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The map label for this gene is degP [H]

Identifier: 183222548

GI number: 183222548

Start: 3319192

End: 3320649

Strand: Reverse

Name: degP [H]

Synonym: LEPBI_I3203

Alternate gene names: 183222548

Gene position: 3320649-3319192 (Counterclockwise)

Preceding gene: 183222549

Following gene: 183222547

Centisome position: 92.25

GC content: 35.25

Gene sequence:

>1458_bases
ATGAATAAGATTATAATCGTTTGTTTTACCATCCTAATTTGGAGTCATTCCGCTTTTTCACAAACGGAATCAGAGCCAGC
TGTGGATTCTATTTTTCGCTCAGTGGTTCTCATCCGGAATGAAGGTTTTAACACAGAAAATAAAACCCAACCTTGGATGA
AAAAAAATCTCTACACTGGATTTGGATCAGGACTCGTTTTGCCAAACCAAACGATTTTGACCAATGCACATGTCGTTCGA
GATGCAAAACGGATTTTGGTAAGAAGTAGTTTTACAAAAAAGGAATACTTAGCTGATGTTAAATTCATAGGATATGATTG
TGATTTAGCATTATTACAAGTGAATGATCCTGATTTTGCAGAACAAACTACTTCATTAACCTTATTAGAAGGAATTCCAA
ACTTAGGTTCTGATGTACTATTACTTGGTTTTCCGAACGGAAATGATAGTTTATCAGTAGAAAAAGGATCCATCCTTCGC
TTCGAAAAAAATCGATACACCTATTCCGGGTTAGATTATCGTAATGTATTAAAAATATCTGCCAACATCCAACCTGGGAA
TTCAGGTGGGCCAGCTGTTCAAAATGGAAAAGTGGTAGGTCTTGTGTTTCAAATCAGCACTTTGGAACAGGGAATTGCTT
ATCTCATTTCGAATGATATCATTCGTCATTTTTTGGAAGATATAAATGATGGAAAGTATGATGGCTTCCCAAACATAGGA
TTCACCTTTCAGAACGGAAATCCAAAAAGTTTAAAACAAGCAATGAAAGTTCCGGCTTCGGAAACTGGAATTTTTGTGAA
TCGTATTTATCCTTCTTCCACATTCTCGAAAGTGCTGAAGGAAAAAGATTTTGTAACAGCATTTGACGGAATCCCTATCT
CCAACGATGGGGAGTTAAAATTGTCCAATAAAAAAGAATTCATTATCGATTGGATTGAAGACAAACAATTGAATTCAAAA
GTAACGATTAGTTTTTATCGAGCCGGTAAACAAAACCAGGCCGAAGTAAACTTACAAAAAAATTACGCTCTAGAATTATA
TCGCGATGCAACCGAAGACTATTTTTTACAAGCAGGGTTTGTGTTCCAACCAATCACCAGATCTTTTTTCCATTCAGAGG
ATGGTGATTTAGATAGTTCGTTACAATACCACTATAGTTATTTCATTCAAGATCTACTTTATCGGTATACCACTAGAGAC
ATTGTTCTTAGTTATGCATTTAGCGATCCTGAAACTTCCAAATATAAAAAATACAAATACAAAGTAGTAGAATCTATCAA
TGGGAAAGTGCCAAAAGATTTGAATGAATTTAAGTCGATTTGGAAAGAGAATAAACGTGGAATGATCGTCCTAAAATTTC
GTGGAATGGACTTACCGATTGTACTGCGCCCCGAATCCATTTACCAAATGAACCAAAGAGTCAAAAAAAGATATGGTGCT
AATTATGAAGAACTATAA

Upstream 100 bases:

>100_bases
ATTTGGAGAGACCCCAAAGTTGGGGAACGAGTTTTAGGAAAAGCTCTCCTTAAAAAAATTTCAGAAACTCAAATCATTTG
GGAGAAAGTCATTTTGTATT

Downstream 100 bases:

>100_bases
GTCGTTTGTATTACTTTTTTTATTCGTTTTTACTGCATCAAATCTTATTGCAGAAGAATTCGAAGACAAACGAGTCATTG
AGTCTCGAGTCACCTTTCAA

Product: putative serine-type endopeptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MNKIIIVCFTILIWSHSAFSQTESEPAVDSIFRSVVLIRNEGFNTENKTQPWMKKNLYTGFGSGLVLPNQTILTNAHVVR
DAKRILVRSSFTKKEYLADVKFIGYDCDLALLQVNDPDFAEQTTSLTLLEGIPNLGSDVLLLGFPNGNDSLSVEKGSILR
FEKNRYTYSGLDYRNVLKISANIQPGNSGGPAVQNGKVVGLVFQISTLEQGIAYLISNDIIRHFLEDINDGKYDGFPNIG
FTFQNGNPKSLKQAMKVPASETGIFVNRIYPSSTFSKVLKEKDFVTAFDGIPISNDGELKLSNKKEFIIDWIEDKQLNSK
VTISFYRAGKQNQAEVNLQKNYALELYRDATEDYFLQAGFVFQPITRSFFHSEDGDLDSSLQYHYSYFIQDLLYRYTTRD
IVLSYAFSDPETSKYKKYKYKVVESINGKVPKDLNEFKSIWKENKRGMIVLKFRGMDLPIVLRPESIYQMNQRVKKRYGA
NYEEL

Sequences:

>Translated_485_residues
MNKIIIVCFTILIWSHSAFSQTESEPAVDSIFRSVVLIRNEGFNTENKTQPWMKKNLYTGFGSGLVLPNQTILTNAHVVR
DAKRILVRSSFTKKEYLADVKFIGYDCDLALLQVNDPDFAEQTTSLTLLEGIPNLGSDVLLLGFPNGNDSLSVEKGSILR
FEKNRYTYSGLDYRNVLKISANIQPGNSGGPAVQNGKVVGLVFQISTLEQGIAYLISNDIIRHFLEDINDGKYDGFPNIG
FTFQNGNPKSLKQAMKVPASETGIFVNRIYPSSTFSKVLKEKDFVTAFDGIPISNDGELKLSNKKEFIIDWIEDKQLNSK
VTISFYRAGKQNQAEVNLQKNYALELYRDATEDYFLQAGFVFQPITRSFFHSEDGDLDSSLQYHYSYFIQDLLYRYTTRD
IVLSYAFSDPETSKYKKYKYKVVESINGKVPKDLNEFKSIWKENKRGMIVLKFRGMDLPIVLRPESIYQMNQRVKKRYGA
NYEEL
>Mature_485_residues
MNKIIIVCFTILIWSHSAFSQTESEPAVDSIFRSVVLIRNEGFNTENKTQPWMKKNLYTGFGSGLVLPNQTILTNAHVVR
DAKRILVRSSFTKKEYLADVKFIGYDCDLALLQVNDPDFAEQTTSLTLLEGIPNLGSDVLLLGFPNGNDSLSVEKGSILR
FEKNRYTYSGLDYRNVLKISANIQPGNSGGPAVQNGKVVGLVFQISTLEQGIAYLISNDIIRHFLEDINDGKYDGFPNIG
FTFQNGNPKSLKQAMKVPASETGIFVNRIYPSSTFSKVLKEKDFVTAFDGIPISNDGELKLSNKKEFIIDWIEDKQLNSK
VTISFYRAGKQNQAEVNLQKNYALELYRDATEDYFLQAGFVFQPITRSFFHSEDGDLDSSLQYHYSYFIQDLLYRYTTRD
IVLSYAFSDPETSKYKKYKYKVVESINGKVPKDLNEFKSIWKENKRGMIVLKFRGMDLPIVLRPESIYQMNQRVKKRYGA
NYEEL

Specific function: Serine protease that is required at high temperature. Involved in the degradation of damaged proteins. Shared specificity with hhoA/degQ [H]

COG id: COG0265

COG function: function code O; Trypsin-like serine proteases, typically periplasmic, contain C-terminal PDZ domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PDZ (DHR) domains [H]

Homologues:

Organism=Escherichia coli, GI1786356, Length=294, Percent_Identity=26.530612244898, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001478
- InterPro:   IPR009003
- InterPro:   IPR011782
- InterPro:   IPR001254
- InterPro:   IPR001940 [H]

Pfam domain/function: PF00595 PDZ; PF00089 Trypsin [H]

EC number: 3.4.21.- [C]

Molecular weight: Translated: 55352; Mature: 55352

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKIIIVCFTILIWSHSAFSQTESEPAVDSIFRSVVLIRNEGFNTENKTQPWMKKNLYTG
CCCEEEEEHHHHHHCCCHHCCCCCCHHHHHHHHHEEEEECCCCCCCCCCCCHHHHCCCCC
FGSGLVLPNQTILTNAHVVRDAKRILVRSSFTKKEYLADVKFIGYDCDLALLQVNDPDFA
CCCCEECCCCEEECCHHHHHHHHHHHHHCCCCHHHHHCCEEEEEECCCEEEEEECCCCHH
EQTTSLTLLEGIPNLGSDVLLLGFPNGNDSLSVEKGSILRFEKNRYTYSGLDYRNVLKIS
HHHHCEEEECCCCCCCCCEEEEEECCCCCCEEEECCCEEEEECCCEEECCCCCCEEEEEE
ANIQPGNSGGPAVQNGKVVGLVFQISTLEQGIAYLISNDIIRHFLEDINDGKYDGFPNIG
EEECCCCCCCCCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
FTFQNGNPKSLKQAMKVPASETGIFVNRIYPSSTFSKVLKEKDFVTAFDGIPISNDGELK
EEECCCCHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHCCCCEEEECCEECCCCCCEE
LSNKKEFIIDWIEDKQLNSKVTISFYRAGKQNQAEVNLQKNYALELYRDATEDYFLQAGF
ECCCCEEEEEEECCCCCCCEEEEEEEECCCCCCEEEEEECCEEEEEECCCCHHHEEECCC
VFQPITRSFFHSEDGDLDSSLQYHYSYFIQDLLYRYTTRDIVLSYAFSDPETSKYKKYKY
EEHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHH
KVVESINGKVPKDLNEFKSIWKENKRGMIVLKFRGMDLPIVLRPESIYQMNQRVKKRYGA
HHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCEEEECCHHHHHHHHHHHHHHCC
NYEEL
CCCCC
>Mature Secondary Structure
MNKIIIVCFTILIWSHSAFSQTESEPAVDSIFRSVVLIRNEGFNTENKTQPWMKKNLYTG
CCCEEEEEHHHHHHCCCHHCCCCCCHHHHHHHHHEEEEECCCCCCCCCCCCHHHHCCCCC
FGSGLVLPNQTILTNAHVVRDAKRILVRSSFTKKEYLADVKFIGYDCDLALLQVNDPDFA
CCCCEECCCCEEECCHHHHHHHHHHHHHCCCCHHHHHCCEEEEEECCCEEEEEECCCCHH
EQTTSLTLLEGIPNLGSDVLLLGFPNGNDSLSVEKGSILRFEKNRYTYSGLDYRNVLKIS
HHHHCEEEECCCCCCCCCEEEEEECCCCCCEEEECCCEEEEECCCEEECCCCCCEEEEEE
ANIQPGNSGGPAVQNGKVVGLVFQISTLEQGIAYLISNDIIRHFLEDINDGKYDGFPNIG
EEECCCCCCCCCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
FTFQNGNPKSLKQAMKVPASETGIFVNRIYPSSTFSKVLKEKDFVTAFDGIPISNDGELK
EEECCCCHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHCCCCEEEECCEECCCCCCEE
LSNKKEFIIDWIEDKQLNSKVTISFYRAGKQNQAEVNLQKNYALELYRDATEDYFLQAGF
ECCCCEEEEEEECCCCCCCEEEEEEEECCCCCCEEEEEECCEEEEEECCCCHHHEEECCC
VFQPITRSFFHSEDGDLDSSLQYHYSYFIQDLLYRYTTRDIVLSYAFSDPETSKYKKYKY
EEHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHH
KVVESINGKVPKDLNEFKSIWKENKRGMIVLKFRGMDLPIVLRPESIYQMNQRVKKRYGA
HHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCEEEECCHHHHHHHHHHHHHHCC
NYEEL
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: Serine endopeptidases [C]

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1645840; 11677609 [H]