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The map label for this gene is rfbB [C]

Identifier: 182679463

GI number: 182679463

Start: 2871072

End: 2872145

Strand: Reverse

Name: rfbB [C]

Synonym: Bind_2516

Alternate gene names: 182679463

Gene position: 2872145-2871072 (Counterclockwise)

Preceding gene: 182679464

Following gene: 182679461

Centisome position: 68.87

GC content: 48.7

Gene sequence:

>1074_bases
ATGAGACTGTTGGAGACAAACAAGGACGTGCAGGTGGTGTTGTTCGACTGCAATCCTGATATGCGGAGGATTACCGGATT
CAATAAAGGACCGAGTGATCCGCAAGATCCAAAATATGATCCGAAAGATCGCTATAATACCGTAGCGGATCGGGTGACTT
TTGTCCAAGGCGATCTGGCTTTGTTGTCACACGTACTTGCAGTTTTTGACAAGCATGAACCGACGAGTGTCTTCCATCTC
GGTGCTTTACTCTCAGCAGGTGCAGATGCAAACCCCACTCTTGGTTTTGATGTAGACCTCTTGGGTACACGGCATGTCTT
CGAAGCCGCTCGTATCTATTGCCAACATAATAAAAAACCACCCGTTCGCGTATTGTTTCCCAGCACAATTGCTTCTTTCG
GGCTATTTCCTTTCACGGATCCAAATGACATACATAAGCTAGTTGTCAGACCACCCAAGCCGGAAGACAAACTCGTTGTG
CCCAACGAGGCCATTCAATTACCAACGACCATGTATGGGGTGTCGAAAGTCTCGGTGGAACGATTGGGAGAACATTATCA
TGCAAAGGGCTGGATAGACTTCTGGGCTGTCCGATTTCCGTCTGTAGTGGGTGCAGCGCGGGGTCCTGGAGGAACGACTG
CTTATTCCACTCTTATGATCCAGGAACCATTGATGGATAAGGCTTATGAAGTCTACGTATCGTCCGATACACGCCTCGAT
ATCATTTATGTCAAAGACGCCGTCGATGCCTTGATCAGGCTACATGATGCGAAAGACCTCAAGCTACGCCGCGTCTATAA
CATCGCTGGTATTCGAATAGGAGGTGAAGCGCCCCAGGCATCTGATATCGCGGCAGCTGTAAAACTGGTCAAGCCAAACG
CCGCTGCAATTACATACAAACAGAATGACCAGCTCACGGCGATCGTTCATACCTTTGGAATTCTCGATGCCAAAGCGACG
TACGACGACTTGCAATGGGCGGGGCCGACCTTCGATCTCAAACAAACGATCATCGATTGCAAGTCTGAGATTGATCAATA
TCCTGGCCGGATTAAAGCGATCGAGCTTTACTGA

Upstream 100 bases:

>100_bases
CTTCTATGTAACTAAATAGTGGAGTTAGCAATGGGCGACCAATATAGACAATTGATCCTTATCACGGGTGGGACTGGTTT
TTATCGCATCTTATCTTGCG

Downstream 100 bases:

>100_bases
ATAACCTAGTCATCGACAACCACACCTGTAATGGGCATCGCAATACTCTAAGGCAGGGAGGTTGTCGATTTTATTGAGCG
AAATCAACACAAAGTTAGGT

Product: NAD-dependent epimerase/dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MRLLETNKDVQVVLFDCNPDMRRITGFNKGPSDPQDPKYDPKDRYNTVADRVTFVQGDLALLSHVLAVFDKHEPTSVFHL
GALLSAGADANPTLGFDVDLLGTRHVFEAARIYCQHNKKPPVRVLFPSTIASFGLFPFTDPNDIHKLVVRPPKPEDKLVV
PNEAIQLPTTMYGVSKVSVERLGEHYHAKGWIDFWAVRFPSVVGAARGPGGTTAYSTLMIQEPLMDKAYEVYVSSDTRLD
IIYVKDAVDALIRLHDAKDLKLRRVYNIAGIRIGGEAPQASDIAAAVKLVKPNAAAITYKQNDQLTAIVHTFGILDAKAT
YDDLQWAGPTFDLKQTIIDCKSEIDQYPGRIKAIELY

Sequences:

>Translated_357_residues
MRLLETNKDVQVVLFDCNPDMRRITGFNKGPSDPQDPKYDPKDRYNTVADRVTFVQGDLALLSHVLAVFDKHEPTSVFHL
GALLSAGADANPTLGFDVDLLGTRHVFEAARIYCQHNKKPPVRVLFPSTIASFGLFPFTDPNDIHKLVVRPPKPEDKLVV
PNEAIQLPTTMYGVSKVSVERLGEHYHAKGWIDFWAVRFPSVVGAARGPGGTTAYSTLMIQEPLMDKAYEVYVSSDTRLD
IIYVKDAVDALIRLHDAKDLKLRRVYNIAGIRIGGEAPQASDIAAAVKLVKPNAAAITYKQNDQLTAIVHTFGILDAKAT
YDDLQWAGPTFDLKQTIIDCKSEIDQYPGRIKAIELY
>Mature_357_residues
MRLLETNKDVQVVLFDCNPDMRRITGFNKGPSDPQDPKYDPKDRYNTVADRVTFVQGDLALLSHVLAVFDKHEPTSVFHL
GALLSAGADANPTLGFDVDLLGTRHVFEAARIYCQHNKKPPVRVLFPSTIASFGLFPFTDPNDIHKLVVRPPKPEDKLVV
PNEAIQLPTTMYGVSKVSVERLGEHYHAKGWIDFWAVRFPSVVGAARGPGGTTAYSTLMIQEPLMDKAYEVYVSSDTRLD
IIYVKDAVDALIRLHDAKDLKLRRVYNIAGIRIGGEAPQASDIAAAVKLVKPNAAAITYKQNDQLTAIVHTFGILDAKAT
YDDLQWAGPTFDLKQTIIDCKSEIDQYPGRIKAIELY

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Caenorhabditis elegans, GI32566934, Length=265, Percent_Identity=30.9433962264151, Blast_Score=117, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24667531, Length=232, Percent_Identity=31.8965517241379, Blast_Score=116, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 39662; Mature: 39662

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLETNKDVQVVLFDCNPDMRRITGFNKGPSDPQDPKYDPKDRYNTVADRVTFVQGDLA
CCCCCCCCCEEEEEECCCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHEEEECCHH
LLSHVLAVFDKHEPTSVFHLGALLSAGADANPTLGFDVDLLGTRHVFEAARIYCQHNKKP
HHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCC
PVRVLFPSTIASFGLFPFTDPNDIHKLVVRPPKPEDKLVVPNEAIQLPTTMYGVSKVSVE
CEEEECCHHHHHCCCCCCCCCCCHHEEEECCCCCCCCEECCCCCEECCHHHHHHHHHHHH
RLGEHYHAKGWIDFWAVRFPSVVGAARGPGGTTAYSTLMIQEPLMDKAYEVYVSSDTRLD
HHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCHHHEEEEECCHHHCCEEEEEECCCCEEE
IIYVKDAVDALIRLHDAKDLKLRRVYNIAGIRIGGEAPQASDIAAAVKLVKPNAAAITYK
EEEEHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCCCCCHHHHHHHEEECCCCEEEEEE
QNDQLTAIVHTFGILDAKATYDDLQWAGPTFDLKQTIIDCKSEIDQYPGRIKAIELY
CCCCEEEEEHHHHHCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEC
>Mature Secondary Structure
MRLLETNKDVQVVLFDCNPDMRRITGFNKGPSDPQDPKYDPKDRYNTVADRVTFVQGDLA
CCCCCCCCCEEEEEECCCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHEEEECCHH
LLSHVLAVFDKHEPTSVFHLGALLSAGADANPTLGFDVDLLGTRHVFEAARIYCQHNKKP
HHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCC
PVRVLFPSTIASFGLFPFTDPNDIHKLVVRPPKPEDKLVVPNEAIQLPTTMYGVSKVSVE
CEEEECCHHHHHCCCCCCCCCCCHHEEEECCCCCCCCEECCCCCEECCHHHHHHHHHHHH
RLGEHYHAKGWIDFWAVRFPSVVGAARGPGGTTAYSTLMIQEPLMDKAYEVYVSSDTRLD
HHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCHHHEEEEECCHHHCCEEEEEECCCCEEE
IIYVKDAVDALIRLHDAKDLKLRRVYNIAGIRIGGEAPQASDIAAAVKLVKPNAAAITYK
EEEEHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCCCCCHHHHHHHEEECCCCEEEEEE
QNDQLTAIVHTFGILDAKATYDDLQWAGPTFDLKQTIIDCKSEIDQYPGRIKAIELY
CCCCEEEEEHHHHHCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA