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The map label for this gene is pleD [H]

Identifier: 183220482

GI number: 183220482

Start: 1128870

End: 1129736

Strand: Reverse

Name: pleD [H]

Synonym: LEPBI_I1081

Alternate gene names: 183220482

Gene position: 1129736-1128870 (Counterclockwise)

Preceding gene: 183220483

Following gene: 183220476

Centisome position: 31.38

GC content: 35.99

Gene sequence:

>867_bases
GTGCCAGCTACATTTGATCTGATATGGTTAACTGTTCTTGCATTTGGAACTGGACATATTACTTCTTTTTTCATTTTAGG
ATATCTTGGATCCATTGCACTTAGTAGTATGTCTCTCGATAGAAACTACGGAATATACAATGCTTTTCTTGCTACTGTTT
TGTTTAGTGCGATGGGATTTTTGGTTTATTTTGAAATTTTGCCTTCAGTAAATATATTAATGCCTACAGTGAAACCCACT
TTAATGAGTATATTCATTTCTTCAATTTTGTTAGGTGGGAGTGCCTTCATCGTAAACCAAATTGTATCCAAATTGTTTTC
TTCATTAACTGATGTGAATCAAAAATTATTGGCGATCGCAATGACAGATCCATTGACTGGGATTTCCAATCGTAGATCAT
TTTTTTCAAATTTAGAAATAGAACTCGCAAGAAAACAAAGAAGTGTAGGTCCATATCCAATTGCATTCTTACTATTTGAT
TTAGATCTATTTAAATCCATCAATGATACTTATGGACATGAATTTGGAGATCAGGTTTTAATTGAATTTGCAACTGTTCT
CAAAACTTCACTACGCAAACAAGACTTCCCTGCTCGATGGGGAGGAGAAGAATTCCTGGTCCTCTTACCAAATACTGACC
TAGACGGCGCGATCGTCGTCGCTGAAAAAATTCGAACAAGCTTTCATTCCTTATCCTTTTATGCCAAAGATAAACGAATC
CAATGCTCCACGAGTGTAGGAATCTCTGTATGCAACGAACAAGGGATCAACCCAGAAGTGGTGATCAATCAAGCGGATCA
ATATTTATATGAAGCCAAACGAAATGGGAGAAACCAAATCTATTCGGAAAAAAATGTTCCCTCTTAA

Upstream 100 bases:

>100_bases
AGTCGCTATTGGCTCCATTCATCTAGTGTTTGCGATTGTTTGGTTTTTGTGAATTCACTATGATCTGTTAGTTGAAGAGG
AGATTTGGTGGAGTGGGTAT

Downstream 100 bases:

>100_bases
AAGATTACAACTGGATACAAAATTCCAATGAATCCTGATAGATCTGAATGTTTACCCGCTCACAAAAAAAGAAAAATTCA
GCTCGATGCCCGAAATGTGA

Product: hypothetical protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MPATFDLIWLTVLAFGTGHITSFFILGYLGSIALSSMSLDRNYGIYNAFLATVLFSAMGFLVYFEILPSVNILMPTVKPT
LMSIFISSILLGGSAFIVNQIVSKLFSSLTDVNQKLLAIAMTDPLTGISNRRSFFSNLEIELARKQRSVGPYPIAFLLFD
LDLFKSINDTYGHEFGDQVLIEFATVLKTSLRKQDFPARWGGEEFLVLLPNTDLDGAIVVAEKIRTSFHSLSFYAKDKRI
QCSTSVGISVCNEQGINPEVVINQADQYLYEAKRNGRNQIYSEKNVPS

Sequences:

>Translated_288_residues
MPATFDLIWLTVLAFGTGHITSFFILGYLGSIALSSMSLDRNYGIYNAFLATVLFSAMGFLVYFEILPSVNILMPTVKPT
LMSIFISSILLGGSAFIVNQIVSKLFSSLTDVNQKLLAIAMTDPLTGISNRRSFFSNLEIELARKQRSVGPYPIAFLLFD
LDLFKSINDTYGHEFGDQVLIEFATVLKTSLRKQDFPARWGGEEFLVLLPNTDLDGAIVVAEKIRTSFHSLSFYAKDKRI
QCSTSVGISVCNEQGINPEVVINQADQYLYEAKRNGRNQIYSEKNVPS
>Mature_287_residues
PATFDLIWLTVLAFGTGHITSFFILGYLGSIALSSMSLDRNYGIYNAFLATVLFSAMGFLVYFEILPSVNILMPTVKPTL
MSIFISSILLGGSAFIVNQIVSKLFSSLTDVNQKLLAIAMTDPLTGISNRRSFFSNLEIELARKQRSVGPYPIAFLLFDL
DLFKSINDTYGHEFGDQVLIEFATVLKTSLRKQDFPARWGGEEFLVLLPNTDLDGAIVVAEKIRTSFHSLSFYAKDKRIQ
CSTSVGISVCNEQGINPEVVINQADQYLYEAKRNGRNQIYSEKNVPS

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=166, Percent_Identity=39.7590361445783, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI87082007, Length=234, Percent_Identity=34.6153846153846, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI87081881, Length=170, Percent_Identity=37.6470588235294, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1786584, Length=173, Percent_Identity=30.635838150289, Blast_Score=97, Evalue=9e-22,
Organism=Escherichia coli, GI1787541, Length=217, Percent_Identity=31.7972350230415, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788381, Length=161, Percent_Identity=32.9192546583851, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI145693134, Length=184, Percent_Identity=32.0652173913043, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1788085, Length=187, Percent_Identity=32.0855614973262, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1787816, Length=230, Percent_Identity=28.695652173913, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787802, Length=179, Percent_Identity=30.1675977653631, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787056, Length=180, Percent_Identity=36.1111111111111, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87081977, Length=191, Percent_Identity=30.8900523560209, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788956, Length=165, Percent_Identity=32.7272727272727, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI87081974, Length=155, Percent_Identity=30.9677419354839, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 32063; Mature: 31932

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPATFDLIWLTVLAFGTGHITSFFILGYLGSIALSSMSLDRNYGIYNAFLATVLFSAMGF
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
LVYFEILPSVNILMPTVKPTLMSIFISSILLGGSAFIVNQIVSKLFSSLTDVNQKLLAIA
HHHHHHHCCCCEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEEE
MTDPLTGISNRRSFFSNLEIELARKQRSVGPYPIAFLLFDLDLFKSINDTYGHEFGDQVL
ECCCCCCCHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHH
IEFATVLKTSLRKQDFPARWGGEEFLVLLPNTDLDGAIVVAEKIRTSFHSLSFYAKDKRI
HHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHEECCCEE
QCSTSVGISVCNEQGINPEVVINQADQYLYEAKRNGRNQIYSEKNVPS
EEECCCCCEECCCCCCCHHEEEEHHHHHHHHHHHCCCHHCCCCCCCCC
>Mature Secondary Structure 
PATFDLIWLTVLAFGTGHITSFFILGYLGSIALSSMSLDRNYGIYNAFLATVLFSAMGF
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
LVYFEILPSVNILMPTVKPTLMSIFISSILLGGSAFIVNQIVSKLFSSLTDVNQKLLAIA
HHHHHHHCCCCEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEEE
MTDPLTGISNRRSFFSNLEIELARKQRSVGPYPIAFLLFDLDLFKSINDTYGHEFGDQVL
ECCCCCCCHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHH
IEFATVLKTSLRKQDFPARWGGEEFLVLLPNTDLDGAIVVAEKIRTSFHSLSFYAKDKRI
HHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHEECCCEE
QCSTSVGISVCNEQGINPEVVINQADQYLYEAKRNGRNQIYSEKNVPS
EEECCCCCEECCCCCCCHHEEEEHHHHHHHHHHHCCCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA