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The map label for this gene is gacS [H]

Identifier: 182680138

GI number: 182680138

Start: 3679143

End: 3681086

Strand: Reverse

Name: gacS [H]

Synonym: Bind_3235

Alternate gene names: 182680138

Gene position: 3681086-3679143 (Counterclockwise)

Preceding gene: 182680142

Following gene: 182680137

Centisome position: 88.27

GC content: 61.11

Gene sequence:

>1944_bases
ATGACCGGGCCTTCGCATATCGATGATTTCATGACCATCTTTCTCGCTGTCGTCGTTTGCGGCGGCGGCGGTGCGCTGTT
TATGGGAACTGTTTTCGCGATTATTAGGCAGGCGAGCGAGCAGAATAGGCGTGTCGCTCTACCCCAGTTAGTCCCTGACG
CGCTTCCAAGCCCCTTGTTGCCGATGGCTGCCAACAAGGGTGACAGGGAAACATGCGCTGCATCGGATAGGGGCGAGGAA
GACCGGGATTGGGCGCGGGAGGAAGCCTGGCAGGATGCCGTCTGGGAAAGCCAGTGCGCGCAGGCCCAAGAAGAACAGGC
CGAGGCCACTCACGCGGCCAAGACGGAATTCTTCGCCAAGATCAGCCATGAGATCCGCACGCCCATCAACGGCATCCTTG
GCTTGACCGAACTTTTGCTCGCCACTGGCCTGACGCCTGAGCAAAAAAACTATGTTGAGGCGATCCGGGGCTCAGGCTCG
GCCCTCGCCTCCCTGACCGATGCGCTGTTGGATTTTTCCAAAATCGAAGCCGGCAAGATGATTCTCGCCGAGGAGCCGTT
CGGCATCTCTCCCCTCGTCGAGGGTGTGACCGAATTGCTTGCGCCCAAGGCCCAGGGCAAGGGATTGGATATTGCCAGTT
CCATCGCGCTCGACGTGCCGGAAACGCTCGTTGGCGATCCTTTGCGCCTGCGTCAGGTTCTGCTCAATCTTGCCGGTAAT
GCGGTCAAATTCACCGAGCAGGGCGGCATTGGGGTGAAAGTGTTTCGCGAGAGCGCCGATAGCCTGTGTTTCGAGGTGAT
CGATACGGGACCGGGTGTGCCCCCTACTTGCCGCGAAACCATTTTCCAGGATTTTGAACAAGGGACTCAAAAGGGGTGCG
AAAGCGGCACGGGCCTCGGTCTTGCCATTTCGAAACGGCTCGTGGAGCGCATGGGCGGCTCTCTCCATCTCGCGTCGCCT
CTAGAGGGTGGGGCCCTGTTTTCCTTCTCTCTACCGCTGCGCGTGGAGGGCGAGCCTCAGCCCTTGCGGGTCCGGCCGGG
CCGTTATGCCGGGCATGCGGTTGTGATCGTTACCGACGGCCCCTTTACCGGCGCCGCTCTTCGCGAACGTTTGATCGAGG
CTGGCGCTGATGCCCGCCTCGCCACGTCGGAAAGCGCGGCGTTGGCTCTTCTGGCACAAGGGGGCGAGGGGGACTTTCGT
CCGGATTTTCTCATCGCAGACATCAGCCTTGGAGCGGACCTGGCCCGCCGTCTGGTCGAGACCGGAGCGAGGGCGGGGCT
CGCTCATTCCCTCCTCATGTTTTCGCCTTTCGAACGGCAAGCCCTGCGGGGCGGCTTGGTGCCGGGTTATGACGGCTGGC
TGATTAAGCCGGTGCGCGCCGCCTCACTGTTTGCCTGCCTCGAACGCTGTCTTGACGATTGTCAGGCTTGCGCGACGAAA
GACTGCGCCGTGACGCCCGCGCCGCCGCGCGTGCTGCTCGCCGAGGATAATGATATCAGTGCTCTGCTCACTACCCGTTA
TCTCGAAAGTTTGGGCGTCGCGGTGGAGCGCGTGGGGGACGGGCTCACGGCGCTCGAGCGTTTCCTGCGGACCTGCGATG
GCGATACTTTGCCTTTTATCGCTTTGTTCCTCGATATCCGCATGCCGGGGCTTGATGGTCTCGAAACCGCCCGCCGCATA
CGTCAAGCCGAGCATCTCATCGCGGCGAAGCCGAATGTTCTGGTTGCGATGAGCGCCAATGGCCTGGAGGAAGACAAACA
GGCCGCGCTCGCCGCCGGTTTCGACCGCTTTTTGCCGAAACCCTTGACCTTTCCCCAGATGCAGACCCTCATTCAGGCGT
TGTTGGCGGAGGAGCCGACAGAACAATCTGTCCCATCGCGCAATCCTCTCATGCCGGTTTGGCCGCAATGGCGCCGTCGG
AAGATCGATCAAAAAGTCGCTTAA

Upstream 100 bases:

>100_bases
TACGATCATTTTTCATCTTTTGCACAAGGAAATCACAAGCCTTCCGCTCGTAAAATCATACGCAATATTCCGGAAGCATC
GGAAGAAAGATTGCGTCATT

Downstream 100 bases:

>100_bases
TGGTTCCGTCGAAGGTAGGCACGAAGACGTGAACCCCAAAGGACGCCAGAGGCCAATAAAGGCAAGCCTTTGGTTTCTCT
CTGGAGCCTCATTAAGCTTT

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 647; Mature: 646

Protein sequence:

>647_residues
MTGPSHIDDFMTIFLAVVVCGGGGALFMGTVFAIIRQASEQNRRVALPQLVPDALPSPLLPMAANKGDRETCAASDRGEE
DRDWAREEAWQDAVWESQCAQAQEEQAEATHAAKTEFFAKISHEIRTPINGILGLTELLLATGLTPEQKNYVEAIRGSGS
ALASLTDALLDFSKIEAGKMILAEEPFGISPLVEGVTELLAPKAQGKGLDIASSIALDVPETLVGDPLRLRQVLLNLAGN
AVKFTEQGGIGVKVFRESADSLCFEVIDTGPGVPPTCRETIFQDFEQGTQKGCESGTGLGLAISKRLVERMGGSLHLASP
LEGGALFSFSLPLRVEGEPQPLRVRPGRYAGHAVVIVTDGPFTGAALRERLIEAGADARLATSESAALALLAQGGEGDFR
PDFLIADISLGADLARRLVETGARAGLAHSLLMFSPFERQALRGGLVPGYDGWLIKPVRAASLFACLERCLDDCQACATK
DCAVTPAPPRVLLAEDNDISALLTTRYLESLGVAVERVGDGLTALERFLRTCDGDTLPFIALFLDIRMPGLDGLETARRI
RQAEHLIAAKPNVLVAMSANGLEEDKQAALAAGFDRFLPKPLTFPQMQTLIQALLAEEPTEQSVPSRNPLMPVWPQWRRR
KIDQKVA

Sequences:

>Translated_647_residues
MTGPSHIDDFMTIFLAVVVCGGGGALFMGTVFAIIRQASEQNRRVALPQLVPDALPSPLLPMAANKGDRETCAASDRGEE
DRDWAREEAWQDAVWESQCAQAQEEQAEATHAAKTEFFAKISHEIRTPINGILGLTELLLATGLTPEQKNYVEAIRGSGS
ALASLTDALLDFSKIEAGKMILAEEPFGISPLVEGVTELLAPKAQGKGLDIASSIALDVPETLVGDPLRLRQVLLNLAGN
AVKFTEQGGIGVKVFRESADSLCFEVIDTGPGVPPTCRETIFQDFEQGTQKGCESGTGLGLAISKRLVERMGGSLHLASP
LEGGALFSFSLPLRVEGEPQPLRVRPGRYAGHAVVIVTDGPFTGAALRERLIEAGADARLATSESAALALLAQGGEGDFR
PDFLIADISLGADLARRLVETGARAGLAHSLLMFSPFERQALRGGLVPGYDGWLIKPVRAASLFACLERCLDDCQACATK
DCAVTPAPPRVLLAEDNDISALLTTRYLESLGVAVERVGDGLTALERFLRTCDGDTLPFIALFLDIRMPGLDGLETARRI
RQAEHLIAAKPNVLVAMSANGLEEDKQAALAAGFDRFLPKPLTFPQMQTLIQALLAEEPTEQSVPSRNPLMPVWPQWRRR
KIDQKVA
>Mature_646_residues
TGPSHIDDFMTIFLAVVVCGGGGALFMGTVFAIIRQASEQNRRVALPQLVPDALPSPLLPMAANKGDRETCAASDRGEED
RDWAREEAWQDAVWESQCAQAQEEQAEATHAAKTEFFAKISHEIRTPINGILGLTELLLATGLTPEQKNYVEAIRGSGSA
LASLTDALLDFSKIEAGKMILAEEPFGISPLVEGVTELLAPKAQGKGLDIASSIALDVPETLVGDPLRLRQVLLNLAGNA
VKFTEQGGIGVKVFRESADSLCFEVIDTGPGVPPTCRETIFQDFEQGTQKGCESGTGLGLAISKRLVERMGGSLHLASPL
EGGALFSFSLPLRVEGEPQPLRVRPGRYAGHAVVIVTDGPFTGAALRERLIEAGADARLATSESAALALLAQGGEGDFRP
DFLIADISLGADLARRLVETGARAGLAHSLLMFSPFERQALRGGLVPGYDGWLIKPVRAASLFACLERCLDDCQACATKD
CAVTPAPPRVLLAEDNDISALLTTRYLESLGVAVERVGDGLTALERFLRTCDGDTLPFIALFLDIRMPGLDGLETARRIR
QAEHLIAAKPNVLVAMSANGLEEDKQAALAAGFDRFLPKPLTFPQMQTLIQALLAEEPTEQSVPSRNPLMPVWPQWRRRK
IDQKVA

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=551, Percent_Identity=28.8566243194192, Blast_Score=209, Evalue=5e-55,
Organism=Escherichia coli, GI87081816, Length=341, Percent_Identity=38.1231671554252, Blast_Score=184, Evalue=2e-47,
Organism=Escherichia coli, GI48994928, Length=457, Percent_Identity=30.4157549234136, Blast_Score=172, Evalue=8e-44,
Organism=Escherichia coli, GI145693157, Length=517, Percent_Identity=29.9806576402321, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI1788713, Length=356, Percent_Identity=30.0561797752809, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1786912, Length=242, Percent_Identity=33.8842975206612, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI87082128, Length=228, Percent_Identity=32.4561403508772, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1788549, Length=244, Percent_Identity=27.4590163934426, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1786600, Length=236, Percent_Identity=27.9661016949153, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1788393, Length=230, Percent_Identity=27.8260869565217, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1789808, Length=223, Percent_Identity=30.0448430493274, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI1790436, Length=224, Percent_Identity=28.5714285714286, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1790300, Length=259, Percent_Identity=26.6409266409266, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1788279, Length=224, Percent_Identity=24.5535714285714, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1790346, Length=232, Percent_Identity=29.7413793103448, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1786783, Length=234, Percent_Identity=27.3504273504274, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1790861, Length=221, Percent_Identity=25.7918552036199, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322044, Length=138, Percent_Identity=33.3333333333333, Blast_Score=68, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 69411; Mature: 69280

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGPSHIDDFMTIFLAVVVCGGGGALFMGTVFAIIRQASEQNRRVALPQLVPDALPSPLL
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEECHHHHHHHCCCCCC
PMAANKGDRETCAASDRGEEDRDWAREEAWQDAVWESQCAQAQEEQAEATHAAKTEFFAK
CHHCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISHEIRTPINGILGLTELLLATGLTPEQKNYVEAIRGSGSALASLTDALLDFSKIEAGKM
HHHHHHCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCE
ILAEEPFGISPLVEGVTELLAPKAQGKGLDIASSIALDVPETLVGDPLRLRQVLLNLAGN
EEECCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCHHHCCCHHHHHHHHHHHCCC
AVKFTEQGGIGVKVFRESADSLCFEVIDTGPGVPPTCRETIFQDFEQGTQKGCESGTGLG
EEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCH
LAISKRLVERMGGSLHLASPLEGGALFSFSLPLRVEGEPQPLRVRPGRYAGHAVVIVTDG
HHHHHHHHHHCCCCEEECCCCCCCEEEEEECCEEECCCCCCEEECCCCCCCCEEEEEECC
PFTGAALRERLIEAGADARLATSESAALALLAQGGEGDFRPDFLIADISLGADLARRLVE
CCCHHHHHHHHHHCCCCCEEECCCCCEEEEEEECCCCCCCCCEEEEEHHHCHHHHHHHHH
TGARAGLAHSLLMFSPFERQALRGGLVPGYDGWLIKPVRAASLFACLERCLDDCQACATK
HHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHCCC
DCAVTPAPPRVLLAEDNDISALLTTRYLESLGVAVERVGDGLTALERFLRTCDGDTLPFI
CCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHH
ALFLDIRMPGLDGLETARRIRQAEHLIAAKPNVLVAMSANGLEEDKQAALAAGFDRFLPK
HHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCC
PLTFPQMQTLIQALLAEEPTEQSVPSRNPLMPVWPQWRRRKIDQKVA
CCCCHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
TGPSHIDDFMTIFLAVVVCGGGGALFMGTVFAIIRQASEQNRRVALPQLVPDALPSPLL
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEECHHHHHHHCCCCCC
PMAANKGDRETCAASDRGEEDRDWAREEAWQDAVWESQCAQAQEEQAEATHAAKTEFFAK
CHHCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISHEIRTPINGILGLTELLLATGLTPEQKNYVEAIRGSGSALASLTDALLDFSKIEAGKM
HHHHHHCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCE
ILAEEPFGISPLVEGVTELLAPKAQGKGLDIASSIALDVPETLVGDPLRLRQVLLNLAGN
EEECCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCHHHCCCHHHHHHHHHHHCCC
AVKFTEQGGIGVKVFRESADSLCFEVIDTGPGVPPTCRETIFQDFEQGTQKGCESGTGLG
EEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCH
LAISKRLVERMGGSLHLASPLEGGALFSFSLPLRVEGEPQPLRVRPGRYAGHAVVIVTDG
HHHHHHHHHHCCCCEEECCCCCCCEEEEEECCEEECCCCCCEEECCCCCCCCEEEEEECC
PFTGAALRERLIEAGADARLATSESAALALLAQGGEGDFRPDFLIADISLGADLARRLVE
CCCHHHHHHHHHHCCCCCEEECCCCCEEEEEEECCCCCCCCCEEEEEHHHCHHHHHHHHH
TGARAGLAHSLLMFSPFERQALRGGLVPGYDGWLIKPVRAASLFACLERCLDDCQACATK
HHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHCCC
DCAVTPAPPRVLLAEDNDISALLTTRYLESLGVAVERVGDGLTALERFLRTCDGDTLPFI
CCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHH
ALFLDIRMPGLDGLETARRIRQAEHLIAAKPNVLVAMSANGLEEDKQAALAAGFDRFLPK
HHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCC
PLTFPQMQTLIQALLAEEPTEQSVPSRNPLMPVWPQWRRRKIDQKVA
CCCCHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]