Click here to switch to the map view.

The map label for this gene is nirB [H]

Identifier: 183222123

GI number: 183222123

Start: 2863781

End: 2866300

Strand: Direct

Name: nirB [H]

Synonym: LEPBI_I2766

Alternate gene names: 183222123

Gene position: 2863781-2866300 (Clockwise)

Preceding gene: 183222121

Following gene: 183222124

Centisome position: 79.56

GC content: 38.41

Gene sequence:

>2520_bases
ATGATCAAGCGGAAGTTAGTTGTTATTGGGAATGGAATGGTGGGTCACAGGTTCTGCGAAAAATTAGTAGAATACGGTGG
AACAGACAAATTTGAAATCACTGTCCTTGGGGAAGAACCAAGACGCGCATATGACCGCGTACATCTCTCTGAATACTTTG
CCAATCGATCTGCGGAATCCTTGTATCTCTCTTCACCTGATTGGTATCGAACGAATGGAATTAAATTATTATTATCTGAG
CCTGCAGTGACATTAGATACTGTTAGACGAAAATTAGTCACAAGTTTAGGAACTGAATTAGAATTTGACGAATTAATTTT
TGCAACAGGATCCTCTCCCTTCATCCCACCATTAGAAGGATTAGACAAAGAAGGTGTATTTGTTTACCGTACGATAGAAG
ATCTTGAAGAAACTTTAGAATATAGTAAAAAAATAAAAAAGGCAGCTGTCCTCGGAGGAGGATTACTTGGTTTGGAAGCG
GCAAAGGCTCTTGTGGATTTAGGAAAAGAAACCCACGTGATTGAATTTGCGCCGAGGCTTATGCCACGCCAATTGGATGA
TGGTGGAGCTGCGATTCTAAAATCAAAAATTGAAGAAATTGGAGTTGAGATCCATTTAAACAAACAAACTGAAAAAGTTT
TAGGAGAGCAAAAAATTGAAGGATTTGCTTTCAAAGATGGCGGAACCTTAGACTTCGATTTGTTAATTGTATCTGCAGGG
ATTCGTCCAAGAGATGAATTGGCAAAAGAGGCTGGAATCGCTGTAGGCGATCGTGGTGGTATCATAGTTGATGATGGTAT
GGGTACGAATGTGTATGGAATTTATGCCATTGGGGAAGTTGCACTCCATAGAAACTTTATCTACGGTTTAGTAGCACCAG
GTTACGAAATGGCTGAAACCTTAGCCTTTAATCTTTGTAGTCCAGGCAACAAACCAAAAGTATATTCAGGATCCGATTTA
TCAACCAAACTGAAGTTAATTGGAGTTGAGGTTGCTTCATTTGGAGATGCACTTGGACAAAACGAACATATTCCGATTGT
TTTCAAAAACCCAAGAAGTGGAGTGTATAAGAAACTTGTCATTTCACCTGATGGAAAGTATTTGCTTGGTGGAATTCTTG
TGGGTGATGCAAAAGCATACGGGAACTTATTATCTTTTTATCTCAATAAAATGGAATTACCGGAAGAACCAGAAACATTG
ATTGTTGGATCTGTTTCTTCGGAAAATCTTTTTGGAGCTGATACTTTGCCGGATGAAGCAAAAATTTGTTCCTGCAATAA
TGTATCAAAAGGAGACATCCTAACAGCAATCCGAGAAAAAGAATGTTACGATATCACAAGCCTCAAAAATTGTTCGAAGG
CTGGAAGTGGATGCGGTGGTTGTTTGCCCCAAGTCAATTCGATTCTCAAAGCGGAATTAAAAACGCAAGGGAAAGTAGTC
ACCGAACACCTTTGCGAACATTTTAAATTTTCAAGAAAAGAACTTTACCAAGTCATTAAAGTAAAATCTCTCAAATCCTT
TCCTGATGTATTAAAAGAAGTGGGAAAAGGAAATGGATGCGAAATTTGTAAACCAGCTGTAGCTTCCATCATTGCGAGTA
TTTGGAATGAACCAATTCTAAAACATAGAGAAATCCAAGATACAAACGATAAGTATTTAGCCAACATCCAAAGAGGAGGG
ACTTATTCTGTCGTTCCAAGGATCCCTGGTGGTGAAATCACTCCTGACAAACTTATCGTCATTGGAGATGTCGCCAAAAA
ATACAATTTGTATTGTAAAATCACTGGTGGCCAACGCATCGATTTGTTAGGTGCAAGAATTGACCAACTACCATCAATTT
GGAAAGATTTGATAGATCATGGTTTTGAAAGTGGACATGCGTATGGAAAATCAATGCGAACTGTCAAAAGTTGTGTTGGT
TCCACCTGGTGTCGATTTGGCGTTCAAGATAGTACTTCATTTGCGATTAAACTTGAAGAGCGATACAAAGGAATTCGTGC
CCCTCATAAACTCAAATGTGGAGTCTCTGGCTGTATCCGTGAATGTGCAGAAGCACGCGGAAAAGATTTTGGTATCATCG
CAACTGAAAGAGGTTGGAACTTATACATCGGTGGGAACGGTGGAGTCAATCCAAAACATGCTATCTTATTTGCAGAAGAT
TTAGATGAAGATACATGTATCAAGTATATTGATCGTTATATGATGTTTTATATTAGAACAGCTGACAAACTCATGAGAAC
TTCTACTTGGTTAGAACAACTGGAAGGTGGCATTGAGTATTTGAAAGATGTTGTGATCAATGATCGACTTGGAATCAACT
CTCAGTTAGATGAAGAGATGAAACATTTGGTCAATACTTATCATTGTGAATGGAAAGATGTAGTAGAAGACCCTGAAAAA
CAAAAGAAATTCAAACACTTTGTGAATAGTAACGAAACAGATCCTAACATTCAATTTACGGAAGAACGTGGTCAAATTCG
ACCTGTGGATTGGGTTGAAAAAGACTTGGTGAATACTTAA

Upstream 100 bases:

>100_bases
TTTAAACTAGATGCCTTTCTTAGGTACACTTCGTGCATAAATATAGAGAAAGGAACCGTTGTTGGTACCTTTAGTTTGAA
TTCCTGGGTTAGGTAAAGAC

Downstream 100 bases:

>100_bases
AAGAGGTAAAAATCATATGCAAACAAATCAAAATACAAAAGAAAAAGTATTCATCGGTCCAGTTTCTGAATTTGAAAGAG
AAGGTGGGGTGAGTGCCAAA

Product: nitrite reductase large subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 839; Mature: 839

Protein sequence:

>839_residues
MIKRKLVVIGNGMVGHRFCEKLVEYGGTDKFEITVLGEEPRRAYDRVHLSEYFANRSAESLYLSSPDWYRTNGIKLLLSE
PAVTLDTVRRKLVTSLGTELEFDELIFATGSSPFIPPLEGLDKEGVFVYRTIEDLEETLEYSKKIKKAAVLGGGLLGLEA
AKALVDLGKETHVIEFAPRLMPRQLDDGGAAILKSKIEEIGVEIHLNKQTEKVLGEQKIEGFAFKDGGTLDFDLLIVSAG
IRPRDELAKEAGIAVGDRGGIIVDDGMGTNVYGIYAIGEVALHRNFIYGLVAPGYEMAETLAFNLCSPGNKPKVYSGSDL
STKLKLIGVEVASFGDALGQNEHIPIVFKNPRSGVYKKLVISPDGKYLLGGILVGDAKAYGNLLSFYLNKMELPEEPETL
IVGSVSSENLFGADTLPDEAKICSCNNVSKGDILTAIREKECYDITSLKNCSKAGSGCGGCLPQVNSILKAELKTQGKVV
TEHLCEHFKFSRKELYQVIKVKSLKSFPDVLKEVGKGNGCEICKPAVASIIASIWNEPILKHREIQDTNDKYLANIQRGG
TYSVVPRIPGGEITPDKLIVIGDVAKKYNLYCKITGGQRIDLLGARIDQLPSIWKDLIDHGFESGHAYGKSMRTVKSCVG
STWCRFGVQDSTSFAIKLEERYKGIRAPHKLKCGVSGCIRECAEARGKDFGIIATERGWNLYIGGNGGVNPKHAILFAED
LDEDTCIKYIDRYMMFYIRTADKLMRTSTWLEQLEGGIEYLKDVVINDRLGINSQLDEEMKHLVNTYHCEWKDVVEDPEK
QKKFKHFVNSNETDPNIQFTEERGQIRPVDWVEKDLVNT

Sequences:

>Translated_839_residues
MIKRKLVVIGNGMVGHRFCEKLVEYGGTDKFEITVLGEEPRRAYDRVHLSEYFANRSAESLYLSSPDWYRTNGIKLLLSE
PAVTLDTVRRKLVTSLGTELEFDELIFATGSSPFIPPLEGLDKEGVFVYRTIEDLEETLEYSKKIKKAAVLGGGLLGLEA
AKALVDLGKETHVIEFAPRLMPRQLDDGGAAILKSKIEEIGVEIHLNKQTEKVLGEQKIEGFAFKDGGTLDFDLLIVSAG
IRPRDELAKEAGIAVGDRGGIIVDDGMGTNVYGIYAIGEVALHRNFIYGLVAPGYEMAETLAFNLCSPGNKPKVYSGSDL
STKLKLIGVEVASFGDALGQNEHIPIVFKNPRSGVYKKLVISPDGKYLLGGILVGDAKAYGNLLSFYLNKMELPEEPETL
IVGSVSSENLFGADTLPDEAKICSCNNVSKGDILTAIREKECYDITSLKNCSKAGSGCGGCLPQVNSILKAELKTQGKVV
TEHLCEHFKFSRKELYQVIKVKSLKSFPDVLKEVGKGNGCEICKPAVASIIASIWNEPILKHREIQDTNDKYLANIQRGG
TYSVVPRIPGGEITPDKLIVIGDVAKKYNLYCKITGGQRIDLLGARIDQLPSIWKDLIDHGFESGHAYGKSMRTVKSCVG
STWCRFGVQDSTSFAIKLEERYKGIRAPHKLKCGVSGCIRECAEARGKDFGIIATERGWNLYIGGNGGVNPKHAILFAED
LDEDTCIKYIDRYMMFYIRTADKLMRTSTWLEQLEGGIEYLKDVVINDRLGINSQLDEEMKHLVNTYHCEWKDVVEDPEK
QKKFKHFVNSNETDPNIQFTEERGQIRPVDWVEKDLVNT
>Mature_839_residues
MIKRKLVVIGNGMVGHRFCEKLVEYGGTDKFEITVLGEEPRRAYDRVHLSEYFANRSAESLYLSSPDWYRTNGIKLLLSE
PAVTLDTVRRKLVTSLGTELEFDELIFATGSSPFIPPLEGLDKEGVFVYRTIEDLEETLEYSKKIKKAAVLGGGLLGLEA
AKALVDLGKETHVIEFAPRLMPRQLDDGGAAILKSKIEEIGVEIHLNKQTEKVLGEQKIEGFAFKDGGTLDFDLLIVSAG
IRPRDELAKEAGIAVGDRGGIIVDDGMGTNVYGIYAIGEVALHRNFIYGLVAPGYEMAETLAFNLCSPGNKPKVYSGSDL
STKLKLIGVEVASFGDALGQNEHIPIVFKNPRSGVYKKLVISPDGKYLLGGILVGDAKAYGNLLSFYLNKMELPEEPETL
IVGSVSSENLFGADTLPDEAKICSCNNVSKGDILTAIREKECYDITSLKNCSKAGSGCGGCLPQVNSILKAELKTQGKVV
TEHLCEHFKFSRKELYQVIKVKSLKSFPDVLKEVGKGNGCEICKPAVASIIASIWNEPILKHREIQDTNDKYLANIQRGG
TYSVVPRIPGGEITPDKLIVIGDVAKKYNLYCKITGGQRIDLLGARIDQLPSIWKDLIDHGFESGHAYGKSMRTVKSCVG
STWCRFGVQDSTSFAIKLEERYKGIRAPHKLKCGVSGCIRECAEARGKDFGIIATERGWNLYIGGNGGVNPKHAILFAED
LDEDTCIKYIDRYMMFYIRTADKLMRTSTWLEQLEGGIEYLKDVVINDRLGINSQLDEEMKHLVNTYHCEWKDVVEDPEK
QKKFKHFVNSNETDPNIQFTEERGQIRPVDWVEKDLVNT

Specific function: Nitrate assimilation (denitrification); second step. [C]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=275, Percent_Identity=28, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI21389617, Length=275, Percent_Identity=28, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI226437568, Length=275, Percent_Identity=28, Blast_Score=108, Evalue=2e-23,
Organism=Escherichia coli, GI1789765, Length=841, Percent_Identity=52.6753864447087, Blast_Score=880, Evalue=0.0,
Organism=Escherichia coli, GI1788892, Length=285, Percent_Identity=27.3684210526316, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI1789065, Length=317, Percent_Identity=23.9747634069401, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1789915, Length=227, Percent_Identity=29.5154185022026, Blast_Score=63, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17559934, Length=280, Percent_Identity=28.5714285714286, Blast_Score=94, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24585130, Length=284, Percent_Identity=27.112676056338, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI281359715, Length=318, Percent_Identity=26.4150943396226, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI281359713, Length=318, Percent_Identity=26.4150943396226, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24639250, Length=318, Percent_Identity=26.4150943396226, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI18543267, Length=318, Percent_Identity=26.4150943396226, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24639252, Length=318, Percent_Identity=26.4150943396226, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24639257, Length=318, Percent_Identity=26.4150943396226, Blast_Score=89, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR012748
- InterPro:   IPR017881
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327
- InterPro:   IPR017941 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 92974; Mature: 92974

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKRKLVVIGNGMVGHRFCEKLVEYGGTDKFEITVLGEEPRRAYDRVHLSEYFANRSAES
CCCEEEEEEECCCHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCE
LYLSSPDWYRTNGIKLLLSEPAVTLDTVRRKLVTSLGTELEFDELIFATGSSPFIPPLEG
EEECCCCCEECCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCC
LDKEGVFVYRTIEDLEETLEYSKKIKKAAVLGGGLLGLEAAKALVDLGKETHVIEFAPRL
CCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEHHHHH
MPRQLDDGGAAILKSKIEEIGVEIHLNKQTEKVLGEQKIEGFAFKDGGTLDFDLLIVSAG
CCCCCCCCHHHHHHHHHHHCCEEEEECCHHHHHHHHHHCCCEEECCCCCEEEEEEEEECC
IRPRDELAKEAGIAVGDRGGIIVDDGMGTNVYGIYAIGEVALHRNFIYGLVAPGYEMAET
CCCHHHHHHHCCCEECCCCCEEEECCCCCCEEEEEEHHHHHHHHHHHEEEECCCHHHHHH
LAFNLCSPGNKPKVYSGSDLSTKLKLIGVEVASFGDALGQNEHIPIVFKNPRSGVYKKLV
HHHHHCCCCCCCCEECCCCCCCEEEEEEEEHHHHHHHCCCCCCEEEEEECCCCCCEEEEE
ISPDGKYLLGGILVGDAKAYGNLLSFYLNKMELPEEPETLIVGSVSSENLFGADTLPDEA
ECCCCCEEEEEEEEECHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCC
KICSCNNVSKGDILTAIREKECYDITSLKNCSKAGSGCGGCLPQVNSILKAELKTQGKVV
CEECCCCCCCCHHHHHHHHCCHHCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHH
TEHLCEHFKFSRKELYQVIKVKSLKSFPDVLKEVGKGNGCEICKPAVASIIASIWNEPIL
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCH
KHREIQDTNDKYLANIQRGGTYSVVPRIPGGEITPDKLIVIGDVAKKYNLYCKITGGQRI
HCCCCCCCCCHHHEEECCCCEEEEEECCCCCCCCCCCEEEEECCCCCEEEEEEECCCCEE
DLLGARIDQLPSIWKDLIDHGFESGHAYGKSMRTVKSCVGSTWCRFGVQDSTSFAIKLEE
EHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCEEEEEEHH
RYKGIRAPHKLKCGVSGCIRECAEARGKDFGIIATERGWNLYIGGNGGVNPKHAILFAED
HHCCCCCCCCEECCHHHHHHHHHHHCCCCEEEEEECCCCEEEECCCCCCCCCEEEEEECC
LDEDTCIKYIDRYMMFYIRTADKLMRTSTWLEQLEGGIEYLKDVVINDRLGINSQLDEEM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
KHLVNTYHCEWKDVVEDPEKQKKFKHFVNSNETDPNIQFTEERGQIRPVDWVEKDLVNT
HHHHHHHCCCHHHHHCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCHHHHHHHCCC
>Mature Secondary Structure
MIKRKLVVIGNGMVGHRFCEKLVEYGGTDKFEITVLGEEPRRAYDRVHLSEYFANRSAES
CCCEEEEEEECCCHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCE
LYLSSPDWYRTNGIKLLLSEPAVTLDTVRRKLVTSLGTELEFDELIFATGSSPFIPPLEG
EEECCCCCEECCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCC
LDKEGVFVYRTIEDLEETLEYSKKIKKAAVLGGGLLGLEAAKALVDLGKETHVIEFAPRL
CCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEHHHHH
MPRQLDDGGAAILKSKIEEIGVEIHLNKQTEKVLGEQKIEGFAFKDGGTLDFDLLIVSAG
CCCCCCCCHHHHHHHHHHHCCEEEEECCHHHHHHHHHHCCCEEECCCCCEEEEEEEEECC
IRPRDELAKEAGIAVGDRGGIIVDDGMGTNVYGIYAIGEVALHRNFIYGLVAPGYEMAET
CCCHHHHHHHCCCEECCCCCEEEECCCCCCEEEEEEHHHHHHHHHHHEEEECCCHHHHHH
LAFNLCSPGNKPKVYSGSDLSTKLKLIGVEVASFGDALGQNEHIPIVFKNPRSGVYKKLV
HHHHHCCCCCCCCEECCCCCCCEEEEEEEEHHHHHHHCCCCCCEEEEEECCCCCCEEEEE
ISPDGKYLLGGILVGDAKAYGNLLSFYLNKMELPEEPETLIVGSVSSENLFGADTLPDEA
ECCCCCEEEEEEEEECHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCC
KICSCNNVSKGDILTAIREKECYDITSLKNCSKAGSGCGGCLPQVNSILKAELKTQGKVV
CEECCCCCCCCHHHHHHHHCCHHCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHH
TEHLCEHFKFSRKELYQVIKVKSLKSFPDVLKEVGKGNGCEICKPAVASIIASIWNEPIL
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCH
KHREIQDTNDKYLANIQRGGTYSVVPRIPGGEITPDKLIVIGDVAKKYNLYCKITGGQRI
HCCCCCCCCCHHHEEECCCCEEEEEECCCCCCCCCCCEEEEECCCCCEEEEEEECCCCEE
DLLGARIDQLPSIWKDLIDHGFESGHAYGKSMRTVKSCVGSTWCRFGVQDSTSFAIKLEE
EHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCEEEEEEHH
RYKGIRAPHKLKCGVSGCIRECAEARGKDFGIIATERGWNLYIGGNGGVNPKHAILFAED
HHCCCCCCCCEECCHHHHHHHHHHHCCCCEEEEEECCCCEEEECCCCCCCCCEEEEEECC
LDEDTCIKYIDRYMMFYIRTADKLMRTSTWLEQLEGGIEYLKDVVINDRLGINSQLDEEM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
KHLVNTYHCEWKDVVEDPEKQKKFKHFVNSNETDPNIQFTEERGQIRPVDWVEKDLVNT
HHHHHHHCCCHHHHHCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8468296 [H]