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The map label for this gene is ispF

Identifier: 183219746

GI number: 183219746

Start: 321874

End: 322362

Strand: Direct

Name: ispF

Synonym: LEPBI_I0322

Alternate gene names: 183219746

Gene position: 321874-322362 (Clockwise)

Preceding gene: 183219745

Following gene: 183219748

Centisome position: 8.94

GC content: 43.97

Gene sequence:

>489_bases
ATGTTTAGAGTCGGAAACGGAATCGATTTTCATAAATTAATCCATGAACCCTTTCGCCCCCTTGTGCTTGCGGGAGTTGA
GATTAAATCAGAGTTTGCCTTTCTCGGTCATAGTGATGCTGATGTGATTTTACATGCAGTGGCGGATGCGATTTTGGGTG
CATTGTCACTCGGAGATATTGGCGTCCATTTCCCGGACACTGACCCGCAATACAAAAATATGAAATCCACTCGCATCATC
GAAAAATGTTTGGAACTTGTGGCTGAGAAAAAATTCAAACTGATCAATGTGGACTGCACCTATGTGGGAGACCATCCAAA
GATAAGTCCGATCCGAGCAGAACTCAATGCTTCCCTTGCAAATATCACCAAACTTCCGTTAGATTGTGTTTCAATCAAAG
CAACCACCTCGGAAGGAATGGGAGCTTTGGGGAGAAGTGAGGGTGTGATGGTGATGGCAACTGTGCTCATCGAGAGTTTA
AAAAAGTAA

Upstream 100 bases:

>100_bases
CTTTTGAAGGAACAGTTTGAAATCTTTTTGGACGAGGAAGTGATTCGCCTTGCCCAAAAATCTGTGAATCGTATGTTAGA
GTTGAGTTATAAAAAATAAT

Downstream 100 bases:

>100_bases
GTTTAGAATCGTTTTTCTTTCGAAGTGGCGATGTGATGTAGATCCGCCTTGCCAAAGAATAAAAATAAAAGTTTAGAAAG
GAAATTGGGAGCCGGTTGGA

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MFRVGNGIDFHKLIHEPFRPLVLAGVEIKSEFAFLGHSDADVILHAVADAILGALSLGDIGVHFPDTDPQYKNMKSTRII
EKCLELVAEKKFKLINVDCTYVGDHPKISPIRAELNASLANITKLPLDCVSIKATTSEGMGALGRSEGVMVMATVLIESL
KK

Sequences:

>Translated_162_residues
MFRVGNGIDFHKLIHEPFRPLVLAGVEIKSEFAFLGHSDADVILHAVADAILGALSLGDIGVHFPDTDPQYKNMKSTRII
EKCLELVAEKKFKLINVDCTYVGDHPKISPIRAELNASLANITKLPLDCVSIKATTSEGMGALGRSEGVMVMATVLIESL
KK
>Mature_162_residues
MFRVGNGIDFHKLIHEPFRPLVLAGVEIKSEFAFLGHSDADVILHAVADAILGALSLGDIGVHFPDTDPQYKNMKSTRII
EKCLELVAEKKFKLINVDCTYVGDHPKISPIRAELNASLANITKLPLDCVSIKATTSEGMGALGRSEGVMVMATVLIESL
KK

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=156, Percent_Identity=39.7435897435897, Blast_Score=106, Evalue=6e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_LEPBA (B0SAT2)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001961437.1
- ProteinModelPortal:   B0SAT2
- SMR:   B0SAT2
- GeneID:   6386831
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_0313
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- ProtClustDB:   PRK00084
- BioCyc:   LBIF355278:LBF_0313-MONOMER
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 17599; Mature: 17599

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRVGNGIDFHKLIHEPFRPLVLAGVEIKSEFAFLGHSDADVILHAVADAILGALSLGDI
CCCCCCCCCHHHHHHCCCHHHHHCCCCHHCCEEECCCCCHHHHHHHHHHHHHHHHHHCCC
GVHFPDTDPQYKNMKSTRIIEKCLELVAEKKFKLINVDCTYVGDHPKISPIRAELNASLA
EEECCCCCCHHHCCHHHHHHHHHHHHHHHCCEEEEEEEEEEECCCCCCCHHHHHHHHHHH
NITKLPLDCVSIKATTSEGMGALGRSEGVMVMATVLIESLKK
HHHHCCCHHEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MFRVGNGIDFHKLIHEPFRPLVLAGVEIKSEFAFLGHSDADVILHAVADAILGALSLGDI
CCCCCCCCCHHHHHHCCCHHHHHCCCCHHCCEEECCCCCHHHHHHHHHHHHHHHHHHCCC
GVHFPDTDPQYKNMKSTRIIEKCLELVAEKKFKLINVDCTYVGDHPKISPIRAELNASLA
EEECCCCCCHHHCCHHHHHHHHHHHHHHHCCEEEEEEEEEEECCCCCCCHHHHHHHHHHH
NITKLPLDCVSIKATTSEGMGALGRSEGVMVMATVLIESLKK
HHHHCCCHHEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA