Click here to switch to the map view.

The map label for this gene is pcm [H]

Identifier: 182679615

GI number: 182679615

Start: 3078652

End: 3079344

Strand: Reverse

Name: pcm [H]

Synonym: Bind_2700

Alternate gene names: 182679615

Gene position: 3079344-3078652 (Counterclockwise)

Preceding gene: 182679616

Following gene: 182679614

Centisome position: 73.84

GC content: 62.05

Gene sequence:

>693_bases
ATGACGGCAGCAGAGTCTCCCCGGCCTCTTTCTCGCGATGCGTTGCCTATTGCAGAGGCGGAGACCAAAGCCGCATTCCT
GCTACGGCTGCGCAGTGGCGGGATCCGCGATCTCGCGCTCCTGCGGGCGCTCGAGATCGTGCCGCGCGAGATTTTCGTGC
CGCAACAGTTTGCGGATTTCGCGGGGCGTGATCTCGCCTTGCCGCTCCCTTGCGGCCAGACCATGACCGCGCCCTCGCTG
CTCGCCCGGATGATCGCTGCTCTCGATCTTTCCCCCCGTCATCGCGTGCTCGAGATCGGGACCGGCTCCGGTTATGCGAC
GGCCGTGCTCTCGCATATCGTGCGGGAAGTCGTTTCCTTCGAGCGTTTCCACACGCTCGCCGAGGCTGCACGGATGCGGC
TTGCAGCGCTTGCGCGTGATAATATCCTGGCTTTCTGGGGCGATGGTCTCGCTGTGCCAGTGGATATTGGCTCTTTCGAC
CGGATCATCGTTCACGCGGCCAGCCGCGACATTCCTGCCGGATTACTCGAACGGCTCGCTGATGATGGCCTGCTCCTTTT
TGCGCGAGAGATAGCGGACGAAGGTCAAAGCCTGTGGCGAATCGCTCGCGGGCGAGAGTCGAGCCGTTTGGAAGAAGATT
ACGGTCCTTGCCATTTCCAGCCGTTGCTTCCGGGTGTGGCTCGCTTTCTTTAA

Upstream 100 bases:

>100_bases
GCTGACGCCGCTGCGCATCGATCTGACCGATGATCCGACCATGACTCGACTGGCACAAAGCCTGCCCAAAACCTTGCCCA
AAGTTGCCGGTTAGCCTCGC

Downstream 100 bases:

>100_bases
TTTGCCGAACGCCGAGATTCGTGCCTAAAGGGTTCTCGCCCACCCGACATGCGTGGATTGGCCCCGCAGGGCGCCGTGGG
ACGCTGCAAAGCTTCGCCTG

Product: protein-L-isoaspartate(D-aspartate) O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT [H]

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MTAAESPRPLSRDALPIAEAETKAAFLLRLRSGGIRDLALLRALEIVPREIFVPQQFADFAGRDLALPLPCGQTMTAPSL
LARMIAALDLSPRHRVLEIGTGSGYATAVLSHIVREVVSFERFHTLAEAARMRLAALARDNILAFWGDGLAVPVDIGSFD
RIIVHAASRDIPAGLLERLADDGLLLFAREIADEGQSLWRIARGRESSRLEEDYGPCHFQPLLPGVARFL

Sequences:

>Translated_230_residues
MTAAESPRPLSRDALPIAEAETKAAFLLRLRSGGIRDLALLRALEIVPREIFVPQQFADFAGRDLALPLPCGQTMTAPSL
LARMIAALDLSPRHRVLEIGTGSGYATAVLSHIVREVVSFERFHTLAEAARMRLAALARDNILAFWGDGLAVPVDIGSFD
RIIVHAASRDIPAGLLERLADDGLLLFAREIADEGQSLWRIARGRESSRLEEDYGPCHFQPLLPGVARFL
>Mature_229_residues
TAAESPRPLSRDALPIAEAETKAAFLLRLRSGGIRDLALLRALEIVPREIFVPQQFADFAGRDLALPLPCGQTMTAPSLL
ARMIAALDLSPRHRVLEIGTGSGYATAVLSHIVREVVSFERFHTLAEAARMRLAALARDNILAFWGDGLAVPVDIGSFDR
IIVHAASRDIPAGLLERLADDGLLLFAREIADEGQSLWRIARGRESSRLEEDYGPCHFQPLLPGVARFL

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789100, Length=201, Percent_Identity=41.7910447761194, Blast_Score=134, Evalue=4e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 25139; Mature: 25008

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAAESPRPLSRDALPIAEAETKAAFLLRLRSGGIRDLALLRALEIVPREIFVPQQFADF
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHH
AGRDLALPLPCGQTMTAPSLLARMIAALDLSPRHRVLEIGTGSGYATAVLSHIVREVVSF
CCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHH
ERFHTLAEAARMRLAALARDNILAFWGDGLAVPVDIGSFDRIIVHAASRDIPAGLLERLA
HHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEEECCCCCHHEEECCCCCCHHHHHHHHC
DDGLLLFAREIADEGQSLWRIARGRESSRLEEDYGPCHFQPLLPGVARFL
CCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHC
>Mature Secondary Structure 
TAAESPRPLSRDALPIAEAETKAAFLLRLRSGGIRDLALLRALEIVPREIFVPQQFADF
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHH
AGRDLALPLPCGQTMTAPSLLARMIAALDLSPRHRVLEIGTGSGYATAVLSHIVREVVSF
CCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHH
ERFHTLAEAARMRLAALARDNILAFWGDGLAVPVDIGSFDRIIVHAASRDIPAGLLERLA
HHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEEECCCCCHHEEECCCCCCHHHHHHHHC
DDGLLLFAREIADEGQSLWRIARGRESSRLEEDYGPCHFQPLLPGVARFL
CCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA