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The map label for this gene is gdhB [H]

Identifier: 183220751

GI number: 183220751

Start: 1417018

End: 1418316

Strand: Direct

Name: gdhB [H]

Synonym: LEPBI_I1361

Alternate gene names: 183220751

Gene position: 1417018-1418316 (Clockwise)

Preceding gene: 183220749

Following gene: 183220752

Centisome position: 39.37

GC content: 41.65

Gene sequence:

>1299_bases
TTGCGGATACAAATCCAAGCGAATTATCCTATGACAAGTAACAGTTGTATGAAAAACCAAATCATAATCATTCTATCCCT
TCTTTCCGTTTCCCTTTCCTCCCTTGCCTGTGATGATTTGGGTCGAAAGATCTTAAAAACATTCAGCAAAAAATATGAAA
CCGATGGAAAGGTTTTGGGTGGCAAACCGATTTTTATTGGACCCGATGCCCAAAGAAAACAACTTTCAATTTCACTACAG
GAAGTCGTCAAAGTAAAGGAACCAACCGACATCCAATTCCCACCAGGGGACAGTCCCTTTTTATTCGTTTTGGAAAAAGC
AGGGGATCTCATTTTATTTGATCGCGATAAAAAAAACAAACGAGTGTTAAAGTCTTTTCAAGTGATCACTGATAGCGAAG
AAGGTCTTCTTGGTTTTACCTTCCATCCAAAGTATCCCAAAGAACCAAAGGTATACACTCATACTGTGATCAGTTCAGCA
AACCGTGATATGACGGTGATTGCAGAATGGGAAGTGGAAAATCCAAATTCCTTTGAAACGATGGCTTTAAAAAACGAACG
AGTCTTACTTGAGGTGGAACAACCATACCCGAATCACAATGGGGGGCAAATCACATTTGGTCCCGATGGACATCTGTACA
TTGGTCTTGGGGATGGTGGCTGGCGGGCTGATCCAAAAAATAACGGACAAAATCCGAACACCTTACTTGGCTCCATCCTA
CGAATCTCGCCCGTTCCCGATCCGATTGGAAAAAAACCTTATTCCATTCCGAAAGACAATCCTTTTATCGGAAAACAAGG
TTTCCTTCCTGAAATCTTTGCTTACGGGATTCGGAATCCATGGAAGATGAGTTTTTCGCCTGACGGACGTTTGATTGCCG
CAGATGTGGGACAAGATGCCTATGAAGAAGTGGATATCATCCTTTCAGGGAAAAATTACGGATGGAACCAAACAGAAGGG
TTCCATTGTTTTACGGATGGATGTAATCCTTCTCTTTACCAATCTCCGTTCTATGAATACGGAAGGGAAGAAGGGCAATC
CATCACAGGTGGGTATGTGTATTTTGGAACTTCCATCCCTGAGTTAAAAGGCAAATATGTGTTTGGTGATTTTATCCAAG
GGAAAATTTGGGCTGTTGGCATTCCAAAACCTGGTGAAAATACCAAAATGACGGAAACAATCGCTCTTGGAAAATGGAAT
ATCCTCATCCCAACATTTGGCCAAGACAGCGAAGGAGAAATCTTTGTTGCCGACTACCAAACAGGAACCATATACAAAAT
TGTGAAACAGATTCCGTAA

Upstream 100 bases:

>100_bases
ATAGGGATCGGGATCGTTCAGTTGTAAATAGGCGAATAAAAAGAAAATGGGAACACAGAGAAGAGAAAAAAGTTTCATCA
CAGTTTCCAAAGTTTTTTGA

Downstream 100 bases:

>100_bases
TCCCAAGATGACCCTTGAAACAAGTATCGCTTAATTCAATTTAGTAGAGACCAAAGAAACCGTTTGATATGAAAAAATTC
TTTTCCCATATTCCATTTTA

Product: putative glucose dehydrogenase

Products: NA

Alternate protein names: Glucose dehydrogenase B [pyrroloquinoline-quinone]; Soluble glucose dehydrogenase; s-GDH [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MRIQIQANYPMTSNSCMKNQIIIILSLLSVSLSSLACDDLGRKILKTFSKKYETDGKVLGGKPIFIGPDAQRKQLSISLQ
EVVKVKEPTDIQFPPGDSPFLFVLEKAGDLILFDRDKKNKRVLKSFQVITDSEEGLLGFTFHPKYPKEPKVYTHTVISSA
NRDMTVIAEWEVENPNSFETMALKNERVLLEVEQPYPNHNGGQITFGPDGHLYIGLGDGGWRADPKNNGQNPNTLLGSIL
RISPVPDPIGKKPYSIPKDNPFIGKQGFLPEIFAYGIRNPWKMSFSPDGRLIAADVGQDAYEEVDIILSGKNYGWNQTEG
FHCFTDGCNPSLYQSPFYEYGREEGQSITGGYVYFGTSIPELKGKYVFGDFIQGKIWAVGIPKPGENTKMTETIALGKWN
ILIPTFGQDSEGEIFVADYQTGTIYKIVKQIP

Sequences:

>Translated_432_residues
MRIQIQANYPMTSNSCMKNQIIIILSLLSVSLSSLACDDLGRKILKTFSKKYETDGKVLGGKPIFIGPDAQRKQLSISLQ
EVVKVKEPTDIQFPPGDSPFLFVLEKAGDLILFDRDKKNKRVLKSFQVITDSEEGLLGFTFHPKYPKEPKVYTHTVISSA
NRDMTVIAEWEVENPNSFETMALKNERVLLEVEQPYPNHNGGQITFGPDGHLYIGLGDGGWRADPKNNGQNPNTLLGSIL
RISPVPDPIGKKPYSIPKDNPFIGKQGFLPEIFAYGIRNPWKMSFSPDGRLIAADVGQDAYEEVDIILSGKNYGWNQTEG
FHCFTDGCNPSLYQSPFYEYGREEGQSITGGYVYFGTSIPELKGKYVFGDFIQGKIWAVGIPKPGENTKMTETIALGKWN
ILIPTFGQDSEGEIFVADYQTGTIYKIVKQIP
>Mature_432_residues
MRIQIQANYPMTSNSCMKNQIIIILSLLSVSLSSLACDDLGRKILKTFSKKYETDGKVLGGKPIFIGPDAQRKQLSISLQ
EVVKVKEPTDIQFPPGDSPFLFVLEKAGDLILFDRDKKNKRVLKSFQVITDSEEGLLGFTFHPKYPKEPKVYTHTVISSA
NRDMTVIAEWEVENPNSFETMALKNERVLLEVEQPYPNHNGGQITFGPDGHLYIGLGDGGWRADPKNNGQNPNTLLGSIL
RISPVPDPIGKKPYSIPKDNPFIGKQGFLPEIFAYGIRNPWKMSFSPDGRLIAADVGQDAYEEVDIILSGKNYGWNQTEG
FHCFTDGCNPSLYQSPFYEYGREEGQSITGGYVYFGTSIPELKGKYVFGDFIQGKIWAVGIPKPGENTKMTETIALGKWN
ILIPTFGQDSEGEIFVADYQTGTIYKIVKQIP

Specific function: Oxidizes glucose to gluconolactone [H]

COG id: COG2133

COG function: function code G; Glucose/sorbosone dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PQQ oxidoreductase gdhB family [H]

Homologues:

Organism=Homo sapiens, GI187936955, Length=395, Percent_Identity=34.6835443037975, Blast_Score=215, Evalue=8e-56,
Organism=Homo sapiens, GI187936953, Length=395, Percent_Identity=34.6835443037975, Blast_Score=215, Evalue=8e-56,
Organism=Homo sapiens, GI124248546, Length=399, Percent_Identity=35.8395989974937, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI20143973, Length=395, Percent_Identity=28.1012658227848, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1787060, Length=235, Percent_Identity=26.3829787234043, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011042
- InterPro:   IPR012938
- InterPro:   IPR019893
- InterPro:   IPR011041 [H]

Pfam domain/function: PF07995 GSDH [H]

EC number: =1.1.5.2 [H]

Molecular weight: Translated: 48203; Mature: 48203

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIQIQANYPMTSNSCMKNQIIIILSLLSVSLSSLACDDLGRKILKTFSKKYETDGKVLG
CEEEEEECCCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEC
GKPIFIGPDAQRKQLSISLQEVVKVKEPTDIQFPPGDSPFLFVLEKAGDLILFDRDKKNK
CCCEEECCCCCCCEEEEEHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCEEEEECCCCHH
RVLKSFQVITDSEEGLLGFTFHPKYPKEPKVYTHTVISSANRDMTVIAEWEVENPNSFET
HHHHHHHEEECCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCEEEEEEEECCCCCCEEE
MALKNERVLLEVEQPYPNHNGGQITFGPDGHLYIGLGDGGWRADPKNNGQNPNTLLGSIL
EEECCCEEEEEECCCCCCCCCCEEEECCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHE
RISPVPDPIGKKPYSIPKDNPFIGKQGFLPEIFAYGIRNPWKMSFSPDGRLIAADVGQDA
EECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEECCCCCEEEEECCCCC
YEEVDIILSGKNYGWNQTEGFHCFTDGCNPSLYQSPFYEYGREEGQSITGGYVYFGTSIP
CCCEEEEEECCCCCCCCCCCEEEEECCCCCCHHCCHHHHHHHHCCCEEECCEEEECCCCC
ELKGKYVFGDFIQGKIWAVGIPKPGENTKMTETIALGKWNILIPTFGQDSEGEIFVADYQ
CCCCCEEEEEECCCEEEEEECCCCCCCCCEEEEEEECEEEEEEEECCCCCCCCEEEEECC
TGTIYKIVKQIP
CCHHHHHHHHCC
>Mature Secondary Structure
MRIQIQANYPMTSNSCMKNQIIIILSLLSVSLSSLACDDLGRKILKTFSKKYETDGKVLG
CEEEEEECCCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEC
GKPIFIGPDAQRKQLSISLQEVVKVKEPTDIQFPPGDSPFLFVLEKAGDLILFDRDKKNK
CCCEEECCCCCCCEEEEEHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCEEEEECCCCHH
RVLKSFQVITDSEEGLLGFTFHPKYPKEPKVYTHTVISSANRDMTVIAEWEVENPNSFET
HHHHHHHEEECCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCEEEEEEEECCCCCCEEE
MALKNERVLLEVEQPYPNHNGGQITFGPDGHLYIGLGDGGWRADPKNNGQNPNTLLGSIL
EEECCCEEEEEECCCCCCCCCCEEEECCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHE
RISPVPDPIGKKPYSIPKDNPFIGKQGFLPEIFAYGIRNPWKMSFSPDGRLIAADVGQDA
EECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEECCCCCEEEEECCCCC
YEEVDIILSGKNYGWNQTEGFHCFTDGCNPSLYQSPFYEYGREEGQSITGGYVYFGTSIP
CCCEEEEEECCCCCCCCCCCEEEEECCCCCCHHCCHHHHHHHHCCCEEECCEEEECCCCC
ELKGKYVFGDFIQGKIWAVGIPKPGENTKMTETIALGKWNILIPTFGQDSEGEIFVADYQ
CCCCCEEEEEECCCEEEEEECCCCCCCCCEEEEEEECEEEEEEEECCCCCCCCEEEEECC
TGTIYKIVKQIP
CCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2671663; 10366508; 10508152 [H]