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The map label for this gene is menE [H]

Identifier: 182679525

GI number: 182679525

Start: 2952807

End: 2954285

Strand: Reverse

Name: menE [H]

Synonym: Bind_2587

Alternate gene names: 182679525

Gene position: 2954285-2952807 (Counterclockwise)

Preceding gene: 182679526

Following gene: 182679524

Centisome position: 70.84

GC content: 55.51

Gene sequence:

>1479_bases
ATGCGCACGATCTTTGACGCAATGAGGAGCCACGCGCAAAGCGCCGGCGATGTCAAATCGATAAGTGATCAAACCGAGAC
GCTTACTCGCCGAGACTTATTGGCACGGGTGGCGGCGCTAGCTTCGGATCTCCGCAGGTTACCACGTGTAATCGGTCTGT
TTGCGCCCAATGGCGTAGAATGGGTCATTGGCCAGCTTGCCTGTGTCCTCGCGGGCAAGATCGCTGTGCCCTTACCGACT
TTCTTCAGTGATAACCAACTTGCCTATATCATCCGTAATGCCTCGATCGAACTCATTCTGGTGGGCCAAGCAACTTTATC
GCGCGCGCTGCAATCGGGTGTCGATATTCACCTCATTGACAACCATCGCTATGAGTGGCAGCCGGCCGATATTGTCGATG
GTTTTGGTCAAGTGATCTACACGTCCGGGAGCACAGGGAATCCAAAAGGTGTACGTCACGAAAGCGGGCAGATCGCATGG
TCGGCGGCCGCGCTTGCAGCGGCGACTGACGCGCATGAAAGCGACCGTTACCTTTCCGTCCTTCCTTTGCCTCTTCTTCT
GGAGACGATCTGCGCCGTCTTTGTTCCTCAACTAATCGGCGCATATGCTTATTTTGACAGTCGAGCGGCTGAGGCTGTTG
GACGAAGCAATCCAACCGAACTTGCTGCTGCCTTCGAGAGACATCGACCAACGACGAGTGTTGTCGTTCCTCAATTGCTC
AGGTCTTGGACAGGCGAGCTCTTGGCCACTGGCGGACGAGCACCCGCTTCACTGCGGTTCGTAGCGGTCGGTGGTGCTCC
TGTGCCAGCGGCCCTCGTGACGGCTGCGTGGGATCTCGGGATCCCGGTACACGAAGGCTATGGCCTTTCCGAGTGTTGTT
CAGTCGTGGCGGTCAATTGCGCGAGTGAGCGGCGCGCGGGCACAGTGGGACGACCGCTGAGAGGTTTGAAGGTCTCGATC
GAGGAAGGAGAGATCGTCGTCGATGGTCCTTCGGTAATGGACGGCTATCTCGCTCAAGCGGCCGCGCGACGCCCCTGGCG
AACTGGCGACCTGGGGAAGTTGGATGTCGACGGCTTTCTCATCGTGCTAGGGCGCAAGGACAATCTCATTGTCACAAGCT
TTGGCCGCAACATTAGCCCCGAATGGATAGAAACGATGCTTCTCGGCGATCCGCGTATTGCGTTTTGCGCCGTCATCGGT
AACGGCGAGTCATATCTTACTGCTATCCTCATCCCCTCTGCCCACGGCTCCGCTTGGTTTGCCTCTGCAAGTGAAAAGAA
TGTGCACGACATGATCGCAGAATGTTGTGCAAATGCCCCAGATTATGCGGTGCCAAGCGCATTCATTCTTGTCTCCTTAG
ATCAAGCGAGAGACGCGCAGATTCTCACTAGTAACTTTCGGTTTGTACGCAAAACGTTGGCCTCATTCGTCACAAATAGA
TCGTTGCATATGACCGATCGCTCGAAGAATGTTTCTTAA

Upstream 100 bases:

>100_bases
GAGCGTTGGGGCAAATATTATACCACAGAACCCAAGGTATATGCGGTGGAAAGCACTCGATTCCATACGTACCGTGGTTC
CGACAAAGACAAACAGACCC

Downstream 100 bases:

>100_bases
CTAACTCGAAAGTTCTGACAATGACCTTCTACGAACGTCTCATTTCTGAAACTACCGACGAGCGGGACGCTTTCCTCGAA
ATCCCACTTATCCAGCGCAC

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MRTIFDAMRSHAQSAGDVKSISDQTETLTRRDLLARVAALASDLRRLPRVIGLFAPNGVEWVIGQLACVLAGKIAVPLPT
FFSDNQLAYIIRNASIELILVGQATLSRALQSGVDIHLIDNHRYEWQPADIVDGFGQVIYTSGSTGNPKGVRHESGQIAW
SAAALAAATDAHESDRYLSVLPLPLLLETICAVFVPQLIGAYAYFDSRAAEAVGRSNPTELAAAFERHRPTTSVVVPQLL
RSWTGELLATGGRAPASLRFVAVGGAPVPAALVTAAWDLGIPVHEGYGLSECCSVVAVNCASERRAGTVGRPLRGLKVSI
EEGEIVVDGPSVMDGYLAQAAARRPWRTGDLGKLDVDGFLIVLGRKDNLIVTSFGRNISPEWIETMLLGDPRIAFCAVIG
NGESYLTAILIPSAHGSAWFASASEKNVHDMIAECCANAPDYAVPSAFILVSLDQARDAQILTSNFRFVRKTLASFVTNR
SLHMTDRSKNVS

Sequences:

>Translated_492_residues
MRTIFDAMRSHAQSAGDVKSISDQTETLTRRDLLARVAALASDLRRLPRVIGLFAPNGVEWVIGQLACVLAGKIAVPLPT
FFSDNQLAYIIRNASIELILVGQATLSRALQSGVDIHLIDNHRYEWQPADIVDGFGQVIYTSGSTGNPKGVRHESGQIAW
SAAALAAATDAHESDRYLSVLPLPLLLETICAVFVPQLIGAYAYFDSRAAEAVGRSNPTELAAAFERHRPTTSVVVPQLL
RSWTGELLATGGRAPASLRFVAVGGAPVPAALVTAAWDLGIPVHEGYGLSECCSVVAVNCASERRAGTVGRPLRGLKVSI
EEGEIVVDGPSVMDGYLAQAAARRPWRTGDLGKLDVDGFLIVLGRKDNLIVTSFGRNISPEWIETMLLGDPRIAFCAVIG
NGESYLTAILIPSAHGSAWFASASEKNVHDMIAECCANAPDYAVPSAFILVSLDQARDAQILTSNFRFVRKTLASFVTNR
SLHMTDRSKNVS
>Mature_492_residues
MRTIFDAMRSHAQSAGDVKSISDQTETLTRRDLLARVAALASDLRRLPRVIGLFAPNGVEWVIGQLACVLAGKIAVPLPT
FFSDNQLAYIIRNASIELILVGQATLSRALQSGVDIHLIDNHRYEWQPADIVDGFGQVIYTSGSTGNPKGVRHESGQIAW
SAAALAAATDAHESDRYLSVLPLPLLLETICAVFVPQLIGAYAYFDSRAAEAVGRSNPTELAAAFERHRPTTSVVVPQLL
RSWTGELLATGGRAPASLRFVAVGGAPVPAALVTAAWDLGIPVHEGYGLSECCSVVAVNCASERRAGTVGRPLRGLKVSI
EEGEIVVDGPSVMDGYLAQAAARRPWRTGDLGKLDVDGFLIVLGRKDNLIVTSFGRNISPEWIETMLLGDPRIAFCAVIG
NGESYLTAILIPSAHGSAWFASASEKNVHDMIAECCANAPDYAVPSAFILVSLDQARDAQILTSNFRFVRKTLASFVTNR
SLHMTDRSKNVS

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG1022

COG function: function code I; Long-chain acyl-CoA synthetases (AMP-forming)

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI40807491, Length=496, Percent_Identity=24.7983870967742, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI57165412, Length=494, Percent_Identity=25.5060728744939, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI57165410, Length=494, Percent_Identity=25.5060728744939, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI27477105, Length=473, Percent_Identity=25.369978858351, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1789201, Length=288, Percent_Identity=27.7777777777778, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788595, Length=405, Percent_Identity=28.3950617283951, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI145693145, Length=423, Percent_Identity=24.3498817966903, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1788107, Length=165, Percent_Identity=32.1212121212121, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1790505, Length=479, Percent_Identity=22.5469728601253, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI25147511, Length=517, Percent_Identity=25.7253384912959, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17558820, Length=431, Percent_Identity=24.1299303944316, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17553312, Length=524, Percent_Identity=23.2824427480916, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17510401, Length=248, Percent_Identity=25, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19921316, Length=341, Percent_Identity=24.633431085044, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 52772; Mature: 52772

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTIFDAMRSHAQSAGDVKSISDQTETLTRRDLLARVAALASDLRRLPRVIGLFAPNGVE
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHH
WVIGQLACVLAGKIAVPLPTFFSDNQLAYIIRNASIELILVGQATLSRALQSGVDIHLID
HHHHHHHHHHHCCCEECCCCCCCCCEEEEEEECCCEEEEEECHHHHHHHHHCCCEEEEEE
NHRYEWQPADIVDGFGQVIYTSGSTGNPKGVRHESGQIAWSAAALAAATDAHESDRYLSV
CCCCCCCCHHHHCCCCCEEEECCCCCCCCCCCCCCCCEEEHHHHHHHHCCCCCCCCEEEE
LPLPLLLETICAVFVPQLIGAYAYFDSRAAEAVGRSNPTELAAAFERHRPTTSVVVPQLL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHH
RSWTGELLATGGRAPASLRFVAVGGAPVPAALVTAAWDLGIPVHEGYGLSECCSVVAVNC
HHHCCHHEECCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCC
ASERRAGTVGRPLRGLKVSIEEGEIVVDGPSVMDGYLAQAAARRPWRTGDLGKLDVDGFL
CHHCCCCCCCCCCCCEEEEEECCEEEECCCHHHHHHHHHHHHCCCCCCCCCCEEECCCEE
IVLGRKDNLIVTSFGRNISPEWIETMLLGDPRIAFCAVIGNGESYLTAILIPSAHGSAWF
EEEECCCCEEEEECCCCCCHHHHHHHHCCCCCEEEEEEEECCCCEEEEEEEECCCCCEEE
ASASEKNVHDMIAECCANAPDYAVPSAFILVSLDQARDAQILTSNFRFVRKTLASFVTNR
CCCCCCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCC
SLHMTDRSKNVS
CEEECCCCCCCC
>Mature Secondary Structure
MRTIFDAMRSHAQSAGDVKSISDQTETLTRRDLLARVAALASDLRRLPRVIGLFAPNGVE
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHH
WVIGQLACVLAGKIAVPLPTFFSDNQLAYIIRNASIELILVGQATLSRALQSGVDIHLID
HHHHHHHHHHHCCCEECCCCCCCCCEEEEEEECCCEEEEEECHHHHHHHHHCCCEEEEEE
NHRYEWQPADIVDGFGQVIYTSGSTGNPKGVRHESGQIAWSAAALAAATDAHESDRYLSV
CCCCCCCCHHHHCCCCCEEEECCCCCCCCCCCCCCCCEEEHHHHHHHHCCCCCCCCEEEE
LPLPLLLETICAVFVPQLIGAYAYFDSRAAEAVGRSNPTELAAAFERHRPTTSVVVPQLL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHH
RSWTGELLATGGRAPASLRFVAVGGAPVPAALVTAAWDLGIPVHEGYGLSECCSVVAVNC
HHHCCHHEECCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCC
ASERRAGTVGRPLRGLKVSIEEGEIVVDGPSVMDGYLAQAAARRPWRTGDLGKLDVDGFL
CHHCCCCCCCCCCCCEEEEEECCEEEECCCHHHHHHHHHHHHCCCCCCCCCCEEECCCEE
IVLGRKDNLIVTSFGRNISPEWIETMLLGDPRIAFCAVIGNGESYLTAILIPSAHGSAWF
EEEECCCCEEEEECCCCCCHHHHHHHHCCCCCEEEEEEEECCCCEEEEEEEECCCCCEEE
ASASEKNVHDMIAECCANAPDYAVPSAFILVSLDQARDAQILTSNFRFVRKTLASFVTNR
CCCCCCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCC
SLHMTDRSKNVS
CEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA