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The map label for this gene is pilJ [H]

Identifier: 183220626

GI number: 183220626

Start: 1282807

End: 1284360

Strand: Reverse

Name: pilJ [H]

Synonym: LEPBI_I1232

Alternate gene names: 183220626

Gene position: 1284360-1282807 (Counterclockwise)

Preceding gene: 183220627

Following gene: 183220625

Centisome position: 35.68

GC content: 35.07

Gene sequence:

>1554_bases
TTGTTTGCTAAATTTTTTTCCCCGAAGAAAGCCATGACAATTTCCGATGACCTTTTCACGGAAGTAATGATACGAAACAA
CAAACGAATGTTCTTGTCATTTCTTTCGATCTTAGCTCTTGCCAACGTGGCAACACTTGCCATTAAAATTGCCGGCAAAG
GCTCAGATTACTTGACCTACGAAAGTATCTTCATCGAGTTTATTTTAGCTACGTCTATATTGATTGTTGGTTTTTTATTA
TCTTCTAGATTAAAAACACATTGGGTTTCAAGTTATATCTCCATTACAGGTGTTACATTATGTTTATTGGTATTCCAATA
CGTAATTTATGGGGCAACCGAACTATCTGCAACATTTTATATCTCTTTTGTTTTAAGCGTATTGTATTTCAATCGTAACG
CATCCATTTATAACTTTATCTTAATCATTGTATCCGAAATTGTTTTATTTGTTTTAAGACCTGAATTAATTCCAGGTGGT
CCAAAAAGTAATATCATCGTAAGGTTTTTGATTTTTGTTTGGGTCGGTATTGGTGCGACTGTCGGAGCAACTGCAACAAG
AATTCTTCTAAACTTGGCTGTAGAAAAACAAAAAGAAGCAAAGAAAGTTTTGGACAATCTATTAGAAATGGCCAAAACCA
TTCTTAACACGATTGATTTGATGAAACAACAAATTAAGAACCAAGATACAATTTCAGAAGAACTGAAACAAATTTCAGAA
CACCAAGCATCATCCTTATTTGAAATTTCAACATCTTTACAAGAATTATCTTCTAAAGCTGATTCCAATAATAAAATTGC
AAAATCTTTATACCAAGAGTCGGAAACTTCAATTCAATCTGTGAATGACTTAAAAGCCATCAACGAAACCGTACAAACCG
GAACAGGTAGTATCTATAAAAATTTAGATGTGGTTACAGAATATTCAAACGACACTTCAAAACACATTCACCTTTCCATT
GATAAGTTCAATATCCTTCAGGAAAAAAGTAGGGAAATTTCTGAATTTGTCTCTGTCATCAATGACATTGCTGACAAAGT
AAACTTGTTATCTTTGAATGCTGCGATCGAGGCGGCAAGAGCCGGAGAACATGGACGAGGTTTTGCGGTTGTTGCAGAAG
AAATCTCCAAGTTGGCTCTTGCCACGACAAAAAACTCCAAGGAAATTTCTAAAATCATCAAAGAAAACCTTTCATTGATT
GGTGAAAGTAGTGAACTCATCAACCGGTCCTCGGAGATGATGGGAAAACTAGACACATCCATTGGTATCATTAAAAATGA
AATCACGGGAGTTGGTTCTAAAATCGTAGAAATTGATAAAGCAATCCAAACCATTGACAACTTAAATACACGAATTTATG
AAACGAGTAGGACCATTGAAACCTCCACAAACTTCCAAAAAGTAGCAACAGAAGAGTCAACAAAAATTACTGCAAACATC
TCTGAGTATGCAACGAACATTGTGCAAATTTCCAAACAAATTTCGGAAAGTAGCAAATCTACAGGGGGGATCATTTCTAA
ACTCGATTCGATGGCGAAAGAGATGACGAATTGA

Upstream 100 bases:

>100_bases
TAGTTTCCAAACAACTGTCGGGTTTTAGGACGTTTCTTGCCAAAAGATTCCGTCTGATAAAAATGACTTAAACCCATACC
CATTTTCAAGAAGGTACAGT

Downstream 100 bases:

>100_bases
GGATTCTTTTGTTAGAAGTTTGCCGATGTATCCAATCAAAAAATGAAAAGATTGAAACTCAATTGGTGGTCTTACCAAAC
CACAATGGCAGAAGGAAAAT

Product: putative methyl-accepting chemotaxis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 517; Mature: 517

Protein sequence:

>517_residues
MFAKFFSPKKAMTISDDLFTEVMIRNNKRMFLSFLSILALANVATLAIKIAGKGSDYLTYESIFIEFILATSILIVGFLL
SSRLKTHWVSSYISITGVTLCLLVFQYVIYGATELSATFYISFVLSVLYFNRNASIYNFILIIVSEIVLFVLRPELIPGG
PKSNIIVRFLIFVWVGIGATVGATATRILLNLAVEKQKEAKKVLDNLLEMAKTILNTIDLMKQQIKNQDTISEELKQISE
HQASSLFEISTSLQELSSKADSNNKIAKSLYQESETSIQSVNDLKAINETVQTGTGSIYKNLDVVTEYSNDTSKHIHLSI
DKFNILQEKSREISEFVSVINDIADKVNLLSLNAAIEAARAGEHGRGFAVVAEEISKLALATTKNSKEISKIIKENLSLI
GESSELINRSSEMMGKLDTSIGIIKNEITGVGSKIVEIDKAIQTIDNLNTRIYETSRTIETSTNFQKVATEESTKITANI
SEYATNIVQISKQISESSKSTGGIISKLDSMAKEMTN

Sequences:

>Translated_517_residues
MFAKFFSPKKAMTISDDLFTEVMIRNNKRMFLSFLSILALANVATLAIKIAGKGSDYLTYESIFIEFILATSILIVGFLL
SSRLKTHWVSSYISITGVTLCLLVFQYVIYGATELSATFYISFVLSVLYFNRNASIYNFILIIVSEIVLFVLRPELIPGG
PKSNIIVRFLIFVWVGIGATVGATATRILLNLAVEKQKEAKKVLDNLLEMAKTILNTIDLMKQQIKNQDTISEELKQISE
HQASSLFEISTSLQELSSKADSNNKIAKSLYQESETSIQSVNDLKAINETVQTGTGSIYKNLDVVTEYSNDTSKHIHLSI
DKFNILQEKSREISEFVSVINDIADKVNLLSLNAAIEAARAGEHGRGFAVVAEEISKLALATTKNSKEISKIIKENLSLI
GESSELINRSSEMMGKLDTSIGIIKNEITGVGSKIVEIDKAIQTIDNLNTRIYETSRTIETSTNFQKVATEESTKITANI
SEYATNIVQISKQISESSKSTGGIISKLDSMAKEMTN
>Mature_517_residues
MFAKFFSPKKAMTISDDLFTEVMIRNNKRMFLSFLSILALANVATLAIKIAGKGSDYLTYESIFIEFILATSILIVGFLL
SSRLKTHWVSSYISITGVTLCLLVFQYVIYGATELSATFYISFVLSVLYFNRNASIYNFILIIVSEIVLFVLRPELIPGG
PKSNIIVRFLIFVWVGIGATVGATATRILLNLAVEKQKEAKKVLDNLLEMAKTILNTIDLMKQQIKNQDTISEELKQISE
HQASSLFEISTSLQELSSKADSNNKIAKSLYQESETSIQSVNDLKAINETVQTGTGSIYKNLDVVTEYSNDTSKHIHLSI
DKFNILQEKSREISEFVSVINDIADKVNLLSLNAAIEAARAGEHGRGFAVVAEEISKLALATTKNSKEISKIIKENLSLI
GESSELINRSSEMMGKLDTSIGIIKNEITGVGSKIVEIDKAIQTIDNLNTRIYETSRTIETSTNFQKVATEESTKITANI
SEYATNIVQISKQISESSKSTGGIISKLDSMAKEMTN

Specific function: May be a part of a signal-transduction system that regulates twitching motility by controlling pilus function (extension and retraction) [H]

COG id: COG0840

COG function: function code NT; Methyl-accepting chemotaxis protein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI2367378, Length=321, Percent_Identity=26.1682242990654, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1788195, Length=231, Percent_Identity=31.1688311688312, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1788194, Length=240, Percent_Identity=28.3333333333333, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1787690, Length=128, Percent_Identity=39.0625, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789453, Length=211, Percent_Identity=26.5402843601896, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004090
- InterPro:   IPR004089
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00015 MCPsignal [H]

EC number: NA

Molecular weight: Translated: 57158; Mature: 57158

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS50111 CHEMOTAXIS_TRANSDUC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFAKFFSPKKAMTISDDLFTEVMIRNNKRMFLSFLSILALANVATLAIKIAGKGSDYLTY
CCCCCCCCCCCCEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHH
ESIFIEFILATSILIVGFLLSSRLKTHWVSSYISITGVTLCLLVFQYVIYGATELSATFY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
ISFVLSVLYFNRNASIYNFILIIVSEIVLFVLRPELIPGGPKSNIIVRFLIFVWVGIGAT
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCH
VGATATRILLNLAVEKQKEAKKVLDNLLEMAKTILNTIDLMKQQIKNQDTISEELKQISE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
HQASSLFEISTSLQELSSKADSNNKIAKSLYQESETSIQSVNDLKAINETVQTGTGSIYK
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
NLDVVTEYSNDTSKHIHLSIDKFNILQEKSREISEFVSVINDIADKVNLLSLNAAIEAAR
CHHHHEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGEHGRGFAVVAEEISKLALATTKNSKEISKIIKENLSLIGESSELINRSSEMMGKLDTS
CCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
IGIIKNEITGVGSKIVEIDKAIQTIDNLNTRIYETSRTIETSTNFQKVATEESTKITANI
HHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHEEECCCCEECCCCHHHHHCCCCCEEEEEH
SEYATNIVQISKQISESSKSTGGIISKLDSMAKEMTN
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFAKFFSPKKAMTISDDLFTEVMIRNNKRMFLSFLSILALANVATLAIKIAGKGSDYLTY
CCCCCCCCCCCCEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHH
ESIFIEFILATSILIVGFLLSSRLKTHWVSSYISITGVTLCLLVFQYVIYGATELSATFY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
ISFVLSVLYFNRNASIYNFILIIVSEIVLFVLRPELIPGGPKSNIIVRFLIFVWVGIGAT
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCH
VGATATRILLNLAVEKQKEAKKVLDNLLEMAKTILNTIDLMKQQIKNQDTISEELKQISE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
HQASSLFEISTSLQELSSKADSNNKIAKSLYQESETSIQSVNDLKAINETVQTGTGSIYK
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
NLDVVTEYSNDTSKHIHLSIDKFNILQEKSREISEFVSVINDIADKVNLLSLNAAIEAAR
CHHHHEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGEHGRGFAVVAEEISKLALATTKNSKEISKIIKENLSLIGESSELINRSSEMMGKLDTS
CCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
IGIIKNEITGVGSKIVEIDKAIQTIDNLNTRIYETSRTIETSTNFQKVATEESTKITANI
HHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHEEECCCCEECCCCHHHHHCCCCCEEEEEH
SEYATNIVQISKQISESSKSTGGIISKLDSMAKEMTN
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7908398; 10984043 [H]