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The map label for this gene is gsiB [H]

Identifier: 182679491

GI number: 182679491

Start: 2903497

End: 2905092

Strand: Reverse

Name: gsiB [H]

Synonym: Bind_2546

Alternate gene names: 182679491

Gene position: 2905092-2903497 (Counterclockwise)

Preceding gene: 182679492

Following gene: 182679485

Centisome position: 69.66

GC content: 53.95

Gene sequence:

>1596_bases
ATGATCATCGACCGACGTCTGTTCAATCAGATGCTGCTCCTTGCCGGAAGCAATCTGTCTTTCCCTCACCCTGCTTCCGC
AGCAAATGATCAGCCTGTCCGCGGAGGCACGCTCAAGGTCGTTTACTATCCGGAGCCGAACCAGGTCGTCGGCATTAATA
CGAGCGCGGGTGGGCCCGCGACCATTGGCCCAAAAATTTTCGATGGCCTCCTCACCTATGATTACGCACTCAATCCACAA
CCGGCGCTCGCCACAGAATGGTCGATCGCTCCGGACGGGCGGGTCTTGACCTTTACACTCCGCAAAGGCGTGAAATTCAG
CGATGGCCATGATCTGACCTCGGAGGATGTCGCGTTTTCGATCAGCCGCCTGCGTGAAGCGCATCCGCGCGGCCGCATCA
CTTTCCAGAATGTCACCGAGATCGAGACGGCCGATCCTCATATCGTCAAGATCACATTGTCGAAGCCTTCGGCGCCGTTG
CTCCTGGCGTTGGCTGCTTCGGAATCCCCCATCGTGCCCAAGCATATTTTCGAACGCCTGAAGCCGAACGATGATCCAGC
CTTCGATCAGATCATCGGCAGTGGCCCATTCCTACTCAAGGAATGGGTAAAAGGAAGCCATATCCTTCTCGCGCGTAATC
CTGCCTATTGGGATGCGCCGAAGCCTTATCTCGATCGTATTATTTTCCGCTTCGTCTCAGATCCGGCCGCGCGCGCCGCC
GTGCTCGAAGCCGGTGAAGGCGACATCGGGCCGAACGCCGTTGCTTTCAGCGATCTCGAACGTTTCGAGGCTCTGCCGCA
ATTTACCGTGGATACAACTGTATTCGCCTATGGCGGCCCGCTGCAACAACTCATTCTCAATCTCGACAATGATTATCTGA
AGAATTTGAAAGTCCGCCAGGCCATTGCGCATGCAATCGATCTCGAACAACTCAATGCGATCGTCTTTTATGGCTATGGA
CAAGTCTCTCCGACGCCGATCAGTGTCGTAAACACCAAATATTTCGATCCCACGATCAAGGCGGCGACTTTCGACCCGAA
GCTTGCGAACCAATTACTTGATGAAGCCGGATACAAGCGGGGGGCCGGCGGCTTCCGCTTCAAGCTGCGGCTCACCAATA
ACCCCTATAATCCCTCCGCCTATTCCGATTTCCTGAAACAGGCTCTGGCCAGGATCGGCATCGACGCGACCATCCAGAAA
TTCGATTTCGGATCTTATGTAAAGACGGTTTACACCGATGGCGCCTTCGATCTTTCGACCGAATCTTGCGGTAATTTCTT
TGACCCCAGCGCTGGCGCCCAGCGTCTCTATTGGTCGAAGAACATCAAAAAGGGGCTGCCTTTTTCGAACGGCGCCCATT
ATGTCAATCCACAACTCGATCAGATTCTCGAAGATGCTGCCAGTACACTCGACGAGAGCCGACGACGGACGCTCTTCTTC
GAATTTCAGGAAATCATCGCGAGGGACCTGCCGATTATCAATTTGATCGCGCCACCGACCATCATCATCGCTAGAAAAAG
CGTGAAGAATTATGCAACCGGTGGCGATGGCTTCCTCGGCAATTTCGCCGAAACCTATATCGATACGAAAAGTTAA

Upstream 100 bases:

>100_bases
TACCATCTACAATCGCAAGATTTCCGGCCATACAATAGGCGCAGAAGGCGTCTTCGGGAACTTCGCCGATATTCATTTCG
TGGGTTGAGGCCGAGCCGCT

Downstream 100 bases:

