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The map label for this gene is recA

Identifier: 183221815

GI number: 183221815

Start: 2520970

End: 2522133

Strand: Direct

Name: recA

Synonym: LEPBI_I2446

Alternate gene names: 183221815

Gene position: 2520970-2522133 (Clockwise)

Preceding gene: 183221809

Following gene: 183221816

Centisome position: 70.03

GC content: 45.45

Gene sequence:

>1164_bases
ATGAAAAAAGAGAAAGCTGACAAGGCACTAGAAAAAGAAACAGACCAAAGAAAACAGGCAATTGATGCCGCCCTCGGCCA
AATTGAAAAACAATTCGGCAAAGGATCCATCATGCGTCTCGGTGCCGACACACGTATGTCGGAAATGAATGTGGTTTCCA
CAGGCTCTTTGGACCTTGACATTGCTCTTGGAATCGGTGGATTCCCATCCGGTCGTATCATCGAAATCTACGGACCAGAA
TCTTCTGGTAAAACGACTCTTACTCTCTCTGCCATAGCGGAGACACAAAAGAAAGGGGGCATTGCAGCCTTCATCGATGC
AGAACACGCACTCGATCCATCTTATGCAAAAAAACTCGGAGTCAACGTTGACGATCTCCTCGTTGCGCAACCTGACAATG
GTGAGGAAGCTTTGGAAATTTGCGAGTCACTCGTTCGTTCCAATGCCATTGACCTCATTGTCATCGACTCTGTCGCAGCC
CTTGTTCCCAAGGCAGAAATCGAAGGGGATATGGGAGACTCTCACATGGGCCTCCAAGCACGACTCATGTCACAAGCCCT
CCGTAAACTCACAGGTACCATTTCAAAATCCAGTACCACTGTCATTTTCATCAACCAAATCCGTATGAAAATTGGAGTCA
TGTTCGGAAGTCCAGAAACCACGACAGGTGGGAATGCTCTCAAATTTTATGCATCCATTCGCCTTGACATCCGCCGTATT
GAAACATTGAAAGAAAAAGAAGAACCCGTCGGAAACCGTGTGCGGGTGAAAGTGGTGAAAAACAAATGCGCCCCTCCTTT
CCGTCAGGCAGAATTTGATATTATGTATGCCAATGGGATCAACCGTGAAAGTTCACTGATTGATTTAGCCGTTCGCCATG
ATTTAGTCGCGAAAGCTGGTTCTTGGTATTCTTATAACGGCGAAAAGATAGGCCAAGGGAAAGAACAAGTAAGGAACTTC
TTTCTGGAAAATCCAGACATCGCTTTCAAAATTGAAAATCAAGTTCGGGATCTAAATGCGCTTCCCTTGCTTGACCAAGC
AAAAATCCAAACCCGAGAAGTGAAATCCATTGAAAGGGATACAAAAGAAACGAAGGAAACAAAATCAAAACAACCAGTGA
GTTTCTCGACCGAAGCTGATGGAGACATCGCCGTCGGTGAATAA

Upstream 100 bases:

>100_bases
TGGGTTCATCCTTGAGAGAGTCAGATGGATTTTTTGAAACCAACTGGCCAAGTCCCCTACCTAGTCTTTCGGAACACCGA
CTTAGGAGAACGATTGATTC

Downstream 100 bases:

>100_bases
CAAATATAGGCTTTCTTTTTAGGAACCATTGACCGGTTCGGGGTTTCCCGGGCCGGTTTTTTTTTGGAAATTTTCCCAAC
CATTTGAAAACGAAAAAAGA

Product: recombinase A

Products: NA

Alternate protein names: Recombinase A

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MKKEKADKALEKETDQRKQAIDAALGQIEKQFGKGSIMRLGADTRMSEMNVVSTGSLDLDIALGIGGFPSGRIIEIYGPE
SSGKTTLTLSAIAETQKKGGIAAFIDAEHALDPSYAKKLGVNVDDLLVAQPDNGEEALEICESLVRSNAIDLIVIDSVAA
LVPKAEIEGDMGDSHMGLQARLMSQALRKLTGTISKSSTTVIFINQIRMKIGVMFGSPETTTGGNALKFYASIRLDIRRI
ETLKEKEEPVGNRVRVKVVKNKCAPPFRQAEFDIMYANGINRESSLIDLAVRHDLVAKAGSWYSYNGEKIGQGKEQVRNF
FLENPDIAFKIENQVRDLNALPLLDQAKIQTREVKSIERDTKETKETKSKQPVSFSTEADGDIAVGE

Sequences:

>Translated_387_residues
MKKEKADKALEKETDQRKQAIDAALGQIEKQFGKGSIMRLGADTRMSEMNVVSTGSLDLDIALGIGGFPSGRIIEIYGPE
SSGKTTLTLSAIAETQKKGGIAAFIDAEHALDPSYAKKLGVNVDDLLVAQPDNGEEALEICESLVRSNAIDLIVIDSVAA
LVPKAEIEGDMGDSHMGLQARLMSQALRKLTGTISKSSTTVIFINQIRMKIGVMFGSPETTTGGNALKFYASIRLDIRRI
ETLKEKEEPVGNRVRVKVVKNKCAPPFRQAEFDIMYANGINRESSLIDLAVRHDLVAKAGSWYSYNGEKIGQGKEQVRNF
FLENPDIAFKIENQVRDLNALPLLDQAKIQTREVKSIERDTKETKETKSKQPVSFSTEADGDIAVGE
>Mature_387_residues
MKKEKADKALEKETDQRKQAIDAALGQIEKQFGKGSIMRLGADTRMSEMNVVSTGSLDLDIALGIGGFPSGRIIEIYGPE
SSGKTTLTLSAIAETQKKGGIAAFIDAEHALDPSYAKKLGVNVDDLLVAQPDNGEEALEICESLVRSNAIDLIVIDSVAA
LVPKAEIEGDMGDSHMGLQARLMSQALRKLTGTISKSSTTVIFINQIRMKIGVMFGSPETTTGGNALKFYASIRLDIRRI
ETLKEKEEPVGNRVRVKVVKNKCAPPFRQAEFDIMYANGINRESSLIDLAVRHDLVAKAGSWYSYNGEKIGQGKEQVRNF
FLENPDIAFKIENQVRDLNALPLLDQAKIQTREVKSIERDTKETKETKSKQPVSFSTEADGDIAVGE

Specific function: Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with lexA causing its activation

COG id: COG0468

COG function: function code L; RecA/RadA recombinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family

Homologues:

Organism=Escherichia coli, GI1789051, Length=325, Percent_Identity=67.6923076923077, Blast_Score=455, Evalue=1e-129,

Paralogues:

None

Copy number: 1548 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800-1200 (L-broth) 40,000-60,000 (L-broth + Nalidixate) 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RECA_LEPBA (B0SCX2)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001963442.1
- ProteinModelPortal:   B0SCX2
- SMR:   B0SCX2
- GeneID:   6387925
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_2374
- HOGENOM:   HBG339889
- OMA:   GRDNTIT
- ProtClustDB:   PRK09354
- BioCyc:   LBIF355278:LBF_2374-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00268
- InterPro:   IPR003593
- InterPro:   IPR013765
- InterPro:   IPR020584
- InterPro:   IPR020588
- InterPro:   IPR020587
- PANTHER:   PTHR22942:SF1
- PRINTS:   PR00142
- SMART:   SM00382
- TIGRFAMs:   TIGR02012

Pfam domain/function: PF00154 RecA

EC number: NA

Molecular weight: Translated: 42321; Mature: 42321

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS00321 RECA_1; PS50162 RECA_2; PS50163 RECA_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKEKADKALEKETDQRKQAIDAALGQIEKQFGKGSIMRLGADTRMSEMNVVSTGSLDLD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEECCCEEEE
IALGIGGFPSGRIIEIYGPESSGKTTLTLSAIAETQKKGGIAAFIDAEHALDPSYAKKLG
EEEECCCCCCCCEEEEECCCCCCCEEEEEHHHHHHHHCCCEEEEEECHHCCCHHHHHHHC
VNVDDLLVAQPDNGEEALEICESLVRSNAIDLIVIDSVAALVPKAEIEGDMGDSHMGLQA
CCHHHEEEECCCCCHHHHHHHHHHHHCCCCEEEEECHHHHHCCHHHCCCCCCCCCCCHHH
RLMSQALRKLTGTISKSSTTVIFINQIRMKIGVMFGSPETTTGGNALKFYASIRLDIRRI
HHHHHHHHHHHCCCCCCCEEEEEEEEEEEEEEEEECCCCCCCCCCEEEEEEEEHHHHHHH
ETLKEKEEPVGNRVRVKVVKNKCAPPFRQAEFDIMYANGINRESSLIDLAVRHDLVAKAG
HHHHHHCCCCCCEEEEEEEECCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCC
SWYSYNGEKIGQGKEQVRNFFLENPDIAFKIENQVRDLNALPLLDQAKIQTREVKSIERD
CEEEECCCCCCCCHHHHHHHHCCCCCEEEEECCHHHCCCCCCCCHHHHHHHHHHHHHHHH
TKETKETKSKQPVSFSTEADGDIAVGE
HHHHHHHCCCCCCEECCCCCCCEECCC
>Mature Secondary Structure
MKKEKADKALEKETDQRKQAIDAALGQIEKQFGKGSIMRLGADTRMSEMNVVSTGSLDLD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEECCCEEEE
IALGIGGFPSGRIIEIYGPESSGKTTLTLSAIAETQKKGGIAAFIDAEHALDPSYAKKLG
EEEECCCCCCCCEEEEECCCCCCCEEEEEHHHHHHHHCCCEEEEEECHHCCCHHHHHHHC
VNVDDLLVAQPDNGEEALEICESLVRSNAIDLIVIDSVAALVPKAEIEGDMGDSHMGLQA
CCHHHEEEECCCCCHHHHHHHHHHHHCCCCEEEEECHHHHHCCHHHCCCCCCCCCCCHHH
RLMSQALRKLTGTISKSSTTVIFINQIRMKIGVMFGSPETTTGGNALKFYASIRLDIRRI
HHHHHHHHHHHCCCCCCCEEEEEEEEEEEEEEEEECCCCCCCCCCEEEEEEEEHHHHHHH
ETLKEKEEPVGNRVRVKVVKNKCAPPFRQAEFDIMYANGINRESSLIDLAVRHDLVAKAG
HHHHHHCCCCCCEEEEEEEECCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCC
SWYSYNGEKIGQGKEQVRNFFLENPDIAFKIENQVRDLNALPLLDQAKIQTREVKSIERD
CEEEECCCCCCCCHHHHHHHHCCCCCEEEEECCHHHCCCCCCCCHHHHHHHHHHHHHHHH
TKETKETKSKQPVSFSTEADGDIAVGE
HHHHHHHCCCCCCEECCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA