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The map label for this gene is narT [H]

Identifier: 183222128

GI number: 183222128

Start: 2869795

End: 2871150

Strand: Direct

Name: narT [H]

Synonym: LEPBI_I2771

Alternate gene names: 183222128

Gene position: 2869795-2871150 (Clockwise)

Preceding gene: 183222127

Following gene: 183222129

Centisome position: 79.72

GC content: 39.68

Gene sequence:

>1356_bases
GTGACGATTACACCTCATGCGAAAGCAACCAAGATCGATTTATTTAATTTCAAAACTCCCCAAATGAGGACATTCCACCT
CACTTGGATTGCATTTTTTTTGTGTTTTTTTGGATGGTTTGGAATCGCACCACTCATGGTTTATGTAAGAGAGGAACTCT
CTCTCACAAAGGCACAAATCGGAAACATCATCATTGCATCCGTTGCCATAACAATTTTTATGCGACTACTGATTGGATGG
TTGTGTGATAAAATAGGACCAAGAATTGCCTATACCTTTCTTTTAATTTTAGGTTCCATTCCTGTTATGAGTATTGGACT
AGCCGATAGTTACCTTTCCTTTTTATTATTACGGCTTGCGATCGGTGCCATCGGTGCATCGTTTGTCATCACTCAATACC
ATACTTCTGTTATGTTTGCACCAAACATCATTGGTACAGCAAATGCAACCACAGCTGGTTGGGGAAATTTAGGTGGTGGT
GTGACACAGATGGTGATGCCTCTGATCTTCGGATTCTTCGTTGCATTTGGATTTACAACAGGTGTTTCATGGAGACTTGC
CATGGTAGTTCCAGGGATTGCCTTATTTTTGATGGGGATACTGTATTACTTTGGAACGCAAGATACACCTGGTGGGAATT
TTAAAGATATCAAAGAAACTTATCCAACTTTCCAAGGTGGCAAAAAAAATTCACTCGCTAACTTCCTTTTGGTGATCAAA
GACTCTAGAGTTTGGTTATTATTTTTAGCGTATGGTGCTTGTTTCGGTATTGAATTAACAATTAATAATATAGCTGCCTT
GTATTATGTGGATCAGTTCAAATTAACACCCGCTACAGCAGGATTAATTGCAGGACTATTTGGTTTGATGAATTTGTTTG
CACGGACTCTCGGTGGAGTTTTCGGTGATAAATTTGGAATCAAATGGGGATTACGAGGTCGTGTGATTTGGTTATCTGCA
GCTCTTGCTGGCGAAGGGATTTGTTTGGTATTGTTTTCACAAATGACATCACTTGTTCTTGCAATCTCATCTATGATTGT
ATTTAGTTTGTTTGTTCAGATGTCTGAAGGTGCCACATTCTCTGTTGTTCCATTTGTGAATAAAAAAGCAATTGGTGCGG
TATCAGGAATTGTTGGTGCTGGCGGAAACGTAGGAGCAGTCTCTGCTGGATTTTTATTCCGTGGCGAGATGACATATCAA
AATGCCCTCCTAGTCATCGGTGTGGCCGTAACAATCTCTGCATTCTTCGCATTGTTAGTTCGTTTTACAATGGAAGAGGA
AAAAGAAGTTGGGGAAGAAATGAACAAAATCATTCCACAAGGTGAGAAAAAATCGGAATTAGTTACCGCTTCCTAA

Upstream 100 bases:

>100_bases
ATCGAAAAAACGCTGTGTTTTCGCTCTTTTTAGCAGATTCTCTTTTGGGACATTTGGTACGCTTTTTGCACTATGATTCT
ACATAAGGAGAATCATTCCC

Downstream 100 bases:

>100_bases
TCACAGAAACGAATACAATTTTCATTTGAGAGGCAATTTTACACTTGTCTCTCAAAAATTTCTTTTCTAAACTTCTTGTT
TTAGGAGAGAGATATGGACC

Product: nitrite extrusion protein 1 NarK

Products: Proton [Cytoplasm]; N-acetylneuraminate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MTITPHAKATKIDLFNFKTPQMRTFHLTWIAFFLCFFGWFGIAPLMVYVREELSLTKAQIGNIIIASVAITIFMRLLIGW
LCDKIGPRIAYTFLLILGSIPVMSIGLADSYLSFLLLRLAIGAIGASFVITQYHTSVMFAPNIIGTANATTAGWGNLGGG
VTQMVMPLIFGFFVAFGFTTGVSWRLAMVVPGIALFLMGILYYFGTQDTPGGNFKDIKETYPTFQGGKKNSLANFLLVIK
DSRVWLLFLAYGACFGIELTINNIAALYYVDQFKLTPATAGLIAGLFGLMNLFARTLGGVFGDKFGIKWGLRGRVIWLSA
ALAGEGICLVLFSQMTSLVLAISSMIVFSLFVQMSEGATFSVVPFVNKKAIGAVSGIVGAGGNVGAVSAGFLFRGEMTYQ
NALLVIGVAVTISAFFALLVRFTMEEEKEVGEEMNKIIPQGEKKSELVTAS

Sequences:

>Translated_451_residues
MTITPHAKATKIDLFNFKTPQMRTFHLTWIAFFLCFFGWFGIAPLMVYVREELSLTKAQIGNIIIASVAITIFMRLLIGW
LCDKIGPRIAYTFLLILGSIPVMSIGLADSYLSFLLLRLAIGAIGASFVITQYHTSVMFAPNIIGTANATTAGWGNLGGG
VTQMVMPLIFGFFVAFGFTTGVSWRLAMVVPGIALFLMGILYYFGTQDTPGGNFKDIKETYPTFQGGKKNSLANFLLVIK
DSRVWLLFLAYGACFGIELTINNIAALYYVDQFKLTPATAGLIAGLFGLMNLFARTLGGVFGDKFGIKWGLRGRVIWLSA
ALAGEGICLVLFSQMTSLVLAISSMIVFSLFVQMSEGATFSVVPFVNKKAIGAVSGIVGAGGNVGAVSAGFLFRGEMTYQ
NALLVIGVAVTISAFFALLVRFTMEEEKEVGEEMNKIIPQGEKKSELVTAS
>Mature_450_residues
TITPHAKATKIDLFNFKTPQMRTFHLTWIAFFLCFFGWFGIAPLMVYVREELSLTKAQIGNIIIASVAITIFMRLLIGWL
CDKIGPRIAYTFLLILGSIPVMSIGLADSYLSFLLLRLAIGAIGASFVITQYHTSVMFAPNIIGTANATTAGWGNLGGGV
TQMVMPLIFGFFVAFGFTTGVSWRLAMVVPGIALFLMGILYYFGTQDTPGGNFKDIKETYPTFQGGKKNSLANFLLVIKD
SRVWLLFLAYGACFGIELTINNIAALYYVDQFKLTPATAGLIAGLFGLMNLFARTLGGVFGDKFGIKWGLRGRVIWLSAA
LAGEGICLVLFSQMTSLVLAISSMIVFSLFVQMSEGATFSVVPFVNKKAIGAVSGIVGAGGNVGAVSAGFLFRGEMTYQN
ALLVIGVAVTISAFFALLVRFTMEEEKEVGEEMNKIIPQGEKKSELVTAS

Specific function: Probably required for nitrate uptake under anoxic conditions. Also possibly involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 48823; Mature: 48691

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTITPHAKATKIDLFNFKTPQMRTFHLTWIAFFLCFFGWFGIAPLMVYVREELSLTKAQI
CCCCCCCCCEEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GNIIIASVAITIFMRLLIGWLCDKIGPRIAYTFLLILGSIPVMSIGLADSYLSFLLLRLA
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHH
IGAIGASFVITQYHTSVMFAPNIIGTANATTAGWGNLGGGVTQMVMPLIFGFFVAFGFTT
HHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
GVSWRLAMVVPGIALFLMGILYYFGTQDTPGGNFKDIKETYPTFQGGKKNSLANFLLVIK
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCCEEEEEEEE
DSRVWLLFLAYGACFGIELTINNIAALYYVDQFKLTPATAGLIAGLFGLMNLFARTLGGV
CCCHHHHHHHHHHHEEEEEEHHCEEEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHH
FGDKFGIKWGLRGRVIWLSAALAGEGICLVLFSQMTSLVLAISSMIVFSLFVQMSEGATF
HCCCCCCEECCCCEEEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
SVVPFVNKKAIGAVSGIVGAGGNVGAVSAGFLFRGEMTYQNALLVIGVAVTISAFFALLV
EEECCCCCHHHHHHHHHHCCCCCCCCEEECEEEECCCHHCCHHHHHHHHHHHHHHHHHHH
RFTMEEEKEVGEEMNKIIPQGEKKSELVTAS
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TITPHAKATKIDLFNFKTPQMRTFHLTWIAFFLCFFGWFGIAPLMVYVREELSLTKAQI
CCCCCCCCEEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GNIIIASVAITIFMRLLIGWLCDKIGPRIAYTFLLILGSIPVMSIGLADSYLSFLLLRLA
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHH
IGAIGASFVITQYHTSVMFAPNIIGTANATTAGWGNLGGGVTQMVMPLIFGFFVAFGFTT
HHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
GVSWRLAMVVPGIALFLMGILYYFGTQDTPGGNFKDIKETYPTFQGGKKNSLANFLLVIK
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCCEEEEEEEE
DSRVWLLFLAYGACFGIELTINNIAALYYVDQFKLTPATAGLIAGLFGLMNLFARTLGGV
CCCHHHHHHHHHHHEEEEEEHHCEEEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHH
FGDKFGIKWGLRGRVIWLSAALAGEGICLVLFSQMTSLVLAISSMIVFSLFVQMSEGATF
HCCCCCCEECCCCEEEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
SVVPFVNKKAIGAVSGIVGAGGNVGAVSAGFLFRGEMTYQNALLVIGVAVTISAFFALLV
EEECCCCCHHHHHHHHHHCCCCCCCCEEECEEEECCCHHCCHHHHHHHHHHHHHHHHHHH
RFTMEEEKEVGEEMNKIIPQGEKKSELVTAS
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; N-acetylneuraminate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + N-acetylneuraminate [Periplasm] = Proton [Cytoplasm] + N-acetylneuraminate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9082911; 12426351 [H]