>100_bases
TTCGTTCGTCTCTGGTATCTCAGTCTGCAGCAGTTCGAGGATCAACACATCCGGCAGGCGGGCAAACCACAGCTTGATCG
GTGTCTACTCAAGGGAGCAA

Product: extracellular solute-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 531; Mature: 531

Protein sequence:

>531_residues
MIIDRRLFNQMLLLAGSNLSFPHPASAANDQPVRGGTLKVVYYPEPNQVVGINTSAGGPATIGPKIFDGLLTYDYALNPQ
PALATEWSIAPDGRVLTFTLRKGVKFSDGHDLTSEDVAFSISRLREAHPRGRITFQNVTEIETADPHIVKITLSKPSAPL
LLALAASESPIVPKHIFERLKPNDDPAFDQIIGSGPFLLKEWVKGSHILLARNPAYWDAPKPYLDRIIFRFVSDPAARAA
VLEAGEGDIGPNAVAFSDLERFEALPQFTVDTTVFAYGGPLQQLILNLDNDYLKNLKVRQAIAHAIDLEQLNAIVFYGYG
QVSPTPISVVNTKYFDPTIKAATFDPKLANQLLDEAGYKRGAGGFRFKLRLTNNPYNPSAYSDFLKQALARIGIDATIQK
FDFGSYVKTVYTDGAFDLSTESCGNFFDPSAGAQRLYWSKNIKKGLPFSNGAHYVNPQLDQILEDAASTLDESRRRTLFF
EFQEIIARDLPIINLIAPPTIIIARKSVKNYATGGDGFLGNFAETYIDTKS

Sequences:

>Translated_531_residues
MIIDRRLFNQMLLLAGSNLSFPHPASAANDQPVRGGTLKVVYYPEPNQVVGINTSAGGPATIGPKIFDGLLTYDYALNPQ
PALATEWSIAPDGRVLTFTLRKGVKFSDGHDLTSEDVAFSISRLREAHPRGRITFQNVTEIETADPHIVKITLSKPSAPL
LLALAASESPIVPKHIFERLKPNDDPAFDQIIGSGPFLLKEWVKGSHILLARNPAYWDAPKPYLDRIIFRFVSDPAARAA
VLEAGEGDIGPNAVAFSDLERFEALPQFTVDTTVFAYGGPLQQLILNLDNDYLKNLKVRQAIAHAIDLEQLNAIVFYGYG
QVSPTPISVVNTKYFDPTIKAATFDPKLANQLLDEAGYKRGAGGFRFKLRLTNNPYNPSAYSDFLKQALARIGIDATIQK
FDFGSYVKTVYTDGAFDLSTESCGNFFDPSAGAQRLYWSKNIKKGLPFSNGAHYVNPQLDQILEDAASTLDESRRRTLFF
EFQEIIARDLPIINLIAPPTIIIARKSVKNYATGGDGFLGNFAETYIDTKS
>Mature_531_residues
MIIDRRLFNQMLLLAGSNLSFPHPASAANDQPVRGGTLKVVYYPEPNQVVGINTSAGGPATIGPKIFDGLLTYDYALNPQ
PALATEWSIAPDGRVLTFTLRKGVKFSDGHDLTSEDVAFSISRLREAHPRGRITFQNVTEIETADPHIVKITLSKPSAPL
LLALAASESPIVPKHIFERLKPNDDPAFDQIIGSGPFLLKEWVKGSHILLARNPAYWDAPKPYLDRIIFRFVSDPAARAA
VLEAGEGDIGPNAVAFSDLERFEALPQFTVDTTVFAYGGPLQQLILNLDNDYLKNLKVRQAIAHAIDLEQLNAIVFYGYG
QVSPTPISVVNTKYFDPTIKAATFDPKLANQLLDEAGYKRGAGGFRFKLRLTNNPYNPSAYSDFLKQALARIGIDATIQK
FDFGSYVKTVYTDGAFDLSTESCGNFFDPSAGAQRLYWSKNIKKGLPFSNGAHYVNPQLDQILEDAASTLDESRRRTLFF
EFQEIIARDLPIINLIAPPTIIIARKSVKNYATGGDGFLGNFAETYIDTKS

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=505, Percent_Identity=25.7425742574257, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI1787052, Length=458, Percent_Identity=26.6375545851528, Blast_Score=141, Evalue=8e-35,
Organism=Escherichia coli, GI1787762, Length=433, Percent_Identity=27.4826789838337, Blast_Score=131, Evalue=9e-32,
Organism=Escherichia coli, GI1789887, Length=504, Percent_Identity=24.0079365079365, Blast_Score=105, Evalue=9e-24,
Organism=Escherichia coli, GI1787495, Length=486, Percent_Identity=23.2510288065844, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1787551, Length=511, Percent_Identity=22.8962818003914, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1789397, Length=516, Percent_Identity=23.062015503876, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87082063, Length=291, Percent_Identity=24.3986254295533, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 58484; Mature: 58484

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIDRRLFNQMLLLAGSNLSFPHPASAANDQPVRGGTLKVVYYPEPNQVVGINTSAGGPA
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEECCCCCCC
TIGPKIFDGLLTYDYALNPQPALATEWSIAPDGRVLTFTLRKGVKFSDGHDLTSEDVAFS
CCCHHHHHHHHHEEECCCCCCCCCCCCEECCCCEEEEEEECCCCEECCCCCCCHHHHHHH
ISRLREAHPRGRITFQNVTEIETADPHIVKITLSKPSAPLLLALAASESPIVPKHIFERL
HHHHHHCCCCCCEEECCCCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHC
KPNDDPAFDQIIGSGPFLLKEWVKGSHILLARNPAYWDAPKPYLDRIIFRFVSDPAARAA
CCCCCHHHHHHHCCCCHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCHHHHHH
VLEAGEGDIGPNAVAFSDLERFEALPQFTVDTTVFAYGGPLQQLILNLDNDYLKNLKVRQ
EEECCCCCCCCCCHHHHHHHHHHHCCCCEECEEEEECCCCHHHHHHHCCHHHHHHHHHHH
AIAHAIDLEQLNAIVFYGYGQVSPTPISVVNTKYFDPTIKAATFDPKLANQLLDEAGYKR
HHHHHHCHHHCCEEEEEECCCCCCCCEEEEECEECCCCCEEEECCHHHHHHHHHHCCCCC
GAGGFRFKLRLTNNPYNPSAYSDFLKQALARIGIDATIQKFDFGSYVKTVYTDGAFDLST
CCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHCCCCCCCCCH
ESCGNFFDPSAGAQRLYWSKNIKKGLPFSNGAHYVNPQLDQILEDAASTLDESRRRTLFF
HHCCCCCCCCCCCHHEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
EFQEIIARDLPIINLIAPPTIIIARKSVKNYATGGDGFLGNFAETYIDTKS
HHHHHHHHCCCEEEEECCCEEEEEEHHHHHCCCCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure
MIIDRRLFNQMLLLAGSNLSFPHPASAANDQPVRGGTLKVVYYPEPNQVVGINTSAGGPA
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEECCCCCCC
TIGPKIFDGLLTYDYALNPQPALATEWSIAPDGRVLTFTLRKGVKFSDGHDLTSEDVAFS
CCCHHHHHHHHHEEECCCCCCCCCCCCEECCCCEEEEEEECCCCEECCCCCCCHHHHHHH
ISRLREAHPRGRITFQNVTEIETADPHIVKITLSKPSAPLLLALAASESPIVPKHIFERL
HHHHHHCCCCCCEEECCCCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHC
KPNDDPAFDQIIGSGPFLLKEWVKGSHILLARNPAYWDAPKPYLDRIIFRFVSDPAARAA
CCCCCHHHHHHHCCCCHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCHHHHHH
VLEAGEGDIGPNAVAFSDLERFEALPQFTVDTTVFAYGGPLQQLILNLDNDYLKNLKVRQ
EEECCCCCCCCCCHHHHHHHHHHHCCCCEECEEEEECCCCHHHHHHHCCHHHHHHHHHHH
AIAHAIDLEQLNAIVFYGYGQVSPTPISVVNTKYFDPTIKAATFDPKLANQLLDEAGYKR
HHHHHHCHHHCCEEEEEECCCCCCCCEEEEECEECCCCCEEEECCHHHHHHHHHHCCCCC
GAGGFRFKLRLTNNPYNPSAYSDFLKQALARIGIDATIQKFDFGSYVKTVYTDGAFDLST
CCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHCCCCCCCCCH
ESCGNFFDPSAGAQRLYWSKNIKKGLPFSNGAHYVNPQLDQILEDAASTLDESRRRTLFF
HHCCCCCCCCCCCHHEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
EFQEIIARDLPIINLIAPPTIIIARKSVKNYATGGDGFLGNFAETYIDTKS
HHHHHHHHCCCEEEEECCCEEEEEEHHHHHCCCCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA