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The map label for this gene is proA

Identifier: 183221164

GI number: 183221164

Start: 1855749

End: 1857014

Strand: Direct

Name: proA

Synonym: LEPBI_I1778

Alternate gene names: 183221164

Gene position: 1855749-1857014 (Clockwise)

Preceding gene: 183221163

Following gene: 183221165

Centisome position: 51.55

GC content: 41.94

Gene sequence:

>1266_bases
ATGACATTCGAAGCAACAGAATATGCAAAGTCCATTGCAACAAAGGCAAAAGAAGCAAGTCGTGCATTAAAAAGTCTCAC
AACCCTACAAAAGAATTCTGTTTTAAAACGAGTGGAAACACTGCTTTTGGAAAATGAAACGGCAATCATCAAAGAAAACC
AAATCGATTTACAAAATGGAATTCAAAAAGGACTTTCATCCGCCATGATGGACCGTCTGTTATTAGATTCCAAACGAATC
CAATCGATGGCAAAGAGCATAGAAGAGATTCGAAACCTTCCCGATCCCGTGGGTGAAGTTGTACGCGGGACCATCCTTCC
GAATGGACTCGAACTTCTTACGAAACGTGTGCCCATTGGTGTGGTCATGACCATTTTTGAATCTAGACCCAATGTGATTG
TCGACATTGCATCTTTATCCTTTAAATCGGGGAACGCTTGTATCCTACGTGGTGGGAGTGAAGCATATCACTCCAATTTG
ATTTTGTCATCCTTATTCCATCAAGCAATTGGTGAGTCGAACCTTCCCAGTGTCACAAAAGATGTGGTGAGTTTTGTGGA
AAATACGGATCGCGAAGCGATGGTTCCGTTTTTCCAATTGGATGATCTCATTGATGTGATTGTTCCTAGAGGGGGCGAAG
CCCTCATCCGATTTGTATCAGAGAATAGTAAAATCCCTGTCATCAAACACGATAAAGGTGTCACGAATTTATATCTATCC
AATAAAGCCAAGGAAGACATTGTCCTTCCTATTCTAATCAATTCTAAAGTCCAACGCCCAGGGGTTTGTAATGCACTTGA
GAATTTATTCATTCACAAAGACTACCCCCATATTCAAACCTTACTGAGTGATTTGCAAAAGGCTGGTGTTCAGGTTTTAG
GAGATCAGTCGACCCAATCCATTTTTCCGAACCTACCGCTTGCAACAGAAGCAGATTTTTACACAGAGTTTTTAGATACA
AGGCTCAGTGTCAAAATCGTAAAATCAGTCGAAGATGCCATGCAAAACATCCAAAACTACAGTTCAGGTCATACGGAATG
CATTCTTTCAGAAGATGAATCAGAAATCCAAACCTTTCGCCAAGGACTCGATAGTGCGGCTATCTTTGTCAATTGTTCCA
CAAGATTCCATGATGGAGGCGAATTTGGCCTAGGAGCAGAAGTTGGAATTTCCACAGGAAAACTCCATGTGAGGGGGCCC
ATGGGACTTATCCACCTAACTACGACTACCACCTATGTTACAGGAAAAGGACAAGTCCGAGGATAA

Upstream 100 bases:

>100_bases
ATCGTTTGGGACCTTGGTTGCCAATGGAAGTTTTCCACATAGAATTCCTTCCGCTTCTGAAATCCAATCACACTTTTTTT
CCTATCAGGTGGAGTAAACT

Downstream 100 bases:

>100_bases
AATGGATGTGATCTTGTTTGGTGGGAGTTTTAACCCACCGCACATAGGGCACAGACATGTGATCACTACCATTCGGAACC
AATTCCCAAAATCAAAACTT

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MTFEATEYAKSIATKAKEASRALKSLTTLQKNSVLKRVETLLLENETAIIKENQIDLQNGIQKGLSSAMMDRLLLDSKRI
QSMAKSIEEIRNLPDPVGEVVRGTILPNGLELLTKRVPIGVVMTIFESRPNVIVDIASLSFKSGNACILRGGSEAYHSNL
ILSSLFHQAIGESNLPSVTKDVVSFVENTDREAMVPFFQLDDLIDVIVPRGGEALIRFVSENSKIPVIKHDKGVTNLYLS
NKAKEDIVLPILINSKVQRPGVCNALENLFIHKDYPHIQTLLSDLQKAGVQVLGDQSTQSIFPNLPLATEADFYTEFLDT
RLSVKIVKSVEDAMQNIQNYSSGHTECILSEDESEIQTFRQGLDSAAIFVNCSTRFHDGGEFGLGAEVGISTGKLHVRGP
MGLIHLTTTTTYVTGKGQVRG

Sequences:

>Translated_421_residues
MTFEATEYAKSIATKAKEASRALKSLTTLQKNSVLKRVETLLLENETAIIKENQIDLQNGIQKGLSSAMMDRLLLDSKRI
QSMAKSIEEIRNLPDPVGEVVRGTILPNGLELLTKRVPIGVVMTIFESRPNVIVDIASLSFKSGNACILRGGSEAYHSNL
ILSSLFHQAIGESNLPSVTKDVVSFVENTDREAMVPFFQLDDLIDVIVPRGGEALIRFVSENSKIPVIKHDKGVTNLYLS
NKAKEDIVLPILINSKVQRPGVCNALENLFIHKDYPHIQTLLSDLQKAGVQVLGDQSTQSIFPNLPLATEADFYTEFLDT
RLSVKIVKSVEDAMQNIQNYSSGHTECILSEDESEIQTFRQGLDSAAIFVNCSTRFHDGGEFGLGAEVGISTGKLHVRGP
MGLIHLTTTTTYVTGKGQVRG
>Mature_420_residues
TFEATEYAKSIATKAKEASRALKSLTTLQKNSVLKRVETLLLENETAIIKENQIDLQNGIQKGLSSAMMDRLLLDSKRIQ
SMAKSIEEIRNLPDPVGEVVRGTILPNGLELLTKRVPIGVVMTIFESRPNVIVDIASLSFKSGNACILRGGSEAYHSNLI
LSSLFHQAIGESNLPSVTKDVVSFVENTDREAMVPFFQLDDLIDVIVPRGGEALIRFVSENSKIPVIKHDKGVTNLYLSN
KAKEDIVLPILINSKVQRPGVCNALENLFIHKDYPHIQTLLSDLQKAGVQVLGDQSTQSIFPNLPLATEADFYTEFLDTR
LSVKIVKSVEDAMQNIQNYSSGHTECILSEDESEIQTFRQGLDSAAIFVNCSTRFHDGGEFGLGAEVGISTGKLHVRGPM
GLIHLTTTTTYVTGKGQVRG

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family

Homologues:

Organism=Homo sapiens, GI21361368, Length=409, Percent_Identity=37.1638141809291, Blast_Score=256, Evalue=3e-68,
Organism=Homo sapiens, GI62912457, Length=409, Percent_Identity=37.1638141809291, Blast_Score=256, Evalue=4e-68,
Organism=Escherichia coli, GI1786438, Length=412, Percent_Identity=38.3495145631068, Blast_Score=291, Evalue=6e-80,
Organism=Caenorhabditis elegans, GI17569969, Length=413, Percent_Identity=37.046004842615, Blast_Score=271, Evalue=6e-73,
Organism=Caenorhabditis elegans, GI17569967, Length=415, Percent_Identity=36.8674698795181, Blast_Score=271, Evalue=6e-73,
Organism=Saccharomyces cerevisiae, GI6324899, Length=433, Percent_Identity=34.4110854503464, Blast_Score=254, Evalue=2e-68,
Organism=Drosophila melanogaster, GI21357643, Length=424, Percent_Identity=33.9622641509434, Blast_Score=236, Evalue=1e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROA_LEPBA (B0S9A5)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001962810.1
- ProteinModelPortal:   B0S9A5
- SMR:   B0S9A5
- GeneID:   6387375
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_1725
- HOGENOM:   HBG318080
- OMA:   QYPAACN
- ProtClustDB:   PRK00197
- BioCyc:   LBIF355278:LBF_1725-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00412
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR012134
- Gene3D:   G3DSA:3.40.309.10
- Gene3D:   G3DSA:3.40.605.10
- PANTHER:   PTHR11063:SF1
- PIRSF:   PIRSF000151
- TIGRFAMs:   TIGR00407

Pfam domain/function: PF00171 Aldedh; SSF53720 Aldehyde_DH/Histidinol_DH

EC number: =1.2.1.41

Molecular weight: Translated: 46199; Mature: 46067

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFEATEYAKSIATKAKEASRALKSLTTLQKNSVLKRVETLLLENETAIIKENQIDLQNG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHH
IQKGLSSAMMDRLLLDSKRIQSMAKSIEEIRNLPDPVGEVVRGTILPNGLELLTKRVPIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHCCHH
VVMTIFESRPNVIVDIASLSFKSGNACILRGGSEAYHSNLILSSLFHQAIGESNLPSVTK
HHHHHHHCCCCEEEEEEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHH
DVVSFVENTDREAMVPFFQLDDLIDVIVPRGGEALIRFVSENSKIPVIKHDKGVTNLYLS
HHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEECCCCCEEEEEC
NKAKEDIVLPILINSKVQRPGVCNALENLFIHKDYPHIQTLLSDLQKAGVQVLGDQSTQS
CCCCCCEEEEEEECCCCCCCCHHHHHHHHEECCCCHHHHHHHHHHHHCCCEEECCCCHHH
IFPNLPLATEADFYTEFLDTRLSVKIVKSVEDAMQNIQNYSSGHTECILSEDESEIQTFR
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHH
QGLDSAAIFVNCSTRFHDGGEFGLGAEVGISTGKLHVRGPMGLIHLTTTTTYVTGKGQVR
HCCCCEEEEEEECCCCCCCCCCCCCCEECCCCCEEEEECCCEEEEEEEEEEEEECCCCCC
G
C
>Mature Secondary Structure 
TFEATEYAKSIATKAKEASRALKSLTTLQKNSVLKRVETLLLENETAIIKENQIDLQNG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHH
IQKGLSSAMMDRLLLDSKRIQSMAKSIEEIRNLPDPVGEVVRGTILPNGLELLTKRVPIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHCCHH
VVMTIFESRPNVIVDIASLSFKSGNACILRGGSEAYHSNLILSSLFHQAIGESNLPSVTK
HHHHHHHCCCCEEEEEEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHH
DVVSFVENTDREAMVPFFQLDDLIDVIVPRGGEALIRFVSENSKIPVIKHDKGVTNLYLS
HHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEECCCCCEEEEEC
NKAKEDIVLPILINSKVQRPGVCNALENLFIHKDYPHIQTLLSDLQKAGVQVLGDQSTQS
CCCCCCEEEEEEECCCCCCCCHHHHHHHHEECCCCHHHHHHHHHHHHCCCEEECCCCHHH
IFPNLPLATEADFYTEFLDTRLSVKIVKSVEDAMQNIQNYSSGHTECILSEDESEIQTFR
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHH
QGLDSAAIFVNCSTRFHDGGEFGLGAEVGISTGKLHVRGPMGLIHLTTTTTYVTGKGQVR
HCCCCEEEEEEECCCCCCCCCCCCCCEECCCCCEEEEECCCEEEEEEEEEEEEECCCCCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA