, Length=320, Percent_Identity=42.1875, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI87081872, Length=251, Percent_Identity=44.6215139442231, Blast_Score=214, Evalue=6e-57,
Organism=Escherichia coli, GI1786524, Length=331, Percent_Identity=39.2749244712991, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1787583, Length=320, Percent_Identity=36.875, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI87081858, Length=306, Percent_Identity=30.718954248366, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI1789828, Length=243, Percent_Identity=35.3909465020576, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1789402, Length=122, Percent_Identity=31.9672131147541, Blast_Score=63, Evalue=5e-11,
Organism=Escherichia coli, GI1789809, Length=123, Percent_Identity=28.4552845528455, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 49482; Mature: 49482

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNKGLQVLYIEDDELVRRASVQSLQLAGFEVVGYASVESAAKLIHAEFSGVIVSDIRLP
CCCCCCEEEEECCHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHCCEEEEEEECC
GASGLDLLAQCRERAPEVPVILVTGHGDISMAVQAMRDGAYDFIEKPFASERLIETVRRA
CCCCHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHH
VERRTLVLENLALRRELAGQGTLAPRIIGRSPAIEQVRKLIANVAPTDASVLINGDTGAG
HHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCC
KELIARSLHELSPRRDKPFLAVNCGALPEPMFESEMFGYEPGAFTGAAKRRIGKLEHASG
HHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHCCCCCCCCCHHHHHHHHCCCCCCC
GTLFLDEIESMPLALQVKLLRVLQDGVLERLGSNQPVRVDCRIVAAAKGDMTDHVAAGTF
CEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCHHHHHHHH
RRDLLYRLNVVTIALPPLSERREDIVPLFEHFLLDAAVRYQRPAPILTDRQRTNLMQREW
HHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCC
PGNVRELRNAADRFVLGVGDDTATATGDDALTSPMQPLKERVEQFERAVIAEALEQTGGA
CCCHHHHHHHHHHEEEECCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCE
VAVAADRLQVGKATLYEKIKRYGLAARGEGER
EEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MVNKGLQVLYIEDDELVRRASVQSLQLAGFEVVGYASVESAAKLIHAEFSGVIVSDIRLP
CCCCCCEEEEECCHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHCCEEEEEEECC
GASGLDLLAQCRERAPEVPVILVTGHGDISMAVQAMRDGAYDFIEKPFASERLIETVRRA
CCCCHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHH
VERRTLVLENLALRRELAGQGTLAPRIIGRSPAIEQVRKLIANVAPTDASVLINGDTGAG
HHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCC
KELIARSLHELSPRRDKPFLAVNCGALPEPMFESEMFGYEPGAFTGAAKRRIGKLEHASG
HHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHCCCCCCCCCHHHHHHHHCCCCCCC
GTLFLDEIESMPLALQVKLLRVLQDGVLERLGSNQPVRVDCRIVAAAKGDMTDHVAAGTF
CEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCHHHHHHHH
RRDLLYRLNVVTIALPPLSERREDIVPLFEHFLLDAAVRYQRPAPILTDRQRTNLMQREW
HHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCC
PGNVRELRNAADRFVLGVGDDTATATGDDALTSPMQPLKERVEQFERAVIAEALEQTGGA
CCCHHHHHHHHHHEEEECCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCE
VAVAADRLQVGKATLYEKIKRYGLAARGEGER
EEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2134335; 2793824; 11481431 [H]