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The map label for this gene is recG [H]

Identifier: 183219772

GI number: 183219772

Start: 347933

End: 349993

Strand: Direct

Name: recG [H]

Synonym: LEPBI_I0349

Alternate gene names: 183219772

Gene position: 347933-349993 (Clockwise)

Preceding gene: 183219771

Following gene: 183219773

Centisome position: 9.67

GC content: 38.86

Gene sequence:

>2061_bases
ATGTTTGATGTCACACAAAGTTTATCGAATCTCAAAGGGATTGGTCCAAAACGAAAAACTGTATTATTAGAACATGGAAT
CTCTACGTATTATGAATTATTAACCTATTTTCCAAGACGATACTTGGATCGTAATTTTACAAAAGATATCATTTTAAAAC
AAGGTGATGTAGTCACTTTACTTGGAAGTATTGTAGATAGTTACATTGTGCACGGAAAAAAAAGTCGGTTACTTGTTGGC
TTTCGAACCTTAAACAATGAAAGGATCAATTTAGTTTTCTTTCGTGGAGTCAATTTTTTTCAAAAACTCTTTGCCATCGA
TAAAAAGGTTGTGGTCTCAGGGAAATTAGAATACTTTCGCGGTTACCAAATCCTCCATCCTGAATATGAATTTTTATCCG
ATGCAGATGACCCAGAAGATTCCATTCACGCTGGGCGCATCATTCCCTTATACCCTTCCACGGAAGCATTAAAAGAAGAT
GGACTCGATTCCAAGGGTTTACGGAAACTCATCCACCAAGTTTTGGAAGCTGGAACAATCAGCGAAAATTTGCCATCCAA
ATTCATCAAAAAACGTAAGTTACTTGGTCGTGACGAAGCCTTTCGAAACATCCACTTTCCTGACACAATGGAAACTGTCC
AAATAGCTCGGAAACGGTTTGCGTATGAGGAATTTTATTACTTCCAACGTTTGCTTTTGTACAAACAAAGAGAGAGACAA
AAAGTAAAACGACTGTTATGGCCACTTCCAAAATCTCCATCACGAACTAATTTAGAAAAAAATCTTCCGTTTGAACTCAC
AGAAGACCAAAAATCGGCTGTCACAACCATCCTTTCTAAAACCAATTCCGATTCGCCTTCTGCATTTTTATTACAAGGGG
ATGTTGGTTCTGGAAAAACCATCACGGCTCTCCTTGTTGGTTTGCATTATATTGACAACCACATCCAAGTTGTGTTTCTC
GCACCAACGGAAATCCTAGCTAGACAACATTACCAAACGATTTATAAATTTATGGGAAACATGCCATTCCTTGGGATCGA
ATTATTGTTAGGTGGCGAGAATAAAAAAACCAGAGCTGAAAAACTATCTAGGATCAAAACGGGAGAATCCAATATCATCA
TTGGAACACATTCTTTATTACAAGAAGATGTGATCTTTTCTGATTTAGGACTCGTTGTGATCGATGAACAACATAAGTTT
GGAGTGGACCAAAGGGAAACCATACGTGCCAAAGGAAAAAATCCTGATATATTGGCCATGACTGCCACACCCATCCCTCG
GACACTTTGCCTCACATTGTATGGTGATTTAACTCTCGTGAATATCAAAACAAAACCAAAAGGTCGAAAACCCATTGATA
CACGTTGGTACAAAGAAGACCGTAGGGCCGGTGTTTACAACTCTATCCGAAAGTATGTTAGTTCAGGTAGACAATGTTAC
ATTGTGTACCCGCTTGTAGAAGAATCCGAAAAAGTGGATTTAGAATCTTGTACAGTGGCTTATGAAAATTTGAGAACAAC
GGTTTTTCCTGACTTAAAGATTGGCTTATTACATGGAAAAATGAAAAGTGTCGAAAAAGAATCCGTGATGGAAAAATTCA
AGTCAGGGGAAATCCAAATCCTTGTCACAACAACTGTTGTGGAAGTGGGTGTGGATGTACCCAATGCTACCATTTTAGTT
GTGGAACATGCAGAACGATTTGGAATTTCCCAATTACACCAGTTACGTGGTCGTGTTGGTAGAAGTGATTTGGAAAGTTT
TTGTATTCTGATGACGGGTGATTCTATCAGCGAAGAAGGTAGGGACCGTCTCGAAGCCCTTGTCGCTTCCAATGATGGAT
ATTATTTGGCAGAGAAAGACCTTGCCATTCGCGGTCCTGGAGAACTCTTAGGAGTCAAACAAAGTGGATTACCTGAGTTT
AAAATCGCAGATTTAGTCGTGGACAGAGAACTTTTGGATGAGGCGAAAGAAGATGCATCTTCCATCCCACTTGATGACCC
AAATGAAGTATCTGAGTTAAGGATTCGATTTAGCGAAGGTAAATTTTTATTTGCGAATTAA

Upstream 100 bases:

>100_bases
GTTATCTCATTTTAGATTCCGAAAAAAACAGTACTGTTCCAAACAATACAAATGACAATCCCTTTGGCCAACCGAAACCA
ACCGATGAGATATAAAATTT

Downstream 100 bases:

>100_bases
CCAAAGATTTTTAAATGAACCTTATTATGTTCAGACCAAATTATCTTTTTTTGTTTTTGCTCTCATTATTATGTGTCTGT
GGTGAAAAACCAGTCAAACA

Product: ATP-dependent DNA helicase RecG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 686; Mature: 686

Protein sequence:

>686_residues
MFDVTQSLSNLKGIGPKRKTVLLEHGISTYYELLTYFPRRYLDRNFTKDIILKQGDVVTLLGSIVDSYIVHGKKSRLLVG
FRTLNNERINLVFFRGVNFFQKLFAIDKKVVVSGKLEYFRGYQILHPEYEFLSDADDPEDSIHAGRIIPLYPSTEALKED
GLDSKGLRKLIHQVLEAGTISENLPSKFIKKRKLLGRDEAFRNIHFPDTMETVQIARKRFAYEEFYYFQRLLLYKQRERQ
KVKRLLWPLPKSPSRTNLEKNLPFELTEDQKSAVTTILSKTNSDSPSAFLLQGDVGSGKTITALLVGLHYIDNHIQVVFL
APTEILARQHYQTIYKFMGNMPFLGIELLLGGENKKTRAEKLSRIKTGESNIIIGTHSLLQEDVIFSDLGLVVIDEQHKF
GVDQRETIRAKGKNPDILAMTATPIPRTLCLTLYGDLTLVNIKTKPKGRKPIDTRWYKEDRRAGVYNSIRKYVSSGRQCY
IVYPLVEESEKVDLESCTVAYENLRTTVFPDLKIGLLHGKMKSVEKESVMEKFKSGEIQILVTTTVVEVGVDVPNATILV
VEHAERFGISQLHQLRGRVGRSDLESFCILMTGDSISEEGRDRLEALVASNDGYYLAEKDLAIRGPGELLGVKQSGLPEF
KIADLVVDRELLDEAKEDASSIPLDDPNEVSELRIRFSEGKFLFAN

Sequences:

>Translated_686_residues
MFDVTQSLSNLKGIGPKRKTVLLEHGISTYYELLTYFPRRYLDRNFTKDIILKQGDVVTLLGSIVDSYIVHGKKSRLLVG
FRTLNNERINLVFFRGVNFFQKLFAIDKKVVVSGKLEYFRGYQILHPEYEFLSDADDPEDSIHAGRIIPLYPSTEALKED
GLDSKGLRKLIHQVLEAGTISENLPSKFIKKRKLLGRDEAFRNIHFPDTMETVQIARKRFAYEEFYYFQRLLLYKQRERQ
KVKRLLWPLPKSPSRTNLEKNLPFELTEDQKSAVTTILSKTNSDSPSAFLLQGDVGSGKTITALLVGLHYIDNHIQVVFL
APTEILARQHYQTIYKFMGNMPFLGIELLLGGENKKTRAEKLSRIKTGESNIIIGTHSLLQEDVIFSDLGLVVIDEQHKF
GVDQRETIRAKGKNPDILAMTATPIPRTLCLTLYGDLTLVNIKTKPKGRKPIDTRWYKEDRRAGVYNSIRKYVSSGRQCY
IVYPLVEESEKVDLESCTVAYENLRTTVFPDLKIGLLHGKMKSVEKESVMEKFKSGEIQILVTTTVVEVGVDVPNATILV
VEHAERFGISQLHQLRGRVGRSDLESFCILMTGDSISEEGRDRLEALVASNDGYYLAEKDLAIRGPGELLGVKQSGLPEF
KIADLVVDRELLDEAKEDASSIPLDDPNEVSELRIRFSEGKFLFAN
>Mature_686_residues
MFDVTQSLSNLKGIGPKRKTVLLEHGISTYYELLTYFPRRYLDRNFTKDIILKQGDVVTLLGSIVDSYIVHGKKSRLLVG
FRTLNNERINLVFFRGVNFFQKLFAIDKKVVVSGKLEYFRGYQILHPEYEFLSDADDPEDSIHAGRIIPLYPSTEALKED
GLDSKGLRKLIHQVLEAGTISENLPSKFIKKRKLLGRDEAFRNIHFPDTMETVQIARKRFAYEEFYYFQRLLLYKQRERQ
KVKRLLWPLPKSPSRTNLEKNLPFELTEDQKSAVTTILSKTNSDSPSAFLLQGDVGSGKTITALLVGLHYIDNHIQVVFL
APTEILARQHYQTIYKFMGNMPFLGIELLLGGENKKTRAEKLSRIKTGESNIIIGTHSLLQEDVIFSDLGLVVIDEQHKF
GVDQRETIRAKGKNPDILAMTATPIPRTLCLTLYGDLTLVNIKTKPKGRKPIDTRWYKEDRRAGVYNSIRKYVSSGRQCY
IVYPLVEESEKVDLESCTVAYENLRTTVFPDLKIGLLHGKMKSVEKESVMEKFKSGEIQILVTTTVVEVGVDVPNATILV
VEHAERFGISQLHQLRGRVGRSDLESFCILMTGDSISEEGRDRLEALVASNDGYYLAEKDLAIRGPGELLGVKQSGLPEF
KIADLVVDRELLDEAKEDASSIPLDDPNEVSELRIRFSEGKFLFAN

Specific function: Critical role in recombination and DNA repair. Help process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3' to 5' polarity. RecG unwind branched dup

COG id: COG1200

COG function: function code LK; RecG-like helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367254, Length=690, Percent_Identity=36.9565217391304, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI1787357, Length=381, Percent_Identity=40.1574803149606, Blast_Score=268, Evalue=7e-73,
Organism=Escherichia coli, GI1790370, Length=179, Percent_Identity=34.0782122905028, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR004609
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C; PF01336 tRNA_anti [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 77953; Mature: 77953

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDVTQSLSNLKGIGPKRKTVLLEHGISTYYELLTYFPRRYLDRNFTKDIILKQGDVVTL
CCCHHHHHHHHCCCCCCHHEEEEHHCHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCHHHH
LGSIVDSYIVHGKKSRLLVGFRTLNNERINLVFFRGVNFFQKLFAIDKKVVVSGKLEYFR
HHHHHHHHHHCCCCCEEEEEEEECCCCEEEEEEECCHHHHHHHHHHCCCEEEECCHHHHC
GYQILHPEYEFLSDADDPEDSIHAGRIIPLYPSTEALKEDGLDSKGLRKLIHQVLEAGTI
CEEEECCCHHHHCCCCCCCCCCCCCCEEEECCCHHHHHHCCCCHHHHHHHHHHHHHCCCC
SENLPSKFIKKRKLLGRDEAFRNIHFPDTMETVQIARKRFAYEEFYYFQRLLLYKQRERQ
CCCCHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KVKRLLWPLPKSPSRTNLEKNLPFELTEDQKSAVTTILSKTNSDSPSAFLLQGDVGSGKT
HHHHHHCCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCH
ITALLVGLHYIDNHIQVVFLAPTEILARQHYQTIYKFMGNMPFLGIELLLGGENKKTRAE
HHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHH
KLSRIKTGESNIIIGTHSLLQEDVIFSDLGLVVIDEQHKFGVDQRETIRAKGKNPDILAM
HHHHHCCCCCCEEEEEHHHHHHHHHHHCCCEEEEECCHHCCCCHHHHHHCCCCCCCEEEE
TATPIPRTLCLTLYGDLTLVNIKTKPKGRKPIDTRWYKEDRRAGVYNSIRKYVSSGRQCY
ECCCCCHHHHEEEECCEEEEEEEECCCCCCCCCCHHHHHHHCCCHHHHHHHHHHCCCEEE
IVYPLVEESEKVDLESCTVAYENLRTTVFPDLKIGLLHGKMKSVEKESVMEKFKSGEIQI
EEEEEECCCCCCCHHHHHHHHHHCCEEECCCCEEEEEHHHHHHHHHHHHHHHHCCCCEEE
LVTTTVVEVGVDVPNATILVVEHAERFGISQLHQLRGRVGRSDLESFCILMTGDSISEEG
EEEEEEEEECCCCCCCEEEEEECHHHCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHH
RDRLEALVASNDGYYLAEKDLAIRGPGELLGVKQSGLPEFKIADLVVDRELLDEAKEDAS
HHHHHHHHCCCCCEEEEECCEEEECCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHC
SIPLDDPNEVSELRIRFSEGKFLFAN
CCCCCCCCCHHHEEEEEECCCEEECC
>Mature Secondary Structure
MFDVTQSLSNLKGIGPKRKTVLLEHGISTYYELLTYFPRRYLDRNFTKDIILKQGDVVTL
CCCHHHHHHHHCCCCCCHHEEEEHHCHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCHHHH
LGSIVDSYIVHGKKSRLLVGFRTLNNERINLVFFRGVNFFQKLFAIDKKVVVSGKLEYFR
HHHHHHHHHHCCCCCEEEEEEEECCCCEEEEEEECCHHHHHHHHHHCCCEEEECCHHHHC
GYQILHPEYEFLSDADDPEDSIHAGRIIPLYPSTEALKEDGLDSKGLRKLIHQVLEAGTI
CEEEECCCHHHHCCCCCCCCCCCCCCEEEECCCHHHHHHCCCCHHHHHHHHHHHHHCCCC
SENLPSKFIKKRKLLGRDEAFRNIHFPDTMETVQIARKRFAYEEFYYFQRLLLYKQRERQ
CCCCHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KVKRLLWPLPKSPSRTNLEKNLPFELTEDQKSAVTTILSKTNSDSPSAFLLQGDVGSGKT
HHHHHHCCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCH
ITALLVGLHYIDNHIQVVFLAPTEILARQHYQTIYKFMGNMPFLGIELLLGGENKKTRAE
HHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHH
KLSRIKTGESNIIIGTHSLLQEDVIFSDLGLVVIDEQHKFGVDQRETIRAKGKNPDILAM
HHHHHCCCCCCEEEEEHHHHHHHHHHHCCCEEEEECCHHCCCCHHHHHHCCCCCCCEEEE
TATPIPRTLCLTLYGDLTLVNIKTKPKGRKPIDTRWYKEDRRAGVYNSIRKYVSSGRQCY
ECCCCCHHHHEEEECCEEEEEEEECCCCCCCCCCHHHHHHHCCCHHHHHHHHHHCCCEEE
IVYPLVEESEKVDLESCTVAYENLRTTVFPDLKIGLLHGKMKSVEKESVMEKFKSGEIQI
EEEEEECCCCCCCHHHHHHHHHHCCEEECCCCEEEEEHHHHHHHHHHHHHHHHCCCCEEE
LVTTTVVEVGVDVPNATILVVEHAERFGISQLHQLRGRVGRSDLESFCILMTGDSISEEG
EEEEEEEEECCCCCCCEEEEEECHHHCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHH
RDRLEALVASNDGYYLAEKDLAIRGPGELLGVKQSGLPEFKIADLVVDRELLDEAKEDAS
HHHHHHHHCCCCCEEEEECCEEEECCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHC
SIPLDDPNEVSELRIRFSEGKFLFAN
CCCCCCCCCHHHEEEEEECCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]