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The map label for this gene is yogA [H]

Identifier: 183220931

GI number: 183220931

Start: 1609476

End: 1610477

Strand: Reverse

Name: yogA [H]

Synonym: LEPBI_I1544

Alternate gene names: 183220931

Gene position: 1610477-1609476 (Counterclockwise)

Preceding gene: 183220932

Following gene: 183220930

Centisome position: 44.74

GC content: 39.72

Gene sequence:

>1002_bases
ATGAAAGCATTCACCATCAACCGATACAATAAACACCAAAACCTGCAACTCACAGAAGTATCTGATCCCATCTTAAACGA
TCATGATGTTCTTGTACAAATTCATGCGGCAGGTATTAATCTTTTGGATTCCAAACTAAAGAGTGGCGAATTCAAGCTGA
TTTTACCTTATCAATTGCCACTCATTTTGGGTCATGATTTTGCAGGAATCATTTTGAAAGTCGGTCCCAAAGTTCAGAAA
TTTAAGATAGGAGAAGAAGTGTTTGGACGAGTGAGAGACTTTAGAATTGGAACTTTTGCAGAACAAATTGCAGTCAGTGA
AGATGATATCGCAAAAAAACCAAAACATTTGACGATGGAAGAAGCTGCTTCCATTCCACTTGTTGGTCTTACTTCTTGGC
AGGCATTGGTAGAAAATGCAAACATTCAAAAAGGGCAAAAGGTTTTCATCCAAGCAGGTTCTGGTGGGGTTGGAACGTTT
GCCATCCAATTAGCAAAGGTTTTAGGAGCAGAGGTCGCCACAACAACGAGTCATTCTAATTTTGAACTTGTAAAACGTTT
AGGCGCCGATACCATCATTGATTACAAAACAACTGATTTTGAATCCATTCTGAAGGACTATGATGTCGTGATCCATAGCC
AGGATGGAAAGACTCTCCATAAATCACTGCGAATCTTAAAACCTGGTGGGAAACTCATTTCGATCTCAGGACCTCCTGAT
ATCAATTTTGCAAAGGAAATGAAATTTCCATGGTTCTTACAATTTGTGATCCGAATGCTCAGCCTTTCAGCAAACAAAAA
AGCAAAGGAACGGAATGTTCAATATTCCTTCCTTTTTATGAAAGCTAACGGAAGCCAATTGAACCAAATCTCAAACCTAA
TCAATGAAGGAAGGATTCAGCCGGTGATCGACAAAATTTACCCGTTTGAAGCACTCAACGATGCCTTGGCCTATGTGGAA
AGTGGACGTGCCAAAGGGAAAGTTGTGGTTAAAATGCTGTAG

Upstream 100 bases:

>100_bases
TATCCTTTTTATGCAACAATATGTTTATAAAGTTTTTGAAAATTCAATTCTAAAACAAGTTCATAAGGCATTCTAAAAAG
GAATCAATCAGGTAAATTAA

Downstream 100 bases:

>100_bases
GATGAACTGCATCATGAAAGGAATTTCTTAGAATTTTGAATCCAAACAAACGGAACACATTTTACGCTTATTTCAAAAAT
CTCACAAATATCTATAGAAC

Product: zinc-containing alcohol dehydrogenase superfamily protein

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MKAFTINRYNKHQNLQLTEVSDPILNDHDVLVQIHAAGINLLDSKLKSGEFKLILPYQLPLILGHDFAGIILKVGPKVQK
FKIGEEVFGRVRDFRIGTFAEQIAVSEDDIAKKPKHLTMEEAASIPLVGLTSWQALVENANIQKGQKVFIQAGSGGVGTF
AIQLAKVLGAEVATTTSHSNFELVKRLGADTIIDYKTTDFESILKDYDVVIHSQDGKTLHKSLRILKPGGKLISISGPPD
INFAKEMKFPWFLQFVIRMLSLSANKKAKERNVQYSFLFMKANGSQLNQISNLINEGRIQPVIDKIYPFEALNDALAYVE
SGRAKGKVVVKML

Sequences:

>Translated_333_residues
MKAFTINRYNKHQNLQLTEVSDPILNDHDVLVQIHAAGINLLDSKLKSGEFKLILPYQLPLILGHDFAGIILKVGPKVQK
FKIGEEVFGRVRDFRIGTFAEQIAVSEDDIAKKPKHLTMEEAASIPLVGLTSWQALVENANIQKGQKVFIQAGSGGVGTF
AIQLAKVLGAEVATTTSHSNFELVKRLGADTIIDYKTTDFESILKDYDVVIHSQDGKTLHKSLRILKPGGKLISISGPPD
INFAKEMKFPWFLQFVIRMLSLSANKKAKERNVQYSFLFMKANGSQLNQISNLINEGRIQPVIDKIYPFEALNDALAYVE
SGRAKGKVVVKML
>Mature_333_residues
MKAFTINRYNKHQNLQLTEVSDPILNDHDVLVQIHAAGINLLDSKLKSGEFKLILPYQLPLILGHDFAGIILKVGPKVQK
FKIGEEVFGRVRDFRIGTFAEQIAVSEDDIAKKPKHLTMEEAASIPLVGLTSWQALVENANIQKGQKVFIQAGSGGVGTF
AIQLAKVLGAEVATTTSHSNFELVKRLGADTIIDYKTTDFESILKDYDVVIHSQDGKTLHKSLRILKPGGKLISISGPPD
INFAKEMKFPWFLQFVIRMLSLSANKKAKERNVQYSFLFMKANGSQLNQISNLINEGRIQPVIDKIYPFEALNDALAYVE
SGRAKGKVVVKML

