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The map label for this gene is dhaT [H]

Identifier: 183220716

GI number: 183220716

Start: 1381304

End: 1382470

Strand: Reverse

Name: dhaT [H]

Synonym: LEPBI_I1326

Alternate gene names: 183220716

Gene position: 1382470-1381304 (Counterclockwise)

Preceding gene: 183220717

Following gene: 183220715

Centisome position: 38.41

GC content: 40.96

Gene sequence:

>1167_bases
ATGCCAGTTCTCCCCGAATGGATTAATTTTCAATTTCCTCCCAAGATACATTTCGAAATCGATTGTGGATATAAACTTGG
ATCCTTTGTCAAAAACATTGGATCTCGAGTTGTACTCATCACAACCCAAAAAGAATTAGAAAATTCAGAAGAACTCTCCA
TCATCAAAACGAGTTTAGAAAAACACGCGGAAGGTGTCATCATTTACGATGACATTGTAGACCGTGTGCATTTCAAAGAT
TTGGATACTTGTGCTCATTTTTTACGAATTTCGAATGCTGACTGTGTTGTGGCATATGGTTCTTTTGAATCAATGAACGC
AGGTAAGGCGGCTTCCCTTCTCGCCACCAATGATATGTTTGCGGAAGAACTCCTCGTTGGAAGGAAACAACCGAAAAAAA
AGGGATTACCGCTTGTGGTTGTTCCGACAAAACCTCTACTCGGCAATGAATGTTCTCCATTTTTTTCCATCGTCGATGAC
AAAGACAAAAATAGAAAGTATTTCGCTCATGAATGGGCATTTCCGGAACTCATCGTATCCGATCCAAAAATTGGAGCTGG
TATGTCGAGCTCTGAAACAGCAAAAACAGGTATTTCTATATTATCAGCAGCAGTGGATAGTATTCTTTCCAAATATGCCA
ACGAGATCACTTCTTCTACTGCATTACGTTCCATTGAACTTATTTCTAAAAACATTGTTCCTGCCATCCGAGAACCACGT
AACTTAGGACCAAAAAACTCCATTTATGCGGCAAGTTTACTGGCAGGCATTGCCCAATCCACAAGTAGCCTAGGGCTTTG
TTATGCATTGTCCCTTGCTGTAACAACGGTTACCAACTTAGATATTTTCCAAAGTATGTCGATCCTTCTCCCTCACGTGA
TGGAATACAATCTCACTTCCTCTGCCGGTAAGTATGTAATGATAGCAAGAGCCCTTGATGAGGACGTAACCAATATCTCC
GTCATTGAAGCGGCGATCAAAGCCGTGGAAGGGATTCGTAAAATTTACTTAGAACTACGTATCCCACAACGCCTCTCCGA
GTATGAAGTGAAAAAAATCGACCTACCTGGAATTGCAACTCTTGCAGCTACGTATTCATTTCTTGATTGTCTTCCAAGAG
AACTACCGAAAAATGAAATCGAAACCATCTTAGTGGCTGCGTTTTAG

Upstream 100 bases:

>100_bases
TCCTCGGACAACAGTAATAAGGACAGGTGGTGAGGGATCCTTTGTTATTGTCCTCTCCGAGGATTTCTCACGCTTCGTAC
ATTCATAGTTAGGGGATCGT

Downstream 100 bases:

>100_bases
GATAAACTTGATCCAACTCACCGAATTTGAACAAAAACTCTTAGAAACATTTTCCTTAAGTGACAGGGATGCCAGACGAT
TGCAACGAGTGATCCAAGAT

Product: putative alcohol dehydrogenase

Products: NA

Alternate protein names: 1,3-propanediol oxidoreductase; 3-hydroxypropionaldehyde reductase [H]

Number of amino acids: Translated: 388; Mature: 387

Protein sequence:

>388_residues
MPVLPEWINFQFPPKIHFEIDCGYKLGSFVKNIGSRVVLITTQKELENSEELSIIKTSLEKHAEGVIIYDDIVDRVHFKD
LDTCAHFLRISNADCVVAYGSFESMNAGKAASLLATNDMFAEELLVGRKQPKKKGLPLVVVPTKPLLGNECSPFFSIVDD
KDKNRKYFAHEWAFPELIVSDPKIGAGMSSSETAKTGISILSAAVDSILSKYANEITSSTALRSIELISKNIVPAIREPR
NLGPKNSIYAASLLAGIAQSTSSLGLCYALSLAVTTVTNLDIFQSMSILLPHVMEYNLTSSAGKYVMIARALDEDVTNIS
VIEAAIKAVEGIRKIYLELRIPQRLSEYEVKKIDLPGIATLAATYSFLDCLPRELPKNEIETILVAAF

Sequences:

>Translated_388_residues
MPVLPEWINFQFPPKIHFEIDCGYKLGSFVKNIGSRVVLITTQKELENSEELSIIKTSLEKHAEGVIIYDDIVDRVHFKD
LDTCAHFLRISNADCVVAYGSFESMNAGKAASLLATNDMFAEELLVGRKQPKKKGLPLVVVPTKPLLGNECSPFFSIVDD
KDKNRKYFAHEWAFPELIVSDPKIGAGMSSSETAKTGISILSAAVDSILSKYANEITSSTALRSIELISKNIVPAIREPR
NLGPKNSIYAASLLAGIAQSTSSLGLCYALSLAVTTVTNLDIFQSMSILLPHVMEYNLTSSAGKYVMIARALDEDVTNIS
VIEAAIKAVEGIRKIYLELRIPQRLSEYEVKKIDLPGIATLAATYSFLDCLPRELPKNEIETILVAAF
>Mature_387_residues
PVLPEWINFQFPPKIHFEIDCGYKLGSFVKNIGSRVVLITTQKELENSEELSIIKTSLEKHAEGVIIYDDIVDRVHFKDL
DTCAHFLRISNADCVVAYGSFESMNAGKAASLLATNDMFAEELLVGRKQPKKKGLPLVVVPTKPLLGNECSPFFSIVDDK
DKNRKYFAHEWAFPELIVSDPKIGAGMSSSETAKTGISILSAAVDSILSKYANEITSSTALRSIELISKNIVPAIREPRN
LGPKNSIYAASLLAGIAQSTSSLGLCYALSLAVTTVTNLDIFQSMSILLPHVMEYNLTSSAGKYVMIARALDEDVTNISV
IEAAIKAVEGIRKIYLELRIPQRLSEYEVKKIDLPGIATLAATYSFLDCLPRELPKNEIETILVAAF

Specific function: Most active with substrates containing two primary alcohol groups separated by one or two carbon atoms. In the physiological direction, 3-hydroxypropionaldehyde is the preferred substrate [H]

COG id: COG1454

COG function: function code C; Alcohol dehydrogenase, class IV

Gene ontology:

Cell location: Integral Membrane Protein [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=376, Percent_Identity=22.8723404255319, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI87082107, Length=270, Percent_Identity=29.6296296296296, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1789163, Length=349, Percent_Identity=25.5014326647564, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI48994951, Length=337, Percent_Identity=26.1127596439169, Blast_Score=106, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17537053, Length=383, Percent_Identity=21.6710182767624, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6321181, Length=379, Percent_Identity=25.8575197889182, Blast_Score=105, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24657991, Length=391, Percent_Identity=23.2736572890026, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: =1.1.1.202 [H]

Molecular weight: Translated: 42622; Mature: 42491

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVLPEWINFQFPPKIHFEIDCGYKLGSFVKNIGSRVVLITTQKELENSEELSIIKTSLE
CCCCCCHHCCCCCCCEEEEEECCCHHHHHHHHHCCEEEEEEEHHHHCCCHHHHHHHHHHH
KHAEGVIIYDDIVDRVHFKDLDTCAHFLRISNADCVVAYGSFESMNAGKAASLLATNDMF
HHCCCEEEEHHHHHHHHHCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHCCCHHH
AEELLVGRKQPKKKGLPLVVVPTKPLLGNECSPFFSIVDDKDKNRKYFAHEWAFPELIVS
HHHHHHCCCCCHHCCCCEEEECCCCCCCCCCCCHHHHCCCCCCCCCEEEECCCCCHHEEC
DPKIGAGMSSSETAKTGISILSAAVDSILSKYANEITSSTALRSIELISKNIVPAIREPR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCC
NLGPKNSIYAASLLAGIAQSTSSLGLCYALSLAVTTVTNLDIFQSMSILLPHVMEYNLTS
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SAGKYVMIARALDEDVTNISVIEAAIKAVEGIRKIYLELRIPQRLSEYEVKKIDLPGIAT
CCCCEEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHEECCHHHCCCCCEEECCCCHHH
LAATYSFLDCLPRELPKNEIETILVAAF
HHHHHHHHHHHHHHCCHHHHCEEEEEEC
>Mature Secondary Structure 
PVLPEWINFQFPPKIHFEIDCGYKLGSFVKNIGSRVVLITTQKELENSEELSIIKTSLE
CCCCCHHCCCCCCCEEEEEECCCHHHHHHHHHCCEEEEEEEHHHHCCCHHHHHHHHHHH
KHAEGVIIYDDIVDRVHFKDLDTCAHFLRISNADCVVAYGSFESMNAGKAASLLATNDMF
HHCCCEEEEHHHHHHHHHCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHCCCHHH
AEELLVGRKQPKKKGLPLVVVPTKPLLGNECSPFFSIVDDKDKNRKYFAHEWAFPELIVS
HHHHHHCCCCCHHCCCCEEEECCCCCCCCCCCCHHHHCCCCCCCCCEEEECCCCCHHEEC
DPKIGAGMSSSETAKTGISILSAAVDSILSKYANEITSSTALRSIELISKNIVPAIREPR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCC
NLGPKNSIYAASLLAGIAQSTSSLGLCYALSLAVTTVTNLDIFQSMSILLPHVMEYNLTS
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SAGKYVMIARALDEDVTNISVIEAAIKAVEGIRKIYLELRIPQRLSEYEVKKIDLPGIAT
CCCCEEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHEECCHHHCCCCCEEECCCCHHH
LAATYSFLDCLPRELPKNEIETILVAAF
HHHHHHHHHHHHHHCCHHHHCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7721705 [H]