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The map label for this gene is appA [H]

Identifier: 182679492

GI number: 182679492

Start: 2905105

End: 2906709

Strand: Reverse

Name: appA [H]

Synonym: Bind_2547

Alternate gene names: 182679492

Gene position: 2906709-2905105 (Counterclockwise)

Preceding gene: 182679496

Following gene: 182679491

Centisome position: 69.7

GC content: 56.26

Gene sequence:

>1605_bases
ATGCACACTTCCTTTTTCCGCCCCACCCGGCGTGAACTCCTGGCCTATTCCGGCGCTTTGGCGGGATGCGCTAGCATTGC
ATCTCCCCGGCGGCTTATGGCAGCGCCGAAGCGCGGCGGCAGTTTGTCTCTGCTCGTCTCTGTCGAGCCGCAGGCTTTGA
CCGGCATTGCCACGACGGCAAATCCGGTCCTTGCGGCCAAGGTCAATGAGGGATTGCTCACCTATGATTTTGATCTTTCG
CCCCGGCCGCAGCTCGCTGTGGCGTGGACCGTCTCGGAGGATGGCCTTGCCTATACGTTCAGGCTACGCCCTGGCGTCAA
ATGGCATGATGGCAAGCCTTTCACTTCTGAGGATGTTGCCTTCTCTCTGACGACGCTCAAGGAAGTGCATCCGCGCGCCC
GAGGCACCTTCCTCAATCTTGTCGAGGTGCGCACGCCCGACCCTTTGGTGGTGCAATTGCGCCTGGCGAAACCTGCCCCC
TATCTTTTGACGGCATTCGCCGCCGAGGAAACGCCGATCTTTCCCAAGCATATCTATGAGCACGGACGGGTCGAAACCAA
TCCAGCCAACAATAGCCCGATCGGCACGGGGCCTTTTCGCTTCAAGGAATGGGTGCGCGGAAGCCATGTCATCTATGAAC
GCAATCCCGATTATTGGGATGAAGGCAAACCCTATATCGATAGACTGATCGTGCGCATCATTCCCGACAGCGCCGCGCGG
GTGACAGCGCTCGAGACCGGCGAGGTTCATATTACCGCGAGCACCTCGGTCCCGATGAGCGATGCGGATCGTCTCAAAAC
CTCGCCCGAATTAGCCTTCGAGAGCAATGGCTATCAATATATGAACTCTATTTCGCGGATCGAGTTCAACCTCGACCGGC
CCGCCTTCAAGGATGTCCGTGTCCGCCGCGCCTTCGCCCATGTCATCGACCGGACCGTCATCCGCGATGTCGCCAATTAC
GGCTATGGTACGCCGATTCCGGGCCCAATCAGCGTGACTTTGAGCAAATTCTTTTATCCTGAACTGCCTGTCTATGACAC
CGATACGAAAAAGGCGGAAGGCCTTCTCGATGCGGCGGGTTTCCCGCGCGGCGAAGGCGGCATCCGTTTACGCGTGACTC
ATGATTATTTGCCGGGCGGCGATTATTACAGACGTTCCGCCGATTATCTCAAACAGGCGCTGGCCAAGGTCGGCATCGCG
GTCCAAATCCGATCGCAAGATTTCGCAGCCTATACGAAGCGCGTTTATACCGATCGTGATTTCGATTTTACGATCAATGG
CATGAGCAATCTTTTCGATCCCACCGTCGGGGTGCAGCGGCTCTACTGGTCGAAGAATTTCAAGCCGGGCGTGCCCTTTT
CCAATGGGTCGCATTATGTCAATCCGGTGGTCGATCGACTGCTGGAAGAAGCCGCCGTCGAAATCGACCCACGCAAGCGC
TATGATCAGTTTGTCGCCTTCCAAAAACTCATAGCTGAAGATGTTCCTGATATCAGCATTCTCGGTGGCTCCGAATTTAC
CATCTACAATCGCAAGATTTCCGGCCATACAATAGGCGCAGAAGGCGTCTTCGGGAACTTCGCCGATATTCATTTCGTGG
GTTGA

Upstream 100 bases:

>100_bases
CGGCGCATAAATATTGATAAAATCTATCGATATAGTCAATTTTAAATACGGCCATCGCCAAGGATTGAAGCCCGCTCGCA
TGGGCCGAGGATTGACGCCG

Downstream 100 bases:

>100_bases
GGCCGAGCCGCTATGATCATCGACCGACGTCTGTTCAATCAGATGCTGCTCCTTGCCGGAAGCAATCTGTCTTTCCCTCA
CCCTGCTTCCGCAGCAAATG

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 534; Mature: 534

Protein sequence:

>534_residues
MHTSFFRPTRRELLAYSGALAGCASIASPRRLMAAPKRGGSLSLLVSVEPQALTGIATTANPVLAAKVNEGLLTYDFDLS
PRPQLAVAWTVSEDGLAYTFRLRPGVKWHDGKPFTSEDVAFSLTTLKEVHPRARGTFLNLVEVRTPDPLVVQLRLAKPAP
YLLTAFAAEETPIFPKHIYEHGRVETNPANNSPIGTGPFRFKEWVRGSHVIYERNPDYWDEGKPYIDRLIVRIIPDSAAR
VTALETGEVHITASTSVPMSDADRLKTSPELAFESNGYQYMNSISRIEFNLDRPAFKDVRVRRAFAHVIDRTVIRDVANY
GYGTPIPGPISVTLSKFFYPELPVYDTDTKKAEGLLDAAGFPRGEGGIRLRVTHDYLPGGDYYRRSADYLKQALAKVGIA
VQIRSQDFAAYTKRVYTDRDFDFTINGMSNLFDPTVGVQRLYWSKNFKPGVPFSNGSHYVNPVVDRLLEEAAVEIDPRKR
YDQFVAFQKLIAEDVPDISILGGSEFTIYNRKISGHTIGAEGVFGNFADIHFVG

Sequences:

>Translated_534_residues
MHTSFFRPTRRELLAYSGALAGCASIASPRRLMAAPKRGGSLSLLVSVEPQALTGIATTANPVLAAKVNEGLLTYDFDLS
PRPQLAVAWTVSEDGLAYTFRLRPGVKWHDGKPFTSEDVAFSLTTLKEVHPRARGTFLNLVEVRTPDPLVVQLRLAKPAP
YLLTAFAAEETPIFPKHIYEHGRVETNPANNSPIGTGPFRFKEWVRGSHVIYERNPDYWDEGKPYIDRLIVRIIPDSAAR
VTALETGEVHITASTSVPMSDADRLKTSPELAFESNGYQYMNSISRIEFNLDRPAFKDVRVRRAFAHVIDRTVIRDVANY
GYGTPIPGPISVTLSKFFYPELPVYDTDTKKAEGLLDAAGFPRGEGGIRLRVTHDYLPGGDYYRRSADYLKQALAKVGIA
VQIRSQDFAAYTKRVYTDRDFDFTINGMSNLFDPTVGVQRLYWSKNFKPGVPFSNGSHYVNPVVDRLLEEAAVEIDPRKR
YDQFVAFQKLIAEDVPDISILGGSEFTIYNRKISGHTIGAEGVFGNFADIHFVG
>Mature_534_residues
MHTSFFRPTRRELLAYSGALAGCASIASPRRLMAAPKRGGSLSLLVSVEPQALTGIATTANPVLAAKVNEGLLTYDFDLS
PRPQLAVAWTVSEDGLAYTFRLRPGVKWHDGKPFTSEDVAFSLTTLKEVHPRARGTFLNLVEVRTPDPLVVQLRLAKPAP
YLLTAFAAEETPIFPKHIYEHGRVETNPANNSPIGTGPFRFKEWVRGSHVIYERNPDYWDEGKPYIDRLIVRIIPDSAAR
VTALETGEVHITASTSVPMSDADRLKTSPELAFESNGYQYMNSISRIEFNLDRPAFKDVRVRRAFAHVIDRTVIRDVANY
GYGTPIPGPISVTLSKFFYPELPVYDTDTKKAEGLLDAAGFPRGEGGIRLRVTHDYLPGGDYYRRSADYLKQALAKVGIA
VQIRSQDFAAYTKRVYTDRDFDFTINGMSNLFDPTVGVQRLYWSKNFKPGVPFSNGSHYVNPVVDRLLEEAAVEIDPRKR
YDQFVAFQKLIAEDVPDISILGGSEFTIYNRKISGHTIGAEGVFGNFADIHFVG

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=470, Percent_Identity=24.8936170212766, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1789966, Length=462, Percent_Identity=25.974025974026, Blast_Score=135, Evalue=6e-33,
Organism=Escherichia coli, GI1787762, Length=436, Percent_Identity=26.1467889908257, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1787495, Length=533, Percent_Identity=22.7016885553471, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1789887, Length=420, Percent_Identity=26.6666666666667, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1789397, Length=473, Percent_Identity=24.1014799154334, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI87081878, Length=471, Percent_Identity=24.203821656051, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1787551, Length=569, Percent_Identity=22.4956063268893, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87082063, Length=425, Percent_Identity=22.8235294117647, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59520; Mature: 59520

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTSFFRPTRRELLAYSGALAGCASIASPRRLMAAPKRGGSLSLLVSVEPQALTGIATTA
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCEEEEEEECCHHHEEEECCC
NPVLAAKVNEGLLTYDFDLSPRPQLAVAWTVSEDGLAYTFRLRPGVKWHDGKPFTSEDVA
CCEEEEEECCCEEEEECCCCCCCCEEEEEEECCCCEEEEEEECCCCEECCCCCCCCCCCE
FSLTTLKEVHPRARGTFLNLVEVRTPDPLVVQLRLAKPAPYLLTAFAAEETPIFPKHIYE
EEEHHHHHHCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCEEEEEECCCCCCCHHHHHH
HGRVETNPANNSPIGTGPFRFKEWVRGSHVIYERNPDYWDEGKPYIDRLIVRIIPDSAAR
CCCEECCCCCCCCCCCCCHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCE
VTALETGEVHITASTSVPMSDADRLKTSPELAFESNGYQYMNSISRIEFNLDRPAFKDVR
EEEEECCEEEEEEECCCCCCCHHHHCCCCCEEECCCCHHHHHCHHEEEEECCCCCHHHHH
VRRAFAHVIDRTVIRDVANYGYGTPIPGPISVTLSKFFYPELPVYDTDTKKAEGLLDAAG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHCCCCCCEECCCCHHHCCHHHHCC
FPRGEGGIRLRVTHDYLPGGDYYRRSADYLKQALAKVGIAVQIRSQDFAAYTKRVYTDRD
CCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHCCCC
FDFTINGMSNLFDPTVGVQRLYWSKNFKPGVPFSNGSHYVNPVVDRLLEEAAVEIDPRKR
CCEEECCHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHH
YDQFVAFQKLIAEDVPDISILGGSEFTIYNRKISGHTIGAEGVFGNFADIHFVG
HHHHHHHHHHHHHCCCCEEEECCCEEEEEEEEECCCEECCCCCCCCCCEEEEEC
>Mature Secondary Structure
MHTSFFRPTRRELLAYSGALAGCASIASPRRLMAAPKRGGSLSLLVSVEPQALTGIATTA
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCEEEEEEECCHHHEEEECCC
NPVLAAKVNEGLLTYDFDLSPRPQLAVAWTVSEDGLAYTFRLRPGVKWHDGKPFTSEDVA
CCEEEEEECCCEEEEECCCCCCCCEEEEEEECCCCEEEEEEECCCCEECCCCCCCCCCCE
FSLTTLKEVHPRARGTFLNLVEVRTPDPLVVQLRLAKPAPYLLTAFAAEETPIFPKHIYE
EEEHHHHHHCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCEEEEEECCCCCCCHHHHHH
HGRVETNPANNSPIGTGPFRFKEWVRGSHVIYERNPDYWDEGKPYIDRLIVRIIPDSAAR
CCCEECCCCCCCCCCCCCHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCE
VTALETGEVHITASTSVPMSDADRLKTSPELAFESNGYQYMNSISRIEFNLDRPAFKDVR
EEEEECCEEEEEEECCCCCCCHHHHCCCCCEEECCCCHHHHHCHHEEEEECCCCCHHHHH
VRRAFAHVIDRTVIRDVANYGYGTPIPGPISVTLSKFFYPELPVYDTDTKKAEGLLDAAG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHCCCCCCEECCCCHHHCCHHHHCC
FPRGEGGIRLRVTHDYLPGGDYYRRSADYLKQALAKVGIAVQIRSQDFAAYTKRVYTDRD
CCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHCCCC
FDFTINGMSNLFDPTVGVQRLYWSKNFKPGVPFSNGSHYVNPVVDRLLEEAAVEIDPRKR
CCEEECCHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHH
YDQFVAFQKLIAEDVPDISILGGSEFTIYNRKISGHTIGAEGVFGNFADIHFVG
HHHHHHHHHHHHHCCCCEEEECCCEEEEEEEEECCCEECCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]