Click here to switch to the map view.

The map label for this gene is lhgO [H]

Identifier: 186474876

GI number: 186474876

Start: 115417

End: 116523

Strand: Direct

Name: lhgO [H]

Synonym: Bphy_0106

Alternate gene names: 186474876

Gene position: 115417-116523 (Clockwise)

Preceding gene: 186474875

Following gene: 186474877

Centisome position: 3.32

GC content: 66.49

Gene sequence:

>1107_bases
ATGGATCAGATCGAATGTGTGGTGATCGGCGCGGGTGTCGTCGGTCTGGCCGTCGCGCGCGCTCTGGCGGCGCGCGGGCG
TGAAGTGATCGTGCTGGAAGCAGCGCAAGCCATCGGCGTCGGCACGAGTTCCCGTAACAGCGAGGTCATACACGCGGGCA
TCTACTATCCGCGCGGCTCGCTCAAGGCGACGCTGTGCGTGCGCGGCCGCGAAATGCTGTACGACTACTGCGCCGAACGC
GGCGTGCCGCATCAGCGTTGCGGCAAGCTGCTGGTCGCAACCGCACGCAATCAGGTGCCTCAGCTCGAGAGCCTGATGGC
GCGCGGCAAGGAAAACGGGGTGCTCGACTTGATGCGCATTAGCGGCGAAGAAGCCCAGGCGCTCGAACCGGCGCTGCACT
GTGTCGAAGCCGTGTTTTCGCCGCAAACGGGTATCGTCGACAGTCATCAGCTGATGCTGGCGCTGCTCGGCGACGCGGAA
CGCGATGGCGCCGTCTGCGCGTTCCACGCGCCCGTAGAAGCAATCGAGGCGGTCAACGGCCGCTTTGTGGTCAAGGTCGG
CGGCAGTTCGCCGACGACAATTGGCGCCGCGTGCGTGATCAACAGCGCCGGGCTGCATGCGAACGCGATCGCGCGCAAGA
TTCGCGGACTCGACGCCCGCCATGTGCCGCCGCTCTACCTCGCGCGCGGCAACTACTTCAGCATCTCGGGCCGTGCGCCG
TTCAACCGCCTGATCTATCCGATGCCGAATGAAGCCGGGCTCGGTGTGCATCTGACAATCGATCTCGGCGGACAGGCGCG
GTTCGGCCCGGACGTCGAATGGGTCGATTCGATCAACTACGACGTCGATCCGCAGCGTGCCGAGGCCTTCGAGGCGGCGA
TCCGCGCGTACTGGCCCGCGTTGCCAGCTCACGCGCTAAAGCCGGCGTATGCCGGTATTCGTCCCAAGCTGTCGGGGCCG
GGCGAGCCCGCCGCCGACTTCCTGATACAAGGGCCCGCCGCGCATGGCGTGCGGGGTCTCGTGAATCTGTTCGGCATCGA
GTCGCCGGGTTTGACGGCGTCGCTGGCGATTGCGCAGCGCGTGTGCGAAGTGAGCGGGCGCGCCTGA

Upstream 100 bases:

>100_bases
TTCGACGGCGCGCGGTCACGGGTCAAACGGCGCCGCACGCTATTGGAATCCGGGTGGATGACGAGGTAGAACGCGGTGCA
TCGGGCCGCGTCCAAGGGAC

Downstream 100 bases:

>100_bases
TTGCGCGGCCTGCGGCGGTGACGCTGCGTCGGCCATTGCCGGTGTGTCGTCGGTGATGCCGCGCGTGTTTCGCCCGCACA
CGTCTACCGATTTTCTTATG

Product: 2-hydroxyglutarate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MDQIECVVIGAGVVGLAVARALAARGREVIVLEAAQAIGVGTSSRNSEVIHAGIYYPRGSLKATLCVRGREMLYDYCAER
GVPHQRCGKLLVATARNQVPQLESLMARGKENGVLDLMRISGEEAQALEPALHCVEAVFSPQTGIVDSHQLMLALLGDAE
RDGAVCAFHAPVEAIEAVNGRFVVKVGGSSPTTIGAACVINSAGLHANAIARKIRGLDARHVPPLYLARGNYFSISGRAP
FNRLIYPMPNEAGLGVHLTIDLGGQARFGPDVEWVDSINYDVDPQRAEAFEAAIRAYWPALPAHALKPAYAGIRPKLSGP
GEPAADFLIQGPAAHGVRGLVNLFGIESPGLTASLAIAQRVCEVSGRA

Sequences:

>Translated_368_residues
MDQIECVVIGAGVVGLAVARALAARGREVIVLEAAQAIGVGTSSRNSEVIHAGIYYPRGSLKATLCVRGREMLYDYCAER
GVPHQRCGKLLVATARNQVPQLESLMARGKENGVLDLMRISGEEAQALEPALHCVEAVFSPQTGIVDSHQLMLALLGDAE
RDGAVCAFHAPVEAIEAVNGRFVVKVGGSSPTTIGAACVINSAGLHANAIARKIRGLDARHVPPLYLARGNYFSISGRAP
FNRLIYPMPNEAGLGVHLTIDLGGQARFGPDVEWVDSINYDVDPQRAEAFEAAIRAYWPALPAHALKPAYAGIRPKLSGP
GEPAADFLIQGPAAHGVRGLVNLFGIESPGLTASLAIAQRVCEVSGRA
>Mature_368_residues
MDQIECVVIGAGVVGLAVARALAARGREVIVLEAAQAIGVGTSSRNSEVIHAGIYYPRGSLKATLCVRGREMLYDYCAER
GVPHQRCGKLLVATARNQVPQLESLMARGKENGVLDLMRISGEEAQALEPALHCVEAVFSPQTGIVDSHQLMLALLGDAE
RDGAVCAFHAPVEAIEAVNGRFVVKVGGSSPTTIGAACVINSAGLHANAIARKIRGLDARHVPPLYLARGNYFSISGRAP
FNRLIYPMPNEAGLGVHLTIDLGGQARFGPDVEWVDSINYDVDPQRAEAFEAAIRAYWPALPAHALKPAYAGIRPKLSGP
GEPAADFLIQGPAAHGVRGLVNLFGIESPGLTASLAIAQRVCEVSGRA

Specific function: Oxidizes L-2-hydroxyglutarate, probably to recover alpha-ketoglutarate mistakenly reduced by other enzymes or formed during growth on propionate [H]

COG id: COG0579

COG function: function code R; Predicted dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the L2HGDH family [H]

Homologues:

Organism=Homo sapiens, GI13376331, Length=273, Percent_Identity=33.6996336996337, Blast_Score=136, Evalue=4e-32,
Organism=Escherichia coli, GI87082148, Length=249, Percent_Identity=30.9236947791165, Blast_Score=104, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17565764, Length=407, Percent_Identity=28.2555282555283, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24585081, Length=400, Percent_Identity=30.75, Blast_Score=128, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015657
- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.1.3.15 [H]

Molecular weight: Translated: 38830; Mature: 38830

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQIECVVIGAGVVGLAVARALAARGREVIVLEAAQAIGVGTSSRNSEVIHAGIYYPRGS
CCCEEEEEEECCHHHHHHHHHHHHCCCEEEEEEECHHHCCCCCCCCCCEEEEEEECCCCC
LKATLCVRGREMLYDYCAERGVPHQRCGKLLVATARNQVPQLESLMARGKENGVLDLMRI
CEEEEEECCHHHHHHHHHHCCCCHHHHCCEEEEHHHCCCHHHHHHHHCCCCCCCEEEEEE
SGEEAQALEPALHCVEAVFSPQTGIVDSHQLMLALLGDAERDGAVCAFHAPVEAIEAVNG
CCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCHHHHHHCCC
RFVVKVGGSSPTTIGAACVINSAGLHANAIARKIRGLDARHVPPLYLARGNYFSISGRAP
EEEEEECCCCCCCEEEEEEEECCCCCHHHHHHHHHCCCCCCCCCEEEECCCEEEEECCCC
FNRLIYPMPNEAGLGVHLTIDLGGQARFGPDVEWVDSINYDVDPQRAEAFEAAIRAYWPA
HHHCEEECCCCCCCEEEEEEEECCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHCC
LPAHALKPAYAGIRPKLSGPGEPAADFLIQGPAAHGVRGLVNLFGIESPGLTASLAIAQR
CCHHHCCCHHCCCCCCCCCCCCCHHHHEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHH
VCEVSGRA
HHHHCCCC
>Mature Secondary Structure
MDQIECVVIGAGVVGLAVARALAARGREVIVLEAAQAIGVGTSSRNSEVIHAGIYYPRGS
CCCEEEEEEECCHHHHHHHHHHHHCCCEEEEEEECHHHCCCCCCCCCCEEEEEEECCCCC
LKATLCVRGREMLYDYCAERGVPHQRCGKLLVATARNQVPQLESLMARGKENGVLDLMRI
CEEEEEECCHHHHHHHHHHCCCCHHHHCCEEEEHHHCCCHHHHHHHHCCCCCCCEEEEEE
SGEEAQALEPALHCVEAVFSPQTGIVDSHQLMLALLGDAERDGAVCAFHAPVEAIEAVNG
CCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCHHHHHHCCC
RFVVKVGGSSPTTIGAACVINSAGLHANAIARKIRGLDARHVPPLYLARGNYFSISGRAP
EEEEEECCCCCCCEEEEEEEECCCCCHHHHHHHHHCCCCCCCCCEEEECCCEEEEECCCC
FNRLIYPMPNEAGLGVHLTIDLGGQARFGPDVEWVDSINYDVDPQRAEAFEAAIRAYWPA
HHHCEEECCCCCCCEEEEEEEECCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHCC
LPAHALKPAYAGIRPKLSGPGEPAADFLIQGPAAHGVRGLVNLFGIESPGLTASLAIAQR
CCHHHCCCHHCCCCCCCCCCCCCHHHHEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHH
VCEVSGRA
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 8297211; 7984428 [H]