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The map label for this gene is rlmL [H]

Identifier: 183220444

GI number: 183220444

Start: 1081311

End: 1082147

Strand: Reverse

Name: rlmL [H]

Synonym: LEPBI_I1041

Alternate gene names: 183220444

Gene position: 1082147-1081311 (Counterclockwise)

Preceding gene: 183220445

Following gene: 183220443

Centisome position: 30.06

GC content: 43.85

Gene sequence:

>837_bases
ATGACAAAAAGTTACGAATTATTGGATTCTGGGGACCTTTCCAAATTAGAAATTGTGGGCGGATACAAACTCCAAAGGTC
CTCCCCTACTTCCGCCTATGGCAAAGAAACACCTGGGATCTGGAACGACTTACATGCTGTTTACATTAAAAATGATTCTG
GCTCAGGGCATTGGAACTTCCAAAAAAAAGTCCCAGAAAGTTTCACGATTCAATTTTCCCATCTTACCTTTAAAATCAAA
CTCACACCATTTGGGCATATTGGACTCTTCCCCGAACAAGAAACCAACTGGAACCGAATCCGAGAAATCGGGAAAAGAAA
ACAAGGCCTGGAAGTTTTGAATTTATTTGCGTATTCTGGTGGATCCACTCTCGCTTGCCTTGATGCCGGGATGAGTGTTT
GCCATGTAGATGCTTCCAAAGGGATGGTGGATTGGGCGAGAGAAAATGCCAAACTCTCAGGTCTAGATACCAAACCCGTT
CGTTGGATCGTAGATGATGTGATGAAATTCATACGTCGCGAAATCAAACGTGGGAAAAAATACCAAGGCCTCATCCTCGA
CCCGCCAAGTTTCGGTCGTGGTTCCAAAGGAGAGGTTTGGAAAATTGAAGAGAACCTACCCGAACTCATGGATGCACTGA
TGGAACTCTCCGACAACAAACCTGAATTTGTCATCCTCAGTTGCCATAGCCAAGGGTTTAGTCCCCTCACGTTAGAAAGG
ATCCTCTCCTCACGGATCAAAACAAAAGGAACATACGAAACAACTGAACTTTATATCCCAGAAACTTCTGGGAAAAAATA
TCCGGCTGGATTCTGCACCTTTTTCAAACGTTCCTAA

Upstream 100 bases:

>100_bases
ATTTATTCAAAGGAAAACTCATCCAAACTTTGGAGTCCTTTTCAAATCGAGAAATAAAAGACTGGGAGGACGACCTAACC
TTTATTTTTCTGGAATTGGT

Downstream 100 bases:

>100_bases
TCGAGTATTTCTCCAATGAAACCACAAAGGAATTACATCACAAGTTTCTCCAATCCGAAGGTGAAATGGGTAGCAGGCCT
CAAAGAAAAACGAAATCGTG

Product: putative SAM dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MTKSYELLDSGDLSKLEIVGGYKLQRSSPTSAYGKETPGIWNDLHAVYIKNDSGSGHWNFQKKVPESFTIQFSHLTFKIK
LTPFGHIGLFPEQETNWNRIREIGKRKQGLEVLNLFAYSGGSTLACLDAGMSVCHVDASKGMVDWARENAKLSGLDTKPV
RWIVDDVMKFIRREIKRGKKYQGLILDPPSFGRGSKGEVWKIEENLPELMDALMELSDNKPEFVILSCHSQGFSPLTLER
ILSSRIKTKGTYETTELYIPETSGKKYPAGFCTFFKRS

Sequences:

>Translated_278_residues
MTKSYELLDSGDLSKLEIVGGYKLQRSSPTSAYGKETPGIWNDLHAVYIKNDSGSGHWNFQKKVPESFTIQFSHLTFKIK
LTPFGHIGLFPEQETNWNRIREIGKRKQGLEVLNLFAYSGGSTLACLDAGMSVCHVDASKGMVDWARENAKLSGLDTKPV
RWIVDDVMKFIRREIKRGKKYQGLILDPPSFGRGSKGEVWKIEENLPELMDALMELSDNKPEFVILSCHSQGFSPLTLER
ILSSRIKTKGTYETTELYIPETSGKKYPAGFCTFFKRS
>Mature_277_residues
TKSYELLDSGDLSKLEIVGGYKLQRSSPTSAYGKETPGIWNDLHAVYIKNDSGSGHWNFQKKVPESFTIQFSHLTFKIKL
TPFGHIGLFPEQETNWNRIREIGKRKQGLEVLNLFAYSGGSTLACLDAGMSVCHVDASKGMVDWARENAKLSGLDTKPVR
WIVDDVMKFIRREIKRGKKYQGLILDPPSFGRGSKGEVWKIEENLPELMDALMELSDNKPEFVILSCHSQGFSPLTLERI
LSSRIKTKGTYETTELYIPETSGKKYPAGFCTFFKRS

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=81, Percent_Identity=40.7407407407407, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 31415; Mature: 31284

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKSYELLDSGDLSKLEIVGGYKLQRSSPTSAYGKETPGIWNDLHAVYIKNDSGSGHWNF
CCCCCCCCCCCCCCEEEEEECEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEE
QKKVPESFTIQFSHLTFKIKLTPFGHIGLFPEQETNWNRIREIGKRKQGLEVLNLFAYSG
HHCCCCEEEEEEEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCHHEEEEEEECC
GSTLACLDAGMSVCHVDASKGMVDWARENAKLSGLDTKPVRWIVDDVMKFIRREIKRGKK
CCEEEEECCCCEEEEEECCCCHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHCCCC
YQGLILDPPSFGRGSKGEVWKIEENLPELMDALMELSDNKPEFVILSCHSQGFSPLTLER
CCCEEECCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHH
ILSSRIKTKGTYETTELYIPETSGKKYPAGFCTFFKRS
HHHHHHCCCCCEEEEEEEEECCCCCCCCCHHHHHCCCC
>Mature Secondary Structure 
TKSYELLDSGDLSKLEIVGGYKLQRSSPTSAYGKETPGIWNDLHAVYIKNDSGSGHWNF
CCCCCCCCCCCCCEEEEEECEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEE
QKKVPESFTIQFSHLTFKIKLTPFGHIGLFPEQETNWNRIREIGKRKQGLEVLNLFAYSG
HHCCCCEEEEEEEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCHHEEEEEEECC
GSTLACLDAGMSVCHVDASKGMVDWARENAKLSGLDTKPVRWIVDDVMKFIRREIKRGKK
CCEEEEECCCCEEEEEECCCCHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHCCCC
YQGLILDPPSFGRGSKGEVWKIEENLPELMDALMELSDNKPEFVILSCHSQGFSPLTLER
CCCEEECCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHH
ILSSRIKTKGTYETTELYIPETSGKKYPAGFCTFFKRS
HHHHHHCCCCCEEEEEEEEECCCCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA