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The map label for this gene is glnA [H]

Identifier: 183220807

GI number: 183220807

Start: 1480399

End: 1481859

Strand: Reverse

Name: glnA [H]

Synonym: LEPBI_I1418

Alternate gene names: 183220807

Gene position: 1481859-1480399 (Counterclockwise)

Preceding gene: 183220809

Following gene: 183220806

Centisome position: 41.17

GC content: 40.31

Gene sequence:

>1461_bases
ATGCAGTTCGCAACCCCAAAATTTACTTCCGGAAAGGAAGTGGTTGAGTATGCCAAAAAAAATGGAGTCCTTTTCTTTGA
CTTCCGATTTACGGATATCAAAGGAATGTGGCACCACGTTTCGTATTATGTGAATTCCGTTAATGAAGATACATTCAAAG
GAATTCCTTTTGATGGATCTTCGATTGCTCGTTGGCAGCCAATCAATGCTTCCGACATGCAATTACATCCAGAAATTTCT
ACGGCATTTTTAGATCCGTTCACAGCTGACAAAACAATCGTTATGTTTTGTGACGTTTGGGACATCTATAAAAACCAATA
CTACGAAAAATGCCCAAGATCCATTGCGAAAAAAGCATTAGAATTCATGAACAAATCGGGTATTGCGGACACAGCATACT
TTGGTCCAGAAAATGAATTTTTCGTTTTTGATAGTTTGAAAGTACGTGATGAAATCAACTGCCAATACTACGAATTGGAT
TCAAACGAAGGGATCTGGAACACTCACTCTGAAATTCCAGGCTCCAATAACACAGGTAAAATCAACTTTAACTCGGGACA
CCGTCCTGGAACGAAAGGTGGTTATTTCCCAGTGGCTCCTATTGACTCACAAGTTGACCTTCGCGCTGAATTTGTAAAAA
CTTTGGAAGCGATTGGAATGGAAACGTTTGTGGTCCACCACGAAGTTGCCCAAGCGCAAGGTGAAATCGGAATTAAATTC
GGAACTTTGATTGAAGCTGCTGATAATGTTCAAAAGTTAAAATACATCGTTAAGATGGTTGCTCATAAACACGGAAAAAC
AGCTACTTTTATGCCAAAACCACTTTTTGGTGATAATGGTAACGGTATGCACGTACATATCTCTCTTTGGAAAGGTGGAA
AAAACCTTTTTGCTGGAGATAAATACCAAGGATTGTCTGATTTTGCATTCAACTATGTTGGTGGGGTGTTAAAGTATGCG
AGAGCTTGTGCAGCATTTACAAATGCATCCACTAACTCTTACAAACGACTCATTCCTGGATTTGAAGCACCTTCCATTTT
AGCATATTCTGCACAAAACCGTTCTGCTTCTTGCCGAATTCCTTTTGTCAGTGGCGAAAAAGCAAAACGTGTGGAATTCA
GATTCCCTGATTCAACAGCTAACCCTTATTTGGCGTTCGCTTCTCTTTTAATGGCTGGTATGGCTGGTGTCGCTGAAAAA
ATCGATCCAGGTCCTGCACGTGAAGAAGATTTATTTGAGCTTTCATTGGATGAAATCCGTGAAAAAGGAATCCGACAAAT
GCCTCACACACTCCGTGAAGCAATGGAAGAGATGCTCGCACAAAGAGAAATCTTCAAACAAGGAGATGTTTTCACAGAAA
ACTTTCTCCAAACATACCAACACTATAAGTTTGAAACAGAAATTTGGCCATGGGAAGGTCGCCCTCACCCATACGAATTC
CTCACAACTTACTCTTGCTAA

Upstream 100 bases:

>100_bases
AACCCTTATATTTCGGTCGAAATGCCTTGGTATAAATCTTGCATTGAAAATGGTAAAAGATTACAACAATCAGACCGACC
TCTGAACGAAGGAGAATCAA

Downstream 100 bases:

>100_bases
GAGAAATCGCCCCGGCAACTGTCGGGGCTTTTTTTTGTTTACCATTCCCTAACCATTCAAAAAATGACAAAAGAACTAAA
GATTTAGTTCAACCAAACGA

Product: glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MQFATPKFTSGKEVVEYAKKNGVLFFDFRFTDIKGMWHHVSYYVNSVNEDTFKGIPFDGSSIARWQPINASDMQLHPEIS
TAFLDPFTADKTIVMFCDVWDIYKNQYYEKCPRSIAKKALEFMNKSGIADTAYFGPENEFFVFDSLKVRDEINCQYYELD
SNEGIWNTHSEIPGSNNTGKINFNSGHRPGTKGGYFPVAPIDSQVDLRAEFVKTLEAIGMETFVVHHEVAQAQGEIGIKF
GTLIEAADNVQKLKYIVKMVAHKHGKTATFMPKPLFGDNGNGMHVHISLWKGGKNLFAGDKYQGLSDFAFNYVGGVLKYA
RACAAFTNASTNSYKRLIPGFEAPSILAYSAQNRSASCRIPFVSGEKAKRVEFRFPDSTANPYLAFASLLMAGMAGVAEK
IDPGPAREEDLFELSLDEIREKGIRQMPHTLREAMEEMLAQREIFKQGDVFTENFLQTYQHYKFETEIWPWEGRPHPYEF
LTTYSC

Sequences:

>Translated_486_residues
MQFATPKFTSGKEVVEYAKKNGVLFFDFRFTDIKGMWHHVSYYVNSVNEDTFKGIPFDGSSIARWQPINASDMQLHPEIS
TAFLDPFTADKTIVMFCDVWDIYKNQYYEKCPRSIAKKALEFMNKSGIADTAYFGPENEFFVFDSLKVRDEINCQYYELD
SNEGIWNTHSEIPGSNNTGKINFNSGHRPGTKGGYFPVAPIDSQVDLRAEFVKTLEAIGMETFVVHHEVAQAQGEIGIKF
GTLIEAADNVQKLKYIVKMVAHKHGKTATFMPKPLFGDNGNGMHVHISLWKGGKNLFAGDKYQGLSDFAFNYVGGVLKYA
RACAAFTNASTNSYKRLIPGFEAPSILAYSAQNRSASCRIPFVSGEKAKRVEFRFPDSTANPYLAFASLLMAGMAGVAEK
IDPGPAREEDLFELSLDEIREKGIRQMPHTLREAMEEMLAQREIFKQGDVFTENFLQTYQHYKFETEIWPWEGRPHPYEF
LTTYSC
>Mature_486_residues
MQFATPKFTSGKEVVEYAKKNGVLFFDFRFTDIKGMWHHVSYYVNSVNEDTFKGIPFDGSSIARWQPINASDMQLHPEIS
TAFLDPFTADKTIVMFCDVWDIYKNQYYEKCPRSIAKKALEFMNKSGIADTAYFGPENEFFVFDSLKVRDEINCQYYELD
SNEGIWNTHSEIPGSNNTGKINFNSGHRPGTKGGYFPVAPIDSQVDLRAEFVKTLEAIGMETFVVHHEVAQAQGEIGIKF
GTLIEAADNVQKLKYIVKMVAHKHGKTATFMPKPLFGDNGNGMHVHISLWKGGKNLFAGDKYQGLSDFAFNYVGGVLKYA
RACAAFTNASTNSYKRLIPGFEAPSILAYSAQNRSASCRIPFVSGEKAKRVEFRFPDSTANPYLAFASLLMAGMAGVAEK
IDPGPAREEDLFELSLDEIREKGIRQMPHTLREAMEEMLAQREIFKQGDVFTENFLQTYQHYKFETEIWPWEGRPHPYEF
LTTYSC

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=479, Percent_Identity=49.0605427974948, Blast_Score=458, Evalue=1e-130,
Organism=Escherichia coli, GI87081870, Length=184, Percent_Identity=30.9782608695652, Blast_Score=93, Evalue=5e-20,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 55080; Mature: 55080

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFATPKFTSGKEVVEYAKKNGVLFFDFRFTDIKGMWHHVSYYVNSVNEDTFKGIPFDGS
CCCCCCCCCCCHHHHHHHHHCCEEEEEEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCC
SIARWQPINASDMQLHPEISTAFLDPFTADKTIVMFCDVWDIYKNQYYEKCPRSIAKKAL
CCCEECCCCCCCCEECCCHHHHHCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHH
EFMNKSGIADTAYFGPENEFFVFDSLKVRDEINCQYYELDSNEGIWNTHSEIPGSNNTGK
HHHHCCCCCCCEEECCCCCEEEEECEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCEE
INFNSGHRPGTKGGYFPVAPIDSQVDLRAEFVKTLEAIGMETFVVHHEVAQAQGEIGIKF
EEECCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCHHEEHHHHHHHCCCCCCEEH
GTLIEAADNVQKLKYIVKMVAHKHGKTATFMPKPLFGDNGNGMHVHISLWKGGKNLFAGD
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEEEEEEECCCCCCCCC
KYQGLSDFAFNYVGGVLKYARACAAFTNASTNSYKRLIPGFEAPSILAYSAQNRSASCRI
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCEEEEECCCCCCEEEE
PFVSGEKAKRVEFRFPDSTANPYLAFASLLMAGMAGVAEKIDPGPAREEDLFELSLDEIR
EEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
EKGIRQMPHTLREAMEEMLAQREIFKQGDVFTENFLQTYQHYKFETEIWPWEGRPHPYEF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCEEECEEECCCCCCCCHHH
LTTYSC
EEECCC
>Mature Secondary Structure
MQFATPKFTSGKEVVEYAKKNGVLFFDFRFTDIKGMWHHVSYYVNSVNEDTFKGIPFDGS
CCCCCCCCCCCHHHHHHHHHCCEEEEEEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCC
SIARWQPINASDMQLHPEISTAFLDPFTADKTIVMFCDVWDIYKNQYYEKCPRSIAKKAL
CCCEECCCCCCCCEECCCHHHHHCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHH
EFMNKSGIADTAYFGPENEFFVFDSLKVRDEINCQYYELDSNEGIWNTHSEIPGSNNTGK
HHHHCCCCCCCEEECCCCCEEEEECEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCEE
INFNSGHRPGTKGGYFPVAPIDSQVDLRAEFVKTLEAIGMETFVVHHEVAQAQGEIGIKF
EEECCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCHHEEHHHHHHHCCCCCCEEH
GTLIEAADNVQKLKYIVKMVAHKHGKTATFMPKPLFGDNGNGMHVHISLWKGGKNLFAGD
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEEEEEEECCCCCCCCC
KYQGLSDFAFNYVGGVLKYARACAAFTNASTNSYKRLIPGFEAPSILAYSAQNRSASCRI
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCEEEEECCCCCCEEEE
PFVSGEKAKRVEFRFPDSTANPYLAFASLLMAGMAGVAEKIDPGPAREEDLFELSLDEIR
EEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
EKGIRQMPHTLREAMEEMLAQREIFKQGDVFTENFLQTYQHYKFETEIWPWEGRPHPYEF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCEEECEEECCCCCCCCHHH
LTTYSC
EEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]