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The map label for this gene is yafC [H]

Identifier: 182680170

GI number: 182680170

Start: 3716250

End: 3717158

Strand: Reverse

Name: yafC [H]

Synonym: Bind_3267

Alternate gene names: 182680170

Gene position: 3717158-3716250 (Counterclockwise)

Preceding gene: 182680179

Following gene: 182680169

Centisome position: 89.14

GC content: 57.21

Gene sequence:

>909_bases
ATGCCACTCGTCGAAGATCTTCATTGTTTCGTCCGTGTCGCGGAATGCGGCTCCTTTTCGATCGTCGCCCGGGAAAATAT
GGTGAGCCAGGCAACGATCGGGCGTCGGATTGGGACGCTCGAGGATCATTTCAAGATCCTCCTGTTCCATCGCTCGACGC
GCAAAATCGTCCTGACCGATGCCGGCCAGAGCTTTCTCGTTTATGCCCGGCGCATTCTGCAGGAGTTCGAGGCCGCGGAA
AACGCTCTTGGCCGCCATAGCGCCGAGCCAAAGGGTCGCGTCCGTGTCGCCTCGACCAATGCCTTCGGCCAATATCTGAT
CCGAGGCCTTGCGCAATTCAACGAACGCTATCCGGGTCTCCATATCGACCTTGTGCTCGGCGATGGATTCATCAATCTGA
TCGAGGACGGTTTCGATCTTGCCTTAAAGATCGGCGAGGTCACTGAATCCTCGGTCGTCGCCCGCCATCTCGGCGATGTG
CCGCGTTCACTCGTGGCATCACCCGCCTATCTCGCCAAACACGGCGCGCCGGAAGATGCTTCCGCATTGCAGCATCATGA
ATGCGTTCTGTTCAGTTATGGCGCGACACGGCAGGTTTGGAATGTCGGCGGCAGTCTCGTGCGTGTATCCGGCTCCTTTC
GGACCAATAGCAGCATTGCGCAGTTCGAGGCGGTGCGCAATGGCATGGGGATCAGCCTGTTTCCCTGGTTCCAGGTCGAT
GAGGATGTCAGAAGTGGACGTCTTATCCGCCTGTTGCCGGATATGCCGATCGAGCCCATTCATTTTTACATGACTTATCC
CGCCTATCGGACCTTGCCGCCGCGCACACGCGTGGTGGTGGATTGGATTACCGATGAAGCCCACAAGCTGGTTCCTGGGC
CAGTGTATGACGAAAAAGACATAGACTGA

Upstream 100 bases:

>100_bases
GCTTATCGATTTTATTGGTCGCGGCTGGACGCTATCTCTGTTCCTTGTTTGAGACTACCCCCTACACCTGAAAGCCATTT
TTTCATAAAACGTCGAAATC

Downstream 100 bases:

>100_bases
TCGGAGATAACATTTTCTCGCGGGAATGGCTCCGTCACAGCGCTGTGAAATCCTAAATTATCGCGGCCTGTGCAACGGAA
GCTGGCCTGGTGCATTTGCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MPLVEDLHCFVRVAECGSFSIVARENMVSQATIGRRIGTLEDHFKILLFHRSTRKIVLTDAGQSFLVYARRILQEFEAAE
NALGRHSAEPKGRVRVASTNAFGQYLIRGLAQFNERYPGLHIDLVLGDGFINLIEDGFDLALKIGEVTESSVVARHLGDV
PRSLVASPAYLAKHGAPEDASALQHHECVLFSYGATRQVWNVGGSLVRVSGSFRTNSSIAQFEAVRNGMGISLFPWFQVD
EDVRSGRLIRLLPDMPIEPIHFYMTYPAYRTLPPRTRVVVDWITDEAHKLVPGPVYDEKDID

Sequences:

>Translated_302_residues
MPLVEDLHCFVRVAECGSFSIVARENMVSQATIGRRIGTLEDHFKILLFHRSTRKIVLTDAGQSFLVYARRILQEFEAAE
NALGRHSAEPKGRVRVASTNAFGQYLIRGLAQFNERYPGLHIDLVLGDGFINLIEDGFDLALKIGEVTESSVVARHLGDV
PRSLVASPAYLAKHGAPEDASALQHHECVLFSYGATRQVWNVGGSLVRVSGSFRTNSSIAQFEAVRNGMGISLFPWFQVD
EDVRSGRLIRLLPDMPIEPIHFYMTYPAYRTLPPRTRVVVDWITDEAHKLVPGPVYDEKDID
>Mature_301_residues
PLVEDLHCFVRVAECGSFSIVARENMVSQATIGRRIGTLEDHFKILLFHRSTRKIVLTDAGQSFLVYARRILQEFEAAEN
ALGRHSAEPKGRVRVASTNAFGQYLIRGLAQFNERYPGLHIDLVLGDGFINLIEDGFDLALKIGEVTESSVVARHLGDVP
RSLVASPAYLAKHGAPEDASALQHHECVLFSYGATRQVWNVGGSLVRVSGSFRTNSSIAQFEAVRNGMGISLFPWFQVDE
DVRSGRLIRLLPDMPIEPIHFYMTYPAYRTLPPRTRVVVDWITDEAHKLVPGPVYDEKDID

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=295, Percent_Identity=31.5254237288136, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1789639, Length=290, Percent_Identity=30.6896551724138, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI87081978, Length=289, Percent_Identity=29.4117647058824, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI145693193, Length=290, Percent_Identity=27.9310344827586, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1787128, Length=284, Percent_Identity=27.112676056338, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1789440, Length=301, Percent_Identity=27.5747508305648, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI1787589, Length=279, Percent_Identity=26.5232974910394, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788706, Length=303, Percent_Identity=26.0726072607261, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1786448, Length=285, Percent_Identity=24.5614035087719, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1789173, Length=270, Percent_Identity=25.1851851851852, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI145693105, Length=154, Percent_Identity=29.8701298701299, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI157672245, Length=123, Percent_Identity=39.0243902439024, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786713, Length=272, Percent_Identity=28.6764705882353, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788748, Length=318, Percent_Identity=26.1006289308176, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33747; Mature: 33616

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLVEDLHCFVRVAECGSFSIVARENMVSQATIGRRIGTLEDHFKILLFHRSTRKIVLTD
CCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCHHHCEEEEEEECCCCEEEEEE
AGQSFLVYARRILQEFEAAENALGRHSAEPKGRVRVASTNAFGQYLIRGLAQFNERYPGL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCE
HIDLVLGDGFINLIEDGFDLALKIGEVTESSVVARHLGDVPRSLVASPAYLAKHGAPEDA
EEEEEECCCHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCHHHHCCCHHHHHCCCCCCH
SALQHHECVLFSYGATRQVWNVGGSLVRVSGSFRTNSSIAQFEAVRNGMGISLFPWFQVD
HHHHHCCEEEEECCCCHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCEEEEECEECC
EDVRSGRLIRLLPDMPIEPIHFYMTYPAYRTLPPRTRVVVDWITDEAHKLVPGPVYDEKD
CHHHCCCEEEECCCCCCCCEEEEEECCCCCCCCCCCEEEEEEECCCCHHCCCCCCCCCCC
ID
CC
>Mature Secondary Structure 
PLVEDLHCFVRVAECGSFSIVARENMVSQATIGRRIGTLEDHFKILLFHRSTRKIVLTD
CCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCHHHCEEEEEEECCCCEEEEEE
AGQSFLVYARRILQEFEAAENALGRHSAEPKGRVRVASTNAFGQYLIRGLAQFNERYPGL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCE
HIDLVLGDGFINLIEDGFDLALKIGEVTESSVVARHLGDVPRSLVASPAYLAKHGAPEDA
EEEEEECCCHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCHHHHCCCHHHHHCCCCCCH
SALQHHECVLFSYGATRQVWNVGGSLVRVSGSFRTNSSIAQFEAVRNGMGISLFPWFQVD
HHHHHCCEEEEECCCCHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCEEEEECEECC
EDVRSGRLIRLLPDMPIEPIHFYMTYPAYRTLPPRTRVVVDWITDEAHKLVPGPVYDEKD
CHHHCCCEEEECCCCCCCCEEEEEECCCCCCCCCCCEEEEEEECCCCHHCCCCCCCCCCC
ID
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]