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The map label for this gene is gspF [H]

Identifier: 183221064

GI number: 183221064

Start: 1749506

End: 1750732

Strand: Direct

Name: gspF [H]

Synonym: LEPBI_I1677

Alternate gene names: 183221064

Gene position: 1749506-1750732 (Clockwise)

Preceding gene: 183221063

Following gene: 183221065

Centisome position: 48.6

GC content: 38.47

Gene sequence:

>1227_bases
ATGCCACTTTATACCTACGTTGCCTTTAATAAAAAGGGAAAAGAAGAAAAAAACATCATTGATGCTGTGAATTTGCAAGC
AGCACGGAACAAACTAAAAGCGAAAGGTTTATACGTAAGATCCATTCAAGAAGATAGAGAAAAAGAAGAAAGGGAATTGT
TTCCTTTTTTATCAAAACTTTTGTATCGAATTCCAAGAAAGGATGTTGGTCTTTTTTGTAAACAATTAGGGACTCTCATT
GGAGCTGGAATCCCACTGGATAAGTGTTTACTTTCCATCATTGACCAAGTGGAGAATATTTACTTCAAAAAAGTTCTGAT
TGAAATGCGGGCAGACATTACAGAAGGAATGAGTCTTTCTGAATCCATGAAAAAACACAAAACTGTGTTTCCCGACCAAT
ACCCAAGTTTGATATCCGTCGGTGAATCGACAGGAAATTACGAAAACACCTTGCACCGGTTAGCGGAGCTGGAAGAAAAA
TCATCGGAACTAAAATCAAAGGTGCAGGTGGCAATGATTTACCCCATGATTATGGGTTTATTGTCTCTTGGTGTATCCAT
CTTTTTACTTGTGGTGGTGATCCCACAAATTGAACAGTTGTTTGCATCCTTTGATGCCAAACTCCCACTCCTCACACGAG
CTGTGATTTTTTTGTCCTATGTTCTCACCAATTATTGGTATTTTATTTTGGCATTCATCGCTGGTAGTTTTTTAAGTTTT
TTGAAATGGAAAAGTTCAGAGGATGGAAAAAAAGTTTGGGATACATTTTTACTAAGACTCCCTGTGATCGGGACATTACT
TCGTAAAATTTTAGTTTCCAATTTTGCAAGAAACCTGTCCATTTTACTTCTGAACCGTGTGCCTCTCATTGTTTCATTAA
ATATTGTTTCGGATGTTGTGGGACATACGGTTTTTAAGGAAGAGATTGATGCAGCGATCATCAAAATCAAGGAAGGGGGA
AAACTTTCCGACTCCTTACAAGGAAGCCAAGTCCTTCCGCAGATGGTCCTTGGGATGCTCAGTGCCGGGGAAGCCTCCGA
TAAAGTCCCAGAAATGATGAATAAACTCTCAGAAATCTACGAATCTGAGGTGGATACAGCAATAAAATCTTTGACCCAAT
CATTAGAACCTATGATGATCATAGTAATGGGTGGAATTATTTTTACCATTATGGCGGCAATTATGACGCCAATGTACAAA
CTAACTCAAGAAATCCAGGGGATGTAG

Upstream 100 bases:

>100_bases
GCCGTTAAAAATGGACTATCTGTCCTTCAAGAATATGGTAAGTACAAAGTGATCGAGGGAGTGACAACTCCTGAAGAAGT
CCTTCGGGTATCTTAAGTTT

Downstream 100 bases:

>100_bases
AGTTCATGAAAATCAAAGGAAACAACAGAAAGATCCGTGAGGGATTAACTCTAATTGAGATTACTGTCGTGATGCTCATT
TTAGGATCACTTATGGCCAT

Product: general secretion pathway protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MPLYTYVAFNKKGKEEKNIIDAVNLQAARNKLKAKGLYVRSIQEDREKEERELFPFLSKLLYRIPRKDVGLFCKQLGTLI
GAGIPLDKCLLSIIDQVENIYFKKVLIEMRADITEGMSLSESMKKHKTVFPDQYPSLISVGESTGNYENTLHRLAELEEK
SSELKSKVQVAMIYPMIMGLLSLGVSIFLLVVVIPQIEQLFASFDAKLPLLTRAVIFLSYVLTNYWYFILAFIAGSFLSF
LKWKSSEDGKKVWDTFLLRLPVIGTLLRKILVSNFARNLSILLLNRVPLIVSLNIVSDVVGHTVFKEEIDAAIIKIKEGG
KLSDSLQGSQVLPQMVLGMLSAGEASDKVPEMMNKLSEIYESEVDTAIKSLTQSLEPMMIIVMGGIIFTIMAAIMTPMYK
LTQEIQGM

Sequences:

>Translated_408_residues
MPLYTYVAFNKKGKEEKNIIDAVNLQAARNKLKAKGLYVRSIQEDREKEERELFPFLSKLLYRIPRKDVGLFCKQLGTLI
GAGIPLDKCLLSIIDQVENIYFKKVLIEMRADITEGMSLSESMKKHKTVFPDQYPSLISVGESTGNYENTLHRLAELEEK
SSELKSKVQVAMIYPMIMGLLSLGVSIFLLVVVIPQIEQLFASFDAKLPLLTRAVIFLSYVLTNYWYFILAFIAGSFLSF
LKWKSSEDGKKVWDTFLLRLPVIGTLLRKILVSNFARNLSILLLNRVPLIVSLNIVSDVVGHTVFKEEIDAAIIKIKEGG
KLSDSLQGSQVLPQMVLGMLSAGEASDKVPEMMNKLSEIYESEVDTAIKSLTQSLEPMMIIVMGGIIFTIMAAIMTPMYK
LTQEIQGM
>Mature_407_residues
PLYTYVAFNKKGKEEKNIIDAVNLQAARNKLKAKGLYVRSIQEDREKEERELFPFLSKLLYRIPRKDVGLFCKQLGTLIG
AGIPLDKCLLSIIDQVENIYFKKVLIEMRADITEGMSLSESMKKHKTVFPDQYPSLISVGESTGNYENTLHRLAELEEKS
SELKSKVQVAMIYPMIMGLLSLGVSIFLLVVVIPQIEQLFASFDAKLPLLTRAVIFLSYVLTNYWYFILAFIAGSFLSFL
KWKSSEDGKKVWDTFLLRLPVIGTLLRKILVSNFARNLSILLLNRVPLIVSLNIVSDVVGHTVFKEEIDAAIIKIKEGGK
LSDSLQGSQVLPQMVLGMLSAGEASDKVPEMMNKLSEIYESEVDTAIKSLTQSLEPMMIIVMGGIIFTIMAAIMTPMYKL
TQEIQGM

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins [H]

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1789724, Length=402, Percent_Identity=28.6069651741293, Blast_Score=185, Evalue=6e-48,
Organism=Escherichia coli, GI1786295, Length=344, Percent_Identity=23.2558139534884, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR011850
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 45898; Mature: 45767

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: PS00874 T2SP_F

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLYTYVAFNKKGKEEKNIIDAVNLQAARNKLKAKGLYVRSIQEDREKEERELFPFLSKL
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LYRIPRKDVGLFCKQLGTLIGAGIPLDKCLLSIIDQVENIYFKKVLIEMRADITEGMSLS
HHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
ESMKKHKTVFPDQYPSLISVGESTGNYENTLHRLAELEEKSSELKSKVQVAMIYPMIMGL
HHHHHHCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSLGVSIFLLVVVIPQIEQLFASFDAKLPLLTRAVIFLSYVLTNYWYFILAFIAGSFLSF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKWKSSEDGKKVWDTFLLRLPVIGTLLRKILVSNFARNLSILLLNRVPLIVSLNIVSDVV
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
GHTVFKEEIDAAIIKIKEGGKLSDSLQGSQVLPQMVLGMLSAGEASDKVPEMMNKLSEIY
HHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
ESEVDTAIKSLTQSLEPMMIIVMGGIIFTIMAAIMTPMYKLTQEIQGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PLYTYVAFNKKGKEEKNIIDAVNLQAARNKLKAKGLYVRSIQEDREKEERELFPFLSKL
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LYRIPRKDVGLFCKQLGTLIGAGIPLDKCLLSIIDQVENIYFKKVLIEMRADITEGMSLS
HHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
ESMKKHKTVFPDQYPSLISVGESTGNYENTLHRLAELEEKSSELKSKVQVAMIYPMIMGL
HHHHHHCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSLGVSIFLLVVVIPQIEQLFASFDAKLPLLTRAVIFLSYVLTNYWYFILAFIAGSFLSF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKWKSSEDGKKVWDTFLLRLPVIGTLLRKILVSNFARNLSILLLNRVPLIVSLNIVSDVV
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
GHTVFKEEIDAAIIKIKEGGKLSDSLQGSQVLPQMVLGMLSAGEASDKVPEMMNKLSEIY
HHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
ESEVDTAIKSLTQSLEPMMIIVMGGIIFTIMAAIMTPMYKLTQEIQGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1640836 [H]