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The map label for this gene is cobS [H]

Identifier: 182679612

GI number: 182679612

Start: 3075389

End: 3076216

Strand: Reverse

Name: cobS [H]

Synonym: Bind_2697

Alternate gene names: 182679612

Gene position: 3076216-3075389 (Counterclockwise)

Preceding gene: 182679613

Following gene: 182679605

Centisome position: 73.77

GC content: 59.9

Gene sequence:

>828_bases
ATGGATTTGACCTCAAAGGAAACCATGCATTTTGTCGACGCCCCCTTTTTAAGAGACCTCTCTTTCTGCCTGCGGTTTTA
TACACGCTTGCCGATTCCCGCTTTGGGCGGCGAAACCGATCCGCATGGCACCGAGAATGGATTTGAGCGGCTGACGCGGG
CGGTTCGTGTTCTGCCGCTTGCTGGCGTTCTCATCGGTGGTTTTGGCGCTGCCGTGCTGTTGCTCACATCCTGGATCGGC
TTTGGTCCCTGGCTCGCCGGGCCCTTGTCAATTCTCGCACTCATCCTGGCGACCGGGGGGTTGCATGAGGATGGGCTCGC
CGATTGCGCGGATGGGTTTGCGGGTGGTTTTTCCGTCGCGCGCAAATTGGAGATCATGCGTGACAGCCGGCTTGGAACCT
ATGGCGTTCTGGCCCTGATCCTGGCTCTGTGGTTGCGCATTGCTGGTCTTGCGACGATCGGCGAAGGGAGCCTTGTTCTT
GCCGCCTTTGTGCTCGTGGCGGCTGCGGCCCTCTCCCGCACAATGGCGCTTTTGCCCTTGTCGATCTTGCCTCCGGCGCG
GGCGGATGGTGCGGGTTTCAATGCCGGGCGGCCAAGTCCGGTGATCGTGATTCAGGCTTTTGGTCTTGCCGCCGCCGTTG
GTTTGCTGCCACTCGCGGCGGGTGCTTCTCTCTTGAGGGTCCTATTCGCCTTGGCGATTGCGCTGGCAGCAGCCTATGGC
CTCACTCGTCTGGCCTATACACAGATTCGAGGGCATACTGGCGATGTGGCCGGGGCGGTCCAACAAATTGTGGAAATTGT
CTATTTGCTCTGCTTCGCGGCGGGATAG

Upstream 100 bases:

>100_bases
GCGTCGAGGATCTCGAGGAATATGGCGAGGCCTGAAATCGGGGAGGGCCTGAAATCGCGAAGGGGAAGGAGAGTCGTTTC
GTAAGGCCCTCGACAAGATC

Downstream 100 bases:

>100_bases
CTGGGCAGCATCGATCCGAAAAGCGAAGACCACTTTTCGGATCGATCTGATGCGTTCGACTCAGCCTTACAATATCGCTC
CTGCGTATGATTTATTCCGC

Product: cobalamin 5'-phosphate synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MDLTSKETMHFVDAPFLRDLSFCLRFYTRLPIPALGGETDPHGTENGFERLTRAVRVLPLAGVLIGGFGAAVLLLTSWIG
FGPWLAGPLSILALILATGGLHEDGLADCADGFAGGFSVARKLEIMRDSRLGTYGVLALILALWLRIAGLATIGEGSLVL
AAFVLVAAAALSRTMALLPLSILPPARADGAGFNAGRPSPVIVIQAFGLAAAVGLLPLAAGASLLRVLFALAIALAAAYG
LTRLAYTQIRGHTGDVAGAVQQIVEIVYLLCFAAG

Sequences:

>Translated_275_residues
MDLTSKETMHFVDAPFLRDLSFCLRFYTRLPIPALGGETDPHGTENGFERLTRAVRVLPLAGVLIGGFGAAVLLLTSWIG
FGPWLAGPLSILALILATGGLHEDGLADCADGFAGGFSVARKLEIMRDSRLGTYGVLALILALWLRIAGLATIGEGSLVL
AAFVLVAAAALSRTMALLPLSILPPARADGAGFNAGRPSPVIVIQAFGLAAAVGLLPLAAGASLLRVLFALAIALAAAYG
LTRLAYTQIRGHTGDVAGAVQQIVEIVYLLCFAAG
>Mature_275_residues
MDLTSKETMHFVDAPFLRDLSFCLRFYTRLPIPALGGETDPHGTENGFERLTRAVRVLPLAGVLIGGFGAAVLLLTSWIG
FGPWLAGPLSILALILATGGLHEDGLADCADGFAGGFSVARKLEIMRDSRLGTYGVLALILALWLRIAGLATIGEGSLVL
AAFVLVAAAALSRTMALLPLSILPPARADGAGFNAGRPSPVIVIQAFGLAAAVGLLPLAAGASLLRVLFALAIALAAAYG
LTRLAYTQIRGHTGDVAGAVQQIVEIVYLLCFAAG

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=252, Percent_Identity=38.4920634920635, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 28444; Mature: 28444

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLTSKETMHFVDAPFLRDLSFCLRFYTRLPIPALGGETDPHGTENGFERLTRAVRVLPL
CCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
AGVLIGGFGAAVLLLTSWIGFGPWLAGPLSILALILATGGLHEDGLADCADGFAGGFSVA
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
RKLEIMRDSRLGTYGVLALILALWLRIAGLATIGEGSLVLAAFVLVAAAALSRTMALLPL
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SILPPARADGAGFNAGRPSPVIVIQAFGLAAAVGLLPLAAGASLLRVLFALAIALAAAYG
HHCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTRLAYTQIRGHTGDVAGAVQQIVEIVYLLCFAAG
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDLTSKETMHFVDAPFLRDLSFCLRFYTRLPIPALGGETDPHGTENGFERLTRAVRVLPL
CCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
AGVLIGGFGAAVLLLTSWIGFGPWLAGPLSILALILATGGLHEDGLADCADGFAGGFSVA
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
RKLEIMRDSRLGTYGVLALILALWLRIAGLATIGEGSLVLAAFVLVAAAALSRTMALLPL
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SILPPARADGAGFNAGRPSPVIVIQAFGLAAAVGLLPLAAGASLLRVLFALAIALAAAYG
HHCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTRLAYTQIRGHTGDVAGAVQQIVEIVYLLCFAAG
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]