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The map label for this gene is rlmN

Identifier: 183220754

GI number: 183220754

Start: 1421897

End: 1422958

Strand: Direct

Name: rlmN

Synonym: LEPBI_I1364

Alternate gene names: 183220754

Gene position: 1421897-1422958 (Clockwise)

Preceding gene: 183220752

Following gene: 183220755

Centisome position: 39.5

GC content: 42.84

Gene sequence:

>1062_bases
ATGAAAGAGGAAATACCTGTTCTCAAAGGGAAAACCAAAAAAGAACTAGAAGAGATATGTGTTTCCTTGGGTCTTGAAAA
ATACCGAGCGGCACAAATTTACACGGGAATTTATAAGAGTCGTTATACGACAATTGATCAGTTTACTACCCTCTCCAAAG
AGGTTCGGGAAAAGTTAAAAGAGCACACCCAATACCCTGAAATTGAAATCGGCCGCGATTTGGTTTCCAAAGAAGATGGC
ACTCGTAAATTTACCTTTTATGTGGGTGAAAACAAAGAGATCGAAGCGGTTTGGATTCCCTCAGGGGATGGGGGCCGAAA
AACCATTTGTATTTCCTCTCAAATTGGATGTACCCTCAATTGTAAATTTTGTGCCACAGGCCTTTTGGAATACAAAGGCA
ATTTACAAACCTGGCAAATCCTCGACCAAGTTTTGCAAGTAGAGCGTCTTGTGGGAGACCGAGCGACAAACATTGTATTT
ATGGGAATGGGTGAGCCCATGCACAATTATTTTTCAGTGATGAAAGCAGCCCACATTTTACGTGACAAGGATGCCTTTGG
ACTCGGTGCCCTTCGGATTACGATTTCCACAGCTGGAGTGACAACAGGGATCAATCGTTTCATTGAAAATAAAGAACCTT
TTAATTTTGCCATCTCTCTGAACCACCCAAACCCGAACGCACGTTCTTCTGTCATGGACGTAAACGACAAACATCCATTA
GAAAAACTCATTGATTCTGCCAAACGATTCACAAAGGAACTCGATCGTGCCATCACTTTTGAATATGTGATGATTCCCGA
TGTGAACATGGGCCGGGACAATGCAGAACGGCTCGCAAAAATTGCAAGGTCTGTGAACAAATGTAAGATCAATGTGATCC
CCCTCAATACAGATTTTACGGGATGGCGTAGACCCACTGATGATGAGGTCAAAGATTTTGTCATGCATCTCAAAGCAAAA
ACAACAGCTCCCATTCTCAATCGCCGAAGTCCTGGTCGGGACATCAATGGCGCTTGTGGAATGTTAGCTCTCAAAGGAAT
TCGTAGTGAAACGACAAAGTAA

Upstream 100 bases:

>100_bases
TCCAGCGGGCTTTTTGGGGTAAGACTTTTAGAAAGAATGTAATTTCTTGTCCCGTAAGGTGGCAAAGAAAAGATTGGCTA
ATGGTGAAAGGACCTACACA

Downstream 100 bases:

>100_bases
GTGGATTTTGATTTTGTGTTTGGCTCTTAATGTTTCCAACTGCCAAGACATCGTGGAACAAAAATGCCAAATGGCATGTG
AAAAATTTGTTTCTTGTACA

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MKEEIPVLKGKTKKELEEICVSLGLEKYRAAQIYTGIYKSRYTTIDQFTTLSKEVREKLKEHTQYPEIEIGRDLVSKEDG
TRKFTFYVGENKEIEAVWIPSGDGGRKTICISSQIGCTLNCKFCATGLLEYKGNLQTWQILDQVLQVERLVGDRATNIVF
MGMGEPMHNYFSVMKAAHILRDKDAFGLGALRITISTAGVTTGINRFIENKEPFNFAISLNHPNPNARSSVMDVNDKHPL
EKLIDSAKRFTKELDRAITFEYVMIPDVNMGRDNAERLAKIARSVNKCKINVIPLNTDFTGWRRPTDDEVKDFVMHLKAK
TTAPILNRRSPGRDINGACGMLALKGIRSETTK

Sequences:

>Translated_353_residues
MKEEIPVLKGKTKKELEEICVSLGLEKYRAAQIYTGIYKSRYTTIDQFTTLSKEVREKLKEHTQYPEIEIGRDLVSKEDG
TRKFTFYVGENKEIEAVWIPSGDGGRKTICISSQIGCTLNCKFCATGLLEYKGNLQTWQILDQVLQVERLVGDRATNIVF
MGMGEPMHNYFSVMKAAHILRDKDAFGLGALRITISTAGVTTGINRFIENKEPFNFAISLNHPNPNARSSVMDVNDKHPL
EKLIDSAKRFTKELDRAITFEYVMIPDVNMGRDNAERLAKIARSVNKCKINVIPLNTDFTGWRRPTDDEVKDFVMHLKAK
TTAPILNRRSPGRDINGACGMLALKGIRSETTK
>Mature_353_residues
MKEEIPVLKGKTKKELEEICVSLGLEKYRAAQIYTGIYKSRYTTIDQFTTLSKEVREKLKEHTQYPEIEIGRDLVSKEDG
TRKFTFYVGENKEIEAVWIPSGDGGRKTICISSQIGCTLNCKFCATGLLEYKGNLQTWQILDQVLQVERLVGDRATNIVF
MGMGEPMHNYFSVMKAAHILRDKDAFGLGALRITISTAGVTTGINRFIENKEPFNFAISLNHPNPNARSSVMDVNDKHPL
EKLIDSAKRFTKELDRAITFEYVMIPDVNMGRDNAERLAKIARSVNKCKINVIPLNTDFTGWRRPTDDEVKDFVMHLKAK
TTAPILNRRSPGRDINGACGMLALKGIRSETTK

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=343, Percent_Identity=33.8192419825073, Blast_Score=184, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_LEPBA (B0SGA8)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001962410.1
- GeneID:   6388235
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_1312
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- ProtClustDB:   PRK14465
- BioCyc:   LBIF355278:LBF_1312-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 39890; Mature: 39890

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEEIPVLKGKTKKELEEICVSLGLEKYRAAQIYTGIYKSRYTTIDQFTTLSKEVREKLK
CCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
EHTQYPEIEIGRDLVSKEDGTRKFTFYVGENKEIEAVWIPSGDGGRKTICISSQIGCTLN
HHCCCCCHHHCHHHHCCCCCCEEEEEEECCCCCEEEEEEECCCCCCEEEEEECCCCCEEE
CKFCATGLLEYKGNLQTWQILDQVLQVERLVGDRATNIVFMGMGEPMHNYFSVMKAAHIL
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHH
RDKDAFGLGALRITISTAGVTTGINRFIENKEPFNFAISLNHPNPNARSSVMDVNDKHPL
HCCCCCCEEEEEEEEEECCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCEECCCCCCHH
EKLIDSAKRFTKELDRAITFEYVMIPDVNMGRDNAERLAKIARSVNKCKINVIPLNTDFT
HHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCC
GWRRPTDDEVKDFVMHLKAKTTAPILNRRSPGRDINGACGMLALKGIRSETTK
CCCCCCHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKEEIPVLKGKTKKELEEICVSLGLEKYRAAQIYTGIYKSRYTTIDQFTTLSKEVREKLK
CCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
EHTQYPEIEIGRDLVSKEDGTRKFTFYVGENKEIEAVWIPSGDGGRKTICISSQIGCTLN
HHCCCCCHHHCHHHHCCCCCCEEEEEEECCCCCEEEEEEECCCCCCEEEEEECCCCCEEE
CKFCATGLLEYKGNLQTWQILDQVLQVERLVGDRATNIVFMGMGEPMHNYFSVMKAAHIL
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHH
RDKDAFGLGALRITISTAGVTTGINRFIENKEPFNFAISLNHPNPNARSSVMDVNDKHPL
HCCCCCCEEEEEEEEEECCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCEECCCCCCHH
EKLIDSAKRFTKELDRAITFEYVMIPDVNMGRDNAERLAKIARSVNKCKINVIPLNTDFT
HHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCC
GWRRPTDDEVKDFVMHLKAKTTAPILNRRSPGRDINGACGMLALKGIRSETTK
CCCCCCHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA