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The map label for this gene is mocB [H]

Identifier: 182679736

GI number: 182679736

Start: 3218246

End: 3219091

Strand: Reverse

Name: mocB [H]

Synonym: Bind_2824

Alternate gene names: 182679736

Gene position: 3219091-3218246 (Counterclockwise)

Preceding gene: 182679737

Following gene: 182679735

Centisome position: 77.19

GC content: 58.04

Gene sequence:

>846_bases
ATGTCATTGTTCGATGACAACTATCTCACCGTTCTGCGCCAGGCGATGGAGAATTATGCAAAGACAAAAGGCGATGTGAC
CGTGCGCTCCGAGGATGCGCAAGGCGACATTGCCCGCCAGCAGAGTCAGATCGATAATTTCATCGCCAGTGGTGTGAATG
CGATCATCGTCATGCTGGTGGACGCCGATTCAAGCGCTGCCATCTCGAGCCAGGCCGAGGCAGCGGGTGTCCCGCTTGTG
TTCGTCAATCAGGCTCCTGTCAATGATCTCCCCACCAAACAGGCGTTTGTCGGCTCCGACGAGGTGTTAGCTGGAAACAT
GCAAGGTCAGGAAGTCTGCAAGGAGTTGAAGGGGACCGGAAAGGTCGTGATCCTCGAAGGTCAGCTCGGCACCACCGGCC
AACGCGGCCGCACTGAGGGCGTGCATCAGGCTCTGTCGGCCCCCGCCTGCAAGAATATCAAGATCGTGGACGAAAAGACC
GCCAATTGGATGCGCACGCCGGCCATGGATCTCGTCACCAATTGGATCTCCGCTGGGCTCGACTTCAATGCCGTCCTTGC
GAACAATGACGAGATGGCGCTGGGCGCTGTTCAGGCGCTCAAAGCCAGCGGCCGTCCAATGAACAGTGTGGTGATCGCCG
GTTCTGACGCGACCAAGGATGCGCTTGCGGCCATGAAGGCCGGCGACCTCGGCGTGACCGTCTTCCAAAATGCCACGGGA
CAAGGCAAGGGCGCGATCGATGCCGCGTTGAAACTCGCCAAGGGCGAAGCGGTGCCGAACAAGACCTACATCCCCTTCGA
GCTCGTGACGAAAGACAATATGGACAAATATCTGAACCGCAATTGA

Upstream 100 bases:

>100_bases
CAAATTAAAAAGGGGAAACGACGTGAAAAGACTTTTGTCCGCGTTAGCGGTGTCTGCGATGACGATAGTCTCCGCGCAGG
CGACAACGATTGGGATCAGT

Downstream 100 bases:

>100_bases
GGTCACGCCAAAGCAGCAATCGTCATCAAGACGTATGGAATTCAGGGAGGGACTCAAATGACCACAGGCCAGCTTCATGC
CGCCGAGAGCCATGCACCGC

Product: periplasmic binding protein/LacI transcriptional regulator

Products: ADP; phosphate; beta-D-galactose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MSLFDDNYLTVLRQAMENYAKTKGDVTVRSEDAQGDIARQQSQIDNFIASGVNAIIVMLVDADSSAAISSQAEAAGVPLV
FVNQAPVNDLPTKQAFVGSDEVLAGNMQGQEVCKELKGTGKVVILEGQLGTTGQRGRTEGVHQALSAPACKNIKIVDEKT
ANWMRTPAMDLVTNWISAGLDFNAVLANNDEMALGAVQALKASGRPMNSVVIAGSDATKDALAAMKAGDLGVTVFQNATG
QGKGAIDAALKLAKGEAVPNKTYIPFELVTKDNMDKYLNRN

Sequences:

>Translated_281_residues
MSLFDDNYLTVLRQAMENYAKTKGDVTVRSEDAQGDIARQQSQIDNFIASGVNAIIVMLVDADSSAAISSQAEAAGVPLV
FVNQAPVNDLPTKQAFVGSDEVLAGNMQGQEVCKELKGTGKVVILEGQLGTTGQRGRTEGVHQALSAPACKNIKIVDEKT
ANWMRTPAMDLVTNWISAGLDFNAVLANNDEMALGAVQALKASGRPMNSVVIAGSDATKDALAAMKAGDLGVTVFQNATG
QGKGAIDAALKLAKGEAVPNKTYIPFELVTKDNMDKYLNRN
>Mature_280_residues
SLFDDNYLTVLRQAMENYAKTKGDVTVRSEDAQGDIARQQSQIDNFIASGVNAIIVMLVDADSSAAISSQAEAAGVPLVF
VNQAPVNDLPTKQAFVGSDEVLAGNMQGQEVCKELKGTGKVVILEGQLGTTGQRGRTEGVHQALSAPACKNIKIVDEKTA
NWMRTPAMDLVTNWISAGLDFNAVLANNDEMALGAVQALKASGRPMNSVVIAGSDATKDALAAMKAGDLGVTVFQNATGQ
GKGAIDAALKLAKGEAVPNKTYIPFELVTKDNMDKYLNRN

Specific function: Involved in rhizopine (L-3-O-methyl-scyllo-inosamine) catabolism. Could be involved in its high affinity transport [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788473, Length=299, Percent_Identity=33.7792642140468, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1790192, Length=241, Percent_Identity=29.4605809128631, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1790674, Length=283, Percent_Identity=28.6219081272085, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790526, Length=266, Percent_Identity=25.5639097744361, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1786540, Length=232, Percent_Identity=26.7241379310345, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: 4720 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1320 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 29648; Mature: 29517

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLFDDNYLTVLRQAMENYAKTKGDVTVRSEDAQGDIARQQSQIDNFIASGVNAIIVMLV
CCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEE
DADSSAAISSQAEAAGVPLVFVNQAPVNDLPTKQAFVGSDEVLAGNMQGQEVCKELKGTG
CCCCCCHHCCCHHHCCCCEEEECCCCCCCCCCCHHCCCCCCEEECCCCHHHHHHHHCCCC
KVVILEGQLGTTGQRGRTEGVHQALSAPACKNIKIVDEKTANWMRTPAMDLVTNWISAGL
CEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCCCHHCCCHHHHHHHHHHCCC
DFNAVLANNDEMALGAVQALKASGRPMNSVVIAGSDATKDALAAMKAGDLGVTVFQNATG
CEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHCCCCCEEEEECCCC
QGKGAIDAALKLAKGEAVPNKTYIPFELVTKDNMDKYLNRN
CCCCHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHCCC
>Mature Secondary Structure 
SLFDDNYLTVLRQAMENYAKTKGDVTVRSEDAQGDIARQQSQIDNFIASGVNAIIVMLV
CCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEE
DADSSAAISSQAEAAGVPLVFVNQAPVNDLPTKQAFVGSDEVLAGNMQGQEVCKELKGTG
CCCCCCHHCCCHHHCCCCEEEECCCCCCCCCCCHHCCCCCCEEECCCCHHHHHHHHCCCC
KVVILEGQLGTTGQRGRTEGVHQALSAPACKNIKIVDEKTANWMRTPAMDLVTNWISAGL
CEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCCCHHCCCHHHHHHHHHHCCC
DFNAVLANNDEMALGAVQALKASGRPMNSVVIAGSDATKDALAAMKAGDLGVTVFQNATG
CEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHCCCCCEEEEECCCC
QGKGAIDAALKLAKGEAVPNKTYIPFELVTKDNMDKYLNRN
CCCCHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; beta-D-galactose [Periplasm]; H2O [C]

Specific reaction: ATP + beta-D-galactose [Periplasm] + H2O = ADP + phosphate + beta-D-galactose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7845353 [H]