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The map label for this gene is pcrA4 [H]

Identifier: 183221448

GI number: 183221448

Start: 2155278

End: 2157314

Strand: Reverse

Name: pcrA4 [H]

Synonym: LEPBI_I2066

Alternate gene names: 183221448

Gene position: 2157314-2155278 (Counterclockwise)

Preceding gene: 183221449

Following gene: 183221447

Centisome position: 59.93

GC content: 44.28

Gene sequence:

>2037_bases
ATGTCCCTTACTGGACCCCTTATGAAATTGAATGCGGCACAAATGGAAGCAGTCTCTACCATCCAAGGCCCTCTTCTTGT
CTTTGCTGGGGCAGGGTCAGGGAAAACGAGGGTCATCACCAACCGCATCGCACATATGGTGGAAGGGGTCAAAATCCCGG
CGAGTAAAATTGTTGCCCTTTCCTTCACCAATAAAAGTGCCAAAGAGATGGCGGAACGCCTTCGGAAAATGGTCCCTCGG
GAAAAACTGAAAGGGATCACACTTTCCACCTTTCATTCGTTAGGTTTAAAAATATTAAAAGAACATATCGGAAAACTAGG
GTATAACGAAACCTTTTTACTCTTCAATGGAACCGACCAAGAAGCCTTTGTTTCCGACCTTTTAAAGTCCAAACGACTTG
ATCCCAAAAAAGTCCCTCCCAGGGAAATTTTACGCCGGATCTCCTATGCCAAAAACACACAAGTCCATCCAAAAGATAAT
GGTCTCACAGGAGAGTTTGACCTTGTAGCCGCAGAAATTTTCCCTCTCTATGAGGATGGCTTAAAAGAAAAAAATGCCAT
TGACTTTGATGATTTGATTTTACTTCCGAAACGTCTGCTTGCTGAATTTCCAGAGATTGCTGCCTATTACCAAAGGAAAC
ATGAATACTTCCTAGTGGATGAATTCCAAGACACGAACCAATTGCAGTATGAATTTTTATCTCTCTTTCGTGGCAATAGT
GATAACCTTTGTGTGGTGGGGGATGACGACCAGAGTATCTATGCCTTCCGCGGTTCCAACGTACAACTCATTTTGAATTT
TGAAAGGGAATTCCCTCATGCCAAAGTGGTGAGGCTTCTCGAAAATTACCGTTCCACATCCCTTATCATCCAAGCCGCCA
ACTCCCTCATCCAAAACAACATTGGCCGAAAGGAAAAAACTCTCTATAGCCGGATCCCTTCCGCCGAACGCGTGGAATAC
TATGAAACGGCAGATGAAAGGGAAGAAGCGATTTTTGTGGCAGGAAGGATCCAAACTCTCCTCATCAAAAACGAATTCAA
AGGGAAAGAGATTGCCATCCTATTTCGAACGAACTTCCAATCCCGACCTTTTGAAGAGGAACTTCGGAACCGAAGCATTC
CGTACAAGGTAGTGGGTGGGTATAATTTTTTTGACCGAAAGGAAATCAGGGATTGTATCTCGTATCTTCGGTACGTCGCA
AACCCGAAAGATGATTATTCCCTCCTTCGGATCATCAACTATCCCAAACGTGGGATTGGTCCAGGGACCATGCAAAAACT
CCAAGAAGAGGCATTTACGCATAAACTCTCTCTCTATGAAATCTTTCATAAAATGATTGAGAGTCCTGATTATTTGCCCG
AAGTAAAGGCTAAGGTCAGACAAGAAATTTACCAATTTGTCGAAATGGTAGATGCCTTCAAAAAAAAGTTTGCGATGTCG
CAGAAATTGGCCCCTGTGCTTCGGGAGATGATCACCCAAATTGGGTTTGAACGGGAAATCTCCATGGAAGAGACCGAGGA
AAAGGTGGTGAAAGCCCGGATCTACAACCTGAGTGAACTTGTGAACATGTTGTCCTTTTTTGAAGAGGAAGAGGGCAGAG
AAGGAAAGGCCACCATCTTTGACTTCTTGCAAAGGCTCGTTCTCCTTATGGAAGACGAACCCAAAGAAGACGAGGAAGAC
CGTCGGGTGCAACTTCTCACGATGCACCAGTCTAAAGGACTCGAATACGATTTAGTATTTTTAGTGGGACTAGAAGAGGG
AATTTTACCGAACTCACGTGTTATAGAAGAAGAAGGGGAAGTGGTCGATGAAGAACGACGCCTTCTCTACGTGGGTATGA
CTCGCCCAAGACGAAAATTGTACTTGACTTCGGCTCGTACAAGACGCAAATTTGGGGAGCAAATCGAGAGTGCCCCCTCT
CGGTTTTTAAACGAGCTGTCTCAGGACGCTGTTCTTTTTTTCCCGATGGAAACGAAGGATAGAGACACAGAAACTAAGAA
TTTCTTAGAGGAATTAGACAAACTAAAGGTAGGCTAA

Upstream 100 bases:

>100_bases
TTTTAAATTTCCATGGCCCAAACAAATCATGGATGGAAACCGCTCCTAAGTAGACAACCAGAGTCTTTTTTCACTCGACC
CCAGATGATTTCACTGGATT

Downstream 100 bases:

>100_bases
TGAAATCGATTCTCCCACTCACCCTCCTATTGTTGGTAGTGGCATTTGAAAATTGCGCATCAAACAAGGAAACCATCCGC
ACAGGAGTTTCCAAAATCAA

Product: ATP-dependent DNA helicase PcrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 678; Mature: 677

Protein sequence:

>678_residues
MSLTGPLMKLNAAQMEAVSTIQGPLLVFAGAGSGKTRVITNRIAHMVEGVKIPASKIVALSFTNKSAKEMAERLRKMVPR
EKLKGITLSTFHSLGLKILKEHIGKLGYNETFLLFNGTDQEAFVSDLLKSKRLDPKKVPPREILRRISYAKNTQVHPKDN
GLTGEFDLVAAEIFPLYEDGLKEKNAIDFDDLILLPKRLLAEFPEIAAYYQRKHEYFLVDEFQDTNQLQYEFLSLFRGNS
DNLCVVGDDDQSIYAFRGSNVQLILNFEREFPHAKVVRLLENYRSTSLIIQAANSLIQNNIGRKEKTLYSRIPSAERVEY
YETADEREEAIFVAGRIQTLLIKNEFKGKEIAILFRTNFQSRPFEEELRNRSIPYKVVGGYNFFDRKEIRDCISYLRYVA
NPKDDYSLLRIINYPKRGIGPGTMQKLQEEAFTHKLSLYEIFHKMIESPDYLPEVKAKVRQEIYQFVEMVDAFKKKFAMS
QKLAPVLREMITQIGFEREISMEETEEKVVKARIYNLSELVNMLSFFEEEEGREGKATIFDFLQRLVLLMEDEPKEDEED
RRVQLLTMHQSKGLEYDLVFLVGLEEGILPNSRVIEEEGEVVDEERRLLYVGMTRPRRKLYLTSARTRRKFGEQIESAPS
RFLNELSQDAVLFFPMETKDRDTETKNFLEELDKLKVG

Sequences:

>Translated_678_residues
MSLTGPLMKLNAAQMEAVSTIQGPLLVFAGAGSGKTRVITNRIAHMVEGVKIPASKIVALSFTNKSAKEMAERLRKMVPR
EKLKGITLSTFHSLGLKILKEHIGKLGYNETFLLFNGTDQEAFVSDLLKSKRLDPKKVPPREILRRISYAKNTQVHPKDN
GLTGEFDLVAAEIFPLYEDGLKEKNAIDFDDLILLPKRLLAEFPEIAAYYQRKHEYFLVDEFQDTNQLQYEFLSLFRGNS
DNLCVVGDDDQSIYAFRGSNVQLILNFEREFPHAKVVRLLENYRSTSLIIQAANSLIQNNIGRKEKTLYSRIPSAERVEY
YETADEREEAIFVAGRIQTLLIKNEFKGKEIAILFRTNFQSRPFEEELRNRSIPYKVVGGYNFFDRKEIRDCISYLRYVA
NPKDDYSLLRIINYPKRGIGPGTMQKLQEEAFTHKLSLYEIFHKMIESPDYLPEVKAKVRQEIYQFVEMVDAFKKKFAMS
QKLAPVLREMITQIGFEREISMEETEEKVVKARIYNLSELVNMLSFFEEEEGREGKATIFDFLQRLVLLMEDEPKEDEED
RRVQLLTMHQSKGLEYDLVFLVGLEEGILPNSRVIEEEGEVVDEERRLLYVGMTRPRRKLYLTSARTRRKFGEQIESAPS
RFLNELSQDAVLFFPMETKDRDTETKNFLEELDKLKVG
>Mature_677_residues
SLTGPLMKLNAAQMEAVSTIQGPLLVFAGAGSGKTRVITNRIAHMVEGVKIPASKIVALSFTNKSAKEMAERLRKMVPRE
KLKGITLSTFHSLGLKILKEHIGKLGYNETFLLFNGTDQEAFVSDLLKSKRLDPKKVPPREILRRISYAKNTQVHPKDNG
LTGEFDLVAAEIFPLYEDGLKEKNAIDFDDLILLPKRLLAEFPEIAAYYQRKHEYFLVDEFQDTNQLQYEFLSLFRGNSD
NLCVVGDDDQSIYAFRGSNVQLILNFEREFPHAKVVRLLENYRSTSLIIQAANSLIQNNIGRKEKTLYSRIPSAERVEYY
ETADEREEAIFVAGRIQTLLIKNEFKGKEIAILFRTNFQSRPFEEELRNRSIPYKVVGGYNFFDRKEIRDCISYLRYVAN
PKDDYSLLRIINYPKRGIGPGTMQKLQEEAFTHKLSLYEIFHKMIESPDYLPEVKAKVRQEIYQFVEMVDAFKKKFAMSQ
KLAPVLREMITQIGFEREISMEETEEKVVKARIYNLSELVNMLSFFEEEEGREGKATIFDFLQRLVLLMEDEPKEDEEDR
RVQLLTMHQSKGLEYDLVFLVGLEEGILPNSRVIEEEGEVVDEERRLLYVGMTRPRRKLYLTSARTRRKFGEQIESAPSR
FLNELSQDAVLFFPMETKDRDTETKNFLEELDKLKVG

Specific function: DNA helicase. Has a broad nucleotide specificity, even being able to hydrolyze ethenonucleotides, and is able to couple the hydrolysis to unwinding of DNA substrates. It is a 3'-5' helicase but at high protein concentrations it can also displace a substra

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=650, Percent_Identity=40.1538461538462, Blast_Score=437, Evalue=1e-123,
Organism=Escherichia coli, GI2367296, Length=652, Percent_Identity=36.3496932515337, Blast_Score=404, Evalue=1e-113,
Organism=Escherichia coli, GI1787196, Length=344, Percent_Identity=28.7790697674419, Blast_Score=106, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6322369, Length=730, Percent_Identity=27.1232876712329, Blast_Score=229, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6324477, Length=683, Percent_Identity=23.5724743777452, Blast_Score=110, Evalue=9e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 78573; Mature: 78442

