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The map label for this gene is nodE [H]

Identifier: 182680387

GI number: 182680387

Start: 3962546

End: 3963802

Strand: Reverse

Name: nodE [H]

Synonym: Bind_3487

Alternate gene names: 182680387

Gene position: 3963802-3962546 (Counterclockwise)

Preceding gene: 182680388

Following gene: 182680386

Centisome position: 95.05

GC content: 61.02

Gene sequence:

>1257_bases
ATGAATGATCGGGGCGGAATGTTTGATTCGGGACTGAACGGCGCGGGAAATAAGGGCCAGAGGCGCGTGGTCATCACCGG
TATGGGAGCGGTCTCGGCTGCTGGTGTCGGTGCTCAGGCTTTGTGGCAAGCGGCTCGCGATGGGCGCTCCTGCATCGCCG
AAACCCAATTCGTCCGCCCCTATCGCGGCCGTATTCGGCGGACCGCGCAAGTGCATGATTTCGTGCCGGAAGATCATCTC
GATTCCAATGTCCTGCCGATGTGTGACCCCGTCACACAATATCTCCTGGTGGCGGCCGACGAGGCCATGGCGCAGGCGGG
CCTTTCACGCTCAGAGCGGCTCGGATCAAGGGCGGCGGCCATTATCGGCACGGGCGTTGGCGGCATGCATACGATCGAGA
ATGGCCTCCATCTCGCTCTGATCGAACAGGGTCGTCCCTATCCCCTGACTGTTCCCAAACTTATTCCGAGCTCCTGCCCC
TCGCATATCAGCATTCGCTACGGCGTAATGGGTCCGAGTTTCGCGGTCGCGAGCGCTTGTTCCTCAGCCACCCAGGCGAT
TGGGATCGGCGCGCATATGATCCGTGCCGGTCTGGTCGATCGCGCCATTGTCGGTGGCACGGAGGATTGCCTCACCAATA
GTTCCATGCTCGCCTGGGAAGCCTTGCGTGTGCTGACGCCCGATTTCTGCCGGCCTTTCTCGAAAGGGCGCAACGGCATG
GTGCTCGGCGCCGGTGCGGGGGTTTTCATTCTTGAGGCCGAGGAGGTTGCTCTCGCCCGTGGTGCCCAGCCCCTGGCGGA
ACTTGCCGGCTATGGCACGTCGACCGATGCCAAGGATCACGTCCGTCCAGATCCGCAAGGCTGCGCTACCTCGATGACCA
ATGCCTTGCTGGATGCGGGCCTTGCACCGGATGATATCGATTACATCAATGCGCATGGCACCGGCACGGTCATCAACGAT
ACCAATGAATCGGAAGCTGTCGGCATCGTGTTCGGTGATCGGAAACCGCCGATTTCTTCCAGCAAGCCGATCCATGGCCA
TGGGCTCGGCGCGGGTGGTGCCCTTGAATTGGTGGTAACGATCAATGCCGTGCGGGAGAATATCGCGCCGCCGACGATCA
ATTTCAGCGAGGCCGATGCGAAATGCCCGGTCGATGCAATCCCCAACAAGGCGCGCGCAATGCCGATCCGTGTCGCTCTC
TCCAATTCCTTCGCCTTTGGCGGCATCAATGCGACGCTTGTGGTGCGGCAGGTCTAA

Upstream 100 bases:

>100_bases
TCTATATTCCCATGGACGCGCCTTTCCACGAAGCCACTGACGTCAAAAGTCTCATTGACGCGATTGCCGCCTATATCATC
AAGGAAAAGACCAAAACAGA

Downstream 100 bases:

>100_bases
GCAGCCTTGCGTGGCCAAAGCCACGATTGATTTTGCTTTTTTAAGCAAAATCAATGCTGCCAAGAATTTTAAGTATAAGC
AAACTTTGGAAAGTTTGCTT

Product: Beta-ketoacyl synthase

Products: [acyl carrier protein]; acetoacetyl-ACP; CO2; [acyl carrier protein]; beta-ketoacyl-ACP [C]

Alternate protein names: Host-specificity of nodulation protein B [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MNDRGGMFDSGLNGAGNKGQRRVVITGMGAVSAAGVGAQALWQAARDGRSCIAETQFVRPYRGRIRRTAQVHDFVPEDHL
DSNVLPMCDPVTQYLLVAADEAMAQAGLSRSERLGSRAAAIIGTGVGGMHTIENGLHLALIEQGRPYPLTVPKLIPSSCP
SHISIRYGVMGPSFAVASACSSATQAIGIGAHMIRAGLVDRAIVGGTEDCLTNSSMLAWEALRVLTPDFCRPFSKGRNGM
VLGAGAGVFILEAEEVALARGAQPLAELAGYGTSTDAKDHVRPDPQGCATSMTNALLDAGLAPDDIDYINAHGTGTVIND
TNESEAVGIVFGDRKPPISSSKPIHGHGLGAGGALELVVTINAVRENIAPPTINFSEADAKCPVDAIPNKARAMPIRVAL
SNSFAFGGINATLVVRQV

Sequences:

>Translated_418_residues
MNDRGGMFDSGLNGAGNKGQRRVVITGMGAVSAAGVGAQALWQAARDGRSCIAETQFVRPYRGRIRRTAQVHDFVPEDHL
DSNVLPMCDPVTQYLLVAADEAMAQAGLSRSERLGSRAAAIIGTGVGGMHTIENGLHLALIEQGRPYPLTVPKLIPSSCP
SHISIRYGVMGPSFAVASACSSATQAIGIGAHMIRAGLVDRAIVGGTEDCLTNSSMLAWEALRVLTPDFCRPFSKGRNGM
VLGAGAGVFILEAEEVALARGAQPLAELAGYGTSTDAKDHVRPDPQGCATSMTNALLDAGLAPDDIDYINAHGTGTVIND
TNESEAVGIVFGDRKPPISSSKPIHGHGLGAGGALELVVTINAVRENIAPPTINFSEADAKCPVDAIPNKARAMPIRVAL
SNSFAFGGINATLVVRQV
>Mature_418_residues
MNDRGGMFDSGLNGAGNKGQRRVVITGMGAVSAAGVGAQALWQAARDGRSCIAETQFVRPYRGRIRRTAQVHDFVPEDHL
DSNVLPMCDPVTQYLLVAADEAMAQAGLSRSERLGSRAAAIIGTGVGGMHTIENGLHLALIEQGRPYPLTVPKLIPSSCP
SHISIRYGVMGPSFAVASACSSATQAIGIGAHMIRAGLVDRAIVGGTEDCLTNSSMLAWEALRVLTPDFCRPFSKGRNGM
VLGAGAGVFILEAEEVALARGAQPLAELAGYGTSTDAKDHVRPDPQGCATSMTNALLDAGLAPDDIDYINAHGTGTVIND
TNESEAVGIVFGDRKPPISSSKPIHGHGLGAGGALELVVTINAVRENIAPPTINFSEADAKCPVDAIPNKARAMPIRVAL
SNSFAFGGINATLVVRQV

Specific function: Proposed to synthesize NOD factor fatty acyl chain. Involved in the synthesis of a highly unsaturated fatty acid moiety, which forms part of a lipo-oligosaccharide that is responsible for host specificity [H]

COG id: COG0304

COG function: function code IQ; 3-oxoacyl-(acyl-carrier-protein) synthase

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-ketoacyl-ACP synthases family [H]

Homologues:

Organism=Homo sapiens, GI8923559, Length=421, Percent_Identity=33.9667458432304, Blast_Score=198, Evalue=8e-51,
Organism=Homo sapiens, GI223972627, Length=171, Percent_Identity=35.672514619883, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI41872631, Length=422, Percent_Identity=26.5402843601896, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1787337, Length=413, Percent_Identity=36.0774818401937, Blast_Score=215, Evalue=4e-57,
Organism=Escherichia coli, GI1788663, Length=414, Percent_Identity=30.4347826086957, Blast_Score=174, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI71983541, Length=411, Percent_Identity=31.8734793187348, Blast_Score=188, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI71983535, Length=254, Percent_Identity=36.2204724409449, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI212642053, Length=348, Percent_Identity=27.0114942528736, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17550940, Length=269, Percent_Identity=26.7657992565056, Blast_Score=89, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6320904, Length=449, Percent_Identity=30.0668151447661, Blast_Score=163, Evalue=5e-41,
Organism=Drosophila melanogaster, GI24644341, Length=419, Percent_Identity=36.2768496420048, Blast_Score=224, Evalue=6e-59,
Organism=Drosophila melanogaster, GI19920632, Length=343, Percent_Identity=30.0291545189504, Blast_Score=101, Evalue=9e-22,
Organism=Drosophila melanogaster, GI221330659, Length=343, Percent_Identity=30.0291545189504, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24581345, Length=355, Percent_Identity=28.169014084507, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000794
- InterPro:   IPR018201
- InterPro:   IPR014031
- InterPro:   IPR014030
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615 [H]

Pfam domain/function: PF00109 ketoacyl-synt; PF02801 Ketoacyl-synt_C [H]

EC number: 2.3.1.41 [C]

Molecular weight: Translated: 43466; Mature: 43466

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00606 B_KETOACYL_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDRGGMFDSGLNGAGNKGQRRVVITGMGAVSAAGVGAQALWQAARDGRSCIAETQFVRP
CCCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHCCCCHHHHHHHHHCCHHHHHHHHHCCH
YRGRIRRTAQVHDFVPEDHLDSNVLPMCDPVTQYLLVAADEAMAQAGLSRSERLGSRAAA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHEE
IIGTGVGGMHTIENGLHLALIEQGRPYPLTVPKLIPSSCPSHISIRYGVMGPSFAVASAC
EEECCCCCHHHHCCCCEEEEECCCCCCCEEHHHHCCCCCCCCEEEEEECCCCHHHHHHHH
SSATQAIGIGAHMIRAGLVDRAIVGGTEDCLTNSSMLAWEALRVLTPDFCRPFSKGRNGM
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHCCHHHHHHHHCCCCCE
VLGAGAGVFILEAEEVALARGAQPLAELAGYGTSTDAKDHVRPDPQGCATSMTNALLDAG
EEECCCCEEEEEHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
LAPDDIDYINAHGTGTVINDTNESEAVGIVFGDRKPPISSSKPIHGHGLGAGGALELVVT
CCCCCCCEEECCCCEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEE
INAVRENIAPPTINFSEADAKCPVDAIPNKARAMPIRVALSNSFAFGGINATLVVRQV
EEHHHHCCCCCCCCCCCCCCCCCHHHCCCCCCCCEEEEEECCCEEECCCCEEEEEEEC
>Mature Secondary Structure
MNDRGGMFDSGLNGAGNKGQRRVVITGMGAVSAAGVGAQALWQAARDGRSCIAETQFVRP
CCCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHCCCCHHHHHHHHHCCHHHHHHHHHCCH
YRGRIRRTAQVHDFVPEDHLDSNVLPMCDPVTQYLLVAADEAMAQAGLSRSERLGSRAAA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHEE
IIGTGVGGMHTIENGLHLALIEQGRPYPLTVPKLIPSSCPSHISIRYGVMGPSFAVASAC
EEECCCCCHHHHCCCCEEEEECCCCCCCEEHHHHCCCCCCCCEEEEEECCCCHHHHHHHH
SSATQAIGIGAHMIRAGLVDRAIVGGTEDCLTNSSMLAWEALRVLTPDFCRPFSKGRNGM
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHCCHHHHHHHHCCCCCE
VLGAGAGVFILEAEEVALARGAQPLAELAGYGTSTDAKDHVRPDPQGCATSMTNALLDAG
EEECCCCEEEEEHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
LAPDDIDYINAHGTGTVINDTNESEAVGIVFGDRKPPISSSKPIHGHGLGAGGALELVVT
CCCCCCCEEECCCCEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEEE
INAVRENIAPPTINFSEADAKCPVDAIPNKARAMPIRVALSNSFAFGGINATLVVRQV
EEHHHHCCCCCCCCCCCCCCCCCHHHCCCCCCCCEEEEEECCCEEECCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: None [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.04 {acetyl-ACP}} 0.031 {acetyl-ACP}} 0.138 {cis-9-hexadecenoyl-ACP}} 0.017 {cis-9-hexadecenoyl-ACP}} 0.012 {cis-3-decenoyl-ACP}} [C]

Substrates: acetyl-ACP; malonyl-ACP; acyl-ACP [C]

Specific reaction: acetyl-ACP + malonyl-ACP = [acyl carrier protein] + acetoacetyl-ACP + CO2 acyl-ACP + malonyl-ACP = [acyl carrier protein] + beta-ketoacyl-ACP + CO2 [C]

General reaction: Acyl group transfer [C]

Inhibitor: Acetyl-CoA; Acyl-CoA; Iodoacetamide; N-Ethyl maleimide acyl -ACP-derivatives protect, malonyl-ACP-decarboxylation, Cerulenin [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9100384 [H]