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The map label for this gene is acsA1 [H]

Identifier: 183222441

GI number: 183222441

Start: 3206891

End: 3208849

Strand: Reverse

Name: acsA1 [H]

Synonym: LEPBI_I3095

Alternate gene names: 183222441

Gene position: 3208849-3206891 (Counterclockwise)

Preceding gene: 183222448

Following gene: 183222440

Centisome position: 89.14

GC content: 36.6

Gene sequence:

>1959_bases
ATGGCCGAAAAAGCACTATGGACTCCCGTTAACCATACTACGAATCTCACTCAATTCCAAAATGTTTTGGAGAAGAAACT
AGGAATATCATTTCCTGATTATGTTTCTTTTCATAAGTTTTCAACAGATAATACCGAGGTTTTTTGGAATGAGTGGCTTC
TCGAATCAGGATTCATATTGAGATCAAAACCATCAAAGACTTTGGAGAGAGGCAGTCACTTCGCAAAAAGTAAATGGTTT
CCAGGTGCTTTTTACAATTTTGCAGAGAATTTATTGGAAAAGGGAGATTCCCAAAAAGAAGCAATTGTATTTTATGCTGA
GGATGGAACCGTACAAAGGTTAACGTTTCAAAAATTAAAAAATGAAGTGATCAAACTTCAAAATCATTTGCTCTCACTTG
GTGTCAAAAAGGGCGATCGAGTTGTAGGTATCGTTCCAAATGCACCCATCTCTACCATCGGAATGTTAGCAACCACATCA
TTAGGTGCTATATGGTCGAGTGCTTCGCCTGATTTTGGAGTGAAGGGAATTCTTGATCGATTTGAACAAATATTACCAAA
AGTGGTTCTATCAGTAGAATCCTATTTGTTTAAAGGCAAAAAGATTTCTATTATCGATAAGTTAGAAGAAGTCACACAGT
CACTTTCATCTGCGAAGCATTCAGAATTTTTACAAACCATTATGTATGATTTTGTCGAACCCAAAGTTGATTTTGGAAAA
ATCAAAAATCCAATTCGGTACGAAGATCTAAAAGATCCTGAAAATTTGGAAATCCAATACACTCCCATCGAATTTTCTGA
TCCAGTCTACATCATGTTTTCTTCTGGTACAACTGGATTACCAAAATGTATCGTTCAGGGTGGAGGAGTTCTTTTGAATC
ATACGAAAGAATTATCTTTGCACTGTAATCTTTCAAGTGGAGAAAAATTGTTTTATTATACAACTTGTGGTTGGATGATG
TGGAACTGGTCTCAATCAGTCCTTGCATTGGGTGCAACCTTATACCAGTTTGACGGGAATCCCTTTTATCCAAATTGGGA
GACTCTTTGGAGTATGGCAGAGAAGGAAAACATCCAAGTTTTTGGAACAAGTGCAAAATATTTATCTGTGTTAGAAGAAG
AGGGAATCTCTGTAAAAGAAAAATATTTACTTCCGAATCTAAAAGTAATTCTTTCTACTGGATCTCCATTACCTATTTCT
GGTTTTCAATATGTTTATGAGAAGATCAATCCAAAGATCCAACTTTCTTCCATTTCTGGCGGCACTGACTTAAATGGATG
TTTTGCTTTGGGAAATCCCAGCTTACCTGTGTTTGCTGGTCAAATCCAATGTAAGGGACTTGGAATGGATGTACAAGTGT
TTGACGATATGGGTAAATCTGTTGTTGGTGAAAAAGGAGAATTGGTTTGTCCCACTCCATTTCCTTCTATGCCTCTTTAT
TTCTGGAATGATGAATCAGGTAATAAATACAAATCTGCTTATTTTGAAACCTATGTTAACATTTGGTGCCATGGTGATTT
TGCATCGATTACTCCCGAAAATGGTCTGATCATCTATGGCAGATCAGATGCAACTCTCAATCCAGGAGGAGTTCGGATTG
GAACGGCCGATATCTATTCTGTTGTCGCCAAAATTCCCGAAATCAAAGATTCTGTGATCATCGGTCAAGATTATAAAGAT
GATGTAAGAGTGATCTTATTTGTAGTTCTATCAAATGGTATCCAATTGGACGAGGTACTGATAAAAAAAATCAAAGAACA
AATTAAATTGGAAACGTCTCCAAGGCATGTCCCTTCTTTGATTCTCACAGTTCCCGAAATTCCTTATACGGTGAATGGGA
AAAAGGTTGAAATTGCCGTTAAACAGACAGTAGCTGGTATTGAAGTCAAAAACAAAAATGCGCTCGCTAATCCGAATGCT
CTTGATTTTTTCAAAAATAGAAAAGAATTAATGCAATGA

Upstream 100 bases:

>100_bases
AGAGCATTTTCCCAAGAATTTCTCTAATATTTTTTTCTACCAGTCATTTATGCAAACATACTTGCCTAGATATTCAAATT
CAGAAAGATCAGTTCTCAAG

Downstream 100 bases:

>100_bases
AATTTTTTAAGTTTTCACTTCTCGTTATTTCATTTGGATATCTTGGTTTGAATCCTTTAGTGGCTGACGTGGAAAAATGG
AAAACTTGTTTGCACTCCTA

Product: acetoacetyl-coenzyme A synthetase

Products: NA

Alternate protein names: Acetoacetate--CoA ligase 1; Acyl-activating enzyme 1 [H]

Number of amino acids: Translated: 652; Mature: 651

Protein sequence:

>652_residues
MAEKALWTPVNHTTNLTQFQNVLEKKLGISFPDYVSFHKFSTDNTEVFWNEWLLESGFILRSKPSKTLERGSHFAKSKWF
PGAFYNFAENLLEKGDSQKEAIVFYAEDGTVQRLTFQKLKNEVIKLQNHLLSLGVKKGDRVVGIVPNAPISTIGMLATTS
LGAIWSSASPDFGVKGILDRFEQILPKVVLSVESYLFKGKKISIIDKLEEVTQSLSSAKHSEFLQTIMYDFVEPKVDFGK
IKNPIRYEDLKDPENLEIQYTPIEFSDPVYIMFSSGTTGLPKCIVQGGGVLLNHTKELSLHCNLSSGEKLFYYTTCGWMM
WNWSQSVLALGATLYQFDGNPFYPNWETLWSMAEKENIQVFGTSAKYLSVLEEEGISVKEKYLLPNLKVILSTGSPLPIS
GFQYVYEKINPKIQLSSISGGTDLNGCFALGNPSLPVFAGQIQCKGLGMDVQVFDDMGKSVVGEKGELVCPTPFPSMPLY
FWNDESGNKYKSAYFETYVNIWCHGDFASITPENGLIIYGRSDATLNPGGVRIGTADIYSVVAKIPEIKDSVIIGQDYKD
DVRVILFVVLSNGIQLDEVLIKKIKEQIKLETSPRHVPSLILTVPEIPYTVNGKKVEIAVKQTVAGIEVKNKNALANPNA
LDFFKNRKELMQ

Sequences:

>Translated_652_residues
MAEKALWTPVNHTTNLTQFQNVLEKKLGISFPDYVSFHKFSTDNTEVFWNEWLLESGFILRSKPSKTLERGSHFAKSKWF
PGAFYNFAENLLEKGDSQKEAIVFYAEDGTVQRLTFQKLKNEVIKLQNHLLSLGVKKGDRVVGIVPNAPISTIGMLATTS
LGAIWSSASPDFGVKGILDRFEQILPKVVLSVESYLFKGKKISIIDKLEEVTQSLSSAKHSEFLQTIMYDFVEPKVDFGK
IKNPIRYEDLKDPENLEIQYTPIEFSDPVYIMFSSGTTGLPKCIVQGGGVLLNHTKELSLHCNLSSGEKLFYYTTCGWMM
WNWSQSVLALGATLYQFDGNPFYPNWETLWSMAEKENIQVFGTSAKYLSVLEEEGISVKEKYLLPNLKVILSTGSPLPIS
GFQYVYEKINPKIQLSSISGGTDLNGCFALGNPSLPVFAGQIQCKGLGMDVQVFDDMGKSVVGEKGELVCPTPFPSMPLY
FWNDESGNKYKSAYFETYVNIWCHGDFASITPENGLIIYGRSDATLNPGGVRIGTADIYSVVAKIPEIKDSVIIGQDYKD
DVRVILFVVLSNGIQLDEVLIKKIKEQIKLETSPRHVPSLILTVPEIPYTVNGKKVEIAVKQTVAGIEVKNKNALANPNA
LDFFKNRKELMQ
>Mature_651_residues
AEKALWTPVNHTTNLTQFQNVLEKKLGISFPDYVSFHKFSTDNTEVFWNEWLLESGFILRSKPSKTLERGSHFAKSKWFP
GAFYNFAENLLEKGDSQKEAIVFYAEDGTVQRLTFQKLKNEVIKLQNHLLSLGVKKGDRVVGIVPNAPISTIGMLATTSL
GAIWSSASPDFGVKGILDRFEQILPKVVLSVESYLFKGKKISIIDKLEEVTQSLSSAKHSEFLQTIMYDFVEPKVDFGKI
KNPIRYEDLKDPENLEIQYTPIEFSDPVYIMFSSGTTGLPKCIVQGGGVLLNHTKELSLHCNLSSGEKLFYYTTCGWMMW
NWSQSVLALGATLYQFDGNPFYPNWETLWSMAEKENIQVFGTSAKYLSVLEEEGISVKEKYLLPNLKVILSTGSPLPISG
FQYVYEKINPKIQLSSISGGTDLNGCFALGNPSLPVFAGQIQCKGLGMDVQVFDDMGKSVVGEKGELVCPTPFPSMPLYF
WNDESGNKYKSAYFETYVNIWCHGDFASITPENGLIIYGRSDATLNPGGVRIGTADIYSVVAKIPEIKDSVIIGQDYKDD
VRVILFVVLSNGIQLDEVLIKKIKEQIKLETSPRHVPSLILTVPEIPYTVNGKKVEIAVKQTVAGIEVKNKNALANPNAL
DFFKNRKELMQ

Specific function: Involved in poly-3-hydroxybutyrate degradation. Activates acetoacetate to acetoacetyl-CoA [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI31982927, Length=659, Percent_Identity=42.0333839150228, Blast_Score=534, Evalue=1e-151,
Organism=Homo sapiens, GI28416953, Length=592, Percent_Identity=24.8310810810811, Blast_Score=161, Evalue=2e-39,
Organism=Homo sapiens, GI8923896, Length=664, Percent_Identity=21.3855421686747, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI13375727, Length=646, Percent_Identity=23.5294117647059, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI253970489, Length=678, Percent_Identity=21.8289085545723, Blast_Score=129, Evalue=9e-30,
Organism=Homo sapiens, GI38505220, Length=554, Percent_Identity=22.7436823104693, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI42544132, Length=554, Percent_Identity=21.841155234657, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI122937307, Length=558, Percent_Identity=22.4014336917563, Blast_Score=66, Evalue=1e-10,
Organism=Escherichia coli, GI1790505, Length=651, Percent_Identity=23.1950844854071, Blast_Score=170, Evalue=3e-43,
Organism=Escherichia coli, GI1786529, Length=651, Percent_Identity=21.6589861751152, Blast_Score=122, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI17569507, Length=650, Percent_Identity=38.6153846153846, Blast_Score=457, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI71983001, Length=640, Percent_Identity=23.4375, Blast_Score=130, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI71982997, Length=640, Percent_Identity=23.4375, Blast_Score=130, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6319264, Length=645, Percent_Identity=24.6511627906977, Blast_Score=154, Evalue=5e-38,
Organism=Saccharomyces cerevisiae, GI6323182, Length=641, Percent_Identity=22.6209048361934, Blast_Score=140, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24667955, Length=658, Percent_Identity=25.3799392097264, Blast_Score=179, Evalue=4e-45,
Organism=Drosophila melanogaster, GI62472339, Length=570, Percent_Identity=25.2631578947368, Blast_Score=159, Evalue=8e-39,
Organism=Drosophila melanogaster, GI21355225, Length=612, Percent_Identity=24.1830065359477, Blast_Score=145, Evalue=8e-35,
Organism=Drosophila melanogaster, GI24667959, Length=525, Percent_Identity=24.3809523809524, Blast_Score=135, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005914
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.16 [H]

Molecular weight: Translated: 72917; Mature: 72785

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS00455 AMP_BINDING ; PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEKALWTPVNHTTNLTQFQNVLEKKLGISFPDYVSFHKFSTDNTEVFWNEWLLESGFIL
CCCCCEECCCCCCCCHHHHHHHHHHHHCCCCCCHHCEEEECCCCCEEEEEHHHHHCCCEE
RSKPSKTLERGSHFAKSKWFPGAFYNFAENLLEKGDSQKEAIVFYAEDGTVQRLTFQKLK
ECCCHHHHHHCCHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEHHHHHHHH
NEVIKLQNHLLSLGVKKGDRVVGIVPNAPISTIGMLATTSLGAIWSSASPDFGVKGILDR
HHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
FEQILPKVVLSVESYLFKGKKISIIDKLEEVTQSLSSAKHSEFLQTIMYDFVEPKVDFGK
HHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
IKNPIRYEDLKDPENLEIQYTPIEFSDPVYIMFSSGTTGLPKCIVQGGGVLLNHTKELSL
CCCCCCCCCCCCCCCCEEEEECEECCCCEEEEECCCCCCCCHHHHCCCCEEEECCEEEEE
HCNLSSGEKLFYYTTCGWMMWNWSQSVLALGATLYQFDGNPFYPNWETLWSMAEKENIQV
EEECCCCCEEEEEEEECEEEECCCHHHHHHCCEEEEECCCCCCCCHHHHHHHHCCCCEEE
FGTSAKYLSVLEEEGISVKEKYLLPNLKVILSTGSPLPISGFQYVYEKINPKIQLSSISG
EECCHHHHHHHHHCCCCEEHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCEEEEECCC
GTDLNGCFALGNPSLPVFAGQIQCKGLGMDVQVFDDMGKSVVGEKGELVCPTPFPSMPLY
CCCCCCEEEECCCCCCEEEEEEEEEECCCEEHHHHHCCHHHCCCCCCEECCCCCCCCCEE
FWNDESGNKYKSAYFETYVNIWCHGDFASITPENGLIIYGRSDATLNPGGVRIGTADIYS
EEECCCCCHHHHHHHEEEEEEEECCCCCCCCCCCCEEEEECCCCEECCCCEEECHHHHHH
VVAKIPEIKDSVIIGQDYKDDVRVILFVVLSNGIQLDEVLIKKIKEQIKLETSPRHVPSL
HHHHCCCCCCCEEECCCCCCHHHHEEEEHHHCCCCHHHHHHHHHHHHHEECCCCCCCCEE
ILTVPEIPYTVNGKKVEIAVKQTVAGIEVKNKNALANPNALDFFKNRKELMQ
EEECCCCCEEECCCEEEEEEEEEEECEEEECCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
AEKALWTPVNHTTNLTQFQNVLEKKLGISFPDYVSFHKFSTDNTEVFWNEWLLESGFIL
CCCCEECCCCCCCCHHHHHHHHHHHHCCCCCCHHCEEEECCCCCEEEEEHHHHHCCCEE
RSKPSKTLERGSHFAKSKWFPGAFYNFAENLLEKGDSQKEAIVFYAEDGTVQRLTFQKLK
ECCCHHHHHHCCHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEHHHHHHHH
NEVIKLQNHLLSLGVKKGDRVVGIVPNAPISTIGMLATTSLGAIWSSASPDFGVKGILDR
HHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
FEQILPKVVLSVESYLFKGKKISIIDKLEEVTQSLSSAKHSEFLQTIMYDFVEPKVDFGK
HHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
IKNPIRYEDLKDPENLEIQYTPIEFSDPVYIMFSSGTTGLPKCIVQGGGVLLNHTKELSL
CCCCCCCCCCCCCCCCEEEEECEECCCCEEEEECCCCCCCCHHHHCCCCEEEECCEEEEE
HCNLSSGEKLFYYTTCGWMMWNWSQSVLALGATLYQFDGNPFYPNWETLWSMAEKENIQV
EEECCCCCEEEEEEEECEEEECCCHHHHHHCCEEEEECCCCCCCCHHHHHHHHCCCCEEE
FGTSAKYLSVLEEEGISVKEKYLLPNLKVILSTGSPLPISGFQYVYEKINPKIQLSSISG
EECCHHHHHHHHHCCCCEEHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCEEEEECCC
GTDLNGCFALGNPSLPVFAGQIQCKGLGMDVQVFDDMGKSVVGEKGELVCPTPFPSMPLY
CCCCCCEEEECCCCCCEEEEEEEEEECCCEEHHHHHCCHHHCCCCCCEECCCCCCCCCEE
FWNDESGNKYKSAYFETYVNIWCHGDFASITPENGLIIYGRSDATLNPGGVRIGTADIYS
EEECCCCCHHHHHHHEEEEEEEECCCCCCCCCCCCEEEEECCCCEECCCCEEECHHHHHH
VVAKIPEIKDSVIIGQDYKDDVRVILFVVLSNGIQLDEVLIKKIKEQIKLETSPRHVPSL
HHHHCCCCCCCEEECCCCCCHHHHEEEEHHHCCCCHHHHHHHHHHHHHEECCCCCCCCEE
ILTVPEIPYTVNGKKVEIAVKQTVAGIEVKNKNALANPNALDFFKNRKELMQ
EEECCCCCEEECCCEEEEEEEEEEECEEEECCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10735852; 11481430 [H]