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The map label for this gene is pnp

Identifier: 182680095

GI number: 182680095

Start: 3631021

End: 3633168

Strand: Reverse

Name: pnp

Synonym: Bind_3192

Alternate gene names: 182680095

Gene position: 3633168-3631021 (Counterclockwise)

Preceding gene: 182680096

Following gene: 182680094

Centisome position: 87.12

GC content: 59.82

Gene sequence:

>2148_bases
ATGCATATGTTCGAGATTCATCGCGAGGAAATCGAGTGGGCCGGGCGCAAGCTCGTGCTGGAGACAGGCAAGATCGCCCG
CCAGGCGGACGGTGCCATTTTCGCGACTTATGGGGAAACCACGGTGCTGGCCACCGTCGTCTCCGCCAAATCGGTCAAGC
CGGGAGTCGATTTCTTTCCGCTGACCGTGAATTATCAGGAAAAAGCTTTCGCCGCGGGCCGGATTCCCGGCGGTTATTTC
AAGCGCGAAGGCCGCCCCTCGGAGCGGGAGACATTGGTTTCCCGCCTGATCGACCGGCCGATCCGTCCCCTCTTTCCCGA
GGGATATCGGAACGAGACGCAGGTGATCGTGACCGTGCTCGCCCATGATCTCGAGAACGATCCCGATATTCTTTCCATGG
TCGCGAGCTCGGCGGCCTTGACGCTCTCGGGCGTTCCCTTCATGGGTCCGGTGGGCGCTGCCCGTGTCGCCTATGTGAAC
GGTCAATATAAGATCAATCCAACCCTTGATGAGGTAAAGGATACGGCGCTCGATCTCGTTGTCGCCGGCACCAGCGATGC
CGTGCTGATGGTCGAATCGGAGGCCAAGGAATTATCCGAGGACGTCATGCTCGGTGCCGTCATGGCGGGCCATAGCTCCT
TCCAGCCGGTCATCGATGCGATCATTCGGCTCGCCGAGAAGGCAGCCAAGGAGCCGCGCGATCTGATCCTGCCCGATAAG
ACCGAGGTCGAGGTGGCAGTGCTTGCGATTGCCGAGAATGATCTCCGCGAAGCCTATAAAATCACCTCCAAGCAGGATCG
CTATGCGGCGGTCGATGCGGTGAAGGCAAAGGTCTTCGGGGATCTGCTGCCGGCCGAGGGCGAGGCGAAATTTCCCAAGG
ATCTCGTCGCCGAGGTGTTCCACGATCTTCAGGCCAAGGTCGTGCGCTGGAACATTCTCGATAATGGTATTCGCATCGAC
GGGCGTGATGTGAAAACCGTGCGTCCGATCCTGGCGGAGGTTGGCATTCTGCCGCGTGCTCACGGCTCGGCTTTGTTCAC
ACGCGGTGAGACCCAGGCGCTTGTCGTCGCGACGCTCGGGACCGGCGAGGACGAGCAATTGATCGACAATCTGGAGGGCA
CCTATCGGGAGCGCTTCCTGCTGCATTATAATTTCCCGCCCTATTCGGTCGGTGAGGCGGGCCGTATGGGCTCGCCTGGA
CGCCGCGAGATCGGCCATGGCAAGCTCGCCTGGCGCGCAATCCATCCGATGCTGCCGACGCCGGCAGAATTCCCTTATAC
GATCCGCGTCGTTTCGGAGATTACCGAATCCAATGGTTCGTCCTCCATGGCCACGGTCTGCGGCTCCTCTTTGGCTTTGA
TGGATGCCGGCGTGCCCTTGAAGCGCCCGACGGCAGGCATTGCCATGGGTCTGATCCTTGAAGGCGAGCGTTATGCGGTC
CTGTCCGATATTCTCGGCGACGAGGATCATCTGGGCGACATGGATTTCAAGGTCGCCGGCACCGAGGAGGGCATTACCTC
GTTGCAGATGGACATCAAGATCACGGGCATCAACGAAGAGATCATGCGGGTGGCGCTGGGCCAGGCCAAGGAAGGCCGCC
TGCATATTCTCGGCGAAATGGCCAAGGCTATCACGGGGGCGAGAGCAGAACTCGGCGAATTCGCGCCCCGCATCGAGACC
CTGCGCATTCCGACCGAAAAGATCCGGGAAGTCATCGGTACGGGCGGCAAGGTTATCCGTGAGATCTGCGAGAAAACCGG
TGCCAAGATCAATATCGAGGATGACGGTACGGTGAAGGTCGCCTCCTCCGACGGTAATTCGATCAAGGCGGCGATCAATT
GGATCAAGTCGATCGCCACCGATCCGGAAGTTGGCCATATTTACGACGGCACGGTGGTCAAGGTCGTCGATTTCGGCGCC
TTCGTGAATTTCTTCGGCTCGAAGGACGGCCTCGTGCATATTTCGCAGCTCGCGAAGGGGCGCGTGGCGAAAACCTCCGA
TGTCGTCAAGGAAGGCGATAAGGTGAAGGTCAAGCTGCTCGGATTCGATGATCGCGGCAAGGTGCGCCTGTCCATGCGTC
TCGTCGATCAGGAAACCGGCGAGGATCTCGAAGGCAAGGAGCCGCCGCAAGCCGAAGCTGGCGAATAA

Upstream 100 bases:

>100_bases
TTGCTCGTGGCAGCTTCTCGCCGTCTTGCTCGTGGCTTTCGTCTTGGTCGATTTCATTCAATCCATCTTTTGTACAAGAC
AAGTCACTTAAGAAAGACGC

Downstream 100 bases:

>100_bases
GCCTCATTCTTGCTTGTTATCCGGCCGCGTTCGACAAGGACGCGGCCTTTTTCATGTAAAGCCCTCATGCCCAATTCTCT
CGCTCCCGAAAATCGCGTCA

Product: polyribonucleotide nucleotidyltransferase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 715; Mature: 715

Protein sequence:

>715_residues
MHMFEIHREEIEWAGRKLVLETGKIARQADGAIFATYGETTVLATVVSAKSVKPGVDFFPLTVNYQEKAFAAGRIPGGYF
KREGRPSERETLVSRLIDRPIRPLFPEGYRNETQVIVTVLAHDLENDPDILSMVASSAALTLSGVPFMGPVGAARVAYVN
GQYKINPTLDEVKDTALDLVVAGTSDAVLMVESEAKELSEDVMLGAVMAGHSSFQPVIDAIIRLAEKAAKEPRDLILPDK
TEVEVAVLAIAENDLREAYKITSKQDRYAAVDAVKAKVFGDLLPAEGEAKFPKDLVAEVFHDLQAKVVRWNILDNGIRID
GRDVKTVRPILAEVGILPRAHGSALFTRGETQALVVATLGTGEDEQLIDNLEGTYRERFLLHYNFPPYSVGEAGRMGSPG
RREIGHGKLAWRAIHPMLPTPAEFPYTIRVVSEITESNGSSSMATVCGSSLALMDAGVPLKRPTAGIAMGLILEGERYAV
LSDILGDEDHLGDMDFKVAGTEEGITSLQMDIKITGINEEIMRVALGQAKEGRLHILGEMAKAITGARAELGEFAPRIET
LRIPTEKIREVIGTGGKVIREICEKTGAKINIEDDGTVKVASSDGNSIKAAINWIKSIATDPEVGHIYDGTVVKVVDFGA
FVNFFGSKDGLVHISQLAKGRVAKTSDVVKEGDKVKVKLLGFDDRGKVRLSMRLVDQETGEDLEGKEPPQAEAGE

Sequences:

