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The map label for this gene is nuoI

Identifier: 183222747

GI number: 183222747

Start: 3525465

End: 3525992

Strand: Reverse

Name: nuoI

Synonym: LEPBI_I3405

Alternate gene names: 183222747

Gene position: 3525992-3525465 (Counterclockwise)

Preceding gene: 183222748

Following gene: 183222746

Centisome position: 97.95

GC content: 41.67

Gene sequence:

>528_bases
TTGGGAACCGTTAATGTCGTCAACGTAGCCAAAAAACACCAGTTTTCTTGGTATGAAAAATTTTACTTTTGGTCCATTGG
CAAAGGCCTTTGGATCACCCTCAAACATTTTGTGAAGGTTGCGTTCTTTAACAAACAAGTCACAATTGAATACCCTGATA
AAAAAAGACAATACTCAACTCGCTTTCGTGGAATGCACTCTATGAAACGAGATGAACAAGGTCGTGAGAGGTGTACGGCA
TGTTTTTGTTGTATGTGGATTTGTCCTGCCAATGCGATTCATATCGAAGCGGCAGAAGTCACTGCTGAACGCCAACACCT
TCACCCCGAAGACAAATATGCCAAAAAATTTGAAATCAATTTACTTCGCTGCATCTTCTGTGGGTTATGCGAAGAAGCAT
GTCCCAAAGGAGCTATTTATTTGGATGGAACCGGAGAGATGGCAGCTGACAACAGGGAAGATTTGTTTTTGACCAAAGAA
AGAATGATGGAAAAAACAGGTGGTCCCATCCTCGGCCAAAGGAATTAA

Upstream 100 bases:

>100_bases
ACGTTTGAAAAGTCAATGAATCCCATCCAAGGATTACAAACTTCCGCAGGTGTTGTGGAACTCATTGTGAAAACCATGAC
AAAAAAAATGGAGGCATCCG

Downstream 100 bases:

>100_bases
GATTCTAACTTACCTTCGAAACATAGACCCGGAATCGTATTTTCAAAATGCATCCGGGTTTCTTACCTAACCATTTCATT
CCAGTATTTGTGTTTTTCAA

Product: NADH-quinone oxidoreductase chain I

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I; NDH-1 subunit I

Number of amino acids: Translated: 175; Mature: 174

Protein sequence:

>175_residues
MGTVNVVNVAKKHQFSWYEKFYFWSIGKGLWITLKHFVKVAFFNKQVTIEYPDKKRQYSTRFRGMHSMKRDEQGRERCTA
CFCCMWICPANAIHIEAAEVTAERQHLHPEDKYAKKFEINLLRCIFCGLCEEACPKGAIYLDGTGEMAADNREDLFLTKE
RMMEKTGGPILGQRN

Sequences:

>Translated_175_residues
MGTVNVVNVAKKHQFSWYEKFYFWSIGKGLWITLKHFVKVAFFNKQVTIEYPDKKRQYSTRFRGMHSMKRDEQGRERCTA
CFCCMWICPANAIHIEAAEVTAERQHLHPEDKYAKKFEINLLRCIFCGLCEEACPKGAIYLDGTGEMAADNREDLFLTKE
RMMEKTGGPILGQRN
>Mature_174_residues
GTVNVVNVAKKHQFSWYEKFYFWSIGKGLWITLKHFVKVAFFNKQVTIEYPDKKRQYSTRFRGMHSMKRDEQGRERCTAC
FCCMWICPANAIHIEAAEVTAERQHLHPEDKYAKKFEINLLRCIFCGLCEEACPKGAIYLDGTGEMAADNREDLFLTKER
MMEKTGGPILGQRN

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Homo sapiens, GI4505371, Length=147, Percent_Identity=37.4149659863946, Blast_Score=96, Evalue=1e-20,
Organism=Escherichia coli, GI1788617, Length=138, Percent_Identity=37.6811594202899, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1789075, Length=125, Percent_Identity=34.4, Blast_Score=65, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17555194, Length=143, Percent_Identity=36.3636363636364, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI17864306, Length=143, Percent_Identity=36.3636363636364, Blast_Score=100, Evalue=4e-22,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOI_LEPBA (B0SHV5)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001964334.1
- ProteinModelPortal:   B0SHV5
- SMR:   B0SHV5
- GeneID:   6389064
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_3289
- HOGENOM:   HBG724359
- OMA:   VEGGDNT
- ProtClustDB:   CLSK814127
- BioCyc:   LBIF355278:LBF_3289-MONOMER
- HAMAP:   MF_01351
- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226
- Gene3D:   G3DSA:1.10.1060.10
- TIGRFAMs:   TIGR01971

Pfam domain/function: PF00037 Fer4

EC number: =1.6.99.5

Molecular weight: Translated: 20403; Mature: 20272

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.1 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
9.1 %Cys+Met (Translated Protein)
5.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
8.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTVNVVNVAKKHQFSWYEKFYFWSIGKGLWITLKHFVKVAFFNKQVTIEYPDKKRQYST
CCCCHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCHHHHHHH
RFRGMHSMKRDEQGRERCTACFCCMWICPANAIHIEAAEVTAERQHLHPEDKYAKKFEIN
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHCCCCHHHHHHHHHH
LLRCIFCGLCEEACPKGAIYLDGTGEMAADNREDLFLTKERMMEKTGGPILGQRN
HHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCEEEEHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
GTVNVVNVAKKHQFSWYEKFYFWSIGKGLWITLKHFVKVAFFNKQVTIEYPDKKRQYST
CCCHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCHHHHHHH
RFRGMHSMKRDEQGRERCTACFCCMWICPANAIHIEAAEVTAERQHLHPEDKYAKKFEIN
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHCCCCHHHHHHHHHH
LLRCIFCGLCEEACPKGAIYLDGTGEMAADNREDLFLTKERMMEKTGGPILGQRN
HHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCEEEEHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA