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The map label for this gene is ilvD [H]

Identifier: 183222696

GI number: 183222696

Start: 3461420

End: 3463096

Strand: Reverse

Name: ilvD [H]

Synonym: LEPBI_I3352

Alternate gene names: 183222696

Gene position: 3463096-3461420 (Counterclockwise)

Preceding gene: 183222697

Following gene: 183222692

Centisome position: 96.21

GC content: 42.1

Gene sequence:

>1677_bases
ATGAGTTTGAATCGATACAGCCGTGTTTTAACCCAAGACGAATCTCTCCCTGCATCCCAAGCAATGATCATTGGTTCAGG
AGTTCCTTACGAAGATTTGAACAAACCTTTTGTCGGAATAGGTAGTACAGGTTTTGACGGAAATCCTTGTAACATGCATT
TGACAACCCTTGCTGCTTTACAAAAGAAAAGTGTCCTTGATACAAAACAAATGGTTGGACTTTTGTTTAACACCATCGGT
GTGAGCGATGGAATCACGAATGGAAATGATGGAATGCGTTATTCCCTTCCGTCACGTGAGATCATTGCTGATTCGATTGA
AACGATCTCAGGTGCTCATTTTTATGACGGAATTATTTTCACTGCTGGATGTGATAAAAATATGCCAGGTGCTATTATGG
CGATGGCAAGACTCAACCGTCCCGCCATCATGGTGTATGGCGGAACCATCAATGGTGGCCACTTCAAAGGAGAAAAATTA
AATATTGTTTCTGCCTTCGAAGCATATGGAAAAAAAATTAACGGAAAAATTTCAGAAGAAGATTTCAAAGAAGTGATCAA
AAACTCCTGCCCAGGTCCTGGTGCGTGCGGTGGAATGTACACTGCAAATACAATGGCAACAGCGATCGAAGTGATGGGGA
TGAGTTTGCCATATAGTTCTTCCTCACCGGCACGCAGTGAAGAAAAAAAGAAAGAATGCCAAGAAATTGGAAAGTACATG
TACAATCTTTTAGAAAAAGACATCAAACCTTCTGATATCATCACTCCTAAATCCATCTTAAATGCACTCCGTGTCATCAC
AATCCTTGGTGGATCAACGAATGCAGCACTCCATATGATAGCCATCGCAAGGACAATGGGAATTGATTTAGACCTCGATC
AAATCCAAAAGGTAACGGATACAACACCATTACTTGCCGACATGAAACCAAGTGGAAAGTATTTGATGGAAGATCTTTTT
GCCATTGGTGGAACACCTGCCATCATGAAATTTATGTTAAATGAAGGTATGTTAGATGGTTCCTGTTTAACTGTCACTGG
AAAAACCATCGCAGAGAATTTAGAATCTCTACCGGACCTTCCCAAAGACCAAGACTTACTTAGACCTGTCAGTAACCCGA
TCAAAAAAGAAGGTCATATACAAGTGTTATATGGTAATATCGCCAAAAAAGGTGCAGTGGCAAAAATCACTGGGCATGAA
GGCGAAATGTTTGAAGGCAAAGCCATTTGTTTTGATTCTGAAGTCGCAGCAAACGAAGGGATTCGGGATGGAAAAGTGAA
ACCTGGCCATGTTGTTGTCATTCGTTATGTGGGTCCAAAAGGGGGACCAGGGATGCCAGAGATGTTAAAACCAACTTCTG
CCATCATTGGAGCTGGCCTTGGTGACAATGTTGCTCTCATCACTGATGGAAGGTTCTCTGGTGGAAGCCATGGATTTGTA
GTGGGTCATATCACTCCAGAAGCCATGGAAGGTGGAGAAATTGCTCTCGTTGAAGATGGGGATGTAATTTCGATCAATGC
ACGCACAAATACACTCGATGTCAAAGTCAGCGCTGAAGATCTGGAGAAACGACGTAAAAAATGGAAAAAACCTCCTTACC
GAGTGACAAGCGGTTACCTATGGAAGTACATCCAAATGGTAAAAGATGCAAGTACAGGTTGTTTGACAGACCGGTAA

Upstream 100 bases:

>100_bases
AAAATTCTCATCGAAATCATAGTACCTTAATTTTGAAAATCCGCTTTCCTTTCTCTTCCACTTTTACATTTTGACTTTAA
TCCGAAGGAAATTGTAAATT

Downstream 100 bases:

>100_bases
TCCTACCGGTCGCATAACATTCATTACATACTTATTGAGACTGAGAGGGAAATTCCTCTTTCCCGTTCGCATGTTTGGCA
AAACGACCTTTGCCCACATG

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD [H]

Number of amino acids: Translated: 558; Mature: 557

Protein sequence:

>558_residues
MSLNRYSRVLTQDESLPASQAMIIGSGVPYEDLNKPFVGIGSTGFDGNPCNMHLTTLAALQKKSVLDTKQMVGLLFNTIG
VSDGITNGNDGMRYSLPSREIIADSIETISGAHFYDGIIFTAGCDKNMPGAIMAMARLNRPAIMVYGGTINGGHFKGEKL
NIVSAFEAYGKKINGKISEEDFKEVIKNSCPGPGACGGMYTANTMATAIEVMGMSLPYSSSSPARSEEKKKECQEIGKYM
YNLLEKDIKPSDIITPKSILNALRVITILGGSTNAALHMIAIARTMGIDLDLDQIQKVTDTTPLLADMKPSGKYLMEDLF
AIGGTPAIMKFMLNEGMLDGSCLTVTGKTIAENLESLPDLPKDQDLLRPVSNPIKKEGHIQVLYGNIAKKGAVAKITGHE
GEMFEGKAICFDSEVAANEGIRDGKVKPGHVVVIRYVGPKGGPGMPEMLKPTSAIIGAGLGDNVALITDGRFSGGSHGFV
VGHITPEAMEGGEIALVEDGDVISINARTNTLDVKVSAEDLEKRRKKWKKPPYRVTSGYLWKYIQMVKDASTGCLTDR

Sequences:

>Translated_558_residues
MSLNRYSRVLTQDESLPASQAMIIGSGVPYEDLNKPFVGIGSTGFDGNPCNMHLTTLAALQKKSVLDTKQMVGLLFNTIG
VSDGITNGNDGMRYSLPSREIIADSIETISGAHFYDGIIFTAGCDKNMPGAIMAMARLNRPAIMVYGGTINGGHFKGEKL
NIVSAFEAYGKKINGKISEEDFKEVIKNSCPGPGACGGMYTANTMATAIEVMGMSLPYSSSSPARSEEKKKECQEIGKYM
YNLLEKDIKPSDIITPKSILNALRVITILGGSTNAALHMIAIARTMGIDLDLDQIQKVTDTTPLLADMKPSGKYLMEDLF
AIGGTPAIMKFMLNEGMLDGSCLTVTGKTIAENLESLPDLPKDQDLLRPVSNPIKKEGHIQVLYGNIAKKGAVAKITGHE
GEMFEGKAICFDSEVAANEGIRDGKVKPGHVVVIRYVGPKGGPGMPEMLKPTSAIIGAGLGDNVALITDGRFSGGSHGFV
VGHITPEAMEGGEIALVEDGDVISINARTNTLDVKVSAEDLEKRRKKWKKPPYRVTSGYLWKYIQMVKDASTGCLTDR
>Mature_557_residues
SLNRYSRVLTQDESLPASQAMIIGSGVPYEDLNKPFVGIGSTGFDGNPCNMHLTTLAALQKKSVLDTKQMVGLLFNTIGV
SDGITNGNDGMRYSLPSREIIADSIETISGAHFYDGIIFTAGCDKNMPGAIMAMARLNRPAIMVYGGTINGGHFKGEKLN
IVSAFEAYGKKINGKISEEDFKEVIKNSCPGPGACGGMYTANTMATAIEVMGMSLPYSSSSPARSEEKKKECQEIGKYMY
NLLEKDIKPSDIITPKSILNALRVITILGGSTNAALHMIAIARTMGIDLDLDQIQKVTDTTPLLADMKPSGKYLMEDLFA
IGGTPAIMKFMLNEGMLDGSCLTVTGKTIAENLESLPDLPKDQDLLRPVSNPIKKEGHIQVLYGNIAKKGAVAKITGHEG
EMFEGKAICFDSEVAANEGIRDGKVKPGHVVVIRYVGPKGGPGMPEMLKPTSAIIGAGLGDNVALITDGRFSGGSHGFVV
GHITPEAMEGGEIALVEDGDVISINARTNTLDVKVSAEDLEKRRKKWKKPPYRVTSGYLWKYIQMVKDASTGCLTDR

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=608, Percent_Identity=34.2105263157895, Blast_Score=317, Evalue=1e-87,
Organism=Escherichia coli, GI1788157, Length=467, Percent_Identity=31.2633832976445, Blast_Score=196, Evalue=4e-51,
Organism=Escherichia coli, GI2367371, Length=531, Percent_Identity=29.9435028248588, Blast_Score=175, Evalue=9e-45,
Organism=Escherichia coli, GI1786464, Length=526, Percent_Identity=29.0874524714829, Blast_Score=167, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6322476, Length=563, Percent_Identity=52.0426287744227, Blast_Score=599, Evalue=1e-172,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 59874; Mature: 59743

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00886 ILVD_EDD_1 ; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLNRYSRVLTQDESLPASQAMIIGSGVPYEDLNKPFVGIGSTGFDGNPCNMHLTTLAAL
CCCHHHHHHHHCCCCCCCCCEEEEECCCCHHHCCCCEEECCCCCCCCCCCCEEHHHHHHH
QKKSVLDTKQMVGLLFNTIGVSDGITNGNDGMRYSLPSREIIADSIETISGAHFYDGIIF
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCEECCCEEE
TAGCDKNMPGAIMAMARLNRPAIMVYGGTINGGHFKGEKLNIVSAFEAYGKKINGKISEE
EECCCCCCCHHHHHHHHCCCCEEEEECCEECCCCCCCCEEEHHHHHHHHCHHCCCCCCHH
DFKEVIKNSCPGPGACGGMYTANTMATAIEVMGMSLPYSSSSPARSEEKKKECQEIGKYM
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
YNLLEKDIKPSDIITPKSILNALRVITILGGSTNAALHMIAIARTMGIDLDLDQIQKVTD
HHHHHHCCCCCCCCCHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHC
TTPLLADMKPSGKYLMEDLFAIGGTPAIMKFMLNEGMLDGSCLTVTGKTIAENLESLPDL
CCCCEECCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHCCCC
PKDQDLLRPVSNPIKKEGHIQVLYGNIAKKGAVAKITGHEGEMFEGKAICFDSEVAANEG
CCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEECCCCCEECCCEEEECCCHHHCCC
IRDGKVKPGHVVVIRYVGPKGGPGMPEMLKPTSAIIGAGLGDNVALITDGRFSGGSHGFV
CCCCCCCCCCEEEEEEECCCCCCCCHHHHCCCHHHHCCCCCCCEEEEECCCCCCCCCCEE
VGHITPEAMEGGEIALVEDGDVISINARTNTLDVKVSAEDLEKRRKKWKKPPYRVTSGYL
EEECCHHHCCCCCEEEEECCCEEEEECCCEEEEEEECHHHHHHHHHHHCCCCCCCCHHHH
WKYIQMVKDASTGCLTDR
HHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SLNRYSRVLTQDESLPASQAMIIGSGVPYEDLNKPFVGIGSTGFDGNPCNMHLTTLAAL
CCHHHHHHHHCCCCCCCCCEEEEECCCCHHHCCCCEEECCCCCCCCCCCCEEHHHHHHH
QKKSVLDTKQMVGLLFNTIGVSDGITNGNDGMRYSLPSREIIADSIETISGAHFYDGIIF
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCEECCCEEE
TAGCDKNMPGAIMAMARLNRPAIMVYGGTINGGHFKGEKLNIVSAFEAYGKKINGKISEE
EECCCCCCCHHHHHHHHCCCCEEEEECCEECCCCCCCCEEEHHHHHHHHCHHCCCCCCHH
DFKEVIKNSCPGPGACGGMYTANTMATAIEVMGMSLPYSSSSPARSEEKKKECQEIGKYM
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
YNLLEKDIKPSDIITPKSILNALRVITILGGSTNAALHMIAIARTMGIDLDLDQIQKVTD
HHHHHHCCCCCCCCCHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHC
TTPLLADMKPSGKYLMEDLFAIGGTPAIMKFMLNEGMLDGSCLTVTGKTIAENLESLPDL
CCCCEECCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHCCCC
PKDQDLLRPVSNPIKKEGHIQVLYGNIAKKGAVAKITGHEGEMFEGKAICFDSEVAANEG
CCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEECCCCCEECCCEEEECCCHHHCCC
IRDGKVKPGHVVVIRYVGPKGGPGMPEMLKPTSAIIGAGLGDNVALITDGRFSGGSHGFV
CCCCCCCCCCEEEEEEECCCCCCCCHHHHCCCHHHHCCCCCCCEEEEECCCCCCCCCCEE
VGHITPEAMEGGEIALVEDGDVISINARTNTLDVKVSAEDLEKRRKKWKKPPYRVTSGYL
EEECCHHHCCCCCEEEEECCCEEEEECCCEEEEEEECHHHHHHHHHHHCCCCCCCCHHHH
WKYIQMVKDASTGCLTDR
HHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA