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The map label for this gene is ycgF [H]

Identifier: 183219705

GI number: 183219705

Start: 272513

End: 273319

Strand: Direct

Name: ycgF [H]

Synonym: LEPBI_I0281

Alternate gene names: 183219705

Gene position: 272513-273319 (Clockwise)

Preceding gene: 183219704

Following gene: 183219707

Centisome position: 7.57

GC content: 42.26

Gene sequence:

>807_bases
ATGAAACCAAGTTTTCCAGAAGCACAATTAGAAGATACACCCAACGGAAAAGTACCAAAGTTGTATAGTTGTGCGGAATG
CCGGAGCGGTGCGGGTTTAGATTTTTCCTTTTCCATGGCCTTCCAACCCATCATCGATTGGAACCAAAAAAAAATCTATT
CCCACGAAGCACTAGTTAGAGGAACAAAAGGAGAATCGGCATATTCGATTCTTTCCAAGGTCAATCAAAACAATCGTTAC
CAATTTGACCAATCTTGTCGGATCAAAGCCATCCAACTGGCAAACCAAATCCAAATCCCTGCCTTACTCAATATCAACTT
TTTACCCAATGCGGTTTACCAACCGGAGACCTGTATCCGTACAACTTTGGAAGCCAGTCGTGAGTATCAATTCCCTCTGA
ATCGATTGGTGTTTGAGCTGACAGAGGGAGAAGAAGTCCAAGACCATAACCACATCATCAATATCTTTAAAACCTATCAA
AAGTATGGGTTTTTAACTGCTATCGATGATTTTGGATCAGGGTATTCTGGGCTCAATTTACTCGCAAAGTTCCAACCCGA
TCTCATCAAACTCGATATGGAACTCATTCGGAATATTCACGCAAATTCAGTGGCTCAAAAACTGACAAAAGCGATTGCAG
GTGTTTGTCGGGAAATCGGAATCCAAGTCATTGCGGAAGGTGTGGAAACGGTAGAGGAATTGAAAGTGCTTGTGGATATG
GGAATCCATTTGTACCAAGGGTATTTGTTTTCCAAACCTGCCTTTGAATCCGCAGGTGAAGTTCACTTCCCCGAATTGAC
ATCTTGA

Upstream 100 bases:

>100_bases
TGTTGTGACCTGGTATTTAGTCGCTAAACTTCCGAAATTTTAATTTCGAAAACAAAGTTGCAAACTTTTACCTATCGTCT
AAACTGGGTAACCGAAAAAC

Downstream 100 bases:

>100_bases
TCCAATTTCCTTTTTTGAATCCAGAGTCTGATTGACTCAAATGAAAAAACTATTTAGATCCCTTCGTAAAGAATCAAATT
GATAATCACTTCGTCCTACC

Product: EAL domain-containing protein

Products: NA

Alternate protein names: Blrp [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MKPSFPEAQLEDTPNGKVPKLYSCAECRSGAGLDFSFSMAFQPIIDWNQKKIYSHEALVRGTKGESAYSILSKVNQNNRY
QFDQSCRIKAIQLANQIQIPALLNINFLPNAVYQPETCIRTTLEASREYQFPLNRLVFELTEGEEVQDHNHIINIFKTYQ
KYGFLTAIDDFGSGYSGLNLLAKFQPDLIKLDMELIRNIHANSVAQKLTKAIAGVCREIGIQVIAEGVETVEELKVLVDM
GIHLYQGYLFSKPAFESAGEVHFPELTS

Sequences:

>Translated_268_residues
MKPSFPEAQLEDTPNGKVPKLYSCAECRSGAGLDFSFSMAFQPIIDWNQKKIYSHEALVRGTKGESAYSILSKVNQNNRY
QFDQSCRIKAIQLANQIQIPALLNINFLPNAVYQPETCIRTTLEASREYQFPLNRLVFELTEGEEVQDHNHIINIFKTYQ
KYGFLTAIDDFGSGYSGLNLLAKFQPDLIKLDMELIRNIHANSVAQKLTKAIAGVCREIGIQVIAEGVETVEELKVLVDM
GIHLYQGYLFSKPAFESAGEVHFPELTS
>Mature_268_residues
MKPSFPEAQLEDTPNGKVPKLYSCAECRSGAGLDFSFSMAFQPIIDWNQKKIYSHEALVRGTKGESAYSILSKVNQNNRY
QFDQSCRIKAIQLANQIQIPALLNINFLPNAVYQPETCIRTTLEASREYQFPLNRLVFELTEGEEVQDHNHIINIFKTYQ
KYGFLTAIDDFGSGYSGLNLLAKFQPDLIKLDMELIRNIHANSVAQKLTKAIAGVCREIGIQVIAEGVETVEELKVLVDM
GIHLYQGYLFSKPAFESAGEVHFPELTS

Specific function: Binds to and releases the YcgE repressor from its bound DNA target in a blue-light-dependent (470 nm) fashion. A shift to low temperature also triggers a YcgF-medisted relief of repression by YcgE, suggesting YcgF may serve as a thermometer. Blue light ma

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1787410, Length=227, Percent_Identity=29.9559471365639, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI1787541, Length=253, Percent_Identity=31.2252964426877, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI226510982, Length=234, Percent_Identity=25.6410256410256, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI87081980, Length=129, Percent_Identity=33.3333333333333, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI87081845, Length=119, Percent_Identity=35.2941176470588, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1786507, Length=120, Percent_Identity=32.5, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1788849, Length=160, Percent_Identity=32.5, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87082096, Length=150, Percent_Identity=27.3333333333333, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1788502, Length=229, Percent_Identity=24.8908296943231, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87081921, Length=247, Percent_Identity=27.1255060728745, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1787055, Length=240, Percent_Identity=29.5833333333333, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1790496, Length=224, Percent_Identity=26.7857142857143, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87081743, Length=219, Percent_Identity=25.1141552511416, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR007024
- InterPro:   IPR001633 [H]

Pfam domain/function: PF04940 BLUF; PF00563 EAL [H]

EC number: NA

Molecular weight: Translated: 30191; Mature: 30191

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPSFPEAQLEDTPNGKVPKLYSCAECRSGAGLDFSFSMAFQPIIDWNQKKIYSHEALVR
CCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCEEHHHHHHHHHCCCHHHHHHHHHHHC
GTKGESAYSILSKVNQNNRYQFDQSCRIKAIQLANQIQIPALLNINFLPNAVYQPETCIR
CCCCHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHH
TTLEASREYQFPLNRLVFELTEGEEVQDHNHIINIFKTYQKYGFLTAIDDFGSGYSGLNL
HHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEHHCCCCCHHHHH
LAKFQPDLIKLDMELIRNIHANSVAQKLTKAIAGVCREIGIQVIAEGVETVEELKVLVDM
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIHLYQGYLFSKPAFESAGEVHFPELTS
HHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKPSFPEAQLEDTPNGKVPKLYSCAECRSGAGLDFSFSMAFQPIIDWNQKKIYSHEALVR
CCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCEEHHHHHHHHHCCCHHHHHHHHHHHC
GTKGESAYSILSKVNQNNRYQFDQSCRIKAIQLANQIQIPALLNINFLPNAVYQPETCIR
CCCCHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHH
TTLEASREYQFPLNRLVFELTEGEEVQDHNHIINIFKTYQKYGFLTAIDDFGSGYSGLNL
HHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEHHCCCCCHHHHH
LAKFQPDLIKLDMELIRNIHANSVAQKLTKAIAGVCREIGIQVIAEGVETVEELKVLVDM
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIHLYQGYLFSKPAFESAGEVHFPELTS
HHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]