Click here to switch to the map view.

The map label for this gene is fcl [H]

Identifier: 183221420

GI number: 183221420

Start: 2119255

End: 2120211

Strand: Reverse

Name: fcl [H]

Synonym: LEPBI_I2038

Alternate gene names: 183221420

Gene position: 2120211-2119255 (Counterclockwise)

Preceding gene: 183221421

Following gene: 183221419

Centisome position: 58.9

GC content: 40.23

Gene sequence:

>957_bases
ATGAAAAAAAACTCAAAAATCTATGTCGCAGGGCACAATGGACTCGTAGGCTCCGCATTGGTCCGAGTCTTAAAACAACA
AGGATTTACCAATGTGATTGGTAGAACCAGAAGTGAACTTGATTTAACGAACCAATTAGAAGTGAATCAATTCTTTGAAA
AAGAACGCCCTGAATATGTTTTCCTTGCCGCAGCCAAAGTCGGTGGAATCCATGCAAATAATTCCTACCCCGCTGAGTTT
ATATTCTCAAACTTACAAATCCAAAATAATATTATTGATGCAACCTATCGATATCAGGGCAAAAAGCTATGTTTTTTAGG
TTCCTCTTGCATTTACCCTAAATTTGCAAAGCAACCTATGGACGAAGGTCAACTGTTAGATGGAAAACTTGAACCGACAA
ATGAGCCATATGCCGTGGCAAAAATTGCGGGAATTGTGATGTGCCAAAGTTACAATAGACAATACGGCACCGAGTTTTTT
TCAGTGATGCCAACAAACCTTTATGGACCCGGTGATAATTATCATCCGCAAAATTCGCACGTCTTACCTGCATTACTTCG
TAGGTTTCATGAGGCAAAGGTGAATGGCCTTCCTGAAGTAGTCATTTGGGGTACGGGCAATCCACTTCGCGAGTTTTTGT
ACTCGGATGATATGGCAAGGGCATGTGTTTTTTTGATGCAAAACTACAGTGAGTTTCAAGAATCTCGTGGTGGAGAACAT
GTAAATGTTGGATCTGGGATTGAAGTGAGCATTCGGGAACTTGCCGAAACTCTCAAAGATGTAGTGGGTTATCATGGTAA
ACTTACGTTTGATTTAACAAAACCAGACGGAACGCCTAGAAAGTTATTGGATGTTTCTAAATTACATAGAATGGGTTGGA
AACACGAAGTGGAGTTGAGAGAAGGAATTCGATTGGCATATGATGATTTCCTGCAAAATGGTGGAATTGAAAGGTAA

Upstream 100 bases:

>100_bases
ACATTTGAAACTGGAATTGTCAAAACCATCGATTGGTATTTGAAAAAATATAAGAATTAATACTCTCTGAAAAGAAGAAT
GGAATGAAGTAGTTTTCAGT

Downstream 100 bases:

>100_bases
GGGAATAAGAGATGAGTGTAAAATCGATATTAGTAACTGGTGGAACGGGATCTTTTGGAAAAAGGTTTATAACAAGATTG
ATCAAAGCATATCCAGATGT

Product: GDP-L-fucose synthetase

Products: NA

Alternate protein names: GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MKKNSKIYVAGHNGLVGSALVRVLKQQGFTNVIGRTRSELDLTNQLEVNQFFEKERPEYVFLAAAKVGGIHANNSYPAEF
IFSNLQIQNNIIDATYRYQGKKLCFLGSSCIYPKFAKQPMDEGQLLDGKLEPTNEPYAVAKIAGIVMCQSYNRQYGTEFF
SVMPTNLYGPGDNYHPQNSHVLPALLRRFHEAKVNGLPEVVIWGTGNPLREFLYSDDMARACVFLMQNYSEFQESRGGEH
VNVGSGIEVSIRELAETLKDVVGYHGKLTFDLTKPDGTPRKLLDVSKLHRMGWKHEVELREGIRLAYDDFLQNGGIER

Sequences:

>Translated_318_residues
MKKNSKIYVAGHNGLVGSALVRVLKQQGFTNVIGRTRSELDLTNQLEVNQFFEKERPEYVFLAAAKVGGIHANNSYPAEF
IFSNLQIQNNIIDATYRYQGKKLCFLGSSCIYPKFAKQPMDEGQLLDGKLEPTNEPYAVAKIAGIVMCQSYNRQYGTEFF
SVMPTNLYGPGDNYHPQNSHVLPALLRRFHEAKVNGLPEVVIWGTGNPLREFLYSDDMARACVFLMQNYSEFQESRGGEH
VNVGSGIEVSIRELAETLKDVVGYHGKLTFDLTKPDGTPRKLLDVSKLHRMGWKHEVELREGIRLAYDDFLQNGGIER
>Mature_318_residues
MKKNSKIYVAGHNGLVGSALVRVLKQQGFTNVIGRTRSELDLTNQLEVNQFFEKERPEYVFLAAAKVGGIHANNSYPAEF
IFSNLQIQNNIIDATYRYQGKKLCFLGSSCIYPKFAKQPMDEGQLLDGKLEPTNEPYAVAKIAGIVMCQSYNRQYGTEFF
SVMPTNLYGPGDNYHPQNSHVLPALLRRFHEAKVNGLPEVVIWGTGNPLREFLYSDDMARACVFLMQNYSEFQESRGGEH
VNVGSGIEVSIRELAETLKDVVGYHGKLTFDLTKPDGTPRKLLDVSKLHRMGWKHEVELREGIRLAYDDFLQNGGIER

Specific function: Two step NADP-dependent conversion of GDP-4-dehydro-6- deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=286, Percent_Identity=34.6153846153846, Blast_Score=182, Evalue=4e-46,
Organism=Homo sapiens, GI7657641, Length=282, Percent_Identity=23.758865248227, Blast_Score=78, Evalue=9e-15,
Organism=Escherichia coli, GI1788365, Length=311, Percent_Identity=57.556270096463, Blast_Score=391, Evalue=1e-110,
Organism=Escherichia coli, GI1788353, Length=355, Percent_Identity=24.7887323943662, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI48994969, Length=362, Percent_Identity=24.3093922651934, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17554428, Length=288, Percent_Identity=30.9027777777778, Blast_Score=152, Evalue=2e-37,
Organism=Drosophila melanogaster, GI19922778, Length=291, Percent_Identity=32.6460481099656, Blast_Score=159, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.271 [H]

Molecular weight: Translated: 35904; Mature: 35904

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNSKIYVAGHNGLVGSALVRVLKQQGFTNVIGRTRSELDLTNQLEVNQFFEKERPEYV
CCCCCEEEEEECCCCHHHHHHHHHHHCCCHHHHHCCHHHCCCCCCHHHHHHHHHCCCCEE
FLAAAKVGGIHANNSYPAEFIFSNLQIQNNIIDATYRYQGKKLCFLGSSCIYPKFAKQPM
EEEEEEECCEECCCCCCHHHHHHCCEEECCEECCEEEECCCEEEEECCCCCCCHHHCCCC
DEGQLLDGKLEPTNEPYAVAKIAGIVMCQSYNRQYGTEFFSVMPTNLYGPGDNYHPQNSH
CCCCEECCCCCCCCCCHHHHHHHHHHHHHHCCHHHCCHHHHHCCCCCCCCCCCCCCCCCC
VLPALLRRFHEAKVNGLPEVVIWGTGNPLREFLYSDDMARACVFLMQNYSEFQESRGGEH
HHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCE
VNVGSGIEVSIRELAETLKDVVGYHGKLTFDLTKPDGTPRKLLDVSKLHRMGWKHEVELR
EECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEHHH
EGIRLAYDDFLQNGGIER
HCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKKNSKIYVAGHNGLVGSALVRVLKQQGFTNVIGRTRSELDLTNQLEVNQFFEKERPEYV
CCCCCEEEEEECCCCHHHHHHHHHHHCCCHHHHHCCHHHCCCCCCHHHHHHHHHCCCCEE
FLAAAKVGGIHANNSYPAEFIFSNLQIQNNIIDATYRYQGKKLCFLGSSCIYPKFAKQPM
EEEEEEECCEECCCCCCHHHHHHCCEEECCEECCEEEECCCEEEEECCCCCCCHHHCCCC
DEGQLLDGKLEPTNEPYAVAKIAGIVMCQSYNRQYGTEFFSVMPTNLYGPGDNYHPQNSH
CCCCEECCCCCCCCCCHHHHHHHHHHHHHHCCHHHCCHHHHHCCCCCCCCCCCCCCCCCC
VLPALLRRFHEAKVNGLPEVVIWGTGNPLREFLYSDDMARACVFLMQNYSEFQESRGGEH
HHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCE
VNVGSGIEVSIRELAETLKDVVGYHGKLTFDLTKPDGTPRKLLDVSKLHRMGWKHEVELR
EECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEHHH
EGIRLAYDDFLQNGGIER
HCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7815923; 8759852; 9097040; 9278503; 9473059; 9862812 [H]