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The map label for this gene is dctA

Identifier: 186474994

GI number: 186474994

Start: 248341

End: 249621

Strand: Direct

Name: dctA

Synonym: Bphy_0225

Alternate gene names: 186474994

Gene position: 248341-249621 (Clockwise)

Preceding gene: 186474993

Following gene: 186474995

Centisome position: 7.14

GC content: 61.51

Gene sequence:

>1281_bases
GTGAAGAAACCTATTTATAAAGTCCTCTACGTGCAGGTGATCGTCGCGATCATCATCGGTATCGCGCTGGGACACTTCTA
CCCGGCGCTCGCCACCGACATGAAGCCGCTCGGCGACGCCTTCATCAAGCTGATCAAGATGGTCATCGGGCCGATCATCT
TCTGTACCGTCGTGACGGGTATCGCCGGCATGGAAGACATGAAGAAGGTCGGGCGCGTCGGCGGCAAGGCGCTGCTGTAC
TTCGAAATCGTGTCGACGTTCGCGCTGATTCTCGGCCTCGCCGCGACGCACCTGCTGCGTCCTGGCGTCGGGTTCAACGT
CGATCCCGCCACGCTCAACGGCAAGGAAGTTGCATCGTATGCTGCGAAGGCGCATGGGCAGACGACGGTCGATTTCCTGA
TGCACATCATTCCGGACACACTGGTGTCGGCGTTCTCGCAAGGTGAGATCCTGCAGATCCTGCTGATCGCGCTGCTGTTT
GGCGCGGTGCTCGCGCATGTCGGCGAGCGCGGCAAGCCGGTGACGAACTTCATCGAAAGCCTGTCGAGCATTCTGTTCGG
CATGGTCGGCATCATCACGAAGCTCGCGCCGATCGGTGCGTTCGGCGCGATGGCGTTCACCATCGGCAAATACGGCATCG
GTTCGCTGCTGCCGATGCTGAAGCTGATCGGCACGTTCTATCTGACTTCCATCGTGTTCGTCGTGGTGGTGCTCGGCTTC
ATCGCGAAGATGGTGGGCTTCAACATCCTGCGCTTCGTGGCGTACATCAAGGAGGAGATGCTGATCGTGCTGGGCACGAG
TTCGTCGGAAGCGGCGCTGCCGCAACTGATGCTGAAGCTCGAAAAGCTCGGCTGCTCGCGCTCGGTGGTCGGCCTCGTCG
TGCCGACGGGCTACTCGTTCAACCTCGACGGCACCAACATCTACATGACGATGGCCGTGCTGTTCATCGCGCAGGCGACC
AACACCGACCTGACGTGGGGCCAGCAGCTAACGCTGCTCGCGGTGACGATGCTGACGTCGAAGGGCGCAAGCGGCGTGAC
TGGCGCAGGCTTCATCACGCTGGCGGCGACGCTCGCCGTCGTGCCGACGATCCCGCTGTCGGGCATGGTGCTGATTCTCG
GTATCGACCGGTTCATGAGCGAATGCCGCGCGTTGACGAACATCGTCGGCAACGGCGTGGCGACGGTTGTCGTGTCGGCG
TGGGAAAAGGAACTCGACCGCTCGAAGCTGCGCGCCGCGTTGAGTCGCGATGTGTCCGTCACCGAGTCGGCTGAGGTCTA
A

Upstream 100 bases:

>100_bases
GTAGTCGCCCGCGCTGATGACGGACGTTGAAACGTTCGCCATGCGCTGCGCGACCGGCCTTCGGTCCACCCTGGCTTTAC
TCCATTGCAGGAACACCATC

Downstream 100 bases:

>100_bases
GCGATGAAGGCGGCGGCGCGGCAACATGGCGCGTCGCCGCACGCTACGCAAGCAACGGCGGCGCCCGGTGAGACGGGCCA
AGCGCACGCTGGCCCCGAAG

Product: C4-dicarboxylate transporter DctA

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MKKPIYKVLYVQVIVAIIIGIALGHFYPALATDMKPLGDAFIKLIKMVIGPIIFCTVVTGIAGMEDMKKVGRVGGKALLY
FEIVSTFALILGLAATHLLRPGVGFNVDPATLNGKEVASYAAKAHGQTTVDFLMHIIPDTLVSAFSQGEILQILLIALLF
GAVLAHVGERGKPVTNFIESLSSILFGMVGIITKLAPIGAFGAMAFTIGKYGIGSLLPMLKLIGTFYLTSIVFVVVVLGF
IAKMVGFNILRFVAYIKEEMLIVLGTSSSEAALPQLMLKLEKLGCSRSVVGLVVPTGYSFNLDGTNIYMTMAVLFIAQAT
NTDLTWGQQLTLLAVTMLTSKGASGVTGAGFITLAATLAVVPTIPLSGMVLILGIDRFMSECRALTNIVGNGVATVVVSA
WEKELDRSKLRAALSRDVSVTESAEV

Sequences:

>Translated_426_residues
MKKPIYKVLYVQVIVAIIIGIALGHFYPALATDMKPLGDAFIKLIKMVIGPIIFCTVVTGIAGMEDMKKVGRVGGKALLY
FEIVSTFALILGLAATHLLRPGVGFNVDPATLNGKEVASYAAKAHGQTTVDFLMHIIPDTLVSAFSQGEILQILLIALLF
GAVLAHVGERGKPVTNFIESLSSILFGMVGIITKLAPIGAFGAMAFTIGKYGIGSLLPMLKLIGTFYLTSIVFVVVVLGF
IAKMVGFNILRFVAYIKEEMLIVLGTSSSEAALPQLMLKLEKLGCSRSVVGLVVPTGYSFNLDGTNIYMTMAVLFIAQAT
NTDLTWGQQLTLLAVTMLTSKGASGVTGAGFITLAATLAVVPTIPLSGMVLILGIDRFMSECRALTNIVGNGVATVVVSA
WEKELDRSKLRAALSRDVSVTESAEV
>Mature_426_residues
MKKPIYKVLYVQVIVAIIIGIALGHFYPALATDMKPLGDAFIKLIKMVIGPIIFCTVVTGIAGMEDMKKVGRVGGKALLY
FEIVSTFALILGLAATHLLRPGVGFNVDPATLNGKEVASYAAKAHGQTTVDFLMHIIPDTLVSAFSQGEILQILLIALLF
GAVLAHVGERGKPVTNFIESLSSILFGMVGIITKLAPIGAFGAMAFTIGKYGIGSLLPMLKLIGTFYLTSIVFVVVVLGF
IAKMVGFNILRFVAYIKEEMLIVLGTSSSEAALPQLMLKLEKLGCSRSVVGLVVPTGYSFNLDGTNIYMTMAVLFIAQAT
NTDLTWGQQLTLLAVTMLTSKGASGVTGAGFITLAATLAVVPTIPLSGMVLILGIDRFMSECRALTNIVGNGVATVVVSA
WEKELDRSKLRAALSRDVSVTESAEV

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family

Homologues:

Organism=Homo sapiens, GI169790839, Length=481, Percent_Identity=25.987525987526, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI4827012, Length=275, Percent_Identity=30.1818181818182, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI21314632, Length=397, Percent_Identity=25.6926952141058, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI194239697, Length=489, Percent_Identity=23.721881390593, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI40254478, Length=447, Percent_Identity=25.7270693512304, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI66773030, Length=415, Percent_Identity=25.3012048192771, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI5032093, Length=404, Percent_Identity=26.2376237623762, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI262359914, Length=403, Percent_Identity=25.3101736972705, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI223468566, Length=279, Percent_Identity=27.5985663082437, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI223468564, Length=279, Percent_Identity=27.5985663082437, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI301601644, Length=167, Percent_Identity=28.7425149700599, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1789947, Length=426, Percent_Identity=61.2676056338028, Blast_Score=530, Evalue=1e-152,
Organism=Escherichia coli, GI1790514, Length=421, Percent_Identity=35.8669833729216, Blast_Score=285, Evalue=5e-78,
Organism=Escherichia coli, GI1788024, Length=401, Percent_Identity=20.9476309226933, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI1789473, Length=323, Percent_Identity=24.1486068111455, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17537407, Length=391, Percent_Identity=27.3657289002558, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI71996953, Length=456, Percent_Identity=24.5614035087719, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI71983099, Length=450, Percent_Identity=23.7777777777778, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71983106, Length=450, Percent_Identity=23.7777777777778, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI193206505, Length=456, Percent_Identity=24.1228070175439, Blast_Score=107, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI193206654, Length=413, Percent_Identity=21.3075060532688, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17541374, Length=432, Percent_Identity=21.9907407407407, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24583025, Length=410, Percent_Identity=21.9512195121951, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI17137668, Length=410, Percent_Identity=21.9512195121951, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24583023, Length=410, Percent_Identity=21.9512195121951, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI281360481, Length=428, Percent_Identity=23.8317757009346, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI281360483, Length=428, Percent_Identity=23.8317757009346, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17137666, Length=428, Percent_Identity=23.8317757009346, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DCTA_BURP8 (B2JC54)

Other databases:

- EMBL:   CP001043
- RefSeq:   YP_001856464.1
- GeneID:   6241727
- GenomeReviews:   CP001043_GR
- KEGG:   bph:Bphy_0225
- HOGENOM:   HBG739804
- OMA:   ALATDMK
- ProtClustDB:   PRK01663
- HAMAP:   MF_01300
- InterPro:   IPR001991
- InterPro:   IPR018107
- PANTHER:   PTHR11958
- PRINTS:   PR00173

Pfam domain/function: PF00375 SDF

EC number: NA

Molecular weight: Translated: 45186; Mature: 45186

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x189313b0)-; HASH(0x1a8e4b6c)-; HASH(0x1bf25ce0)-; HASH(0x1bf5c818)-; HASH(0x1b7cb1c4)-; HASH(0x1b506df8)-; HASH(0x1bf5a248)-; HASH(0x1bf5cad0)-;

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKPIYKVLYVQVIVAIIIGIALGHFYPALATDMKPLGDAFIKLIKMVIGPIIFCTVVTG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IAGMEDMKKVGRVGGKALLYFEIVSTFALILGLAATHLLRPGVGFNVDPATLNGKEVASY
HCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
AAKAHGQTTVDFLMHIIPDTLVSAFSQGEILQILLIALLFGAVLAHVGERGKPVTNFIES
HHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
LSSILFGMVGIITKLAPIGAFGAMAFTIGKYGIGSLLPMLKLIGTFYLTSIVFVVVVLGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAKMVGFNILRFVAYIKEEMLIVLGTSSSEAALPQLMLKLEKLGCSRSVVGLVVPTGYSF
HHHHHHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCEEE
NLDGTNIYMTMAVLFIAQATNTDLTWGQQLTLLAVTMLTSKGASGVTGAGFITLAATLAV
CCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
VPTIPLSGMVLILGIDRFMSECRALTNIVGNGVATVVVSAWEKELDRSKLRAALSRDVSV
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TESAEV
CCCCCH
>Mature Secondary Structure
MKKPIYKVLYVQVIVAIIIGIALGHFYPALATDMKPLGDAFIKLIKMVIGPIIFCTVVTG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IAGMEDMKKVGRVGGKALLYFEIVSTFALILGLAATHLLRPGVGFNVDPATLNGKEVASY
HCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
AAKAHGQTTVDFLMHIIPDTLVSAFSQGEILQILLIALLFGAVLAHVGERGKPVTNFIES
HHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
LSSILFGMVGIITKLAPIGAFGAMAFTIGKYGIGSLLPMLKLIGTFYLTSIVFVVVVLGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAKMVGFNILRFVAYIKEEMLIVLGTSSSEAALPQLMLKLEKLGCSRSVVGLVVPTGYSF
HHHHHHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCEEE
NLDGTNIYMTMAVLFIAQATNTDLTWGQQLTLLAVTMLTSKGASGVTGAGFITLAATLAV
CCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
VPTIPLSGMVLILGIDRFMSECRALTNIVGNGVATVVVSAWEKELDRSKLRAALSRDVSV
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TESAEV
CCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA