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The map label for this gene is rffG [C]

Identifier: 182679576

GI number: 182679576

Start: 3024202

End: 3025200

Strand: Reverse

Name: rffG [C]

Synonym: Bind_2656

Alternate gene names: 182679576

Gene position: 3025200-3024202 (Counterclockwise)

Preceding gene: 182679579

Following gene: 182679574

Centisome position: 72.54

GC content: 51.25

Gene sequence:

>999_bases
ATGGATCCCATAATAGTCACAGGTGCAGCTGGTTTTATAGGCATGCATGTGGCTGAAAGGCTGTTGGATCGGGGAGAGGC
TGTCGTTGGTATCGACATATTCAATTCCTATTACGACCCAACCCTAAAAGCCGCGCGAGCTGCACGTTTAGAAAGTCGAC
CAAGCTTTTCGATGGTCAGGATGGATATTGCCGACCACGAGAGCTTCCTAGCTCTCGTTAAGCGGTCCGGTGTCCGCCGT
ATCGTTCACCTGGCCGCCCAAGCTGGGGTGCGCTATTCGATTGATAATCCATTCGCCTACGAGCATTCGAATCTAGCCGG
GCACCTCTCTGTGCTGGAAGCATGTCGCCATGCCAAGATCGAACACCTCGTCTATGCCTCGTCGAGTTCCGTCTATGGTG
ATCGGCCGCTTACCGCTTCCGGCTTCAAGGAGACAGATCCGGTTGATGCACCCGTTTCGCTTTATGCAGCGACCAAGCGC
TCATGCGAACTAATCAGCCAAAGCTACGCCCGGCTTTACGGTTTTCCGCAATCGGGTCTGCGTTTCTTTACCGTCTATGG
TCCCTGGGGGCGGCCAGATATGGCTTATTACGGATTCACCGAGAAAATTCTCGCAGGCCACCCCATCGAAGTCTATGGCG
ACGGTAAAATGTCCCGTGATTTCACTTTTATCGACGATATCGTAGATGGGATCATTGGCATTCTTGATTGTCCGCCGCTC
ATGGGGGATCATGAGATCTATAACATTGGCGACAGTCAACCAGTTAGCCTGATGGACATGATCGCTACTTTGGAACTGGC
TCTTGGTCGTGAAGCGGTTAAGATTTTCCGTCCTATGCAGCCGGGCGACGTGACCGCCACTTATGCAGATGTTTCCAAAC
TATATAGTTTGATAGGCTACCAACCAAAAGTTAACCTAGCAACGGGACTACAACGTTTTGTTCACTGGCGCTTGAGTTTT
GATGCGTCGTCTACGAAAATCAAAGCCCCAGTAGTTTGA

Upstream 100 bases:

>100_bases
TCGTTCCTTCAACGGAGAAGTGGAGGCAAGATTATCTTTTATGATACGTGATTGTTCCTCTACTCATCACCAACTTCCGC
TTATAGTTTAGGAGGCAAAC

Downstream 100 bases:

>100_bases
CAACAGAAATTTCTGTGCTGTTACTTCACTTCGTAAGTGTGGCTTTTCCTACGTATATATTTTTCGTATACATTTTTAAA
AGACCGCGGCTTTTGAGGCA

Product: NAD-dependent epimerase/dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MDPIIVTGAAGFIGMHVAERLLDRGEAVVGIDIFNSYYDPTLKAARAARLESRPSFSMVRMDIADHESFLALVKRSGVRR
IVHLAAQAGVRYSIDNPFAYEHSNLAGHLSVLEACRHAKIEHLVYASSSSVYGDRPLTASGFKETDPVDAPVSLYAATKR
SCELISQSYARLYGFPQSGLRFFTVYGPWGRPDMAYYGFTEKILAGHPIEVYGDGKMSRDFTFIDDIVDGIIGILDCPPL
MGDHEIYNIGDSQPVSLMDMIATLELALGREAVKIFRPMQPGDVTATYADVSKLYSLIGYQPKVNLATGLQRFVHWRLSF
DASSTKIKAPVV

Sequences:

>Translated_332_residues
MDPIIVTGAAGFIGMHVAERLLDRGEAVVGIDIFNSYYDPTLKAARAARLESRPSFSMVRMDIADHESFLALVKRSGVRR
IVHLAAQAGVRYSIDNPFAYEHSNLAGHLSVLEACRHAKIEHLVYASSSSVYGDRPLTASGFKETDPVDAPVSLYAATKR
SCELISQSYARLYGFPQSGLRFFTVYGPWGRPDMAYYGFTEKILAGHPIEVYGDGKMSRDFTFIDDIVDGIIGILDCPPL
MGDHEIYNIGDSQPVSLMDMIATLELALGREAVKIFRPMQPGDVTATYADVSKLYSLIGYQPKVNLATGLQRFVHWRLSF
DASSTKIKAPVV
>Mature_332_residues
MDPIIVTGAAGFIGMHVAERLLDRGEAVVGIDIFNSYYDPTLKAARAARLESRPSFSMVRMDIADHESFLALVKRSGVRR
IVHLAAQAGVRYSIDNPFAYEHSNLAGHLSVLEACRHAKIEHLVYASSSSVYGDRPLTASGFKETDPVDAPVSLYAATKR
SCELISQSYARLYGFPQSGLRFFTVYGPWGRPDMAYYGFTEKILAGHPIEVYGDGKMSRDFTFIDDIVDGIIGILDCPPL
MGDHEIYNIGDSQPVSLMDMIATLELALGREAVKIFRPMQPGDVTATYADVSKLYSLIGYQPKVNLATGLQRFVHWRLSF
DASSTKIKAPVV

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=328, Percent_Identity=24.390243902439, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI42516563, Length=337, Percent_Identity=24.3323442136499, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI56237023, Length=340, Percent_Identity=25.5882352941176, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI56118217, Length=340, Percent_Identity=25.5882352941176, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI189083684, Length=340, Percent_Identity=25.5882352941176, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI193211614, Length=283, Percent_Identity=25.4416961130742, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI8393516, Length=283, Percent_Identity=25.4416961130742, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI48994969, Length=351, Percent_Identity=28.4900284900285, Blast_Score=117, Evalue=8e-28,
Organism=Escherichia coli, GI1786974, Length=335, Percent_Identity=25.6716417910448, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788353, Length=358, Percent_Identity=25.6983240223464, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1788589, Length=281, Percent_Identity=24.9110320284698, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71982035, Length=347, Percent_Identity=27.9538904899135, Blast_Score=104, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI71982038, Length=349, Percent_Identity=27.5071633237822, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17568069, Length=341, Percent_Identity=24.633431085044, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17539532, Length=329, Percent_Identity=26.7477203647416, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI115532424, Length=329, Percent_Identity=26.1398176291793, Blast_Score=91, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6319493, Length=348, Percent_Identity=22.9885057471264, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21356223, Length=325, Percent_Identity=27.6923076923077, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI19923002, Length=340, Percent_Identity=25.8823529411765, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 36597; Mature: 36597

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPIIVTGAAGFIGMHVAERLLDRGEAVVGIDIFNSYYDPTLKAARAARLESRPSFSMVR
CCCEEEECCCHHHHHHHHHHHHHCCCEEEEEEHHHHHCCHHHHHHHHHHHCCCCCCEEEE
MDIADHESFLALVKRSGVRRIVHLAAQAGVRYSIDNPFAYEHSNLAGHLSVLEACRHAKI
EEHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEECCCCCHHHHHHHHHHHHCCH
EHLVYASSSSVYGDRPLTASGFKETDPVDAPVSLYAATKRSCELISQSYARLYGFPQSGL
HHEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RFFTVYGPWGRPDMAYYGFTEKILAGHPIEVYGDGKMSRDFTFIDDIVDGIIGILDCPPL
EEEEEECCCCCCCCEECCCHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCC
MGDHEIYNIGDSQPVSLMDMIATLELALGREAVKIFRPMQPGDVTATYADVSKLYSLIGY
CCCCEEEECCCCCCHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCEEHHHHHHHHHHHHCC
QPKVNLATGLQRFVHWRLSFDASSTKIKAPVV
CCCCCHHHHHHHHHEEEEEECCCCCEEECCCC
>Mature Secondary Structure
MDPIIVTGAAGFIGMHVAERLLDRGEAVVGIDIFNSYYDPTLKAARAARLESRPSFSMVR
CCCEEEECCCHHHHHHHHHHHHHCCCEEEEEEHHHHHCCHHHHHHHHHHHCCCCCCEEEE
MDIADHESFLALVKRSGVRRIVHLAAQAGVRYSIDNPFAYEHSNLAGHLSVLEACRHAKI
EEHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEECCCCCHHHHHHHHHHHHCCH
EHLVYASSSSVYGDRPLTASGFKETDPVDAPVSLYAATKRSCELISQSYARLYGFPQSGL
HHEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RFFTVYGPWGRPDMAYYGFTEKILAGHPIEVYGDGKMSRDFTFIDDIVDGIIGILDCPPL
EEEEEECCCCCCCCEECCCHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCC
MGDHEIYNIGDSQPVSLMDMIATLELALGREAVKIFRPMQPGDVTATYADVSKLYSLIGY
CCCCEEEECCCCCCHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCEEHHHHHHHHHHHHCC
QPKVNLATGLQRFVHWRLSFDASSTKIKAPVV
CCCCCHHHHHHHHHEEEEEECCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7682279 [H]