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The map label for this gene is nth [H]

Identifier: 182680010

GI number: 182680010

Start: 3553695

End: 3554453

Strand: Reverse

Name: nth [H]

Synonym: Bind_3106

Alternate gene names: 182680010

Gene position: 3554453-3553695 (Counterclockwise)

Preceding gene: 182680012

Following gene: 182680008

Centisome position: 85.24

GC content: 56.65

Gene sequence:

>759_bases
ATGAAGGAGCCTCAGACGCCTGTCTCCGTGCCGCCGGAGGCCGCGCTGACTCCGATCGGCGCCGCGAAGATCCGTGAAAC
CAAAATTCGCGCTTCGGCTAAAACCCGTAAGGCGAAGGCGAAATTGCCGGACCTCGCGGAAGTGGCCGAAATTTTCCGGC
GCTTTGCCGCCGCGGACCCTCATCCGGAGGGCGAACTTTATTCCGTCAATGATTTCACCTTTCTCATCGCCGTCGTGCTT
TCCGCGCAGGCGACGGATGCCGGTGTCAATAAAGCGACGAAAGCGCTTTTCGCCATAGCCGACAGCCCCGAAAAAATGCT
GGCTCTCGGTGAGGATAAACTGCGCGATATGATCAAGACGATCGGTCTCTACCAAGCCAAAGCCAAGAATATCATGGCTT
TATGCGCAAACTTGATCGAGAATTACGGCGGCCAAGTGCCGCATGACCGCGAAGCCCTGCAAAGTCTTGCGGGCGTTGGC
CGTAAAACTGCTAATGTGGTGTTGAATATTGCCTTCGGTGAGCCCACCATCGCGGTGGATACGCATATTTTTCGCGTCTC
GAACCGGATTCCGCTTGCGATCGGCAAAACCCCGCTTGCCGTCGAACAGGGACTCGAGAAGATCGTCCCGCCCGAATATA
AACTCCATGCCCACGTCTGGCTGATCTTGCATGGACGCCATGTCTGCAAGGCTCGGCGGCCGGAATGCGAACGCTGCATT
ATCTCCGATCTATGCCATTCGCCGGAAAAGCGGGTTTGA

Upstream 100 bases:

>100_bases
TTGAGAAGGAACCAAGGGCAGCTCTTCGCAATTTGCAACTCTTGGTATAACGGGCATGGCAAGGAAAAAGCAGTTCCAGT
CTTCAGAGGCTTCAGTCGAT

Downstream 100 bases:

>100_bases
TGGTCGCCGCACGAAGGTTGACCCAGCAGAGGTGATCAAAGCGGGCCTTGATGCCTTGCAGCCATTGCGGGGCCCCATCG
TCATGGATTGGCTCGACTGC

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MKEPQTPVSVPPEAALTPIGAAKIRETKIRASAKTRKAKAKLPDLAEVAEIFRRFAAADPHPEGELYSVNDFTFLIAVVL
SAQATDAGVNKATKALFAIADSPEKMLALGEDKLRDMIKTIGLYQAKAKNIMALCANLIENYGGQVPHDREALQSLAGVG
RKTANVVLNIAFGEPTIAVDTHIFRVSNRIPLAIGKTPLAVEQGLEKIVPPEYKLHAHVWLILHGRHVCKARRPECERCI
ISDLCHSPEKRV

Sequences:

>Translated_252_residues
MKEPQTPVSVPPEAALTPIGAAKIRETKIRASAKTRKAKAKLPDLAEVAEIFRRFAAADPHPEGELYSVNDFTFLIAVVL
SAQATDAGVNKATKALFAIADSPEKMLALGEDKLRDMIKTIGLYQAKAKNIMALCANLIENYGGQVPHDREALQSLAGVG
RKTANVVLNIAFGEPTIAVDTHIFRVSNRIPLAIGKTPLAVEQGLEKIVPPEYKLHAHVWLILHGRHVCKARRPECERCI
ISDLCHSPEKRV
>Mature_252_residues
MKEPQTPVSVPPEAALTPIGAAKIRETKIRASAKTRKAKAKLPDLAEVAEIFRRFAAADPHPEGELYSVNDFTFLIAVVL
SAQATDAGVNKATKALFAIADSPEKMLALGEDKLRDMIKTIGLYQAKAKNIMALCANLIENYGGQVPHDREALQSLAGVG
RKTANVVLNIAFGEPTIAVDTHIFRVSNRIPLAIGKTPLAVEQGLEKIVPPEYKLHAHVWLILHGRHVCKARRPECERCI
ISDLCHSPEKRV

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=180, Percent_Identity=30, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1787920, Length=201, Percent_Identity=57.2139303482587, Blast_Score=233, Evalue=1e-62,
Organism=Caenorhabditis elegans, GI17554540, Length=181, Percent_Identity=33.7016574585635, Blast_Score=94, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6324530, Length=194, Percent_Identity=27.319587628866, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI45550361, Length=179, Percent_Identity=28.4916201117318, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 27502; Mature: 27502

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEPQTPVSVPPEAALTPIGAAKIRETKIRASAKTRKAKAKLPDLAEVAEIFRRFAAADP
CCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCC
HPEGELYSVNDFTFLIAVVLSAQATDAGVNKATKALFAIADSPEKMLALGEDKLRDMIKT
CCCCCEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHH
IGLYQAKAKNIMALCANLIENYGGQVPHDREALQSLAGVGRKTANVVLNIAFGEPTIAVD
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEEEEECCCEEEEC
THIFRVSNRIPLAIGKTPLAVEQGLEKIVPPEYKLHAHVWLILHGRHVCKARRPECERCI
CEEEEECCCCCEEECCCCHHHHHHHHHHCCCCEEEEEEEEEEEECCHHHHCCCCHHHHHH
ISDLCHSPEKRV
HHHHHCCCCCCC
>Mature Secondary Structure
MKEPQTPVSVPPEAALTPIGAAKIRETKIRASAKTRKAKAKLPDLAEVAEIFRRFAAADP
CCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCC
HPEGELYSVNDFTFLIAVVLSAQATDAGVNKATKALFAIADSPEKMLALGEDKLRDMIKT
CCCCCEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHH
IGLYQAKAKNIMALCANLIENYGGQVPHDREALQSLAGVGRKTANVVLNIAFGEPTIAVD
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEEEEECCCEEEEC
THIFRVSNRIPLAIGKTPLAVEQGLEKIVPPEYKLHAHVWLILHGRHVCKARRPECERCI
CEEEEECCCCCEEECCCCHHHHHHHHHHCCCCEEEEEEEEEEEECCHHHHCCCCHHHHHH
ISDLCHSPEKRV
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]