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The map label for this gene is rmlB [H]

Identifier: 183221421

GI number: 183221421

Start: 2120252

End: 2121274

Strand: Reverse

Name: rmlB [H]

Synonym: LEPBI_I2039

Alternate gene names: 183221421

Gene position: 2121274-2120252 (Counterclockwise)

Preceding gene: 183221422

Following gene: 183221420

Centisome position: 58.93

GC content: 35.58

Gene sequence:

>1023_bases
ATGGAATTCAAGAATGTTTTGGTGACAGGGAGTGCCGGATTCATTGGATCCAATTTTGTTCCTTACTTTTTGGAAACACA
TCCCCATGTTCATGTTGTCAGTTTGGATAAAATTACGTATGCAGGGAATCTGGATAACTTAAGTTCTATTATTTCCGAAA
AACGACATACATTTGTAAATGGTGATATATGTGATGCAGAACTCGTCGGCTCATTGTTTGAAACATACAAGTTTGATTTG
GTTGTTCATTTTGCTGCGGAAAGTCATGTCGATAATTCCATAATAAATCCAAAGATCTTTTTAGAAACTAATGTGATTGG
AACGTTTCAGCTTTTGCAGACGGCCTATTCAACTTGGTTCTCGGCTCCGTTCCAACGAAAACCAGGATTTGAGGCGGCTA
AATTCATTCATATATCGACAGATGAAGTTTTTGGTTCTTTAGGTGAATCTGGGTTTTTCACAGAAGACTCACCTTATCAA
CCAAATTCACCATATAGCGCAAGTAAGGCTTCATCAGATCATATTGTAAGAAGTTATTATCATACCTATGGTTTGCCGAT
GATCATTACTAATTGTTCCAATAATTATGGACCAAAACAACATAATGAAAAATTGATCCCTACCATTATTAGAAACGCAC
TAGAGGACAAGAACATTCCGATTTATGGAACGGGAATGAATATACGAGACTGGCTTTTTGTCATGGATCATGTCAAAGGA
ATAGAAAAGGTGATTTTATATGGTAAACTTGGTGAGACATACAATATCGGTGGAAACAATGAACTTACAAACAACCAAGT
TGTGAATGTCATTTGTAATCATTTAGATAAAGTAATCCCAAGAGGAAACGGAAAATCATATTCGGAACTCATTATGTATG
TGTCCGATAGGCCTGGGCATGATAAACGTTATGCGATTGATGCAACTAAAATGAAAAAAGATCTGAATTGGATTCCGGAA
GAAACATTTGAAACTGGAATTGTCAAAACCATCGATTGGTATTTGAAAAAATATAAGAATTAA

Upstream 100 bases:

>100_bases
TTTTATCGGTTTGGAAACTTTAAAAAAACATGCGACACTGAATCAAAAAAATCAGTACGGTGTCTATCTGCAAGAAATTG
TGAATTCGGTGATGAGATAA

Downstream 100 bases:

>100_bases
TACTCTCTGAAAAGAAGAATGGAATGAAGTAGTTTTCAGTATGAAAAAAAACTCAAAAATCTATGTCGCAGGGCACAATG
GACTCGTAGGCTCCGCATTG

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MEFKNVLVTGSAGFIGSNFVPYFLETHPHVHVVSLDKITYAGNLDNLSSIISEKRHTFVNGDICDAELVGSLFETYKFDL
VVHFAAESHVDNSIINPKIFLETNVIGTFQLLQTAYSTWFSAPFQRKPGFEAAKFIHISTDEVFGSLGESGFFTEDSPYQ
PNSPYSASKASSDHIVRSYYHTYGLPMIITNCSNNYGPKQHNEKLIPTIIRNALEDKNIPIYGTGMNIRDWLFVMDHVKG
IEKVILYGKLGETYNIGGNNELTNNQVVNVICNHLDKVIPRGNGKSYSELIMYVSDRPGHDKRYAIDATKMKKDLNWIPE
ETFETGIVKTIDWYLKKYKN

Sequences:

>Translated_340_residues
MEFKNVLVTGSAGFIGSNFVPYFLETHPHVHVVSLDKITYAGNLDNLSSIISEKRHTFVNGDICDAELVGSLFETYKFDL
VVHFAAESHVDNSIINPKIFLETNVIGTFQLLQTAYSTWFSAPFQRKPGFEAAKFIHISTDEVFGSLGESGFFTEDSPYQ
PNSPYSASKASSDHIVRSYYHTYGLPMIITNCSNNYGPKQHNEKLIPTIIRNALEDKNIPIYGTGMNIRDWLFVMDHVKG
IEKVILYGKLGETYNIGGNNELTNNQVVNVICNHLDKVIPRGNGKSYSELIMYVSDRPGHDKRYAIDATKMKKDLNWIPE
ETFETGIVKTIDWYLKKYKN
>Mature_340_residues
MEFKNVLVTGSAGFIGSNFVPYFLETHPHVHVVSLDKITYAGNLDNLSSIISEKRHTFVNGDICDAELVGSLFETYKFDL
VVHFAAESHVDNSIINPKIFLETNVIGTFQLLQTAYSTWFSAPFQRKPGFEAAKFIHISTDEVFGSLGESGFFTEDSPYQ
PNSPYSASKASSDHIVRSYYHTYGLPMIITNCSNNYGPKQHNEKLIPTIIRNALEDKNIPIYGTGMNIRDWLFVMDHVKG
IEKVILYGKLGETYNIGGNNELTNNQVVNVICNHLDKVIPRGNGKSYSELIMYVSDRPGHDKRYAIDATKMKKDLNWIPE
ETFETGIVKTIDWYLKKYKN

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=339, Percent_Identity=39.5280235988201, Blast_Score=239, Evalue=4e-63,
Organism=Homo sapiens, GI42516563, Length=341, Percent_Identity=23.7536656891496, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1788353, Length=343, Percent_Identity=52.1865889212828, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI48994969, Length=336, Percent_Identity=51.4880952380952, Blast_Score=353, Evalue=1e-98,
Organism=Escherichia coli, GI1786974, Length=357, Percent_Identity=25.7703081232493, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788366, Length=360, Percent_Identity=24.1666666666667, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17568069, Length=334, Percent_Identity=34.4311377245509, Blast_Score=204, Evalue=4e-53,
Organism=Caenorhabditis elegans, GI115532424, Length=341, Percent_Identity=33.4310850439883, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17539532, Length=338, Percent_Identity=24.5562130177515, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI71982035, Length=367, Percent_Identity=23.9782016348774, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71982038, Length=369, Percent_Identity=23.8482384823848, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI133901786, Length=334, Percent_Identity=21.8562874251497, Blast_Score=65, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17539422, Length=334, Percent_Identity=21.8562874251497, Blast_Score=64, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI133901788, Length=334, Percent_Identity=21.8562874251497, Blast_Score=64, Evalue=1e-10,
Organism=Caenorhabditis elegans, GI133901790, Length=334, Percent_Identity=21.8562874251497, Blast_Score=64, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6319493, Length=360, Percent_Identity=24.4444444444444, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21356223, Length=338, Percent_Identity=24.2603550295858, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19923002, Length=366, Percent_Identity=25.9562841530055, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24158427, Length=343, Percent_Identity=23.3236151603499, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 38490; Mature: 38490

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFKNVLVTGSAGFIGSNFVPYFLETHPHVHVVSLDKITYAGNLDNLSSIISEKRHTFVN
CCCCCEEEECCCCCCCCCCCCHHCCCCCCEEEEEECCEEECCCHHHHHHHHHHHHHEEEC
GDICDAELVGSLFETYKFDLVVHFAAESHVDNSIINPKIFLETNVIGTFQLLQTAYSTWF
CCCCCHHHHHHHHHHHEEEEEEEEEHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHH
SAPFQRKPGFEAAKFIHISTDEVFGSLGESGFFTEDSPYQPNSPYSASKASSDHIVRSYY
CCCCCCCCCCCCEEEEEEEHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
HTYGLPMIITNCSNNYGPKQHNEKLIPTIIRNALEDKNIPIYGTGMNIRDWLFVMDHVKG
HHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHH
IEKVILYGKLGETYNIGGNNELTNNQVVNVICNHLDKVIPRGNGKSYSELIMYVSDRPGH
HHHEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHEEEEECCCCCC
DKRYAIDATKMKKDLNWIPEETFETGIVKTIDWYLKKYKN
CCEEEEEHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEFKNVLVTGSAGFIGSNFVPYFLETHPHVHVVSLDKITYAGNLDNLSSIISEKRHTFVN
CCCCCEEEECCCCCCCCCCCCHHCCCCCCEEEEEECCEEECCCHHHHHHHHHHHHHEEEC
GDICDAELVGSLFETYKFDLVVHFAAESHVDNSIINPKIFLETNVIGTFQLLQTAYSTWF
CCCCCHHHHHHHHHHHEEEEEEEEEHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHH
SAPFQRKPGFEAAKFIHISTDEVFGSLGESGFFTEDSPYQPNSPYSASKASSDHIVRSYY
CCCCCCCCCCCCEEEEEEEHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
HTYGLPMIITNCSNNYGPKQHNEKLIPTIIRNALEDKNIPIYGTGMNIRDWLFVMDHVKG
HHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHH
IEKVILYGKLGETYNIGGNNELTNNQVVNVICNHLDKVIPRGNGKSYSELIMYVSDRPGH
HHHEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHEEEEECCCCCC
DKRYAIDATKMKKDLNWIPEETFETGIVKTIDWYLKKYKN
CCEEEEEHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253667 [H]