Specific function: Unknown

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI47519420, Length=361, Percent_Identity=32.9639889196676, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI22538446, Length=344, Percent_Identity=29.0697674418605, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI22538444, Length=344, Percent_Identity=29.0697674418605, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI18379349, Length=312, Percent_Identity=28.8461538461538, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI194239674, Length=347, Percent_Identity=26.8011527377522, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI13236495, Length=347, Percent_Identity=26.8011527377522, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI194239676, Length=204, Percent_Identity=28.921568627451, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI24308257, Length=347, Percent_Identity=23.9193083573487, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI41872631, Length=322, Percent_Identity=25.776397515528, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI28557745, Length=357, Percent_Identity=25.2100840336134, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI90669511, Length=159, Percent_Identity=32.0754716981132, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI67078404, Length=236, Percent_Identity=25.4237288135593, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1790485, Length=236, Percent_Identity=30.0847457627119, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1789651, Length=201, Percent_Identity=28.3582089552239, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI71987554, Length=354, Percent_Identity=26.8361581920904, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17507255, Length=343, Percent_Identity=26.8221574344023, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17562584, Length=258, Percent_Identity=25.1937984496124, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319520, Length=347, Percent_Identity=24.4956772334294, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24581341, Length=362, Percent_Identity=28.1767955801105, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24581339, Length=363, Percent_Identity=28.3746556473829, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI221330659, Length=316, Percent_Identity=27.5316455696203, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI19920632, Length=316, Percent_Identity=27.5316455696203, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24581345, Length=316, Percent_Identity=26.2658227848101, Blast_Score=81, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 36988; Mature: 36988

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAFTINRYNKHQNLQLTEVSDPILNDHDVLVQIHAAGINLLDSKLKSGEFKLILPYQLP
CCEEEECCCCCCCCEEEEECCCCCCCCCEEEEEEEECCCHHHHHHHCCCCEEEEECEECC
LILGHDFAGIILKVGPKVQKFKIGEEVFGRVRDFRIGTFAEQIAVSEDDIAKKPKHLTME
EEECCCHHEEEEECCCCCEEEECCHHHHHHHHHCCHHHHHHHHHCCCHHHHCCCCCCCHH
EAASIPLVGLTSWQALVENANIQKGQKVFIQAGSGGVGTFAIQLAKVLGAEVATTTSHSN
HHCCCCEEEHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCHHEECCCCCH
FELVKRLGADTIIDYKTTDFESILKDYDVVIHSQDGKTLHKSLRILKPGGKLISISGPPD
HHHHHHCCCCEEEEECCCCHHHHHHHCEEEEECCCCHHHHHHHEEECCCCCEEEECCCCC
INFAKEMKFPWFLQFVIRMLSLSANKKAKERNVQYSFLFMKANGSQLNQISNLINEGRIQ
CCHHHHCCCHHHHHHHHHHHHCCCCCCHHHCCCEEEEEEEECCCCHHHHHHHHHHCCCCC
PVIDKIYPFEALNDALAYVESGRAKGKVVVKML
HHHHHHCCHHHHHHHHHHHHCCCCCCEEEEEEC
>Mature Secondary Structure
MKAFTINRYNKHQNLQLTEVSDPILNDHDVLVQIHAAGINLLDSKLKSGEFKLILPYQLP
CCEEEECCCCCCCCEEEEECCCCCCCCCEEEEEEEECCCHHHHHHHCCCCEEEEECEECC
LILGHDFAGIILKVGPKVQKFKIGEEVFGRVRDFRIGTFAEQIAVSEDDIAKKPKHLTME
EEECCCHHEEEEECCCCCEEEECCHHHHHHHHHCCHHHHHHHHHCCCHHHHCCCCCCCHH
EAASIPLVGLTSWQALVENANIQKGQKVFIQAGSGGVGTFAIQLAKVLGAEVATTTSHSN
HHCCCCEEEHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCHHEECCCCCH
FELVKRLGADTIIDYKTTDFESILKDYDVVIHSQDGKTLHKSLRILKPGGKLISISGPPD
HHHHHHCCCCEEEEECCCCHHHHHHHCEEEEECCCCHHHHHHHEEECCCCCEEEECCCCC
INFAKEMKFPWFLQFVIRMLSLSANKKAKERNVQYSFLFMKANGSQLNQISNLINEGRIQ
CCHHHHCCCHHHHHHHHHHHHCCCCCCHHHCCCEEEEEEEECCCCHHHHHHHHHHCCCCC
PVIDKIYPFEALNDALAYVESGRAKGKVVVKML
HHHHHHCCHHHHHHHHHHHHCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.26 {1,4-naphthoquinone}} 0.065 {1,4-benzoquinone}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.0024 {1,2-naphthoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]