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The map label for this gene is glpF [C]

Identifier: 182680385

GI number: 182680385

Start: 3960918

End: 3961808

Strand: Reverse

Name: glpF [C]

Synonym: Bind_3485

Alternate gene names: 182680385

Gene position: 3961808-3960918 (Counterclockwise)

Preceding gene: 182680386

Following gene: 182680382

Centisome position: 95.0

GC content: 59.6

Gene sequence:

>891_bases
TTGCGCGCGCCTCATCTGCATCTCCCGCATTTTCATCTTCCTTTGCATTTTCGGGATCGGCCACTTGCGGGCAAGCCTGA
TCCTTCCGTGCCATTCCACTGGAAGCTCTACATCTGCGAGAGCCTGGCAACGGCGATCTTGATGATCTGCGGCATTATCT
CCGTCACGCTCCTGACCGCGCCCGGCAGTCCTGTTGCTGAGCTGCTTGGGGCTCATTCTACTGTGCAAATCGCGCTATGC
GGCCTGTGTTTCGGCCTTTCGGGGTCGATTGCAGCCTATACGCCCTTCGGCAAGGTCAGTGGCGCCCATCTCAATCCGTC
CGTCACCCTGGCCTTTCTGCTCGCTGGCAGGATCGTCTGGCTCGATGCATTCGGCTATTTCTCCGCCCAGATCATCGGTG
CGATTGAGGGGACCTTGCTGGTGTCCCTGTTCGGCGACGCCGTCGAGCCATGGGGGCAATGGGCTTATGCCGCCCATTAC
GCGGCGACCATTCCCAATCCTCAGGTCAGCGCCTTTATTCCGCTGCTCTCCGAAACCGGTGTGACGGCGCTTTTGATCGG
CATGCTCTACTGGCTCGCCTCGCATCAGGAAATGCGCTGGCTGACGCCCTGGGCGGGTGGCCTTTTCTTTCTGGTGATGA
ACCCGCTGACCGCCTGGCTGTCCGGCAATAGTGTCAATCTGGCAAGGAGTTTCGCGCCCGCCGTGTTTTCAGCGGAATGG
AGCGGCTTCTGGATTTATATCCTCGGCCCCTTCGCCGGTTCGGCCCTGGCCGTTTATTGCATCAAGACCGATCTGTTCGG
CAAGATTCATCTTGCCGAGGCGCGGCTTATCAATTTCGGCCACAAAGGCCGCGTGCCGGATCTGACCAATCCCGAGGCCA
AGAGCCCCTGA

Upstream 100 bases:

>100_bases
AATCATGGCATCATGGTTTCAATCTTTGAAAAAACTCGACCCGTAAAATTCGGCCGGGAGGTGGCGTCCATTTGAGTTCC
CATGCTTTGGGGAATTAGAA

Downstream 100 bases:

>100_bases
CTCCGCCGAAAGGGAACACTCAAAATTCCAGCGTCTTTAGGAACAGTCTTTCTGGACGCGTTCCAGCGCTTGGCTGAGGC
CGCCGAGTGAATAAATATCC

Product: major intrinsic protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: Aquaporin Z; MIP Family Channel Protein; Glycerol Uptake Facilitator Related Permease; Pore-Forming Membrane Protein SmpX-Like Protein; Glycerol Uptake Facilitator Protein; Aquaporine Z; MIP Family Channel Proteins; MIP Family Transporter; Aquaporin

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MRAPHLHLPHFHLPLHFRDRPLAGKPDPSVPFHWKLYICESLATAILMICGIISVTLLTAPGSPVAELLGAHSTVQIALC
GLCFGLSGSIAAYTPFGKVSGAHLNPSVTLAFLLAGRIVWLDAFGYFSAQIIGAIEGTLLVSLFGDAVEPWGQWAYAAHY
AATIPNPQVSAFIPLLSETGVTALLIGMLYWLASHQEMRWLTPWAGGLFFLVMNPLTAWLSGNSVNLARSFAPAVFSAEW
SGFWIYILGPFAGSALAVYCIKTDLFGKIHLAEARLINFGHKGRVPDLTNPEAKSP

Sequences:

>Translated_296_residues
MRAPHLHLPHFHLPLHFRDRPLAGKPDPSVPFHWKLYICESLATAILMICGIISVTLLTAPGSPVAELLGAHSTVQIALC
GLCFGLSGSIAAYTPFGKVSGAHLNPSVTLAFLLAGRIVWLDAFGYFSAQIIGAIEGTLLVSLFGDAVEPWGQWAYAAHY
AATIPNPQVSAFIPLLSETGVTALLIGMLYWLASHQEMRWLTPWAGGLFFLVMNPLTAWLSGNSVNLARSFAPAVFSAEW
SGFWIYILGPFAGSALAVYCIKTDLFGKIHLAEARLINFGHKGRVPDLTNPEAKSP
>Mature_296_residues
MRAPHLHLPHFHLPLHFRDRPLAGKPDPSVPFHWKLYICESLATAILMICGIISVTLLTAPGSPVAELLGAHSTVQIALC
GLCFGLSGSIAAYTPFGKVSGAHLNPSVTLAFLLAGRIVWLDAFGYFSAQIIGAIEGTLLVSLFGDAVEPWGQWAYAAHY
AATIPNPQVSAFIPLLSETGVTALLIGMLYWLASHQEMRWLTPWAGGLFFLVMNPLTAWLSGNSVNLARSFAPAVFSAEW
SGFWIYILGPFAGSALAVYCIKTDLFGKIHLAEARLINFGHKGRVPDLTNPEAKSP

Specific function: Transporter Of Glycerol Across The Cytoplasmic Membrane, With Limited Permeability To Water And Small Uncharged Compounds Such As Polyols. [C]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI6912506, Length=264, Percent_Identity=28.030303030303, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI4502181, Length=190, Percent_Identity=34.2105263157895, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI4755125, Length=190, Percent_Identity=34.2105263157895, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI157266307, Length=248, Percent_Identity=28.6290322580645, Blast_Score=65, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17531431, Length=239, Percent_Identity=28.8702928870293, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17531429, Length=239, Percent_Identity=28.8702928870293, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17558372, Length=176, Percent_Identity=31.8181818181818, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24652747, Length=226, Percent_Identity=30.5309734513274, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI45551084, Length=226, Percent_Identity=30.5309734513274, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24762342, Length=184, Percent_Identity=33.695652173913, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24762344, Length=185, Percent_Identity=31.8918918918919, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24762346, Length=185, Percent_Identity=31.8918918918919, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24762348, Length=185, Percent_Identity=31.8918918918919, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI20130305, Length=185, Percent_Identity=31.8918918918919, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 31961; Mature: 31961

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAPHLHLPHFHLPLHFRDRPLAGKPDPSVPFHWKLYICESLATAILMICGIISVTLLTA
CCCCCCCCCEEEECEEECCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHEEEC
PGSPVAELLGAHSTVQIALCGLCFGLSGSIAAYTPFGKVSGAHLNPSVTLAFLLAGRIVW
CCCHHHHHHCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCEEE
LDAFGYFSAQIIGAIEGTLLVSLFGDAVEPWGQWAYAAHYAATIPNPQVSAFIPLLSETG
EEHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHEEEECCCCCHHHHHHHHHCCC
VTALLIGMLYWLASHQEMRWLTPWAGGLFFLVMNPLTAWLSGNSVNLARSFAPAVFSAEW
HHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCEEHHHHHCCHHEECCC
SGFWIYILGPFAGSALAVYCIKTDLFGKIHLAEARLINFGHKGRVPDLTNPEAKSP
CCEEEEEECCCCCCEEEEEEEEECCCCEEEEEHHHEEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRAPHLHLPHFHLPLHFRDRPLAGKPDPSVPFHWKLYICESLATAILMICGIISVTLLTA
CCCCCCCCCEEEECEEECCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHEEEC
PGSPVAELLGAHSTVQIALCGLCFGLSGSIAAYTPFGKVSGAHLNPSVTLAFLLAGRIVW
CCCHHHHHHCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCEEE
LDAFGYFSAQIIGAIEGTLLVSLFGDAVEPWGQWAYAAHYAATIPNPQVSAFIPLLSETG
EEHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHEEEECCCCCHHHHHHHHHCCC
VTALLIGMLYWLASHQEMRWLTPWAGGLFFLVMNPLTAWLSGNSVNLARSFAPAVFSAEW
HHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCEEHHHHHCCHHEECCC
SGFWIYILGPFAGSALAVYCIKTDLFGKIHLAEARLINFGHKGRVPDLTNPEAKSP
CCEEEEEECCCCCCEEEEEEEEECCCCEEEEEHHHEEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA