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The map label for this gene is pcrA2 [H]

Identifier: 183221749

GI number: 183221749

Start: 2450345

End: 2451628

Strand: Direct

Name: pcrA2 [H]

Synonym: LEPBI_I2378

Alternate gene names: 183221749

Gene position: 2450345-2451628 (Clockwise)

Preceding gene: 183221748

Following gene: 183221750

Centisome position: 68.07

GC content: 36.76

Gene sequence:

>1284_bases
ATGTGGAGTGAAGAACAATCGAAAATCATTCATAGCACAGAGCCTGTCATCCAAGTCATTGCTGGTGCTGGCTCTGGAAA
AACTGCGACTATGATTGGTCTTTTGGAAAGAAGAGAAAAAAACGAAACCATCCTTCCCGAAGAGACTCTTGTTGTCACAT
TTACGAAAAAAGCCACACATGAATTTAAGGAAAGATGTCAAAAAAAAAGTCTTTCCAATTCCTATCACATTTCCACCTTT
CATTCGTTTTGTTACGATTCCTTAAAACAATTTGACCGAAATAAAAACTGGAAAAATTACAAACTACTCGGAGAATCAAA
AAAATGGGAAATCACAAAATATTTGTTATCTCCTTACAGAGATTTGATCGGCGGGATTCCCTTTTCCATTTTATTAAAAA
AAGATGGCGCCTATTTCAAATCCATTTCGGAAGAGATTTATGTTTCTTTTCGATCAAGTTTTCATCATTGGAAGGAAAAA
AATCATTATTTTGAATTCGATGATTTAATCCAAAATTTTTTAGAGTTTTTACATTCGAATCATTCATCTCTTATCAAAAA
AAAATGGAAGTCTCTCATCATTGACGAATTCCAAGATACTGATGAAATCCAATTGGAAATCATCAAACAAATGGAATTTG
ATCGAATCACAGTTGTAGGAGATGATTGGCAAGCTATCTATGGGTTCAGAGGGGCCACACCCAAACCTTTTTTAGAATTC
CCAATCCATTTTCCAAATGCTGTTCAATTTAAACTTTCAACTAACTACCGCTCCACTCATAAAATCATTCAAAAAAGTTT
ATTACCAATCCATCAAAACAAAGAAAAAATCCCAAAACAAACGATCTCTCACAGACATGAAAAAGGATTTTTCCATTTAG
AACGAAGTTTCAAAAAGGAACCCCAACTAAAATCTTTATGGAAACAATTCCAGTCACTTGATCCCAATTTGGTGATACTC
ACTCGGAGCAATTTTCGAAAAGCGGAGTGGATCCAAGCGGGAGTTCCTCTCTCACAAGTGATGACCATCCACAGTGCCAA
AGGTTTGGAATTCAAAACTGTCCTAGTCGACCTAAGCAAAGGCTGGAGTGAAGACCAAAATTTAGTAGATACGGAGGAGG
AAAGGAGGATTCTCTATGTGGCCCTATCGAGAGCCAAAGACAATTTGATCGTTCTCATCAATGCAACTTCGGATCCAAAA
AGTCTATGTGACCGATTCAGTGAGGATTTTTCCGTTTGGAATCGATTCAGAAATCGTACTTTACGGTGGACTAGACTCTG
GTGA

Upstream 100 bases:

>100_bases
TGGTTATTATGACCTCAAAGAAGAGGAAGATGAAGATACGGCTGTGTTTAAAATGTAATTTTTAAAGAACCAAAGATACA
ATCCTCCACCTTACCAATGT

Downstream 100 bases:

>100_bases
AGTGGTTTTACCTTGGGGGATTAGCTCAGTTGGTTAGAGCGCTACCTTGACATGGTAGAGGTCACTGGTTCGAACCCAGT
ATCTCCCAAGACCATTTTCT

Product: ATP-dependent DNA helicase PcrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MWSEEQSKIIHSTEPVIQVIAGAGSGKTATMIGLLERREKNETILPEETLVVTFTKKATHEFKERCQKKSLSNSYHISTF
HSFCYDSLKQFDRNKNWKNYKLLGESKKWEITKYLLSPYRDLIGGIPFSILLKKDGAYFKSISEEIYVSFRSSFHHWKEK
NHYFEFDDLIQNFLEFLHSNHSSLIKKKWKSLIIDEFQDTDEIQLEIIKQMEFDRITVVGDDWQAIYGFRGATPKPFLEF
PIHFPNAVQFKLSTNYRSTHKIIQKSLLPIHQNKEKIPKQTISHRHEKGFFHLERSFKKEPQLKSLWKQFQSLDPNLVIL
TRSNFRKAEWIQAGVPLSQVMTIHSAKGLEFKTVLVDLSKGWSEDQNLVDTEEERRILYVALSRAKDNLIVLINATSDPK
SLCDRFSEDFSVWNRFRNRTLRWTRLW

Sequences:

>Translated_427_residues
MWSEEQSKIIHSTEPVIQVIAGAGSGKTATMIGLLERREKNETILPEETLVVTFTKKATHEFKERCQKKSLSNSYHISTF
HSFCYDSLKQFDRNKNWKNYKLLGESKKWEITKYLLSPYRDLIGGIPFSILLKKDGAYFKSISEEIYVSFRSSFHHWKEK
NHYFEFDDLIQNFLEFLHSNHSSLIKKKWKSLIIDEFQDTDEIQLEIIKQMEFDRITVVGDDWQAIYGFRGATPKPFLEF
PIHFPNAVQFKLSTNYRSTHKIIQKSLLPIHQNKEKIPKQTISHRHEKGFFHLERSFKKEPQLKSLWKQFQSLDPNLVIL
TRSNFRKAEWIQAGVPLSQVMTIHSAKGLEFKTVLVDLSKGWSEDQNLVDTEEERRILYVALSRAKDNLIVLINATSDPK
SLCDRFSEDFSVWNRFRNRTLRWTRLW
>Mature_427_residues
MWSEEQSKIIHSTEPVIQVIAGAGSGKTATMIGLLERREKNETILPEETLVVTFTKKATHEFKERCQKKSLSNSYHISTF
HSFCYDSLKQFDRNKNWKNYKLLGESKKWEITKYLLSPYRDLIGGIPFSILLKKDGAYFKSISEEIYVSFRSSFHHWKEK
NHYFEFDDLIQNFLEFLHSNHSSLIKKKWKSLIIDEFQDTDEIQLEIIKQMEFDRITVVGDDWQAIYGFRGATPKPFLEF
PIHFPNAVQFKLSTNYRSTHKIIQKSLLPIHQNKEKIPKQTISHRHEKGFFHLERSFKKEPQLKSLWKQFQSLDPNLVIL
TRSNFRKAEWIQAGVPLSQVMTIHSAKGLEFKTVLVDLSKGWSEDQNLVDTEEERRILYVALSRAKDNLIVLINATSDPK
SLCDRFSEDFSVWNRFRNRTLRWTRLW

Specific function: DNA helicase. Has a broad nucleotide specificity, even being able to hydrolyze ethenonucleotides, and is able to couple the hydrolysis to unwinding of DNA substrates. It is a 3'-5' helicase but at high protein concentrations it can also displace a substra

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=301, Percent_Identity=28.9036544850498, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI2367296, Length=305, Percent_Identity=24.9180327868852, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1787196, Length=476, Percent_Identity=25, Blast_Score=91, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6322369, Length=313, Percent_Identity=29.7124600638978, Blast_Score=91, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 50447; Mature: 50447

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWSEEQSKIIHSTEPVIQVIAGAGSGKTATMIGLLERREKNETILPEETLVVTFTKKATH
CCCCHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEHHHHH
EFKERCQKKSLSNSYHISTFHSFCYDSLKQFDRNKNWKNYKLLGESKKWEITKYLLSPYR
HHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHH
DLIGGIPFSILLKKDGAYFKSISEEIYVSFRSSFHHWKEKNHYFEFDDLIQNFLEFLHSN
HHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC
HSSLIKKKWKSLIIDEFQDTDEIQLEIIKQMEFDRITVVGDDWQAIYGFRGATPKPFLEF
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHCCCCCCCCCCEEC
PIHFPNAVQFKLSTNYRSTHKIIQKSLLPIHQNKEKIPKQTISHRHEKGFFHLERSFKKE
CCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHCCHHHHHHHHCCC
PQLKSLWKQFQSLDPNLVILTRSNFRKAEWIQAGVPLSQVMTIHSAKGLEFKTVLVDLSK
CHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHCCCCHHHHHHHHCCCCCEEEEEEEEHHC
GWSEDQNLVDTEEERRILYVALSRAKDNLIVLINATSDPKSLCDRFSEDFSVWNRFRNRT
CCCCCCCCCCCHHHCEEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCC
LRWTRLW
CEEEECC
>Mature Secondary Structure
MWSEEQSKIIHSTEPVIQVIAGAGSGKTATMIGLLERREKNETILPEETLVVTFTKKATH
CCCCHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEHHHHH
EFKERCQKKSLSNSYHISTFHSFCYDSLKQFDRNKNWKNYKLLGESKKWEITKYLLSPYR
HHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHH
DLIGGIPFSILLKKDGAYFKSISEEIYVSFRSSFHHWKEKNHYFEFDDLIQNFLEFLHSN
HHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC
HSSLIKKKWKSLIIDEFQDTDEIQLEIIKQMEFDRITVVGDDWQAIYGFRGATPKPFLEF
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHCCCCCCCCCCEEC
PIHFPNAVQFKLSTNYRSTHKIIQKSLLPIHQNKEKIPKQTISHRHEKGFFHLERSFKKE
CCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHCCHHHHHHHHCCC
PQLKSLWKQFQSLDPNLVILTRSNFRKAEWIQAGVPLSQVMTIHSAKGLEFKTVLVDLSK
CHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHCCCCHHHHHHHHCCCCCEEEEEEEEHHC
GWSEDQNLVDTEEERRILYVALSRAKDNLIVLINATSDPKSLCDRFSEDFSVWNRFRNRT
CCCCCCCCCCCHHHCEEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCC
LRWTRLW
CEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9592155; 8934527; 10199404; 10388562; 10454638 [H]