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The map label for this gene is rlmH [H]

Identifier: 182679844

GI number: 182679844

Start: 3345160

End: 3345672

Strand: Reverse

Name: rlmH [H]

Synonym: Bind_2933

Alternate gene names: 182679844

Gene position: 3345672-3345160 (Counterclockwise)

Preceding gene: 182679845

Following gene: 182679843

Centisome position: 80.23

GC content: 58.28

Gene sequence:

>513_bases
ATGCTGGATTTTTTGTGTGATAGCGAGTGCATGCGTCTCCAGCTCATCGGAATCGGCCGGACCAAAGCCGGGGCTGAACG
GGAGTTGACGGCCCGTTATCTTGAACGGGCGGGGCAAGCTGGACGGGCCATCGGTTTTCCCTCAGTGGAATTGCGGGAAA
TCGAGGAAAGCCGGGCGAGGCGCGGAGACGAACGCAAGGCCCAAGAGGGCAAGGCGATCCGCGCGCTTCTCCAGCCAGCG
AGTCTGATCGTTGCCTTCGATGAACACGGCCGTTCCATCGACAGCGAGGGTTTTGCAGATTGCCTCGGTCAGGCGAGGGA
TTCAGGCAAAGCGATGATGCATTTTCTGATCGGCGGCGCGGATGGATTGGAACCGGCTCTGACCGAGAGTGCCGCTTTTG
TTTTTGCCTTTGGCAAGATGACCTGGCCGCACCAGCTCGTCCGCATCATGGCGGCGGAACAAATTTACCGTGCGATGACG
ATTTTGGGGCATCATCCCTATCATAGAGCATGA

Upstream 100 bases:

>100_bases
GCAACGCCCCCTCTGACGATTGTTGAGGCCCAAGATCTTGGCGGTCCGGCAGGTTCTATCCACGGTCATCTGTATGATTG
TGGTGTGAGAGCCACCGGAC

Downstream 100 bases:

>100_bases
TCGTGCGAGCATGCTTCCATTTGATCATCGTCTATTTGCCAAAATTTAATAGTTTAGGCTGCTGCTTCTCGCAAAAGTTC
GCTTAAATCTGTCTCAGCGT

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH [H]

Number of amino acids: Translated: 170; Mature: 170

Protein sequence:

>170_residues
MLDFLCDSECMRLQLIGIGRTKAGAERELTARYLERAGQAGRAIGFPSVELREIEESRARRGDERKAQEGKAIRALLQPA
SLIVAFDEHGRSIDSEGFADCLGQARDSGKAMMHFLIGGADGLEPALTESAAFVFAFGKMTWPHQLVRIMAAEQIYRAMT
ILGHHPYHRA

Sequences:

>Translated_170_residues
MLDFLCDSECMRLQLIGIGRTKAGAERELTARYLERAGQAGRAIGFPSVELREIEESRARRGDERKAQEGKAIRALLQPA
SLIVAFDEHGRSIDSEGFADCLGQARDSGKAMMHFLIGGADGLEPALTESAAFVFAFGKMTWPHQLVRIMAAEQIYRAMT
ILGHHPYHRA
>Mature_170_residues
MLDFLCDSECMRLQLIGIGRTKAGAERELTARYLERAGQAGRAIGFPSVELREIEESRARRGDERKAQEGKAIRALLQPA
SLIVAFDEHGRSIDSEGFADCLGQARDSGKAMMHFLIGGADGLEPALTESAAFVFAFGKMTWPHQLVRIMAAEQIYRAMT
ILGHHPYHRA

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA [H]

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family [H]

Homologues:

Organism=Escherichia coli, GI1786855, Length=165, Percent_Identity=32.1212121212121, Blast_Score=71, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003742 [H]

Pfam domain/function: PF02590 SPOUT_MTase [H]

EC number: NA

Molecular weight: Translated: 18788; Mature: 18788

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDFLCDSECMRLQLIGIGRTKAGAERELTARYLERAGQAGRAIGFPSVELREIEESRAR
CCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
RGDERKAQEGKAIRALLQPASLIVAFDEHGRSIDSEGFADCLGQARDSGKAMMHFLIGGA
CCCHHHHHHHHHHHHHHCCHHHEEEECCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCC
DGLEPALTESAAFVFAFGKMTWPHQLVRIMAAEQIYRAMTILGHHPYHRA
CCCCHHHHHHHHHEEHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLDFLCDSECMRLQLIGIGRTKAGAERELTARYLERAGQAGRAIGFPSVELREIEESRAR
CCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
RGDERKAQEGKAIRALLQPASLIVAFDEHGRSIDSEGFADCLGQARDSGKAMMHFLIGGA
CCCHHHHHHHHHHHHHHCCHHHEEEECCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCC
DGLEPALTESAAFVFAFGKMTWPHQLVRIMAAEQIYRAMTILGHHPYHRA
CCCCHHHHHHHHHEEHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA