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The map label for this gene is rbsB [H]

Identifier: 182679975

GI number: 182679975

Start: 3506413

End: 3507342

Strand: Reverse

Name: rbsB [H]

Synonym: Bind_3071

Alternate gene names: 182679975

Gene position: 3507342-3506413 (Counterclockwise)

Preceding gene: 182679976

Following gene: 182679974

Centisome position: 84.11

GC content: 56.67

Gene sequence:

>930_bases
ATGAACAAGGTGCTGGTGGCTGCCGCGGCGGTGGCTCTCATGACGGCGCACGCATCGGCGAAAGACCTCAAATCAATCGG
CCTCTCGCTCGCCTCGATGGATAATCCCTATTTCGTCGCCATGGCCAAGGGCGCTACGGCAAGTGCCCAGAAAATCAATC
CGAATGTCAAGATCAATACGCTTGATTTCGATTATGATCTGAACAAACAGGCCACCCAGATCGATAGTCTCATTGCCTCG
GGGGTCGATTTGATCCTGCTCAATCCGGGCGATCCCGCGGCTCTGGAGCCGGCGATCCAACGGGCCCATGCCGCCGGTAT
TCCCGTCATCGCTGTCGATGCAGCAGCCAAAGGCGCCGATATCACCATCACCACGGATAATCGGCAGGCGGGGGAAATCG
CCTGTCAATATATCGTCGACAAGCTCGGCGGCAAGGGCAATGTCATCATTCAGAACGGTCCGCAGGTGTCTTCCGTGCGC
GAGCGTGTTGAGGGCTGCAAGAAGGCGCTTGCGAATGCTCCGGAGATCGCGGTCCTTTCAAGCGATCAAAATGCCAAAGG
ATCGCATCGGCATGGCATGAATGTCATGCAGGACCATCTTTCCCAGTTTGCGAAGATCGATGCGGTCTTCACGATCAATG
ACCGGCAGGCGATTGGCTCTGATTTTGCCGCGCAGCGCCTCCATCGCAACGATCTCATCATTACTTCGGTCGATGGCGCG
CCTGATATCGAGGTCGCTCTCAAGGCGAAGAACACCTGCATCCATGCCTCGGCCAGCCAAGATCCCTATGCGATTGCCCA
GCTTGCCGTTGATCTCGGCAATGATCTTCTGAACGGCAAGAAGCCCGAGCAGGATGTCATTCTCATCGCGCCGAAACTGG
TCACGCGTGAGAATGTCGCTGAATACAAGGGTTGGCGGGCCGAGCGTTAA

Upstream 100 bases:

>100_bases
AGCTGATTGCCTCTCCGTCATTTCCGTGAGCTATATAAAGCGTTTCTCATTTTGCCCATGAACGTGGCGAGAGAGTTAAT
AAGCAAGGGAGGAAATGAAG

Downstream 100 bases:

>100_bases
CCAGCGCACCAACATCAACCGCAAAAGGGAGACCTCTTTGCGGTTGATGCGTTTCAAAGAGCTGGAACAGTTGCTCCAGT
ATCCATTCTTATTGAAGCGC

Product: periplasmic binding protein/LacI transcriptional regulator

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MNKVLVAAAAVALMTAHASAKDLKSIGLSLASMDNPYFVAMAKGATASAQKINPNVKINTLDFDYDLNKQATQIDSLIAS
GVDLILLNPGDPAALEPAIQRAHAAGIPVIAVDAAAKGADITITTDNRQAGEIACQYIVDKLGGKGNVIIQNGPQVSSVR
ERVEGCKKALANAPEIAVLSSDQNAKGSHRHGMNVMQDHLSQFAKIDAVFTINDRQAIGSDFAAQRLHRNDLIITSVDGA
PDIEVALKAKNTCIHASASQDPYAIAQLAVDLGNDLLNGKKPEQDVILIAPKLVTRENVAEYKGWRAER

Sequences:

>Translated_309_residues
MNKVLVAAAAVALMTAHASAKDLKSIGLSLASMDNPYFVAMAKGATASAQKINPNVKINTLDFDYDLNKQATQIDSLIAS
GVDLILLNPGDPAALEPAIQRAHAAGIPVIAVDAAAKGADITITTDNRQAGEIACQYIVDKLGGKGNVIIQNGPQVSSVR
ERVEGCKKALANAPEIAVLSSDQNAKGSHRHGMNVMQDHLSQFAKIDAVFTINDRQAIGSDFAAQRLHRNDLIITSVDGA
PDIEVALKAKNTCIHASASQDPYAIAQLAVDLGNDLLNGKKPEQDVILIAPKLVTRENVAEYKGWRAER
>Mature_309_residues
MNKVLVAAAAVALMTAHASAKDLKSIGLSLASMDNPYFVAMAKGATASAQKINPNVKINTLDFDYDLNKQATQIDSLIAS
GVDLILLNPGDPAALEPAIQRAHAAGIPVIAVDAAAKGADITITTDNRQAGEIACQYIVDKLGGKGNVIIQNGPQVSSVR
ERVEGCKKALANAPEIAVLSSDQNAKGSHRHGMNVMQDHLSQFAKIDAVFTINDRQAIGSDFAAQRLHRNDLIITSVDGA
PDIEVALKAKNTCIHASASQDPYAIAQLAVDLGNDLLNGKKPEQDVILIAPKLVTRENVAEYKGWRAER

Specific function: Involved in the high-affinity D-ribose membrane transport system [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=287, Percent_Identity=34.1463414634146, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1790674, Length=260, Percent_Identity=27.3076923076923, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790526, Length=286, Percent_Identity=24.8251748251748, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 32714; Mature: 32714

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKVLVAAAAVALMTAHASAKDLKSIGLSLASMDNPYFVAMAKGATASAQKINPNVKINT
CCCHHHHHHHHHHHHHCCCHHHHHHHCCHHCCCCCCEEEEEECCCCCCHHHCCCCEEEEE
LDFDYDLNKQATQIDSLIASGVDLILLNPGDPAALEPAIQRAHAAGIPVIAVDAAAKGAD
EECCCCCCCHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCE
ITITTDNRQAGEIACQYIVDKLGGKGNVIIQNGPQVSSVRERVEGCKKALANAPEIAVLS
EEEEECCCCHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEE
SDQNAKGSHRHGMNVMQDHLSQFAKIDAVFTINDRQAIGSDFAAQRLHRNDLIITSVDGA
CCCCCCCCCCCCHHHHHHHHHHHHHEEEEEEECCHHHHCCHHHHHHHCCCCEEEEECCCC
PDIEVALKAKNTCIHASASQDPYAIAQLAVDLGNDLLNGKKPEQDVILIAPKLVTRENVA
CCEEEEEEECCEEEECCCCCCCHHHHHHHHHHCHHHHCCCCCCCCEEEECCCHHHHHHHH
EYKGWRAER
HHCCCCCCC
>Mature Secondary Structure
MNKVLVAAAAVALMTAHASAKDLKSIGLSLASMDNPYFVAMAKGATASAQKINPNVKINT
CCCHHHHHHHHHHHHHCCCHHHHHHHCCHHCCCCCCEEEEEECCCCCCHHHCCCCEEEEE
LDFDYDLNKQATQIDSLIASGVDLILLNPGDPAALEPAIQRAHAAGIPVIAVDAAAKGAD
EECCCCCCCHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCE
ITITTDNRQAGEIACQYIVDKLGGKGNVIIQNGPQVSSVRERVEGCKKALANAPEIAVLS
EEEEECCCCHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEE
SDQNAKGSHRHGMNVMQDHLSQFAKIDAVFTINDRQAIGSDFAAQRLHRNDLIITSVDGA
CCCCCCCCCCCCHHHHHHHHHHHHHEEEEEEECCHHHHCCHHHHHHHCCCCEEEEECCCC
PDIEVALKAKNTCIHASASQDPYAIAQLAVDLGNDLLNGKKPEQDVILIAPKLVTRENVA
CCEEEEEEECCEEEECCCCCCCHHHHHHHHHHCHHHHCCCCCCCCEEEECCCHHHHHHHH
EYKGWRAER
HHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 7921236 [H]