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The map label for this gene is purD [H]

Identifier: 183219676

GI number: 183219676

Start: 241799

End: 243076

Strand: Direct

Name: purD [H]

Synonym: LEPBI_I0251

Alternate gene names: 183219676

Gene position: 241799-243076 (Clockwise)

Preceding gene: 183219675

Following gene: 183219677

Centisome position: 6.72

GC content: 40.45

Gene sequence:

>1278_bases
TTGGACAATCAATATAAAGTTTTATTATTAGGAAGTGGTGGCAGGGAACATGCGTTAGCTGATGCTATTTCTAAATCAAA
TGTTTTAGAATCTTTGAAAGTTTATCCAGGTAACGGGGGATTTTCAGAGGATGTTTTACTCAAAGCGGATGAAATTTCCA
TCACCGATAAATCTAAGTTCATAGAATATATCAAGTCTTCCCAAACCAATCTTGTTGTTGTGGGACCGGAAGACCCACTT
GTGAATGGAATTGCAGATTGGTGTGAAGAGATCGGGGTCCCTTGTTTTGGTCCTTCCGCCTATTGTGCGCAAGTAGAGGG
TAGTAAACATTTTGCGAAAGAAATGATGAAACGAGCAAAGGTTCCGACAGCTTCGTTTGCCGTATTTACAGACCACGAAT
CTGCTTGGAGTTTTGCTCAAAAAGAAATGTTACCACTTGTTGTCAAAGCGGATGGTTTGGCCGCTGGTAAGGGTGTCACT
GTTGCTTTTGAAATGAAAGATGTCAAACGCGCGTTAGACGAAATATTTCTCGAATCAAAATTTGGTCAAAGTGGTTATAA
GGTTGTTTTGGAGTCTTTTTTGGAAGGGGAAGAATCATCCTTATTTGTGATTACGGATGGCGAAAGGTATATGTGTTTGC
CCGCCGCCCAAGACCACAAACGCGCGTATGATGGAGATATCGGTCCAAACACTGGTGGGATGGGAGCCTATGCTCCTGCT
CCCATCGTAACTGAAGCCGTTTTAGAAAAAGTGAAATCAAGAATCATTGAGCCGATGTTAGATGATTTTAAAAGATCTGG
TCATCCTTACAAAGGACTACTTTACGTTGGTTTAATGATTACAAAAGAAGGCGAACCAAACGTAGTTGAATTTAATTGTC
GTTTTGGTGATCCTGAAACCCAGTGCGTATTACGTCTCCTCGATGAAGATATTTTGCCGATTTTTTATGCATCCGCAATA
GGGAATTTGCCTGTCAGAAATTTAAAATTAAAACAAGGTTCTTCTGCAGTTGTTGTACTTGCTGCAAAAGGGTATCCAGA
TTCTCCAGAAAAAGGAATGGTTTTGGAAATCCCACCAAACGAAGGGAATGTGGTGGTCTACCACGCAGGAACCAAAAGGG
ACAATAGTTCCCTATTGGCAAATGGTGGTCGAATTCTCGGTATCACATCTTTTGGAAAAACGCTTAAGGATGCAATAGAT
GAATGTTATACTTTTTTAAGTACAATTAAAGCTCCGAGTACATTTTATCGAAAAGATATCGGAAGGAGAGCACTCTAA

Upstream 100 bases:

>100_bases
TTTTTGGAAAAAGCAAAACCAGATGTTGTGGAAAAAGAAAGAGAAAAATACAATACTTGGAAAGAGAAATTAGAAAGTAC
AGTTAGGGCGTTGGAAAAAA

Downstream 100 bases:

>100_bases
GTGCCATTCAGTCTCTCAGGAAATTTTAAAACTTGCGAACGTTCTAAACTTCGCAATTTTGAAAAACACCTAGGGATCAT
TTTATCTGAAAACCAAACCC

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MDNQYKVLLLGSGGREHALADAISKSNVLESLKVYPGNGGFSEDVLLKADEISITDKSKFIEYIKSSQTNLVVVGPEDPL
VNGIADWCEEIGVPCFGPSAYCAQVEGSKHFAKEMMKRAKVPTASFAVFTDHESAWSFAQKEMLPLVVKADGLAAGKGVT
VAFEMKDVKRALDEIFLESKFGQSGYKVVLESFLEGEESSLFVITDGERYMCLPAAQDHKRAYDGDIGPNTGGMGAYAPA
PIVTEAVLEKVKSRIIEPMLDDFKRSGHPYKGLLYVGLMITKEGEPNVVEFNCRFGDPETQCVLRLLDEDILPIFYASAI
GNLPVRNLKLKQGSSAVVVLAAKGYPDSPEKGMVLEIPPNEGNVVVYHAGTKRDNSSLLANGGRILGITSFGKTLKDAID
ECYTFLSTIKAPSTFYRKDIGRRAL

Sequences:

>Translated_425_residues
MDNQYKVLLLGSGGREHALADAISKSNVLESLKVYPGNGGFSEDVLLKADEISITDKSKFIEYIKSSQTNLVVVGPEDPL
VNGIADWCEEIGVPCFGPSAYCAQVEGSKHFAKEMMKRAKVPTASFAVFTDHESAWSFAQKEMLPLVVKADGLAAGKGVT
VAFEMKDVKRALDEIFLESKFGQSGYKVVLESFLEGEESSLFVITDGERYMCLPAAQDHKRAYDGDIGPNTGGMGAYAPA
PIVTEAVLEKVKSRIIEPMLDDFKRSGHPYKGLLYVGLMITKEGEPNVVEFNCRFGDPETQCVLRLLDEDILPIFYASAI
GNLPVRNLKLKQGSSAVVVLAAKGYPDSPEKGMVLEIPPNEGNVVVYHAGTKRDNSSLLANGGRILGITSFGKTLKDAID
ECYTFLSTIKAPSTFYRKDIGRRAL
>Mature_425_residues
MDNQYKVLLLGSGGREHALADAISKSNVLESLKVYPGNGGFSEDVLLKADEISITDKSKFIEYIKSSQTNLVVVGPEDPL
VNGIADWCEEIGVPCFGPSAYCAQVEGSKHFAKEMMKRAKVPTASFAVFTDHESAWSFAQKEMLPLVVKADGLAAGKGVT
VAFEMKDVKRALDEIFLESKFGQSGYKVVLESFLEGEESSLFVITDGERYMCLPAAQDHKRAYDGDIGPNTGGMGAYAPA
PIVTEAVLEKVKSRIIEPMLDDFKRSGHPYKGLLYVGLMITKEGEPNVVEFNCRFGDPETQCVLRLLDEDILPIFYASAI
GNLPVRNLKLKQGSSAVVVLAAKGYPDSPEKGMVLEIPPNEGNVVVYHAGTKRDNSSLLANGGRILGITSFGKTLKDAID
ECYTFLSTIKAPSTFYRKDIGRRAL

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=427, Percent_Identity=43.559718969555, Blast_Score=341, Evalue=7e-94,
Organism=Homo sapiens, GI209869995, Length=427, Percent_Identity=43.559718969555, Blast_Score=341, Evalue=7e-94,
Organism=Homo sapiens, GI209869993, Length=427, Percent_Identity=43.559718969555, Blast_Score=341, Evalue=7e-94,
Organism=Homo sapiens, GI28416899, Length=427, Percent_Identity=43.559718969555, Blast_Score=341, Evalue=9e-94,
Organism=Escherichia coli, GI1790438, Length=423, Percent_Identity=42.3167848699764, Blast_Score=355, Evalue=5e-99,
Organism=Caenorhabditis elegans, GI17567511, Length=423, Percent_Identity=39.2434988179669, Blast_Score=265, Evalue=3e-71,
Organism=Saccharomyces cerevisiae, GI6321203, Length=441, Percent_Identity=42.4036281179138, Blast_Score=335, Evalue=6e-93,
Organism=Drosophila melanogaster, GI62471619, Length=431, Percent_Identity=41.2993039443155, Blast_Score=297, Evalue=7e-81,
Organism=Drosophila melanogaster, GI24582400, Length=431, Percent_Identity=41.2993039443155, Blast_Score=297, Evalue=1e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 46260; Mature: 46260

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNQYKVLLLGSGGREHALADAISKSNVLESLKVYPGNGGFSEDVLLKADEISITDKSKF
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCCCEEEEECCEEECCHHHH
IEYIKSSQTNLVVVGPEDPLVNGIADWCEEIGVPCFGPSAYCAQVEGSKHFAKEMMKRAK
HHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCHHEEECCCCHHHHHHHHHHHC
VPTASFAVFTDHESAWSFAQKEMLPLVVKADGLAAGKGVTVAFEMKDVKRALDEIFLESK
CCCCEEEEEECCHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHHHH
FGQSGYKVVLESFLEGEESSLFVITDGERYMCLPAAQDHKRAYDGDIGPNTGGMGAYAPA
CCCCHHHHHHHHHHCCCCCEEEEEECCCEEEEECCCCCHHHCCCCCCCCCCCCCCCCCCC
PIVTEAVLEKVKSRIIEPMLDDFKRSGHPYKGLLYVGLMITKEGEPNVVEFNCRFGDPET
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCEEEEEEECCCCHH
QCVLRLLDEDILPIFYASAIGNLPVRNLKLKQGSSAVVVLAAKGYPDSPEKGMVLEIPPN
HHHHHHHHCCHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCEEEEECCC
EGNVVVYHAGTKRDNSSLLANGGRILGITSFGKTLKDAIDECYTFLSTIKAPSTFYRKDI
CCCEEEEECCCCCCCCCEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
GRRAL
CCCCC
>Mature Secondary Structure
MDNQYKVLLLGSGGREHALADAISKSNVLESLKVYPGNGGFSEDVLLKADEISITDKSKF
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCCCEEEEECCEEECCHHHH
IEYIKSSQTNLVVVGPEDPLVNGIADWCEEIGVPCFGPSAYCAQVEGSKHFAKEMMKRAK
HHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCHHEEECCCCHHHHHHHHHHHC
VPTASFAVFTDHESAWSFAQKEMLPLVVKADGLAAGKGVTVAFEMKDVKRALDEIFLESK
CCCCEEEEEECCHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHHHH
FGQSGYKVVLESFLEGEESSLFVITDGERYMCLPAAQDHKRAYDGDIGPNTGGMGAYAPA
CCCCHHHHHHHHHHCCCCCEEEEEECCCEEEEECCCCCHHHCCCCCCCCCCCCCCCCCCC
PIVTEAVLEKVKSRIIEPMLDDFKRSGHPYKGLLYVGLMITKEGEPNVVEFNCRFGDPET
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCEEEEEEECCCCHH
QCVLRLLDEDILPIFYASAIGNLPVRNLKLKQGSSAVVVLAAKGYPDSPEKGMVLEIPPN
HHHHHHHHCCHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCEEEEECCC
EGNVVVYHAGTKRDNSSLLANGGRILGITSFGKTLKDAIDECYTFLSTIKAPSTFYRKDI
CCCEEEEECCCCCCCCCEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
GRRAL
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA