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The map label for this gene is thlA [H]

Identifier: 182679963

GI number: 182679963

Start: 3482479

End: 3483663

Strand: Reverse

Name: thlA [H]

Synonym: Bind_3058

Alternate gene names: 182679963

Gene position: 3483663-3482479 (Counterclockwise)

Preceding gene: 182679973

Following gene: 182679957

Centisome position: 83.54

GC content: 60.34

Gene sequence:

>1185_bases
ATGACCCAGCGCGATATTGTTCTGTGCAGTCCGGTTCGGACCGCGATCGGCAATTATAATGGCTCGCTCAAGAACACACC
CGCGACCGAACTTGGCGCCATTGTGGTGCGTGAGACCCTGAAGAGGGCAGGACTCGAATCCAGCGAGATCGGTTCTGTTG
TAATGGGCAATGTCATTCAGGCGGGCAACAGGATGAATCCCGCCCGTCAAGCCGCGATCGGCGGTGGTACGCCGGTCTCC
GTTCCCGCCATGACGGTGAATCGTGTCTGTGGTTCTGGTGCCCAGGCCATTGTCACCGCCGCTCAGGAGATCGCGATAGG
CGAGATCGATGCGGCTGTGGCGGGAGGCATGGAGAATATGGACCGTGCGCCTTATCTGATGGAAGGGGGACGCTGGGGCT
ATCGGATGGGTCCCGCCGAAATCCTCGACGCCATGCTGACGGATGGGCTGAATGATGCCTTTTCGGGTGAGCATTCCGGC
TGGCATACGGAAGATCTCGTCACGCAATTACAGATTACGCGTGAATCACAGGACCGTTTTGCCGTTCGCTCGCAGCAGCG
CTTTTCGGAGGCCCAAAAAGCGGGGCATTTCAAGGACGAAATTGTCGCGGTGGAGGTAAAAGGCAAGAAGGGGCCGGAGC
GCTTCGAATCCGACGAGGCGCCGCGCCCAGATACGACGCTCGAGATCCTGACCAAGCTTAAGCCCGCCTTCCGCAAAGAT
GGGACGATCACCGCTGGTAATGCGCCTGGGCTCAACAGCGGTGCGGCTGCGATGATCGTGGCTGATCGCGCCTTCGCCGA
AGCAAAAGGCGTAACGCCCATGGCGCATCTGGTGGCGTTTGGAGTTGCGGCGGTTGAGCCGGGCTTGTTTGGGCTCGGCC
CGGTGCCAGCCGTCCGCAAGGCGCTTGAGCGGGCTGGCTGGAAACTTTCCGATATCGAGCGGATCGAAATCAATGAAGCC
TTTGCGGCTGTACCGCTTGCCGTCGCGAAAGAGCTTGGCTTGCCGCAGGATATTATCAATGTCGAAGGGGGAGCCGTCGC
GCATGGTCACCCGATTGGTGCGACCGGCGCGGTGCTGACGACCAGGCTTCTCCACTCGATGAAACGCGATGGCCTCAAGC
GAGGAATCGTGACGCTCTGCATTGGGGGAGGGCAGGGGATTGCCCTCGCGTTTGAGGCTGCCTAA

Upstream 100 bases:

>100_bases
AGGTGCACTGCCGTCTCCACAATGACCCATAAGAGCTGGCGTGACCCGATGGGACCGTCGGCAAGAACGAAGTCTTTGGA
GCTTACGGAGGAGACTTGTC

Downstream 100 bases:

>100_bases
TCGGGCGTCCTCTTTGGGTAAGTTTGATGCGGCCATAAAGACAGACAGCATCGCTCCTCGGATCGAGAATCACGGATTCT
CGATCCGTCATTTTTAAATC

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MTQRDIVLCSPVRTAIGNYNGSLKNTPATELGAIVVRETLKRAGLESSEIGSVVMGNVIQAGNRMNPARQAAIGGGTPVS
VPAMTVNRVCGSGAQAIVTAAQEIAIGEIDAAVAGGMENMDRAPYLMEGGRWGYRMGPAEILDAMLTDGLNDAFSGEHSG
WHTEDLVTQLQITRESQDRFAVRSQQRFSEAQKAGHFKDEIVAVEVKGKKGPERFESDEAPRPDTTLEILTKLKPAFRKD
GTITAGNAPGLNSGAAAMIVADRAFAEAKGVTPMAHLVAFGVAAVEPGLFGLGPVPAVRKALERAGWKLSDIERIEINEA
FAAVPLAVAKELGLPQDIINVEGGAVAHGHPIGATGAVLTTRLLHSMKRDGLKRGIVTLCIGGGQGIALAFEAA

Sequences:

>Translated_394_residues
MTQRDIVLCSPVRTAIGNYNGSLKNTPATELGAIVVRETLKRAGLESSEIGSVVMGNVIQAGNRMNPARQAAIGGGTPVS
VPAMTVNRVCGSGAQAIVTAAQEIAIGEIDAAVAGGMENMDRAPYLMEGGRWGYRMGPAEILDAMLTDGLNDAFSGEHSG
WHTEDLVTQLQITRESQDRFAVRSQQRFSEAQKAGHFKDEIVAVEVKGKKGPERFESDEAPRPDTTLEILTKLKPAFRKD
GTITAGNAPGLNSGAAAMIVADRAFAEAKGVTPMAHLVAFGVAAVEPGLFGLGPVPAVRKALERAGWKLSDIERIEINEA
FAAVPLAVAKELGLPQDIINVEGGAVAHGHPIGATGAVLTTRLLHSMKRDGLKRGIVTLCIGGGQGIALAFEAA
>Mature_393_residues
TQRDIVLCSPVRTAIGNYNGSLKNTPATELGAIVVRETLKRAGLESSEIGSVVMGNVIQAGNRMNPARQAAIGGGTPVSV
PAMTVNRVCGSGAQAIVTAAQEIAIGEIDAAVAGGMENMDRAPYLMEGGRWGYRMGPAEILDAMLTDGLNDAFSGEHSGW
HTEDLVTQLQITRESQDRFAVRSQQRFSEAQKAGHFKDEIVAVEVKGKKGPERFESDEAPRPDTTLEILTKLKPAFRKDG
TITAGNAPGLNSGAAAMIVADRAFAEAKGVTPMAHLVAFGVAAVEPGLFGLGPVPAVRKALERAGWKLSDIERIEINEAF
AAVPLAVAKELGLPQDIINVEGGAVAHGHPIGATGAVLTTRLLHSMKRDGLKRGIVTLCIGGGQGIALAFEAA

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=389, Percent_Identity=46.0154241645244, Blast_Score=367, Evalue=1e-101,
Organism=Homo sapiens, GI167614485, Length=395, Percent_Identity=40.5063291139241, Blast_Score=309, Evalue=3e-84,
Organism=Homo sapiens, GI4557237, Length=394, Percent_Identity=40.1015228426396, Blast_Score=275, Evalue=4e-74,
Organism=Homo sapiens, GI4501853, Length=395, Percent_Identity=38.9873417721519, Blast_Score=234, Evalue=8e-62,
Organism=Homo sapiens, GI4504327, Length=425, Percent_Identity=32.4705882352941, Blast_Score=166, Evalue=5e-41,
Organism=Homo sapiens, GI194353979, Length=390, Percent_Identity=28.974358974359, Blast_Score=111, Evalue=1e-24,
Organism=Escherichia coli, GI1788554, Length=390, Percent_Identity=48.2051282051282, Blast_Score=352, Evalue=2e-98,
Organism=Escherichia coli, GI87082165, Length=389, Percent_Identity=43.9588688946015, Blast_Score=335, Evalue=4e-93,
Organism=Escherichia coli, GI1787663, Length=399, Percent_Identity=44.6115288220551, Blast_Score=301, Evalue=7e-83,
Organism=Escherichia coli, GI48994986, Length=397, Percent_Identity=39.2947103274559, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1788683, Length=426, Percent_Identity=31.6901408450704, Blast_Score=171, Evalue=8e-44,
Organism=Caenorhabditis elegans, GI133906874, Length=393, Percent_Identity=43.0025445292621, Blast_Score=332, Evalue=2e-91,
Organism=Caenorhabditis elegans, GI25147385, Length=384, Percent_Identity=40.1041666666667, Blast_Score=282, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI17535921, Length=391, Percent_Identity=37.5959079283887, Blast_Score=235, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI17535917, Length=391, Percent_Identity=32.2250639386189, Blast_Score=184, Evalue=5e-47,
Organism=Caenorhabditis elegans, GI17551802, Length=424, Percent_Identity=31.6037735849057, Blast_Score=179, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6325229, Length=395, Percent_Identity=38.9873417721519, Blast_Score=256, Evalue=4e-69,
Organism=Saccharomyces cerevisiae, GI6322031, Length=400, Percent_Identity=33.25, Blast_Score=199, Evalue=6e-52,
Organism=Drosophila melanogaster, GI24655093, Length=388, Percent_Identity=44.5876288659794, Blast_Score=346, Evalue=1e-95,
Organism=Drosophila melanogaster, GI17648125, Length=392, Percent_Identity=41.5816326530612, Blast_Score=299, Evalue=2e-81,
Organism=Drosophila melanogaster, GI24640423, Length=389, Percent_Identity=38.8174807197943, Blast_Score=267, Evalue=1e-71,
Organism=Drosophila melanogaster, GI17137578, Length=427, Percent_Identity=32.0843091334895, Blast_Score=159, Evalue=4e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 41243; Mature: 41112

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQRDIVLCSPVRTAIGNYNGSLKNTPATELGAIVVRETLKRAGLESSEIGSVVMGNVIQ
CCCCCEEEECHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
AGNRMNPARQAAIGGGTPVSVPAMTVNRVCGSGAQAIVTAAQEIAIGEIDAAVAGGMENM
CCCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHC
DRAPYLMEGGRWGYRMGPAEILDAMLTDGLNDAFSGEHSGWHTEDLVTQLQITRESQDRF
CCCCEEEECCCCCCCCCHHHHHHHHHHCCCCHHCCCCCCCCCHHHHHHHHHHHHCCHHHH
AVRSQQRFSEAQKAGHFKDEIVAVEVKGKKGPERFESDEAPRPDTTLEILTKLKPAFRKD
HHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHCC
GTITAGNAPGLNSGAAAMIVADRAFAEAKGVTPMAHLVAFGVAAVEPGLFGLGPVPAVRK
CCEECCCCCCCCCCCEEEEEEHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHH
ALERAGWKLSDIERIEINEAFAAVPLAVAKELGLPQDIINVEGGAVAHGHPIGATGAVLT
HHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCHHHHCCCCCEEECCCCCCCHHHHHH
TRLLHSMKRDGLKRGIVTLCIGGGQGIALAFEAA
HHHHHHHHHHHHHCCEEEEEEECCCCEEEEEECC
>Mature Secondary Structure 
TQRDIVLCSPVRTAIGNYNGSLKNTPATELGAIVVRETLKRAGLESSEIGSVVMGNVIQ
CCCCEEEECHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
AGNRMNPARQAAIGGGTPVSVPAMTVNRVCGSGAQAIVTAAQEIAIGEIDAAVAGGMENM
CCCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHC
DRAPYLMEGGRWGYRMGPAEILDAMLTDGLNDAFSGEHSGWHTEDLVTQLQITRESQDRF
CCCCEEEECCCCCCCCCHHHHHHHHHHCCCCHHCCCCCCCCCHHHHHHHHHHHHCCHHHH
AVRSQQRFSEAQKAGHFKDEIVAVEVKGKKGPERFESDEAPRPDTTLEILTKLKPAFRKD
HHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHCC
GTITAGNAPGLNSGAAAMIVADRAFAEAKGVTPMAHLVAFGVAAVEPGLFGLGPVPAVRK
CCEECCCCCCCCCCCEEEEEEHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHH
ALERAGWKLSDIERIEINEAFAAVPLAVAKELGLPQDIINVEGGAVAHGHPIGATGAVLT
HHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCHHHHCCCCCEEECCCCCCCHHHHHH
TRLLHSMKRDGLKRGIVTLCIGGGQGIALAFEAA
HHHHHHHHHHHHHCCEEEEEEECCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]