>Translated_715_residues
MHMFEIHREEIEWAGRKLVLETGKIARQADGAIFATYGETTVLATVVSAKSVKPGVDFFPLTVNYQEKAFAAGRIPGGYF
KREGRPSERETLVSRLIDRPIRPLFPEGYRNETQVIVTVLAHDLENDPDILSMVASSAALTLSGVPFMGPVGAARVAYVN
GQYKINPTLDEVKDTALDLVVAGTSDAVLMVESEAKELSEDVMLGAVMAGHSSFQPVIDAIIRLAEKAAKEPRDLILPDK
TEVEVAVLAIAENDLREAYKITSKQDRYAAVDAVKAKVFGDLLPAEGEAKFPKDLVAEVFHDLQAKVVRWNILDNGIRID
GRDVKTVRPILAEVGILPRAHGSALFTRGETQALVVATLGTGEDEQLIDNLEGTYRERFLLHYNFPPYSVGEAGRMGSPG
RREIGHGKLAWRAIHPMLPTPAEFPYTIRVVSEITESNGSSSMATVCGSSLALMDAGVPLKRPTAGIAMGLILEGERYAV
LSDILGDEDHLGDMDFKVAGTEEGITSLQMDIKITGINEEIMRVALGQAKEGRLHILGEMAKAITGARAELGEFAPRIET
LRIPTEKIREVIGTGGKVIREICEKTGAKINIEDDGTVKVASSDGNSIKAAINWIKSIATDPEVGHIYDGTVVKVVDFGA
FVNFFGSKDGLVHISQLAKGRVAKTSDVVKEGDKVKVKLLGFDDRGKVRLSMRLVDQETGEDLEGKEPPQAEAGE
>Mature_715_residues
MHMFEIHREEIEWAGRKLVLETGKIARQADGAIFATYGETTVLATVVSAKSVKPGVDFFPLTVNYQEKAFAAGRIPGGYF
KREGRPSERETLVSRLIDRPIRPLFPEGYRNETQVIVTVLAHDLENDPDILSMVASSAALTLSGVPFMGPVGAARVAYVN
GQYKINPTLDEVKDTALDLVVAGTSDAVLMVESEAKELSEDVMLGAVMAGHSSFQPVIDAIIRLAEKAAKEPRDLILPDK
TEVEVAVLAIAENDLREAYKITSKQDRYAAVDAVKAKVFGDLLPAEGEAKFPKDLVAEVFHDLQAKVVRWNILDNGIRID
GRDVKTVRPILAEVGILPRAHGSALFTRGETQALVVATLGTGEDEQLIDNLEGTYRERFLLHYNFPPYSVGEAGRMGSPG
RREIGHGKLAWRAIHPMLPTPAEFPYTIRVVSEITESNGSSSMATVCGSSLALMDAGVPLKRPTAGIAMGLILEGERYAV
LSDILGDEDHLGDMDFKVAGTEEGITSLQMDIKITGINEEIMRVALGQAKEGRLHILGEMAKAITGARAELGEFAPRIET
LRIPTEKIREVIGTGGKVIREICEKTGAKINIEDDGTVKVASSDGNSIKAAINWIKSIATDPEVGHIYDGTVVKVVDFGA
FVNFFGSKDGLVHISQLAKGRVAKTSDVVKEGDKVKVKLLGFDDRGKVRLSMRLVDQETGEDLEGKEPPQAEAGE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=721, Percent_Identity=37.4479889042996, Blast_Score=441, Evalue=1e-123,
Organism=Homo sapiens, GI4826690, Length=87, Percent_Identity=51.7241379310345, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI21361576, Length=87, Percent_Identity=47.1264367816092, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI145693187, Length=686, Percent_Identity=57.8717201166181, Blast_Score=783, Evalue=0.0,
Organism=Escherichia coli, GI87082262, Length=96, Percent_Identity=44.7916666666667, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI115534063, Length=720, Percent_Identity=34.0277777777778, Blast_Score=361, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17535281, Length=88, Percent_Identity=47.7272727272727, Blast_Score=79, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6320850, Length=98, Percent_Identity=35.7142857142857, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI281362905, Length=710, Percent_Identity=37.7464788732394, Blast_Score=448, Evalue=1e-126,
Organism=Drosophila melanogaster, GI24651641, Length=710, Percent_Identity=37.7464788732394, Blast_Score=448, Evalue=1e-126,
Organism=Drosophila melanogaster, GI24651643, Length=710, Percent_Identity=37.7464788732394, Blast_Score=448, Evalue=1e-126,
Organism=Drosophila melanogaster, GI161079377, Length=661, Percent_Identity=37.6701966717095, Blast_Score=410, Evalue=1e-114,
Organism=Drosophila melanogaster, GI20129977, Length=91, Percent_Identity=47.2527472527472, Blast_Score=79, Evalue=1e-14,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_BEII9 (B2ICY4)

Other databases:

- EMBL:   CP001016
- RefSeq:   YP_001834241.1
- GeneID:   6198522
- GenomeReviews:   CP001016_GR
- KEGG:   bid:Bind_3192
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   CLSK2405849
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 77500; Mature: 77500

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHMFEIHREEIEWAGRKLVLETGKIARQADGAIFATYGETTVLATVVSAKSVKPGVDFFP
CCCHHHHHHHHHHCCCEEEEECCHHHHHCCCCEEEECCCCEEEHHHHHHCCCCCCCCEEE
LTVNYQEKAFAAGRIPGGYFKREGRPSERETLVSRLIDRPIRPLFPEGYRNETQVIVTVL
EEECCCHHHHCCCCCCCCHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHEEH
AHDLENDPDILSMVASSAALTLSGVPFMGPVGAARVAYVNGQYKINPTLDEVKDTALDLV
HHHCCCCHHHHHHHHHCCEEEECCCCCCCCCCCCEEEEECCEEEECCCHHHHHHHHEEEE
VAGTSDAVLMVESEAKELSEDVMLGAVMAGHSSFQPVIDAIIRLAEKAAKEPRDLILPDK
EECCCCEEEEEECHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
TEVEVAVLAIAENDLREAYKITSKQDRYAAVDAVKAKVFGDLLPAEGEAKFPKDLVAEVF
CCEEEEEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
HDLQAKVVRWNILDNGIRIDGRDVKTVRPILAEVGILPRAHGSALFTRGETQALVVATLG
HHHHHEEEEEEEECCCEEECCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEEEEEEC
TGEDEQLIDNLEGTYRERFLLHYNFPPYSVGEAGRMGSPGRREIGHGKLAWRAIHPMLPT
CCCHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCC
PAEFPYTIRVVSEITESNGSSSMATVCGSSLALMDAGVPLKRPTAGIAMGLILEGERYAV
CCCCCEEEHHHHHHHHCCCCCCHHHHHCCCEEEEECCCCCCCCCCCEEEEEEECCCCHHH
LSDILGDEDHLGDMDFKVAGTEEGITSLQMDIKITGINEEIMRVALGQAKEGRLHILGEM
HHHHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECCCHHHHHHHHCCCCCCCEEHHHHH
AKAITGARAELGEFAPRIETLRIPTEKIREVIGTGGKVIREICEKTGAKINIEDDGTVKV
HHHHHCCHHHHHHHCCCCEEEECCHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEE
ASSDGNSIKAAINWIKSIATDPEVGHIYDGTVVKVVDFGAFVNFFGSKDGLVHISQLAKG
ECCCCCHHHHHHHHHHHHCCCCCCCEEECCEEEEEEHHHHHHHHHCCCCCEEEHHHHHCC
RVAKTSDVVKEGDKVKVKLLGFDDRGKVRLSMRLVDQETGEDLEGKEPPQAEAGE
CCCCCHHHHHCCCEEEEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MHMFEIHREEIEWAGRKLVLETGKIARQADGAIFATYGETTVLATVVSAKSVKPGVDFFP
CCCHHHHHHHHHHCCCEEEEECCHHHHHCCCCEEEECCCCEEEHHHHHHCCCCCCCCEEE
LTVNYQEKAFAAGRIPGGYFKREGRPSERETLVSRLIDRPIRPLFPEGYRNETQVIVTVL
EEECCCHHHHCCCCCCCCHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHEEH
AHDLENDPDILSMVASSAALTLSGVPFMGPVGAARVAYVNGQYKINPTLDEVKDTALDLV
HHHCCCCHHHHHHHHHCCEEEECCCCCCCCCCCCEEEEECCEEEECCCHHHHHHHHEEEE
VAGTSDAVLMVESEAKELSEDVMLGAVMAGHSSFQPVIDAIIRLAEKAAKEPRDLILPDK
EECCCCEEEEEECHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
TEVEVAVLAIAENDLREAYKITSKQDRYAAVDAVKAKVFGDLLPAEGEAKFPKDLVAEVF
CCEEEEEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
HDLQAKVVRWNILDNGIRIDGRDVKTVRPILAEVGILPRAHGSALFTRGETQALVVATLG
HHHHHEEEEEEEECCCEEECCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEEEEEEC
TGEDEQLIDNLEGTYRERFLLHYNFPPYSVGEAGRMGSPGRREIGHGKLAWRAIHPMLPT
CCCHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCC
PAEFPYTIRVVSEITESNGSSSMATVCGSSLALMDAGVPLKRPTAGIAMGLILEGERYAV
CCCCCEEEHHHHHHHHCCCCCCHHHHHCCCEEEEECCCCCCCCCCCEEEEEEECCCCHHH
LSDILGDEDHLGDMDFKVAGTEEGITSLQMDIKITGINEEIMRVALGQAKEGRLHILGEM
HHHHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECCCHHHHHHHHCCCCCCCEEHHHHH
AKAITGARAELGEFAPRIETLRIPTEKIREVIGTGGKVIREICEKTGAKINIEDDGTVKV
HHHHHCCHHHHHHHCCCCEEEECCHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEE
ASSDGNSIKAAINWIKSIATDPEVGHIYDGTVVKVVDFGAFVNFFGSKDGLVHISQLAKG
ECCCCCHHHHHHHHHHHHCCCCCCCEEECCEEEEEEHHHHHHHHHCCCCCEEEHHHHHCC
RVAKTSDVVKEGDKVKVKLLGFDDRGKVRLSMRLVDQETGEDLEGKEPPQAEAGE
CCCCCHHHHHCCCEEEEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA