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The map label for this gene is ruvB [H]

Identifier: 183219942

GI number: 183219942

Start: 522943

End: 523968

Strand: Direct

Name: ruvB [H]

Synonym: LEPBI_I0524

Alternate gene names: 183219942

Gene position: 522943-523968 (Clockwise)

Preceding gene: 183219941

Following gene: 183219943

Centisome position: 14.53

GC content: 48.93

Gene sequence:

>1026_bases
GTGAGTTTACGAGAAGATTGGATGCAAGCGGGCGAAGAAGAACCAAACCTTCGCCCCACAAAACTGTCTGAGTTCATTGG
ACAAAAAGAGGTCTTAGCCAATCTATCGGTGTATGTCGAGGCGGCTCGGAAACGAAAGAGCCCTCTTGACCACGTGCTCA
TTTCAGGCCCACCGGGCCTTGGAAAAACCACTCTCGCCAATATCATCGCCAACGAACTGGCCGTTGCCTTCACTCCTACA
TCAGCTCCCGCCATTTCCAAGGGAGCCGATTTGGTACGGTTTCTCACTCTACTCAAAACCAACGAAGTCCTCTTCATCGA
TGAAATCCATGGTTTCATCAAAAAACAGGAAGAACTGTTATACCCAGCCATGGAGAATTTTTTTGTGGATCTCGTTGTGG
GAGAAGGTGTCACCGCAAACGCATTACAAATCCAACTCCAACCCTTCACCTTAGTCGGTGCCACCACAAGGTCAGGTCTT
GTGAGTGATCCACTCAAATCTCGATTTGGCATCCATCTCAAGTTGGATTTTTATACGGATGAAGAGATGCAAATCATCGT
GGACCGATCGGCAAAACTTCTGGGAGTGGAACTCGGGCAAGGTGTGGCTATGGAAGTTGGCAAACGAAGTCGCAAAACGC
CAAGGATTGCCAACCACTTACTCAAACGGGTGCGTGATTTTGCGGAGGTAAATAACGAAACCTCTGTGAGTTTAAAGACT
TGTCGTTATGCCTTTGACCGCATGGGAGTCGACCATCTGGGCCTTGATGCCGTGGACCGCCAAATTTTAGATATCCTCAT
CTCTCGATATGGTGGAGGGCCTGTGGGGATCAAACCGATTGCCGTTGTACTTGGTGAGGAAGAACGCACCATTGAAGACA
CCTACGAACCTTTCCTTGTTCGGGTAGGGCTCATTGACCGCACTCCGCAAGGCCGAGTGGCTACGAAAAAGGCTTACGAA
CATTTAGGACTTCCGTACATTGGAAATACGGGAGAAAATCGTGAAAATGGCCCCACTCTCTTTTGA

Upstream 100 bases:

>100_bases
ATTGTGAATTCCAAAATTGGCAAACGAGTCGAACTAAAAATCATCCGAAATAAAAATGAAATCTTGACGTCCATCACTCC
AGAGAAGAAACCCAATTGAG

Downstream 100 bases:

>100_bases
TTTCCGATTGCCTGAAAAAGAAGAAGGGGAAAAACGCCTCTTCTTTGCCTTCACATTTGTGATCCTTCTTTCTTCCTTTT
TTTTGGCTCACCTCATCACG

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MSLREDWMQAGEEEPNLRPTKLSEFIGQKEVLANLSVYVEAARKRKSPLDHVLISGPPGLGKTTLANIIANELAVAFTPT
SAPAISKGADLVRFLTLLKTNEVLFIDEIHGFIKKQEELLYPAMENFFVDLVVGEGVTANALQIQLQPFTLVGATTRSGL
VSDPLKSRFGIHLKLDFYTDEEMQIIVDRSAKLLGVELGQGVAMEVGKRSRKTPRIANHLLKRVRDFAEVNNETSVSLKT
CRYAFDRMGVDHLGLDAVDRQILDILISRYGGGPVGIKPIAVVLGEEERTIEDTYEPFLVRVGLIDRTPQGRVATKKAYE
HLGLPYIGNTGENRENGPTLF

Sequences:

>Translated_341_residues
MSLREDWMQAGEEEPNLRPTKLSEFIGQKEVLANLSVYVEAARKRKSPLDHVLISGPPGLGKTTLANIIANELAVAFTPT
SAPAISKGADLVRFLTLLKTNEVLFIDEIHGFIKKQEELLYPAMENFFVDLVVGEGVTANALQIQLQPFTLVGATTRSGL
VSDPLKSRFGIHLKLDFYTDEEMQIIVDRSAKLLGVELGQGVAMEVGKRSRKTPRIANHLLKRVRDFAEVNNETSVSLKT
CRYAFDRMGVDHLGLDAVDRQILDILISRYGGGPVGIKPIAVVLGEEERTIEDTYEPFLVRVGLIDRTPQGRVATKKAYE
HLGLPYIGNTGENRENGPTLF
>Mature_340_residues
SLREDWMQAGEEEPNLRPTKLSEFIGQKEVLANLSVYVEAARKRKSPLDHVLISGPPGLGKTTLANIIANELAVAFTPTS
APAISKGADLVRFLTLLKTNEVLFIDEIHGFIKKQEELLYPAMENFFVDLVVGEGVTANALQIQLQPFTLVGATTRSGLV
SDPLKSRFGIHLKLDFYTDEEMQIIVDRSAKLLGVELGQGVAMEVGKRSRKTPRIANHLLKRVRDFAEVNNETSVSLKTC
RYAFDRMGVDHLGLDAVDRQILDILISRYGGGPVGIKPIAVVLGEEERTIEDTYEPFLVRVGLIDRTPQGRVATKKAYEH
LGLPYIGNTGENRENGPTLF

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family [H]

Homologues:

Organism=Escherichia coli, GI1788167, Length=308, Percent_Identity=48.7012987012987, Blast_Score=318, Evalue=3e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- ProDom:   PD005323 [H]

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 37724; Mature: 37593

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLREDWMQAGEEEPNLRPTKLSEFIGQKEVLANLSVYVEAARKRKSPLDHVLISGPPGL
CCCHHHHHHCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
GKTTLANIIANELAVAFTPTSAPAISKGADLVRFLTLLKTNEVLFIDEIHGFIKKQEELL
CHHHHHHHHHHHHEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHH
YPAMENFFVDLVVGEGVTANALQIQLQPFTLVGATTRSGLVSDPLKSRFGIHLKLDFYTD
HHHHHHHEEEEEECCCCCCCEEEEEEECEEEEECCCCCCCCCCCHHHHCCEEEEEEEECC
EEMQIIVDRSAKLLGVELGQGVAMEVGKRSRKTPRIANHLLKRVRDFAEVNNETSVSLKT
CCEEEEEECCCEEEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHH
CRYAFDRMGVDHLGLDAVDRQILDILISRYGGGPVGIKPIAVVLGEEERTIEDTYEPFLV
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHCCCEEE
RVGLIDRTPQGRVATKKAYEHLGLPYIGNTGENRENGPTLF
EEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SLREDWMQAGEEEPNLRPTKLSEFIGQKEVLANLSVYVEAARKRKSPLDHVLISGPPGL
CCHHHHHHCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
GKTTLANIIANELAVAFTPTSAPAISKGADLVRFLTLLKTNEVLFIDEIHGFIKKQEELL
CHHHHHHHHHHHHEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHH
YPAMENFFVDLVVGEGVTANALQIQLQPFTLVGATTRSGLVSDPLKSRFGIHLKLDFYTD
HHHHHHHEEEEEECCCCCCCEEEEEEECEEEEECCCCCCCCCCCHHHHCCEEEEEEEECC
EEMQIIVDRSAKLLGVELGQGVAMEVGKRSRKTPRIANHLLKRVRDFAEVNNETSVSLKT
CCEEEEEECCCEEEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHH
CRYAFDRMGVDHLGLDAVDRQILDILISRYGGGPVGIKPIAVVLGEEERTIEDTYEPFLV
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHCCCEEE
RVGLIDRTPQGRVATKKAYEHLGLPYIGNTGENRENGPTLF
EEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA