Click here to switch to the map view.

The map label for this gene is mrdA [H]

Identifier: 183222421

GI number: 183222421

Start: 3184559

End: 3186496

Strand: Direct

Name: mrdA [H]

Synonym: LEPBI_I3075

Alternate gene names: 183222421

Gene position: 3184559-3186496 (Clockwise)

Preceding gene: 183222420

Following gene: 183222422

Centisome position: 88.47

GC content: 41.64

Gene sequence:

>1938_bases
ATGAGCCAGTCGGCATCTGAGTTTCGTTTAGAAGCAAGTTTTCGGAAACGATTGTATTTTTTTACGGGAATGATTGTATT
CACGTTAACTGCGTACATTTTACAATTATTCAACTTACAAATTGTACAAGGCAGTGAAAACTCTCTCAAAGCCGAACGAT
TTGTCCGCCGAAGTGAATCCATTCCTGCTGATCGTGGCAATATTTTTGATCGAAATTTTTTAACTCCAGAAACAAGCCAA
CCCCTTGTGTCCAACTCAGCATCCCTGGATGTGATTTTAAACACGAGTTTACTCAAAAACGATGCCAAAAAAGTAAAAGA
ATACATTTATAAATTCTGCGAAGCACTTTCCATACCCATTGTTTATTACGAAAAGGAATTACAAGAATCCCGCCTCATCA
AAAAAATCCGTTCACGGGAACCTTTTGTTTTACTCGAAGGGATCTCAAGAGAACAACAAGAACGGATTTTAGTATTAGAT
AATATCAATCGTTATGTGTATTTGGTTTCTTCACCCGCCCGCGTGTACCATATGGGACCTGCACTTTCGCATGTGACCGG
TTATGTGGGAAAACCTACTACAAGTGACTTACAAGAAAAAGAAATCAAAACCTACCAATTGATTGGAAAAGGTGGAATCG
AATCATTGTATGACACAACCCTTCGTGGCCAAGACGGATTTCGCATCCAAAAACGAAACACAGAAGGCAATATCGAAGAA
GAACGAGTCATCGAACACTCTGTCCCAGGAAATAATTTAATATTAACCATTGATCGTGACATGCAAATTGCCGCTTACAA
AGCCCTAAAGGGTGTTAGGGGAACAGTACTCGCCATCAAAGCAACAACTGGTGAAGTACTGGCAATGGCATCAAACCCAT
CATATGATCCCAATATTTTGTCTGGGAAAAATAAATTGGAAAGGTCCATTCATTTTGGTCGCGTCACAAACAATGGTGGG
TTTTTGAACTTAGCCATCCAGTCTAGGTTCCCGCCTGCCTCCACTTTCAAAACATTGGTGGGCCTTGCTGCCATGGAAAG
TGAACACAAAATCAATTATGATCCGAAACAAACGTTTTCTTGCCCTGCTAGTTTTACCTTAAAGTCCACGTTCAAAGGGG
TTCCTGACCAAGTTTTTTATAACTGGGATAAAAAGAACCATGGTGAGTTAAACTTAGCACAAGCCTTAGAAAAATCCAAC
TCAGTTTATTTTTACCAATTGGGATATAAGCTGGGAGCAGAACCAATTCTCGCCTATTCCCGGTTATTTGGTTTGGACAA
AAAAACAGGCATTGATTTACCAGGAGAAGCCACTGGATTCATTCCAAGTTCCGATTGGAAAAAACGAACCTATGGCAACA
AATGGTTTGATGGGGATACCGTAAACTTATCCATCGGACAAGGATTCATATCTGTCACACCCATCGAGATGGCTTTGTTT
TACATGGCAGTTGTCAATAATGGAAAAATTTACAAACCGTACGTTGTGTCCGAAATCAGAAGTCCCCTAGACAACTCCCT
CATCCAAAAAACAGAACCAACCATCTTACGAGATATCCCATTAAAAAAATCCACTGTGGAAGCATTAAAAGAAGGATTGT
ATTTGGTCGGATATTCGGGAACTGCATCAGGAGTTTTAAATTCTCCAAACCTACCTGAAATTGCAGGAAAAACGGGAACA
GCGCAAACAAGAAGGAGAGGCGCATCTTCTTCCAACCACGCATGGTTCATTGGGTATGCACCAGTAAATGCACCCGTCGA
AAAACAAATATTAGTCGCTGCTTTCGTAGAATACGGGGTCGGTGGTGCGGCCTCAGCCGCTCCTGCGGCAAGGGAAGTCT
TTAAGGCCGCATTCCCACCAGGATCTTTCCCTAGAACCGATAGGTCCCGTGTGAAAACCATGGAAGAAGAAGCACCAGTC
GAACAAGAGGCATTTTAA

Upstream 100 bases:

>100_bases
ATTCGTTTTTGTATGTTTCCCTTTACAATGCATTCCTTGGGCCTGCGCTTTTCTTTTTGTTCTCGTGGGCGTATCGTTTG
GATGGGGATGAGGTGAGGCA

Downstream 100 bases:

>100_bases
TGGCTGATCGTAATACCGAAAAACTCGATTATTTTTTAATCTTTTCCGTTGTGCTCGTGGCAATGGCTGGAGTTCTCACA
CTCTACACACAAGAGGCCAA

Product: penicillin-binding protein

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 645; Mature: 644

Protein sequence:

>645_residues
MSQSASEFRLEASFRKRLYFFTGMIVFTLTAYILQLFNLQIVQGSENSLKAERFVRRSESIPADRGNIFDRNFLTPETSQ
PLVSNSASLDVILNTSLLKNDAKKVKEYIYKFCEALSIPIVYYEKELQESRLIKKIRSREPFVLLEGISREQQERILVLD
NINRYVYLVSSPARVYHMGPALSHVTGYVGKPTTSDLQEKEIKTYQLIGKGGIESLYDTTLRGQDGFRIQKRNTEGNIEE
ERVIEHSVPGNNLILTIDRDMQIAAYKALKGVRGTVLAIKATTGEVLAMASNPSYDPNILSGKNKLERSIHFGRVTNNGG
FLNLAIQSRFPPASTFKTLVGLAAMESEHKINYDPKQTFSCPASFTLKSTFKGVPDQVFYNWDKKNHGELNLAQALEKSN
SVYFYQLGYKLGAEPILAYSRLFGLDKKTGIDLPGEATGFIPSSDWKKRTYGNKWFDGDTVNLSIGQGFISVTPIEMALF
YMAVVNNGKIYKPYVVSEIRSPLDNSLIQKTEPTILRDIPLKKSTVEALKEGLYLVGYSGTASGVLNSPNLPEIAGKTGT
AQTRRRGASSSNHAWFIGYAPVNAPVEKQILVAAFVEYGVGGAASAAPAAREVFKAAFPPGSFPRTDRSRVKTMEEEAPV
EQEAF

Sequences:

>Translated_645_residues
MSQSASEFRLEASFRKRLYFFTGMIVFTLTAYILQLFNLQIVQGSENSLKAERFVRRSESIPADRGNIFDRNFLTPETSQ
PLVSNSASLDVILNTSLLKNDAKKVKEYIYKFCEALSIPIVYYEKELQESRLIKKIRSREPFVLLEGISREQQERILVLD
NINRYVYLVSSPARVYHMGPALSHVTGYVGKPTTSDLQEKEIKTYQLIGKGGIESLYDTTLRGQDGFRIQKRNTEGNIEE
ERVIEHSVPGNNLILTIDRDMQIAAYKALKGVRGTVLAIKATTGEVLAMASNPSYDPNILSGKNKLERSIHFGRVTNNGG
FLNLAIQSRFPPASTFKTLVGLAAMESEHKINYDPKQTFSCPASFTLKSTFKGVPDQVFYNWDKKNHGELNLAQALEKSN
SVYFYQLGYKLGAEPILAYSRLFGLDKKTGIDLPGEATGFIPSSDWKKRTYGNKWFDGDTVNLSIGQGFISVTPIEMALF
YMAVVNNGKIYKPYVVSEIRSPLDNSLIQKTEPTILRDIPLKKSTVEALKEGLYLVGYSGTASGVLNSPNLPEIAGKTGT
AQTRRRGASSSNHAWFIGYAPVNAPVEKQILVAAFVEYGVGGAASAAPAAREVFKAAFPPGSFPRTDRSRVKTMEEEAPV
EQEAF
>Mature_644_residues
SQSASEFRLEASFRKRLYFFTGMIVFTLTAYILQLFNLQIVQGSENSLKAERFVRRSESIPADRGNIFDRNFLTPETSQP
LVSNSASLDVILNTSLLKNDAKKVKEYIYKFCEALSIPIVYYEKELQESRLIKKIRSREPFVLLEGISREQQERILVLDN
INRYVYLVSSPARVYHMGPALSHVTGYVGKPTTSDLQEKEIKTYQLIGKGGIESLYDTTLRGQDGFRIQKRNTEGNIEEE
RVIEHSVPGNNLILTIDRDMQIAAYKALKGVRGTVLAIKATTGEVLAMASNPSYDPNILSGKNKLERSIHFGRVTNNGGF
LNLAIQSRFPPASTFKTLVGLAAMESEHKINYDPKQTFSCPASFTLKSTFKGVPDQVFYNWDKKNHGELNLAQALEKSNS
VYFYQLGYKLGAEPILAYSRLFGLDKKTGIDLPGEATGFIPSSDWKKRTYGNKWFDGDTVNLSIGQGFISVTPIEMALFY
MAVVNNGKIYKPYVVSEIRSPLDNSLIQKTEPTILRDIPLKKSTVEALKEGLYLVGYSGTASGVLNSPNLPEIAGKTGTA
QTRRRGASSSNHAWFIGYAPVNAPVEKQILVAAFVEYGVGGAASAAPAAREVFKAAFPPGSFPRTDRSRVKTMEEEAPVE
QEAF

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=651, Percent_Identity=28.7250384024578, Blast_Score=203, Evalue=3e-53,
Organism=Escherichia coli, GI1786272, Length=613, Percent_Identity=26.1011419249592, Blast_Score=114, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 71769; Mature: 71638

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSASEFRLEASFRKRLYFFTGMIVFTLTAYILQLFNLQIVQGSENSLKAERFVRRSES
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCHHHHHHHHHHHCC
IPADRGNIFDRNFLTPETSQPLVSNSASLDVILNTSLLKNDAKKVKEYIYKFCEALSIPI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCE
VYYEKELQESRLIKKIRSREPFVLLEGISREQQERILVLDNINRYVYLVSSPARVYHMGP
EEEHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEECCCEEEEEEECCCEEEECCC
ALSHVTGYVGKPTTSDLQEKEIKTYQLIGKGGIESLYDTTLRGQDGFRIQKRNTEGNIEE
HHHHHHCCCCCCCCCHHHHHHHHHEEEECCCCHHHHHHHHCCCCCCCEEEECCCCCCCCH
ERVIEHSVPGNNLILTIDRDMQIAAYKALKGVRGTVLAIKATTGEVLAMASNPSYDPNIL
HHHHHHCCCCCCEEEEECCCCHHHHHHHHCCCCCEEEEEEECCCEEEEEECCCCCCCCCC
SGKNKLERSIHFGRVTNNGGFLNLAIQSRFPPASTFKTLVGLAAMESEHKINYDPKQTFS
CCCHHHHHCEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCC
CPASFTLKSTFKGVPDQVFYNWDKKNHGELNLAQALEKSNSVYFYQLGYKLGAEPILAYS
CCCCEEEHHHHCCCCCCEEEECCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCHHHHHH
RLFGLDKKTGIDLPGEATGFIPSSDWKKRTYGNKWFDGDTVNLSIGQGFISVTPIEMALF
HHHCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCEEEEEECCCEEEECHHHHHHH
YMAVVNNGKIYKPYVVSEIRSPLDNSLIQKTEPTILRDIPLKKSTVEALKEGLYLVGYSG
HHHHHCCCCEECCHHHHHHHCHHHHHHHHCCCCCCEECCCCCHHHHHHHHCCEEEEEECC
TASGVLNSPNLPEIAGKTGTAQTRRRGASSSNHAWFIGYAPVNAPVEKQILVAAFVEYGV
CCCCCCCCCCCCHHCCCCCCHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCC
GGAASAAPAAREVFKAAFPPGSFPRTDRSRVKTMEEEAPVEQEAF
CCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHCCC
>Mature Secondary Structure 
SQSASEFRLEASFRKRLYFFTGMIVFTLTAYILQLFNLQIVQGSENSLKAERFVRRSES
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCHHHHHHHHHHHCC
IPADRGNIFDRNFLTPETSQPLVSNSASLDVILNTSLLKNDAKKVKEYIYKFCEALSIPI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCE
VYYEKELQESRLIKKIRSREPFVLLEGISREQQERILVLDNINRYVYLVSSPARVYHMGP
EEEHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEECCCEEEEEEECCCEEEECCC
ALSHVTGYVGKPTTSDLQEKEIKTYQLIGKGGIESLYDTTLRGQDGFRIQKRNTEGNIEE
HHHHHHCCCCCCCCCHHHHHHHHHEEEECCCCHHHHHHHHCCCCCCCEEEECCCCCCCCH
ERVIEHSVPGNNLILTIDRDMQIAAYKALKGVRGTVLAIKATTGEVLAMASNPSYDPNIL
HHHHHHCCCCCCEEEEECCCCHHHHHHHHCCCCCEEEEEEECCCEEEEEECCCCCCCCCC
SGKNKLERSIHFGRVTNNGGFLNLAIQSRFPPASTFKTLVGLAAMESEHKINYDPKQTFS
CCCHHHHHCEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCC
CPASFTLKSTFKGVPDQVFYNWDKKNHGELNLAQALEKSNSVYFYQLGYKLGAEPILAYS
CCCCEEEHHHHCCCCCCEEEECCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCHHHHHH
RLFGLDKKTGIDLPGEATGFIPSSDWKKRTYGNKWFDGDTVNLSIGQGFISVTPIEMALF
HHHCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCEEEEEECCCEEEECHHHHHHH
YMAVVNNGKIYKPYVVSEIRSPLDNSLIQKTEPTILRDIPLKKSTVEALKEGLYLVGYSG
HHHHHCCCCEECCHHHHHHHCHHHHHHHHCCCCCCEECCCCCHHHHHHHHCCEEEEEECC
TASGVLNSPNLPEIAGKTGTAQTRRRGASSSNHAWFIGYAPVNAPVEKQILVAAFVEYGV
CCCCCCCCCCCCHHCCCCCCHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCC
GGAASAAPAAREVFKAAFPPGSFPRTDRSRVKTMEEEAPVEQEAF
CCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]