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The map label for this gene is ntrB [H]

Identifier: 183222250

GI number: 183222250

Start: 2994390

End: 2996231

Strand: Direct

Name: ntrB [H]

Synonym: LEPBI_I2901

Alternate gene names: 183222250

Gene position: 2994390-2996231 (Clockwise)

Preceding gene: 183222245

Following gene: 183222252

Centisome position: 83.18

GC content: 37.84

Gene sequence:

>1842_bases
TTGTATACAATCCACATGCAAATACAAGTTTTCTTTCGTTCCAGCATCATTCTCCTCTCTTCTTTTGGTTGGTTTGCAAT
TGCTTACGGACTGATCCTATTTCAAGTTTCCTATCTTTTTACATTTTCTGTCCTATTTTTGGGTTTTCTCACTCTCTCAC
TTTTCCTTTTCCATCTGCCCACAAACCAAACCCTTTTGGAAGAAAAACCCAAAAAAAGAGATGAACTCGTACAAAATCTC
TTTGCTCTGGAAAAATTCAAAGAAGAGCTCATTTCCTTCAATGACCCTGACCAAATTAGCGAAACGATCAGCCAATTTTT
GGCTTCTAAAATACACGCAGAGTTTGTGCAAGTGTATACTTGGGATGAAAGGGAAGGACAATTTCGACCAAGACCATTTC
TCGATTCCAAAAACCAAAAACATCCAAACCTTCCGTCCATCCCTGTATTCAATCCATTTTTATTATGGTTATCCGAAAGA
GAAGGAATCCATATCAAAGAAAATTTCATCCAATATGTTTCTCCCAGTCATGAAAAAATTGCAAAAGAGGCCTTAGGTTT
TTTTTCAGACACCAAGTCGGAGTTAGTTGCAACACTTTCGATTAAATCTAGTTTGGTGGGCTTTATTCTACTTGGGAAAC
ACAAAGAAGGAAAAATTTACGATTTAGAAGAAATTGAAATCATTTTAGAAATCCTTTCCGTTTCTCTCATGTCACTATCC
AATTCGATGATTTACCAACAGTTGTTAAATTTAACGGAAACTTTGGAAGCGAAGGTTAGGGAAAGGACCAAAGAATTGGA
AGAAACGCAGGCCCACCTTGTCCAATCCGAAAAAATGGCATCTCTTGGGGTGATGGTGGCAGGGATTGCACATGAAATCA
ATACCCCTGCTGCTGTGATCAATGGTGCTGCCGATAATTTAGATGCCAATTTGGTCTTCGTTTTATCACACTTAGGTGAC
ATCACTCACCTCATTCAAAATCCTGATTTTCGTTCCCTTTATTTGGATATTTTATTTAGTTTTGTGAAAGAAGATCCAAC
GACCAAAATCGATCCAAAGGATAAATTCAAATTAAAACGAGAAACAAAATTAAAATTCATCCATGATGGAATGCCAGAAA
ATGATGCCACCGATTTATCCACGTTTCTCATCGACCAACATTTAATGCACATGCAAGAAGAACTTCTTCGGATTTGGAAA
TCTGGTGGAAAAGAAACCTTTGAAATGTTAAAAAACACCTTAAGTTTACAAAGGAATATCAAAAACATCAAATATGCGAT
TCGTAATATTGTACGAATTGTAAAAGCCTTAAAATACTATTCCCACTTAGGGCAAAATTCGTATGAAGTGTCTGACCTGC
ATGAAGGTTTGGAAAACACACTCGTGATCATGCAAAACCAAATCAAACATGGTGTCGAAATTGAACGAAATTACGGCACA
ATTCCGCCTGTTCGGTGTAATTTGGATGAACTGAACCAAGTGTGGACAAACCTAATTACAAACGCCATCCATGCAATGAA
ACGAAGCGATCATCCCAAACTCATCATATCATCAAGTCGGGTAGGAGAGGAGTATGTGATGATCAGTTTTGAAGACAATG
GATCGGGTATCCCAATGGAAATCAAAGATAAAATTTGGGATCCATTTTTTACAACAAAAGACCAAGGAGAAGGAACAGGG
CTTGGTTTAGGAATTGTGAAAGGGATCATTGAAAAACACAAAGGAAGGATTGAAGTAGAATCTTCTCCTGGACGAACCTT
GTTTATGGTCTATTTGCCGTTAGTTGGTCCAGGCGATGTTCCGAATCTCCCCAAAGAAATTTTTAGGGAGAGGAGAGGTT
AA

Upstream 100 bases:

>100_bases
CTCTTCTGAATAAATCTCGTAATACGCACCGAAGATACGAGCGATTGTAAATTGTTCTTTACCCAAGTGGAATCTGCACC
ATAATTATCTAGTTCTTACA

Downstream 100 bases:

>100_bases
TCGATCAAAAAAACAGTGGTTGGCTCAGAAGTTTTTCCCAGTTTTCTTTGGTAGTTTCAAATTTTTCTTTCGAAACCACT
GCTTCTTTTTCATTGGCCGT

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 613; Mature: 613

Protein sequence:

>613_residues
MYTIHMQIQVFFRSSIILLSSFGWFAIAYGLILFQVSYLFTFSVLFLGFLTLSLFLFHLPTNQTLLEEKPKKRDELVQNL
FALEKFKEELISFNDPDQISETISQFLASKIHAEFVQVYTWDEREGQFRPRPFLDSKNQKHPNLPSIPVFNPFLLWLSER
EGIHIKENFIQYVSPSHEKIAKEALGFFSDTKSELVATLSIKSSLVGFILLGKHKEGKIYDLEEIEIILEILSVSLMSLS
NSMIYQQLLNLTETLEAKVRERTKELEETQAHLVQSEKMASLGVMVAGIAHEINTPAAVINGAADNLDANLVFVLSHLGD
ITHLIQNPDFRSLYLDILFSFVKEDPTTKIDPKDKFKLKRETKLKFIHDGMPENDATDLSTFLIDQHLMHMQEELLRIWK
SGGKETFEMLKNTLSLQRNIKNIKYAIRNIVRIVKALKYYSHLGQNSYEVSDLHEGLENTLVIMQNQIKHGVEIERNYGT
IPPVRCNLDELNQVWTNLITNAIHAMKRSDHPKLIISSSRVGEEYVMISFEDNGSGIPMEIKDKIWDPFFTTKDQGEGTG
LGLGIVKGIIEKHKGRIEVESSPGRTLFMVYLPLVGPGDVPNLPKEIFRERRG

Sequences:

>Translated_613_residues
MYTIHMQIQVFFRSSIILLSSFGWFAIAYGLILFQVSYLFTFSVLFLGFLTLSLFLFHLPTNQTLLEEKPKKRDELVQNL
FALEKFKEELISFNDPDQISETISQFLASKIHAEFVQVYTWDEREGQFRPRPFLDSKNQKHPNLPSIPVFNPFLLWLSER
EGIHIKENFIQYVSPSHEKIAKEALGFFSDTKSELVATLSIKSSLVGFILLGKHKEGKIYDLEEIEIILEILSVSLMSLS
NSMIYQQLLNLTETLEAKVRERTKELEETQAHLVQSEKMASLGVMVAGIAHEINTPAAVINGAADNLDANLVFVLSHLGD
ITHLIQNPDFRSLYLDILFSFVKEDPTTKIDPKDKFKLKRETKLKFIHDGMPENDATDLSTFLIDQHLMHMQEELLRIWK
SGGKETFEMLKNTLSLQRNIKNIKYAIRNIVRIVKALKYYSHLGQNSYEVSDLHEGLENTLVIMQNQIKHGVEIERNYGT
IPPVRCNLDELNQVWTNLITNAIHAMKRSDHPKLIISSSRVGEEYVMISFEDNGSGIPMEIKDKIWDPFFTTKDQGEGTG
LGLGIVKGIIEKHKGRIEVESSPGRTLFMVYLPLVGPGDVPNLPKEIFRERRG
>Mature_613_residues
MYTIHMQIQVFFRSSIILLSSFGWFAIAYGLILFQVSYLFTFSVLFLGFLTLSLFLFHLPTNQTLLEEKPKKRDELVQNL
FALEKFKEELISFNDPDQISETISQFLASKIHAEFVQVYTWDEREGQFRPRPFLDSKNQKHPNLPSIPVFNPFLLWLSER
EGIHIKENFIQYVSPSHEKIAKEALGFFSDTKSELVATLSIKSSLVGFILLGKHKEGKIYDLEEIEIILEILSVSLMSLS
NSMIYQQLLNLTETLEAKVRERTKELEETQAHLVQSEKMASLGVMVAGIAHEINTPAAVINGAADNLDANLVFVLSHLGD
ITHLIQNPDFRSLYLDILFSFVKEDPTTKIDPKDKFKLKRETKLKFIHDGMPENDATDLSTFLIDQHLMHMQEELLRIWK
SGGKETFEMLKNTLSLQRNIKNIKYAIRNIVRIVKALKYYSHLGQNSYEVSDLHEGLENTLVIMQNQIKHGVEIERNYGT
IPPVRCNLDELNQVWTNLITNAIHAMKRSDHPKLIISSSRVGEEYVMISFEDNGSGIPMEIKDKIWDPFFTTKDQGEGTG
LGLGIVKGIIEKHKGRIEVESSPGRTLFMVYLPLVGPGDVPNLPKEIFRERRG

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=179, Percent_Identity=32.9608938547486, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1790436, Length=116, Percent_Identity=41.3793103448276, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1790300, Length=152, Percent_Identity=31.5789473684211, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1790346, Length=117, Percent_Identity=31.6239316239316, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 70217; Mature: 70217

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYTIHMQIQVFFRSSIILLSSFGWFAIAYGLILFQVSYLFTFSVLFLGFLTLSLFLFHLP
CEEEEEEEEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TNQTLLEEKPKKRDELVQNLFALEKFKEELISFNDPDQISETISQFLASKIHAEFVQVYT
CCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEE
WDEREGQFRPRPFLDSKNQKHPNLPSIPVFNPFLLWLSEREGIHIKENFIQYVSPSHEKI
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHEEECCCCCCHHHHHHHHHCCCHHHH
AKEALGFFSDTKSELVATLSIKSSLVGFILLGKHKEGKIYDLEEIEIILEILSVSLMSLS
HHHHHHHHHCCHHHHHHHHHHHHHHHEEHEEECCCCCCEECHHHHHHHHHHHHHHHHHHH
NSMIYQQLLNLTETLEAKVRERTKELEETQAHLVQSEKMASLGVMVAGIAHEINTPAAVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
NGAADNLDANLVFVLSHLGDITHLIQNPDFRSLYLDILFSFVKEDPTTKIDPKDKFKLKR
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
ETKLKFIHDGMPENDATDLSTFLIDQHLMHMQEELLRIWKSGGKETFEMLKNTLSLQRNI
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
KNIKYAIRNIVRIVKALKYYSHLGQNSYEVSDLHEGLENTLVIMQNQIKHGVEIERNYGT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCC
IPPVRCNLDELNQVWTNLITNAIHAMKRSDHPKLIISSSRVGEEYVMISFEDNGSGIPME
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEEEEECCCCCCCEE
IKDKIWDPFFTTKDQGEGTGLGLGIVKGIIEKHKGRIEVESSPGRTLFMVYLPLVGPGDV
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEEECCCCCCC
PNLPKEIFRERRG
CCCHHHHHHHCCC
>Mature Secondary Structure
MYTIHMQIQVFFRSSIILLSSFGWFAIAYGLILFQVSYLFTFSVLFLGFLTLSLFLFHLP
CEEEEEEEEEHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TNQTLLEEKPKKRDELVQNLFALEKFKEELISFNDPDQISETISQFLASKIHAEFVQVYT
CCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEE
WDEREGQFRPRPFLDSKNQKHPNLPSIPVFNPFLLWLSEREGIHIKENFIQYVSPSHEKI
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHEEECCCCCCHHHHHHHHHCCCHHHH
AKEALGFFSDTKSELVATLSIKSSLVGFILLGKHKEGKIYDLEEIEIILEILSVSLMSLS
HHHHHHHHHCCHHHHHHHHHHHHHHHEEHEEECCCCCCEECHHHHHHHHHHHHHHHHHHH
NSMIYQQLLNLTETLEAKVRERTKELEETQAHLVQSEKMASLGVMVAGIAHEINTPAAVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
NGAADNLDANLVFVLSHLGDITHLIQNPDFRSLYLDILFSFVKEDPTTKIDPKDKFKLKR
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
ETKLKFIHDGMPENDATDLSTFLIDQHLMHMQEELLRIWKSGGKETFEMLKNTLSLQRNI
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
KNIKYAIRNIVRIVKALKYYSHLGQNSYEVSDLHEGLENTLVIMQNQIKHGVEIERNYGT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCC
IPPVRCNLDELNQVWTNLITNAIHAMKRSDHPKLIISSSRVGEEYVMISFEDNGSGIPME
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEEEEECCCCCCCEE
IKDKIWDPFFTTKDQGEGTGLGLGIVKGIIEKHKGRIEVESSPGRTLFMVYLPLVGPGDV
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEEECCCCCCC
PNLPKEIFRERRG
CCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2574025 [H]