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLTGPLMKLNAAQMEAVSTIQGPLLVFAGAGSGKTRVITNRIAHMVEGVKIPASKIVAL
CCCCCCHHHCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCHHEEEE
SFTNKSAKEMAERLRKMVPREKLKGITLSTFHSLGLKILKEHIGKLGYNETFLLFNGTDQ
EECCHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCH
EAFVSDLLKSKRLDPKKVPPREILRRISYAKNTQVHPKDNGLTGEFDLVAAEIFPLYEDG
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
LKEKNAIDFDDLILLPKRLLAEFPEIAAYYQRKHEYFLVDEFQDTNQLQYEFLSLFRGNS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCC
DNLCVVGDDDQSIYAFRGSNVQLILNFEREFPHAKVVRLLENYRSTSLIIQAANSLIQNN
CCEEEECCCCCEEEEEECCCEEEEEEECHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHC
IGRKEKTLYSRIPSAERVEYYETADEREEAIFVAGRIQTLLIKNEFKGKEIAILFRTNFQ
CCHHHHHHHHHCCCHHHHHHHHCCCCHHCEEEEECHHEEEEEECCCCCCEEEEEEECCCC
SRPFEEELRNRSIPYKVVGGYNFFDRKEIRDCISYLRYVANPKDDYSLLRIINYPKRGIG
CCCHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCC
PGTMQKLQEEAFTHKLSLYEIFHKMIESPDYLPEVKAKVRQEIYQFVEMVDAFKKKFAMS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QKLAPVLREMITQIGFEREISMEETEEKVVKARIYNLSELVNMLSFFEEEEGREGKATIF
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
DFLQRLVLLMEDEPKEDEEDRRVQLLTMHQSKGLEYDLVFLVGLEEGILPNSRVIEEEGE
HHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCEEEEEEEECCCCCCCCCCEECCCCC
VVDEERRLLYVGMTRPRRKLYLTSARTRRKFGEQIESAPSRFLNELSQDAVLFFPMETKD
HHCCCCCEEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
RDTETKNFLEELDKLKVG
CCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SLTGPLMKLNAAQMEAVSTIQGPLLVFAGAGSGKTRVITNRIAHMVEGVKIPASKIVAL
CCCCCHHHCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCHHEEEE
SFTNKSAKEMAERLRKMVPREKLKGITLSTFHSLGLKILKEHIGKLGYNETFLLFNGTDQ
EECCHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCH
EAFVSDLLKSKRLDPKKVPPREILRRISYAKNTQVHPKDNGLTGEFDLVAAEIFPLYEDG
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
LKEKNAIDFDDLILLPKRLLAEFPEIAAYYQRKHEYFLVDEFQDTNQLQYEFLSLFRGNS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCC
DNLCVVGDDDQSIYAFRGSNVQLILNFEREFPHAKVVRLLENYRSTSLIIQAANSLIQNN
CCEEEECCCCCEEEEEECCCEEEEEEECHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHC
IGRKEKTLYSRIPSAERVEYYETADEREEAIFVAGRIQTLLIKNEFKGKEIAILFRTNFQ
CCHHHHHHHHHCCCHHHHHHHHCCCCHHCEEEEECHHEEEEEECCCCCCEEEEEEECCCC
SRPFEEELRNRSIPYKVVGGYNFFDRKEIRDCISYLRYVANPKDDYSLLRIINYPKRGIG
CCCHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCC
PGTMQKLQEEAFTHKLSLYEIFHKMIESPDYLPEVKAKVRQEIYQFVEMVDAFKKKFAMS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QKLAPVLREMITQIGFEREISMEETEEKVVKARIYNLSELVNMLSFFEEEEGREGKATIF
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
DFLQRLVLLMEDEPKEDEEDRRVQLLTMHQSKGLEYDLVFLVGLEEGILPNSRVIEEEGE
HHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCEEEEEEEECCCCCCCCCCEECCCCC
VVDEERRLLYVGMTRPRRKLYLTSARTRRKFGEQIESAPSRFLNELSQDAVLFFPMETKD
HHCCCCCEEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
RDTETKNFLEELDKLKVG
CCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9592155; 8934527; 10199404; 10388562; 10454638 [H]