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The map label for this gene is ilvE [H]

Identifier: 182680445

GI number: 182680445

Start: 4025804

End: 4026691

Strand: Reverse

Name: ilvE [H]

Synonym: Bind_3545

Alternate gene names: 182680445

Gene position: 4026691-4025804 (Counterclockwise)

Preceding gene: 182680448

Following gene: 182680443

Centisome position: 96.56

GC content: 57.66

Gene sequence:

>888_bases
ATGTCAGCATTGCCATTCGACCAACGTGATGGATACATCTGGATGAATGGTCGGATCGTAGCCTGGGCCGATGCCAAGCT
GCATGTTCTGTCGCATGGTTTGCATTATGCCTCCTGCGTCTTCGAGGGCGAGCGGGCTTATGAAGGCCGTATTTTCAAAT
CGCGCGAACATTCCGAACGGTTCCGCGCCTCCGCTCAAGTCCTTGATTTCGAACTGCCCTATTCGGTGGCAGAACTCGAC
GCGGCGAAACAACTCGTCGTTGACAAGAACAATTTCCAGAATTGCTATGTGCGGCCGGTCGCCTGGCGTGGCAGTGAAAT
GATGGCGGTAGCTGCCCAGAATTCGACCATCAATGTCGCCATTGCCGCCTGGGATTGGCCGAGCATGTTCGACATGGAAA
CCAAAATGAAGGGCATCCGGCTCGATATCGCTGATTATCGCCGCCCTGATCCGCAGACCGCGCCCTGCCATGCGAAGGCA
GCCGGTCTCTACATGATTTGTACAATCTCAAAACATCGGGCCGAACGGCGCGGCTATGCCGATGCGATGATGCTCGATTG
GCAGGGCCGCGTCGCGGAATGCACGGGCGCCAATCTGTTCTTCACCCAGAAGGGCGCTCTGCACACGCCGATCGCGGATT
GTTTCCTCAACGGCATCACGCGCCAGACGGTCATCGAACTCGCCAAGAGGCGTGGTCTCGAAGTGGTGGAACGGCGTATC
ATGCCGGATGAACTGTCCGGTTTCGACGAATGTTTTGTCTGCGGCACCGGTGCCGAAGTGACACCAGTTTCCGAGATCGG
TCCCTATCGTTTCACGCCTGGTGTGATTTCGCGCGCCCTGATCGAGGATTACAGCCTGGAAGTCCGGCCGCGCCAGCAGG
CCGCCTGA

Upstream 100 bases:

>100_bases
TGTCGAGCAAGGATTTCGGAGGCGATCGTCGGCCAGGCATGCCACGCCCAATTGCCGCGTGGCTTTCCCATCTCTAGTCA
TAAAGAGAGGATTTCCCTCG

Downstream 100 bases:

>100_bases
GACCTTCTTGTAAGGGTCTCTCAGTTAATCGATTGGCGATGGCGGACCCGCCGTCGCCGATGCGTGACCTGCACCGATGC
AATCCGCGCTTATGAAATGG

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MSALPFDQRDGYIWMNGRIVAWADAKLHVLSHGLHYASCVFEGERAYEGRIFKSREHSERFRASAQVLDFELPYSVAELD
AAKQLVVDKNNFQNCYVRPVAWRGSEMMAVAAQNSTINVAIAAWDWPSMFDMETKMKGIRLDIADYRRPDPQTAPCHAKA
AGLYMICTISKHRAERRGYADAMMLDWQGRVAECTGANLFFTQKGALHTPIADCFLNGITRQTVIELAKRRGLEVVERRI
MPDELSGFDECFVCGTGAEVTPVSEIGPYRFTPGVISRALIEDYSLEVRPRQQAA

Sequences:

>Translated_295_residues
MSALPFDQRDGYIWMNGRIVAWADAKLHVLSHGLHYASCVFEGERAYEGRIFKSREHSERFRASAQVLDFELPYSVAELD
AAKQLVVDKNNFQNCYVRPVAWRGSEMMAVAAQNSTINVAIAAWDWPSMFDMETKMKGIRLDIADYRRPDPQTAPCHAKA
AGLYMICTISKHRAERRGYADAMMLDWQGRVAECTGANLFFTQKGALHTPIADCFLNGITRQTVIELAKRRGLEVVERRI
MPDELSGFDECFVCGTGAEVTPVSEIGPYRFTPGVISRALIEDYSLEVRPRQQAA
>Mature_294_residues
SALPFDQRDGYIWMNGRIVAWADAKLHVLSHGLHYASCVFEGERAYEGRIFKSREHSERFRASAQVLDFELPYSVAELDA
AKQLVVDKNNFQNCYVRPVAWRGSEMMAVAAQNSTINVAIAAWDWPSMFDMETKMKGIRLDIADYRRPDPQTAPCHAKAA
GLYMICTISKHRAERRGYADAMMLDWQGRVAECTGANLFFTQKGALHTPIADCFLNGITRQTVIELAKRRGLEVVERRIM
PDELSGFDECFVCGTGAEVTPVSEIGPYRFTPGVISRALIEDYSLEVRPRQQAA

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=261, Percent_Identity=39.0804597701149, Blast_Score=186, Evalue=1e-48,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 33253; Mature: 33122

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALPFDQRDGYIWMNGRIVAWADAKLHVLSHGLHYASCVFEGERAYEGRIFKSREHSER
CCCCCCCCCCCEEEECCEEEEEECCEEEEHHCCCEEEEEEECCCCCCCCEEECCCHHHHH
FRASAQVLDFELPYSVAELDAAKQLVVDKNNFQNCYVRPVAWRGSEMMAVAAQNSTINVA
HHHHHHEEEEECCCHHHHHHHHHHEEECCCCCCCEEEEEEEECCCEEEEEEECCCEEEEE
IAAWDWPSMFDMETKMKGIRLDIADYRRPDPQTAPCHAKAAGLYMICTISKHRAERRGYA
EEEECCCCCCCHHHHCCCEEEEHHHCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHCCCC
DAMMLDWQGRVAECTGANLFFTQKGALHTPIADCFLNGITRQTVIELAKRRGLEVVERRI
CEEEEECCCCEEEECCCEEEEECCCCCCCCHHHHHHHCCCHHHHHHHHHHCCHHHHHHHC
MPDELSGFDECFVCGTGAEVTPVSEIGPYRFTPGVISRALIEDYSLEVRPRQQAA
CCHHHCCCCEEEEECCCCCCCCHHHCCCCCCCHHHHHHHHHHHCCEEECCHHCCC
>Mature Secondary Structure 
SALPFDQRDGYIWMNGRIVAWADAKLHVLSHGLHYASCVFEGERAYEGRIFKSREHSER
CCCCCCCCCCEEEECCEEEEEECCEEEEHHCCCEEEEEEECCCCCCCCEEECCCHHHHH
FRASAQVLDFELPYSVAELDAAKQLVVDKNNFQNCYVRPVAWRGSEMMAVAAQNSTINVA
HHHHHHEEEEECCCHHHHHHHHHHEEECCCCCCCEEEEEEEECCCEEEEEEECCCEEEEE
IAAWDWPSMFDMETKMKGIRLDIADYRRPDPQTAPCHAKAAGLYMICTISKHRAERRGYA
EEEECCCCCCCHHHHCCCEEEEHHHCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHCCCC
DAMMLDWQGRVAECTGANLFFTQKGALHTPIADCFLNGITRQTVIELAKRRGLEVVERRI
CEEEEECCCCEEEECCCEEEEECCCCCCCCHHHHHHHCCCHHHHHHHHHHCCHHHHHHHC
MPDELSGFDECFVCGTGAEVTPVSEIGPYRFTPGVISRALIEDYSLEVRPRQQAA
CCHHHCCCCEEEEECCCCCCCCHHHCCCCCCCHHHHHHHHHHHCCEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA