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The map label for this gene is gidA

Identifier: 183222816

GI number: 183222816

Start: 3595631

End: 3597505

Strand: Reverse

Name: gidA

Synonym: LEPBI_I3477

Alternate gene names: 183222816

Gene position: 3597505-3595631 (Counterclockwise)

Preceding gene: NA

Following gene: 183222814

Centisome position: 99.94

GC content: 41.17

Gene sequence:

>1875_bases
ATGAATCCTTCCTTTTATCCAAACCAATTTGATTGTATCGTTGTGGGTGCCGGTCATGCCGGAACAGAAGCCGCTTATAT
CTCTGCAAAAGCAGGTCTGAAAACTTTACTCATAACGATGAACTTAGATACCATCGGTCAAATGAGTTGTAATCCAGCCA
TCGGTGGGATTGCAAAAGGCCATATGGTTCGGGAAGTGGATGCTCTTGGTGGTCTTATGGGACGAGTGATCGACCAAACT
GGAATCCAGTTTAAGATGTTAAACACTTCCAAAGGACCTTCTGTTTGGGCCCCACGTGCGCAGGCAGAGAAAAAACAATA
CCAACTCATGATCAAACACCAGTTGGAAAAATTACAACAGCTTTCGATCCGACAAGATACTGTTGAAGATTTGATTGTGG
AAGGAAACCAAGTCACAGGTGTCATCACCGGCCGTGGTTTTACTTTTTATACAAACCATGTGATCTTAACCACAGGAACA
TTTTTATCCAGTGTGATCCATATAGGAACATACCAAAAAGAATCAGGTCGGATTGGAGAACCAACAACGAAAGGTCTTTC
CCATACTCTTGCTCGTTTTGAATTACGCTTAGGTCGTTTGAAAACAGGAACCCCTGCGCGTGTTCATAAAAATTCGATCA
ACTTTGATGGTCTCGAAATCCAAGATGGAGATGAAAATCCAAGACCCTTTTCTTTTTCCACAAAGAAGATCGATCGCAAA
CAAATCCCTTGTTTCATCACCTACACGAATGATACAACTCATGAACTGATCAAACAAAACTTAGAATACTCACCGATGTA
TTCGGGTCAGATCAAAAGTGTGGGACCAAGATACTGTCCTTCGATCGAAGACAAAGTGGTTCGTTTTGCAGAACGAGACA
GACACCAAATTTTCATCGAACCAGAAGGTTATGAAACAAATGAGATGTATCTCAATGGTGTCTCCACAAGTTTACCGGAA
GAAGTGCAATGGAAGTTCCTTCGAAGCATCAAAGGTTTAGAGCAAGTGGAACTCATGCGACCTGGTTATGCCATCGAATA
TGATTATGTTGATCCAACAGAACTCCATCCGACTCTCGAAACAAAAAAGGTAAAAGGACTCTACCATGCAGGCCAAATCA
ATGGGACCACAGGGTATGAAGAAGCAGCGGCTCAAGGACTTGTCGCTGCGTATAATGTGATACGTTCCGTTCGGAAAGAA
GAACCTATTTTATTCAAACGAAGTGAATCCTATATTGGAGTGCTTGTGGATGATCTCGTCTACAAAGGTGTGGAAGATCC
GTATCGAATGTTTACAAGTCGTGCGGAATATCGCCTTCTTCTCAGACAAGACAACGCTGACCAAAGGCTCATGCAATACG
GATATGAGATGGGTCTTGTGGAAGAATCATTATACAAAGATATGAAAGATCGTTATGCCAGGATTGAGTCGATTAAATCC
CATTTGTTTGTCTCTGCGATGAAACCGTCTCCAGAGCTCACAAAAGTTTTGGAAGAGAAACAGATTACGAATTATAAATT
TGGTCACTCGCTTTCCTCTTTTCTCAAACGATCAGACATCAAAATTAAAGATTTAGAACCAATCGTTTCCGAACTTTCGA
TTTTGAATGAGGATGAAAAAGCAGTTTTGGAAATGGAAGTGAAGTATGAAGGGTATCTGAAACGAGAACTAGAGACCATC
GAATACCGTAAGAAGTTTTTAAACTTTCAGATTCCCATTGATTTTGATTACGCGAGTGTGAAAGGTTTGAAGACGGAAGC
CATCGTGAAATTAGAAAAACATAGACCACTCAATTTAGAAAATGCCCTTCATATTTCGGGAGTGGATCCTTCCGATGTGG
ATTTACTTCTCTATCATTTGGTGGATCGACATTGA

Upstream 100 bases:

>100_bases
GAAGCATAGGCCGATGGTTTTGATATCGTTCTAGAGAGTAGTCTTTACTCTCCTTTATTTTTCATAAGACATAAGAATCG
TTACACTAAGAAGACATAAT

Downstream 100 bases:

>100_bases
TCAAATAATTTTATTCCACTCGCAGATTCATTACCGACGATATGAAAAAAACCAACCAAGAGAGATTCCTTTTGGTTGGT
TTTTTTATGGGAACCTTTAT

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A

Number of amino acids: Translated: 624; Mature: 624

Protein sequence:

>624_residues
MNPSFYPNQFDCIVVGAGHAGTEAAYISAKAGLKTLLITMNLDTIGQMSCNPAIGGIAKGHMVREVDALGGLMGRVIDQT
GIQFKMLNTSKGPSVWAPRAQAEKKQYQLMIKHQLEKLQQLSIRQDTVEDLIVEGNQVTGVITGRGFTFYTNHVILTTGT
FLSSVIHIGTYQKESGRIGEPTTKGLSHTLARFELRLGRLKTGTPARVHKNSINFDGLEIQDGDENPRPFSFSTKKIDRK
QIPCFITYTNDTTHELIKQNLEYSPMYSGQIKSVGPRYCPSIEDKVVRFAERDRHQIFIEPEGYETNEMYLNGVSTSLPE
EVQWKFLRSIKGLEQVELMRPGYAIEYDYVDPTELHPTLETKKVKGLYHAGQINGTTGYEEAAAQGLVAAYNVIRSVRKE
EPILFKRSESYIGVLVDDLVYKGVEDPYRMFTSRAEYRLLLRQDNADQRLMQYGYEMGLVEESLYKDMKDRYARIESIKS
HLFVSAMKPSPELTKVLEEKQITNYKFGHSLSSFLKRSDIKIKDLEPIVSELSILNEDEKAVLEMEVKYEGYLKRELETI
EYRKKFLNFQIPIDFDYASVKGLKTEAIVKLEKHRPLNLENALHISGVDPSDVDLLLYHLVDRH

Sequences:

>Translated_624_residues
MNPSFYPNQFDCIVVGAGHAGTEAAYISAKAGLKTLLITMNLDTIGQMSCNPAIGGIAKGHMVREVDALGGLMGRVIDQT
GIQFKMLNTSKGPSVWAPRAQAEKKQYQLMIKHQLEKLQQLSIRQDTVEDLIVEGNQVTGVITGRGFTFYTNHVILTTGT
FLSSVIHIGTYQKESGRIGEPTTKGLSHTLARFELRLGRLKTGTPARVHKNSINFDGLEIQDGDENPRPFSFSTKKIDRK
QIPCFITYTNDTTHELIKQNLEYSPMYSGQIKSVGPRYCPSIEDKVVRFAERDRHQIFIEPEGYETNEMYLNGVSTSLPE
EVQWKFLRSIKGLEQVELMRPGYAIEYDYVDPTELHPTLETKKVKGLYHAGQINGTTGYEEAAAQGLVAAYNVIRSVRKE
EPILFKRSESYIGVLVDDLVYKGVEDPYRMFTSRAEYRLLLRQDNADQRLMQYGYEMGLVEESLYKDMKDRYARIESIKS
HLFVSAMKPSPELTKVLEEKQITNYKFGHSLSSFLKRSDIKIKDLEPIVSELSILNEDEKAVLEMEVKYEGYLKRELETI
EYRKKFLNFQIPIDFDYASVKGLKTEAIVKLEKHRPLNLENALHISGVDPSDVDLLLYHLVDRH
>Mature_624_residues
MNPSFYPNQFDCIVVGAGHAGTEAAYISAKAGLKTLLITMNLDTIGQMSCNPAIGGIAKGHMVREVDALGGLMGRVIDQT
GIQFKMLNTSKGPSVWAPRAQAEKKQYQLMIKHQLEKLQQLSIRQDTVEDLIVEGNQVTGVITGRGFTFYTNHVILTTGT
FLSSVIHIGTYQKESGRIGEPTTKGLSHTLARFELRLGRLKTGTPARVHKNSINFDGLEIQDGDENPRPFSFSTKKIDRK
QIPCFITYTNDTTHELIKQNLEYSPMYSGQIKSVGPRYCPSIEDKVVRFAERDRHQIFIEPEGYETNEMYLNGVSTSLPE
EVQWKFLRSIKGLEQVELMRPGYAIEYDYVDPTELHPTLETKKVKGLYHAGQINGTTGYEEAAAQGLVAAYNVIRSVRKE
EPILFKRSESYIGVLVDDLVYKGVEDPYRMFTSRAEYRLLLRQDNADQRLMQYGYEMGLVEESLYKDMKDRYARIESIKS
HLFVSAMKPSPELTKVLEEKQITNYKFGHSLSSFLKRSDIKIKDLEPIVSELSILNEDEKAVLEMEVKYEGYLKRELETI
EYRKKFLNFQIPIDFDYASVKGLKTEAIVKLEKHRPLNLENALHISGVDPSDVDLLLYHLVDRH

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family

Homologues:

Organism=Homo sapiens, GI74024895, Length=635, Percent_Identity=41.8897637795276, Blast_Score=452, Evalue=1e-127,
Organism=Homo sapiens, GI19882217, Length=660, Percent_Identity=40.3030303030303, Blast_Score=438, Evalue=1e-123,
Organism=Homo sapiens, GI183227703, Length=675, Percent_Identity=39.4074074074074, Blast_Score=433, Evalue=1e-121,
Organism=Escherichia coli, GI2367273, Length=623, Percent_Identity=50.0802568218299, Blast_Score=611, Evalue=1e-176,
Organism=Caenorhabditis elegans, GI17534255, Length=627, Percent_Identity=39.2344497607655, Blast_Score=448, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6321202, Length=624, Percent_Identity=42.9487179487179, Blast_Score=487, Evalue=1e-138,
Organism=Drosophila melanogaster, GI24658174, Length=631, Percent_Identity=40.5705229793978, Blast_Score=459, Evalue=1e-129,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMG_LEPBA (B0S904)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001961126.1
- ProteinModelPortal:   B0S904
- SMR:   B0S904
- GeneID:   6386943
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_0001
- HOGENOM:   HBG284774
- OMA:   GIQFRVL
- ProtClustDB:   PRK05192
- BioCyc:   LBIF355278:LBF_0001-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00129
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00136

Pfam domain/function: PF01134 GIDA

EC number: NA

Molecular weight: Translated: 70984; Mature: 70984

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: BINDING 128-128 BINDING 183-183 BINDING 372-372

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPSFYPNQFDCIVVGAGHAGTEAAYISAKAGLKTLLITMNLDTIGQMSCNPAIGGIAKG
CCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCCC
HMVREVDALGGLMGRVIDQTGIQFKMLNTSKGPSVWAPRAQAEKKQYQLMIKHQLEKLQQ
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LSIRQDTVEDLIVEGNQVTGVITGRGFTFYTNHVILTTGTFLSSVIHIGTYQKESGRIGE
HHHHHHHHHHHHHCCCEEEEEEECCCEEEEECEEEEEECHHHHHHHHHCCCCCCCCCCCC
PTTKGLSHTLARFELRLGRLKTGTPARVHKNSINFDGLEIQDGDENPRPFSFSTKKIDRK
CHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCCEEEECCCCCCCCCCCCCCCCHHC
QIPCFITYTNDTTHELIKQNLEYSPMYSGQIKSVGPRYCPSIEDKVVRFAERDRHQIFIE
CCCEEEEECCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEC
PEGYETNEMYLNGVSTSLPEEVQWKFLRSIKGLEQVELMRPGYAIEYDYVDPTELHPTLE
CCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCEEEECCCCCCCCCCCCH
TKKVKGLYHAGQINGTTGYEEAAAQGLVAAYNVIRSVRKEEPILFKRSESYIGVLVDDLV
HHHHCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHH
YKGVEDPYRMFTSRAEYRLLLRQDNADQRLMQYGYEMGLVEESLYKDMKDRYARIESIKS
HCCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHCHHCCHHHHHHHHHHHHHHHHHHHHHH
HLFVSAMKPSPELTKVLEEKQITNYKFGHSLSSFLKRSDIKIKDLEPIVSELSILNEDEK
HHEEEECCCCHHHHHHHHHHHHCCEECHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCC
AVLEMEVKYEGYLKRELETIEYRKKFLNFQIPIDFDYASVKGLKTEAIVKLEKHRPLNLE
EEEEEEECHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHCCCCCHHHHEEECCCCCCCCC
NALHISGVDPSDVDLLLYHLVDRH
CEEEECCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MNPSFYPNQFDCIVVGAGHAGTEAAYISAKAGLKTLLITMNLDTIGQMSCNPAIGGIAKG
CCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCCC
HMVREVDALGGLMGRVIDQTGIQFKMLNTSKGPSVWAPRAQAEKKQYQLMIKHQLEKLQQ
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LSIRQDTVEDLIVEGNQVTGVITGRGFTFYTNHVILTTGTFLSSVIHIGTYQKESGRIGE
HHHHHHHHHHHHHCCCEEEEEEECCCEEEEECEEEEEECHHHHHHHHHCCCCCCCCCCCC
PTTKGLSHTLARFELRLGRLKTGTPARVHKNSINFDGLEIQDGDENPRPFSFSTKKIDRK
CHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCCEEEECCCCCCCCCCCCCCCCHHC
QIPCFITYTNDTTHELIKQNLEYSPMYSGQIKSVGPRYCPSIEDKVVRFAERDRHQIFIE
CCCEEEEECCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEC
PEGYETNEMYLNGVSTSLPEEVQWKFLRSIKGLEQVELMRPGYAIEYDYVDPTELHPTLE
CCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCEEEECCCCCCCCCCCCH
TKKVKGLYHAGQINGTTGYEEAAAQGLVAAYNVIRSVRKEEPILFKRSESYIGVLVDDLV
HHHHCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHH
YKGVEDPYRMFTSRAEYRLLLRQDNADQRLMQYGYEMGLVEESLYKDMKDRYARIESIKS
HCCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHCHHCCHHHHHHHHHHHHHHHHHHHHHH
HLFVSAMKPSPELTKVLEEKQITNYKFGHSLSSFLKRSDIKIKDLEPIVSELSILNEDEK
HHEEEECCCCHHHHHHHHHHHHCCEECHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCC
AVLEMEVKYEGYLKRELETIEYRKKFLNFQIPIDFDYASVKGLKTEAIVKLEKHRPLNLE
EEEEEEECHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHCCCCCHHHHEEECCCCCCCCC
NALHISGVDPSDVDLLLYHLVDRH
CEEEECCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA